##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841214.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156310
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.03818053867315	37.0	35.0	39.0	31.0	39.0
2	36.685541552044015	38.0	35.0	39.0	32.0	39.0
3	36.768568869554095	38.0	35.0	39.0	33.0	39.0
4	37.016838334079715	38.0	35.0	39.0	33.0	40.0
5	37.8170430554667	39.0	36.0	40.0	33.0	41.0
6	37.82704241571237	39.0	36.0	40.0	33.0	41.0
7	37.75423197492163	39.0	36.0	40.0	33.0	41.0
8	37.702699763290894	39.0	36.0	40.0	33.0	41.0
9	37.687518392937115	39.0	36.0	40.0	33.0	41.0
10	37.51667839549613	39.0	36.0	40.0	32.0	41.0
11	37.40478536242083	39.0	36.0	40.0	32.0	41.0
12	37.34984965773143	39.0	36.0	40.0	32.0	41.0
13	37.23694581280788	39.0	35.0	40.0	32.0	41.0
14	37.160776661761886	38.0	35.0	40.0	32.0	41.0
15	37.03375343867955	38.0	35.0	40.0	31.0	41.0
16	36.91717100633357	38.0	35.0	40.0	31.0	41.0
17	36.89334015737957	38.0	35.0	40.0	31.0	41.0
18	36.775874864052206	38.0	35.0	40.0	31.0	41.0
19	36.69250207920159	38.0	35.0	40.0	31.0	41.0
20	36.56822979975689	38.0	35.0	40.0	31.0	41.0
21	36.30437591964686	38.0	34.0	40.0	30.0	41.0
22	36.220971147079524	38.0	34.0	40.0	30.0	41.0
23	36.25267737188919	38.0	34.0	40.0	30.0	41.0
24	36.204164800716526	38.0	34.0	40.0	30.0	41.0
25	36.21609246095574	38.0	34.0	40.0	30.0	41.0
26	36.18170475439979	38.0	34.0	40.0	30.0	41.0
27	36.215001928268414	38.0	34.0	40.0	30.0	41.0
28	36.165057635431474	38.0	34.0	40.0	30.0	41.0
29	36.19178689284643	37.0	34.0	40.0	31.0	41.0
30	36.233168620478146	37.0	34.0	40.0	31.0	41.0
31	36.15598578520013	37.0	34.0	40.0	30.0	41.0
32	36.13284291598488	37.0	34.0	40.0	31.0	41.0
33	36.05222049445768	37.0	34.0	40.0	31.0	41.0
34	35.97622187155488	37.0	34.0	40.0	30.0	41.0
35	35.90562449449501	37.0	34.0	40.0	30.0	41.0
36	35.72223411558876	36.0	34.0	40.0	30.0	41.0
37	35.6707511032459	36.0	34.0	39.0	30.0	41.0
38	35.607675277868026	36.0	34.0	39.0	30.0	41.0
39	35.408453938053434	35.0	34.0	39.0	30.0	41.0
40	35.289982534445954	35.0	33.0	39.0	30.0	41.0
41	35.152258674333424	35.0	33.0	39.0	29.0	40.0
42	34.979444826428306	35.0	33.0	39.0	29.0	40.0
43	34.842573268476485	35.0	33.0	38.0	29.0	40.0
44	34.723177134931646	35.0	33.0	38.0	29.0	40.0
45	34.6423364415259	35.0	33.0	38.0	29.0	40.0
46	34.598857407550376	35.0	33.0	38.0	29.0	40.0
47	34.533488648141095	35.0	33.0	38.0	29.0	40.0
48	34.50772143070639	35.0	33.0	37.0	29.0	40.0
49	34.4110961649326	35.0	33.0	37.0	29.0	40.0
50	34.54297965696051	35.0	33.0	38.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	4.0
23	14.0
24	37.0
25	74.0
26	197.0
27	444.0
28	880.0
29	1834.0
30	3310.0
31	5588.0
32	8640.0
33	12421.0
34	16968.0
35	17193.0
36	20444.0
37	22393.0
38	20872.0
39	17400.0
40	7595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.06128846522935	17.691126607382763	30.247584927387884
2	26.959247648902824	27.331584671486148	26.780116435288846	18.92905124432218
3	33.11944213422046	19.599513786705906	23.632525110357623	23.64851896871601
4	21.129166400102363	21.767641225769303	27.441622416991873	29.661569957136457
5	24.675324675324674	23.464269720427357	25.079649414624782	26.780756189623183
6	28.215725161537968	21.160514362484804	24.267161410018552	26.356599065958676
7	31.30573859637899	20.40368498496577	23.976073187895846	24.31450323075939
8	29.540656387947028	18.67506877359094	26.773079137611155	25.011195700850873
9	28.378222762459217	21.67615635595931	23.24035570340989	26.705265178171583
10	28.290576418655238	21.541807945748833	26.138442837950226	24.029172797645703
11	31.332608278421088	22.102232742626832	24.105303563431644	22.45985541552044
12	29.102424668927128	19.685240867506877	24.100185528756956	27.11214893480903
13	26.558761435608723	21.754206384748258	23.019640458064103	28.667391721578916
14	28.9348090333312	22.299277077602202	20.35762267289361	28.408291216172987
15	25.914528820932762	23.64851896871601	22.779732582688247	27.657219627662975
16	26.645128270744035	23.264666368114646	24.309385196084705	25.780820165056618
17	30.459983366387306	20.419039088989827	19.259804235173693	29.861173309449175
18	26.795470539312905	22.569253406691832	20.694133452754144	29.941142601241122
19	27.056490307721837	20.903972874416223	25.063655556266397	26.975881261595546
20	29.46708463949843	20.0646151877679	23.97991171390186	26.488388458831807
21	30.45934361205297	23.467468492099034	21.509180474697718	24.564007421150276
22	28.44347770456145	22.472010747872815	23.074659330816967	26.00985221674877
23	28.343036274070755	22.79828545838398	22.734310024950418	26.12436824259484
24	31.272471370993536	23.43740003838526	19.858614292111827	25.431514298509374
25	27.190888808177245	23.283220048772897	22.02174025839257	27.504150884657292
26	30.96992382453375	23.30246913580247	21.03099553454163	24.696611505122142
27	28.868172932430813	22.39451041929864	23.56144527042302	25.17587137784752
28	27.92034116402687	22.73079436513444	23.645685258668895	25.7031792121698
29	29.728366105762678	21.564349415005317	24.562958518558922	24.144325960673086
30	28.84266821041895	22.580823012935817	23.672476412981304	24.90403236366393
31	27.399794737172815	21.11903149457869	26.92364083194281	24.557532936305687
32	26.24132483213903	21.211843367375728	24.610675393556395	27.93615640692885
33	25.14800918726907	21.769833231094054	27.25294948428606	25.829208097350815
34	24.912993666371243	21.535778877816448	29.38112016601457	24.170107289797745
35	29.450083431094658	20.288693447197954	23.647068253193336	26.61415486851405
36	27.258365753010043	21.818503945726878	22.10049976746418	28.8226305337989
37	24.564115200525674	23.774855700663814	26.280773280168457	25.380255818642055
38	29.922854314793685	22.756088162179726	21.251709521182953	26.06934800184364
39	27.30194931923068	23.90920014388379	24.865874285364416	23.92297625152111
40	27.480690859693386	22.712982728507665	22.073355326993983	27.73297108480497
41	25.769760938915365	25.11047221456422	22.46780355243984	26.651963294080577
42	25.855865939653654	24.75415340827324	25.94541006411999	23.444570587953116
43	24.498347449712934	26.53256782764753	22.806006025515053	26.16307869712448
44	22.907998150716598	24.410540915395284	24.326332474737466	28.355128459150652
45	23.765481405497212	21.586103100064744	28.593409734892756	26.055005759545292
46	24.65494908944303	21.922849275586092	24.061350009006922	29.360851625963956
47	27.83885055231245	22.650568257314706	23.43098087501882	26.079600315354018
48	24.580053800936536	22.027498256451132	25.67300986350503	27.7194380791073
49	27.884536195679026	21.398962308178	21.48818184313833	29.228319653004643
50	23.68767451136817	22.72636617471081	24.026725169525328	29.559234144395692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2664.0
1	2620.0
2	1486.0
3	395.0
4	389.0
5	308.0
6	169.5
7	110.0
8	114.0
9	111.0
10	108.0
11	106.0
12	99.5
13	105.5
14	132.5
15	160.0
16	179.5
17	208.0
18	239.0
19	279.0
20	319.5
21	355.0
22	421.0
23	528.0
24	645.5
25	730.0
26	809.0
27	899.5
28	944.5
29	1014.0
30	1130.5
31	1240.0
32	1353.5
33	1533.0
34	1820.0
35	2166.5
36	2448.0
37	2809.5
38	3292.5
39	3826.5
40	4357.5
41	5275.5
42	6447.0
43	7364.5
44	7809.0
45	7965.5
46	8061.0
47	8614.0
48	9714.0
49	10944.5
50	12553.5
51	14117.0
52	14922.5
53	14134.0
54	13297.5
55	13028.0
56	12243.5
57	12001.0
58	12287.0
59	11554.0
60	10578.0
61	10153.5
62	9650.5
63	9127.0
64	8590.0
65	7752.0
66	6824.0
67	6196.5
68	5702.0
69	5090.5
70	4460.5
71	3987.5
72	3640.0
73	3344.0
74	2806.0
75	2218.0
76	1883.5
77	1571.0
78	1232.0
79	968.0
80	732.5
81	548.5
82	414.5
83	323.5
84	271.0
85	230.0
86	175.5
87	107.5
88	56.5
89	36.0
90	24.0
91	16.0
92	12.5
93	11.0
94	6.5
95	3.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	2126.0
25	1904.0
26	1886.0
27	1961.0
28	1765.0
29	1567.0
30	1870.0
31	1447.0
32	1590.0
33	1411.0
34	1544.0
35	1776.0
36	1540.0
37	1576.0
38	1686.0
39	1836.0
40	1870.0
41	1877.0
42	1935.0
43	2015.0
44	2195.0
45	2356.0
46	3688.0
47	12519.0
48	27516.0
49	22714.0
50	50140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.926812104152006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.01974316908978	21.380589853496257
2	12.845316890120873	8.71601305098842
3	6.543342573211895	6.6598426204337535
4	4.18057362674662	5.673341436888235
5	3.051045614829062	5.175612564775126
6	2.1176293111576245	4.310664704753375
7	1.54249401293583	3.663233318405732
8	1.218155418528785	3.306250399846459
9	0.8862740661122739	2.7061608342396517
>10	4.2805151703720465	23.940246945173055
>50	0.18102619222718788	4.215981063271704
>100	0.1263411966585582	8.721131085663107
>500	0.0075427580094661615	1.530932122065127
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	661	0.42287761499584153	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	657	0.42031859765849916	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	557	0.3563431642249376	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	518	0.33139274518584866	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	451	0.2885292047853624	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	431	0.2757341180986501	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCGC	392	0.25078369905956116	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	361	0.23095131469515706	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	361	0.23095131469515706	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	340	0.21751647367410915	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	338	0.21623696500543793	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	336	0.2149574563367667	No Hit
TAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCA	332	0.21239843899942423	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	320	0.20472138698739684	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	318	0.2034418783187256	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	311	0.19896359797837632	Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	284	0.1816902309513147	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	282	0.18041072228264346	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	266	0.1701746529332736	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	263	0.16825538993026679	No Hit
AGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATAT	262	0.16761563559593115	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTTC	251	0.1605783379182394	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	250	0.15993858358390378	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	250	0.15993858358390378	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	249	0.15929882924956815	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	245	0.1567398119122257	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	227	0.14522423389418462	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	226	0.14458447955984902	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	226	0.14458447955984902	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAG	215	0.13754718188215725	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	215	0.13754718188215725	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACG	192	0.12283283219243811	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	190	0.12155332352376687	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	182	0.11643528884908196	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	181	0.11579553451474633	Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	180	0.11515578018041071	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	180	0.11515578018041071	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCT	179	0.11451602584607512	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	177	0.11323651717740388	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	169	0.10811848250271897	No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	166	0.10619921949971212	Illumina Single End Adapter 2 (100% over 34bp)
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	158	0.10108118482502718	No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	157	0.10044143049069158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.41264154564647176	0.0	0.0	0.0	0.0
2	1.2104152005629838	0.0	0.0	0.0	0.0
3	1.610901413857079	0.0	0.0	0.0	0.0
4	1.9243810376815302	0.0	0.0	0.0	0.0
5	2.511675516601625	0.0	0.0	0.0	0.0
6	2.747744865971467	0.0	0.0	0.0	0.0
7	2.896807625871665	0.0	0.0	0.0	0.0
8	3.022199475401446	0.0	0.0	0.0	0.0
9	3.1437527989252128	0.0	0.0	0.0	0.0
10	3.2441942294159043	0.0	0.0	0.0	0.0
11	3.3625487812679933	0.0	0.0	0.0	0.0
12	3.429722986373233	0.0	0.0	0.0	0.0
13	3.5007357174844858	0.0	0.0	0.0	0.0
14	3.5781459919390954	0.0	0.0	0.0	0.0
15	3.7073763674748896	0.0	0.0	0.0	0.0
16	3.7719915552427867	0.0	0.0	0.0	0.0
17	3.844923549357047	0.0	0.0	0.0	0.0
18	3.9044207024502593	0.0	0.0	0.0	0.0
19	4.143688823491779	0.0	0.0	0.0	0.0
20	4.214061800268697	0.0	0.0	0.0	0.0
21	4.3253790544430935	0.0	0.0	0.0	0.0
22	4.365683577506237	0.0	0.0	0.0	0.0
23	4.40918687224106	0.0	0.0	0.0	0.0
24	4.443093851960847	0.0	0.0	0.0	0.0
25	4.4949139530420315	0.0	0.0	0.0	0.0
26	4.636939415264538	0.0	0.0	0.0	0.0
27	4.663809097306634	0.0	0.0	0.0	0.0
28	4.692598042351737	0.0	0.0	0.0	0.0
29	4.724585759068518	0.0	0.0	0.0	0.0
30	4.75785298445397	0.0	0.0	0.0	0.0
31	4.789840701170751	0.0	0.0	0.0	0.0
32	4.816710383212846	0.0	0.0	0.0	0.0
33	4.848058345595291	0.0	0.0	0.0	0.0
34	4.869809992962702	0.0	0.0	0.0	0.0
35	4.889002622992771	0.0	0.0	0.0	0.0
36	4.91395304203186	0.0	0.0	0.0	0.0
37	4.933145672061928	0.0	0.0	0.0	0.0
38	4.949139530420319	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGAAT	35	5.4569682E-11	118.990295	44
CGTGCGC	65	0.0	109.8372	44
TATGACC	25	1.710732E-5	95.19223	44
CTTAGCC	105	0.0	90.65927	44
CGTGTGC	20	8.438049E-4	89.242714	44
CGACTTG	15	0.0010316258	84.87534	43
TCCTTGT	80	0.0	81.805824	44
AATCCCG	155	0.0	79.39951	43
ATGTTCT	30	4.227316E-5	79.32686	44
TTCAGGA	140	0.0	76.49376	44
CCCGTCA	65	8.731149E-11	73.22479	44
ATCCCGC	100	0.0	71.39417	44
TAGATAT	25	0.0020463371	71.39417	44
ATGCCTA	25	0.0020463371	71.39417	44
CCCCGTC	50	1.72804E-10	67.900276	43
GTGTTAG	80	0.0	63.656513	43
TTAGATA	20	0.0032299845	63.656513	43
TCGTGCG	80	0.0	63.656513	43
TATATGA	25	6.015225E-6	61.618904	42
TACGCTA	15	0.0036784022	61.618904	42
>>END_MODULE