FastQCFastQC Report
Tue 24 May 2016
ERR841213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9723432
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1182341.2159698345193344No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC920990.9471861375695331No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC494530.50859614177381No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC435460.44784598689022564No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG406820.41839136634060897No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA359880.370116230565504No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC298000.3064761495735251No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA286010.29414511254873793No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG280680.2886635089338826No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA279630.2875836433061907No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT263620.2711182635925258No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG249260.25634981557951964No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC204210.21001843793426025No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG198430.2040740347646798No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG186310.19160930009074983No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG176210.18122202119580821No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC168530.17332357546183283No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG157390.1618667153737487No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC145630.14977222034359886No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG144690.14880548349595082No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG144450.14855865706676408No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA135360.13921010606131662No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT129600.13328627176083505No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT124400.12793836579512255No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT124120.12765040162773802No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA123280.12678650912558445No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG119000.12238477113842108No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT118110.12146945646352028No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT116550.11986508467380654No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC111820.11500054713191803No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT111190.11435262775530287No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA108540.11162725259969937No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC108190.11126729739046873No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT105890.10890187744409587No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC104400.10736949669622824No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG102120.10502464561895429No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG97410.10018067694616468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA145700.0190.2084544
AATCGCA7150.0112.2826244
ACTTCTA29250.098.076844
ATGTTCT25900.096.7104644
ACATTGG13400.091.0662444
CGTGCGC37350.090.2770444
TTAAGCC140300.086.8352443
CACATGC85600.080.5321844
TCGTGCG72650.078.96001443
CTATCAT39800.076.6512344
CATGACC20450.068.5701844
AATCCCG31900.065.4705843
CCTTAGC146550.064.66872443
ACACATG49750.062.7836243
ATTAAGC189050.062.1290842
GAACACA41200.061.0559544
ACGACAC6500.054.34479544
ACCTTAG125600.054.18370442
TGTGTTA61050.053.69804442
TACACAT56150.051.8201942
Produced by FastQC (version 0.11.5)