##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841213.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9723432
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.71162568936565	37.0	35.0	39.0	30.0	39.0
2	36.52650720445209	38.0	35.0	39.0	32.0	39.0
3	36.77568311271164	38.0	35.0	39.0	32.0	39.0
4	37.24675382107881	39.0	36.0	40.0	33.0	41.0
5	37.67549852768035	39.0	36.0	40.0	33.0	41.0
6	37.68924614271998	39.0	36.0	40.0	33.0	41.0
7	37.66699607710529	39.0	36.0	40.0	33.0	41.0
8	37.484812461279105	39.0	36.0	40.0	33.0	41.0
9	37.42462620194186	39.0	36.0	40.0	32.0	41.0
10	37.25505994179833	38.0	35.0	40.0	32.0	41.0
11	37.112154638403396	38.0	35.0	40.0	32.0	41.0
12	37.020385189097844	38.0	35.0	40.0	31.0	41.0
13	36.8668944257542	38.0	35.0	40.0	31.0	41.0
14	36.83969147930484	38.0	35.0	40.0	31.0	41.0
15	36.80035382568624	38.0	35.0	40.0	31.0	41.0
16	36.73219054753507	38.0	35.0	40.0	31.0	41.0
17	36.708537787892176	38.0	35.0	40.0	31.0	41.0
18	36.57041382096363	38.0	35.0	40.0	31.0	41.0
19	36.45191625755186	38.0	35.0	40.0	31.0	41.0
20	36.33713003803595	38.0	34.0	40.0	30.0	41.0
21	36.05891407478347	38.0	34.0	40.0	30.0	41.0
22	36.16496047897491	38.0	34.0	40.0	30.0	41.0
23	36.20863487295432	38.0	34.0	40.0	30.0	41.0
24	36.13023086910054	38.0	34.0	40.0	30.0	41.0
25	36.14065208921029	38.0	34.0	40.0	30.0	41.0
26	36.11196435767933	38.0	34.0	40.0	30.0	41.0
27	36.12368598884349	37.0	34.0	40.0	30.0	41.0
28	36.129243031259136	37.0	34.0	40.0	30.0	41.0
29	36.118629431257425	37.0	34.0	40.0	30.0	41.0
30	36.08533462595488	37.0	34.0	40.0	31.0	41.0
31	35.99576370334795	37.0	34.0	40.0	30.0	41.0
32	35.98576029672383	37.0	34.0	40.0	30.0	41.0
33	35.902305184904634	37.0	34.0	40.0	30.0	41.0
34	35.789064008661725	36.0	34.0	40.0	30.0	41.0
35	35.72199049898083	36.0	34.0	39.0	30.0	41.0
36	35.574458737660066	36.0	34.0	39.0	30.0	41.0
37	35.50010938094556	36.0	34.0	39.0	30.0	41.0
38	35.41670446833234	35.0	34.0	39.0	30.0	41.0
39	35.27779181543532	35.0	33.0	39.0	30.0	40.0
40	35.12244324795649	35.0	33.0	39.0	30.0	40.0
41	34.97010464282142	35.0	33.0	39.0	29.0	40.0
42	34.82042875855331	35.0	33.0	38.0	29.0	40.0
43	34.706018605828405	35.0	33.0	38.0	29.0	40.0
44	34.61717734106512	35.0	33.0	38.0	29.0	40.0
45	34.55701131635663	35.0	33.0	37.0	29.0	40.0
46	34.43922490052608	35.0	33.0	37.0	29.0	40.0
47	34.3910618768349	35.0	33.0	37.0	29.0	40.0
48	34.39159926766693	35.0	33.0	37.0	29.0	40.0
49	34.48185945462157	35.0	33.0	37.0	29.0	40.0
50	35.09976314268887	35.0	33.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	18.0
21	81.0
22	318.0
23	1046.0
24	2805.0
25	6659.0
26	14821.0
27	32255.0
28	66331.0
29	127184.0
30	228213.0
31	375475.0
32	564453.0
33	800759.0
34	1054373.0
35	1121520.0
36	1288849.0
37	1380698.0
38	1260946.0
39	1012772.0
40	383854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.609757131021226	17.189352483773217	33.200890385205554
2	26.92333324283031	23.47945663629879	29.00880059633265	20.58840952453825
3	28.032900317501063	19.322765871145087	24.93412819671079	27.71020561464306
4	19.813343683588265	20.42456819773101	28.878651077109403	30.883437041571334
5	22.848722549815744	20.585704718251748	28.09976971094157	28.465803020990943
6	27.613099983627183	19.034390326378585	24.466145286972747	28.886364403021485
7	32.32580841826219	19.190209794237262	21.062717361524204	27.421264425976343
8	26.696715727533242	16.911960715105533	29.581355636569473	26.809967920791756
9	27.03526902846649	19.86329518219493	25.38469955875662	27.716736230581958
10	31.491144279098158	18.733786588932798	24.950069070262433	24.825000061706607
11	31.29440304616724	19.584689850250406	23.249949194893325	25.87095790868903
12	29.574084541343016	17.582331012342145	23.942256191023912	28.90132825529093
13	26.698145263935615	18.589866211847834	24.523285605329477	30.188702918887078
14	26.314134762293804	19.227336602960765	20.861173297658688	33.59735533708674
15	25.190735123154045	21.36450380894318	24.24361069219181	29.20115037571096
16	29.093420923805503	22.607717110583998	22.975025690517505	25.323836275092994
17	27.047836607485916	21.707633683250936	18.119980681718143	33.12454902754501
18	26.495727023133398	20.893960075002322	21.45530508158025	31.15500782028403
19	27.70198835143805	20.392820148276865	24.970936187963265	26.93425531232182
20	29.11560444912866	17.712243989570762	25.18153055423229	27.990621007068285
21	28.82601534108533	22.511187407902888	23.284690014801356	25.378107236210422
22	28.113324595677742	21.858845724431454	22.84432081182858	27.183508868062223
23	30.24829093266657	21.67754142775925	23.274827242068437	24.799340397505738
24	31.223748980812537	21.68653002355547	19.1922769655817	27.897444030050295
25	27.499784403264606	23.941045981848312	21.809651460602943	26.74951815428414
26	29.178871246072124	23.012927297551748	23.665613707474318	24.14258774890181
27	27.424145298466545	23.676427393226763	21.67791487111525	27.221512437191436
28	28.1041961143576	20.616356906868667	24.767210127897695	26.512236850876036
29	32.16524044056535	20.230924970927564	24.54958729852339	23.0542472899837
30	27.41023888710062	22.67226344292933	23.43588360109453	26.48161406887552
31	26.733952360841734	22.327321440957665	26.84693051503288	24.091795683167724
32	25.76692879617265	19.809902721860468	26.770026971976563	27.65314150999032
33	27.006229719467783	18.858018381318054	28.263920005725808	25.871831893488356
34	24.117474084113823	20.478490462300428	30.81600255976061	24.58803289382514
35	28.434332434731648	18.754237060794974	23.72192513855274	29.089505365920644
36	25.82503551614268	20.35033637564941	24.525578742032693	29.299049366175222
37	24.264424728065528	23.305475953108946	25.382091743731568	27.04800757509396
38	29.00566451819077	23.346129362875637	21.36636703729065	26.281839081642943
39	26.076541656699536	24.725347225298496	24.801315956563982	24.396795161437986
40	26.38295385500867	22.70066204144344	22.510066385800602	28.406317717747292
41	26.677345797372205	23.099240321443123	23.468333140013975	26.7550807411707
42	25.069891118090194	24.959725162871184	25.388601226538782	24.58178249249984
43	23.656635863739233	23.262794298646128	25.924844661928777	27.15572517568587
44	22.419255551365588	23.0066868707553	25.61494632969259	28.959111248186524
45	24.336315699636497	22.758141548733203	28.19238086927724	24.71316188235306
46	23.846125315990328	23.21723542215007	24.96798940680034	27.96864985505926
47	29.01828067741506	22.93453569601805	23.138657081397714	24.90852654516918
48	24.298736166195535	22.097182486831365	24.192325505772967	29.411755841200133
49	26.706051900933026	21.076273103036776	20.836809916321414	31.380865079708787
50	21.13891717446301	31.03993095044905	21.78775827540738	26.033393599680554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4334.0
1	4282.5
2	4226.0
3	4125.5
4	3872.0
5	3288.5
6	2644.0
7	2556.0
8	2770.0
9	3003.5
10	3307.5
11	3538.0
12	3620.5
13	3694.0
14	3815.5
15	4085.5
16	4428.0
17	5083.5
18	5985.5
19	7194.5
20	8601.5
21	10515.0
22	12841.5
23	16490.5
24	20837.5
25	25003.5
26	30158.5
27	36703.0
28	43887.5
29	51373.0
30	61082.5
31	81991.0
32	108158.0
33	122044.0
34	142563.0
35	174893.5
36	190924.0
37	209106.0
38	231718.5
39	243491.0
40	267226.5
41	322873.5
42	394874.0
43	518324.5
44	613613.5
45	547955.5
46	496630.5
47	515646.5
48	564913.5
49	648569.0
50	742187.0
51	819115.0
52	820398.0
53	811452.0
54	798132.5
55	774203.0
56	761386.0
57	768202.5
58	773155.5
59	730457.5
60	686790.5
61	665252.0
62	645487.5
63	614034.0
64	557519.5
65	500902.5
66	445557.0
67	399353.0
68	363387.5
69	323772.5
70	292265.5
71	270163.0
72	249113.0
73	221033.0
74	185821.0
75	155709.0
76	136094.0
77	110615.0
78	88148.5
79	71830.0
80	55444.0
81	42185.5
82	32792.0
83	25506.5
84	19446.5
85	14647.0
86	10785.5
87	7507.0
88	5095.5
89	3656.5
90	2460.5
91	1519.5
92	1119.0
93	949.0
94	602.5
95	236.5
96	151.0
97	147.0
98	85.0
99	21.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	98982.0
25	93533.0
26	86743.0
27	82942.0
28	77524.0
29	72453.0
30	99759.0
31	73597.0
32	81958.0
33	80111.0
34	78458.0
35	88656.0
36	78328.0
37	81382.0
38	83681.0
39	97873.0
40	99613.0
41	98346.0
42	109653.0
43	109634.0
44	111464.0
45	154567.0
46	548225.0
47	1163804.0
48	1815766.0
49	2358251.0
50	1798129.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.330927286854035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.79398424751986	10.978803798195399
2	12.860726591358645	4.972195411873282
3	7.2181423219142005	4.186001531132615
4	4.71714077734294	3.64746821474682
5	3.3463842838029887	3.234435563203334
6	2.5798463357519745	2.9922493156606946
7	1.9762735077307056	2.6742239633814764
8	1.5658283625045093	2.4215131375414716
9	1.274375363335765	2.217137173632168
>10	6.931985106429031	23.36160889428835
>50	0.4174858379920403	5.591615736256738
>100	0.2655332439716677	10.06041336648561
>500	0.027493355208946284	3.649234423452319
>1k	0.02044766156053431	7.355673498922358
>5k	0.002291054513733423	2.9963519429554855
>10k+	0.0020619490623600806	9.661074028271841
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	118234	1.2159698345193344	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	92099	0.9471861375695331	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	49453	0.50859614177381	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	43546	0.44784598689022564	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	40682	0.41839136634060897	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	35988	0.370116230565504	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	29800	0.3064761495735251	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	28601	0.29414511254873793	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	28068	0.2886635089338826	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	27963	0.2875836433061907	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	26362	0.2711182635925258	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	24926	0.25634981557951964	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	20421	0.21001843793426025	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	19843	0.2040740347646798	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	18631	0.19160930009074983	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	17621	0.18122202119580821	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	16853	0.17332357546183283	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	15739	0.1618667153737487	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	14563	0.14977222034359886	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	14469	0.14880548349595082	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	14445	0.14855865706676408	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	13536	0.13921010606131662	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	12960	0.13328627176083505	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	12440	0.12793836579512255	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	12412	0.12765040162773802	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	12328	0.12678650912558445	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	11900	0.12238477113842108	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	11811	0.12146945646352028	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	11655	0.11986508467380654	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	11182	0.11500054713191803	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	11119	0.11435262775530287	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	10854	0.11162725259969937	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	10819	0.11126729739046873	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	10589	0.10890187744409587	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	10440	0.10736949669622824	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	10212	0.10502464561895429	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	9741	0.10018067694616468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.16471550374394556	0.0	0.0	0.0	0.0
2	0.3692009158906032	0.0	0.0	0.0	0.0
3	0.5135840925302918	0.0	0.0	0.0	0.0
4	0.6315979789851979	0.0	0.0	0.0	0.0
5	0.7755286405047107	0.0	0.0	0.0	0.0
6	0.9285918799041326	0.0	0.0	0.0	0.0
7	1.0727796522873816	0.0	0.0	0.0	0.0
8	1.1866283427497617	0.0	0.0	0.0	0.0
9	1.2901308920553978	0.0	0.0	0.0	0.0
10	1.377353181469259	0.0	0.0	0.0	0.0
11	1.4537048235643546	0.0	0.0	0.0	0.0
12	1.5188875697387507	0.0	0.0	5.142217274723575E-5	0.0
13	1.567841478194119	0.0	0.0	5.142217274723575E-5	0.0
14	1.614255131315774	0.0	0.0	5.142217274723575E-5	0.0
15	1.6582827956219572	0.0	0.0	5.142217274723575E-5	0.0
16	1.6945045741051101	0.0	0.0	5.142217274723575E-5	0.0
17	1.7277027288307256	0.0	0.0	5.142217274723575E-5	0.0
18	1.7638628007065817	0.0	0.0	5.142217274723575E-5	0.0
19	1.8086206598657757	0.0	0.0	5.142217274723575E-5	0.0
20	1.829600906346648	0.0	0.0	5.142217274723575E-5	0.0
21	1.8585207362996934	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
22	1.8763539458084346	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
23	1.8914823490306716	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
24	1.9061582371327326	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
25	1.9222842305062657	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
26	1.9572410235398365	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
27	1.971649516343612	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
28	1.9853175298598273	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
29	1.9985947348631636	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
30	2.011285727097181	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
31	2.0236167641219684	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
32	2.0355775615029756	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
33	2.047250394716598	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
34	2.058265024119056	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
35	2.0696190398616454	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
36	2.080715944740499	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
37	2.0901467712223423	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
38	2.0970784801086695	0.0	0.0	5.142217274723575E-5	1.028443454944715E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	14570	0.0	190.20845	44
AATCGCA	715	0.0	112.28262	44
ACTTCTA	2925	0.0	98.0768	44
ATGTTCT	2590	0.0	96.71046	44
ACATTGG	1340	0.0	91.06624	44
CGTGCGC	3735	0.0	90.27704	44
TTAAGCC	14030	0.0	86.83524	43
CACATGC	8560	0.0	80.53218	44
TCGTGCG	7265	0.0	78.960014	43
CTATCAT	3980	0.0	76.65123	44
CATGACC	2045	0.0	68.57018	44
AATCCCG	3190	0.0	65.47058	43
CCTTAGC	14655	0.0	64.668724	43
ACACATG	4975	0.0	62.78362	43
ATTAAGC	18905	0.0	62.12908	42
GAACACA	4120	0.0	61.05595	44
ACGACAC	650	0.0	54.344795	44
ACCTTAG	12560	0.0	54.183704	42
TGTGTTA	6105	0.0	53.698044	42
TACACAT	5615	0.0	51.82019	42
>>END_MODULE