FastQCFastQC Report
Tue 24 May 2016
ERR841215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20123820
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG689910.3428325238448764No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG571970.2842253607913408No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC475440.23625733086461717No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG467330.2322272809039238Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA455230.2262145059933949No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA442900.2200874386672113No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC438510.21790594429884586No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA431740.2145417718902276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA428420.21289198571642956No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG393500.1955394154787709No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA390380.19398901401423785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA385700.19166341181743823No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG383130.19038631830338373No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG359520.1786539533746575No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT359030.1784104608369584No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG350150.17399777974559502No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA348210.1730337480657251No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT341410.16965466795071713No Hit
AAAAAAAAAAAAAAAAAAAAAAAA341090.1694956524158932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA340840.16937142152931203No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG321390.15970625855329654No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC317210.1576291181296593No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA302930.1505330498881425No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA298550.1483565247552403No Hit
AAGCAGTGGTATCAACGCAGAGTG291840.14502216775940155No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC283600.140927517737686No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA272040.1351830815421724Illumina Single End Adapter 1 (100% over 32bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT259770.1290858296287683No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG259330.12886718326838542No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT255370.1268993660249396Illumina Single End Adapter 2 (100% over 32bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG253440.12594030358053293No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT244440.1214679916636106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227460.11303022984701712No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT224350.1114847976179473No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC223410.11101768948440208No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA222730.11067978147290126No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC218490.10857282563648452No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA215270.10697273181731898Illumina Single End Adapter 1 (100% over 32bp)
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC212900.10579502301252942No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA211920.10530803793713123No Hit
AAGCAGTGGTATCAACGCAGAGCG203650.10119848020902593No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC201620.10018972540998677Illumina Single End Adapter 2 (100% over 25bp)
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT201540.1001499715262808No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA80150.0129.1632544
ATGTTCT50100.092.8394844
CTATACG5300.089.46364644
CGCTACC22200.082.8910844
ATTAGTC22750.080.39088444
ACGACAC12150.078.9797744
TTAAGCC61700.074.06795543
TTCAGGA78400.072.575144
CCAGAAT46550.070.8168144
TCGTGCG87750.067.8080943
ATCCTTG85900.067.807843
AATCCCG54700.065.9902643
AATCGCA5700.063.3794344
GTTCAGG140850.059.88942343
CGTGCGC54850.059.68903444
TCCTTGT75100.059.07800344
ATTAAGC79350.058.31229442
AACGACA23300.056.3350343
CCTTAAC32500.054.939443
CTATCAT22850.054.34751544
Produced by FastQC (version 0.11.5)