FastQCFastQC Report
Tue 24 May 2016
ERR841214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156310
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG6610.42287761499584153No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC6570.42031859765849916No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA5570.3563431642249376No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC5180.33139274518584866No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC4510.2885292047853624No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG4310.2757341180986501No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCGC3920.25078369905956116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA3610.23095131469515706No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT3610.23095131469515706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.21751647367410915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA3380.21623696500543793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3360.2149574563367667No Hit
TAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCA3320.21239843899942423No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC3200.20472138698739684No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG3180.2034418783187256No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG3110.19896359797837632Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.1816902309513147No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC2820.18041072228264346No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG2660.1701746529332736No Hit
AAAAAAAAAAAAAAAAAAAAAAAA2630.16825538993026679No Hit
AGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATAT2620.16761563559593115No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTTC2510.1605783379182394No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC2500.15993858358390378No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC2500.15993858358390378No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG2490.15929882924956815No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG2450.1567398119122257No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC2270.14522423389418462No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT2260.14458447955984902No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG2260.14458447955984902No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAG2150.13754718188215725No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC2150.13754718188215725No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACG1920.12283283219243811No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG1900.12155332352376687No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG1820.11643528884908196No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1810.11579553451474633Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG1800.11515578018041071No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG1800.11515578018041071No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCT1790.11451602584607512No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT1770.11323651717740388No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.10811848250271897No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1660.10619921949971212Illumina Single End Adapter 2 (100% over 34bp)
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC1580.10108118482502718No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT1570.10044143049069158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGAAT355.4569682E-11118.99029544
CGTGCGC650.0109.837244
TATGACC251.710732E-595.1922344
CTTAGCC1050.090.6592744
CGTGTGC208.438049E-489.24271444
CGACTTG150.001031625884.8753443
TCCTTGT800.081.80582444
AATCCCG1550.079.3995143
ATGTTCT304.227316E-579.3268644
TTCAGGA1400.076.4937644
CCCGTCA658.731149E-1173.2247944
ATCCCGC1000.071.3941744
TAGATAT250.002046337171.3941744
ATGCCTA250.002046337171.3941744
CCCCGTC501.72804E-1067.90027643
GTGTTAG800.063.65651343
TTAGATA200.003229984563.65651343
TCGTGCG800.063.65651343
TATATGA256.015225E-661.61890442
TACGCTA150.003678402261.61890442
Produced by FastQC (version 0.11.5)