FastQCFastQC Report
Tue 24 May 2016
ERR841212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14216995
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG525370.36953660038566516No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA462390.3252375062381326No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG370670.2607231696993633Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT363900.2559612632627359No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC319060.22442154618468954No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA273560.19241759598283603No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG260390.18315403501232153No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG225210.15840900274636097Illumina Single End Adapter 1 (100% over 31bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG223420.1571499462439144No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA213380.15008797569387905Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA205630.14463675340675017No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC188170.13235567713148946No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA175570.12349304476789925Illumina Single End Adapter 1 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG174850.12298660863283696No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC169170.11899139023401217No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT164470.11568548768568884No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC161190.11337838973707172No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC155340.10926359613969057No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA150030.10552862964360611No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG146130.10278543391201866No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC145750.10251814817406914No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA144980.10197654286296086Illumina Single End Adapter 1 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA72750.0108.9850244
TTAAGCC61000.071.2833243
ATTAGTC18200.067.99802444
CTATCAT43500.064.67576644
TCGTGCG110200.060.74729543
CGTGCGC41600.060.2915844
AATCGCA6350.058.46759444
ACTTCTA32000.056.2060344
ATTAAGC78000.054.7658642
AACGACA19250.051.10106743
CGCTACC19150.048.68398344
TACGCTA34750.045.3127642
CTCGTGC127150.042.47324842
AATCCCG17900.041.824243
ACCTTAG55900.041.22081442
ATGTTCT34300.041.13186644
GATTAAG96650.041.1067941
CTAATCC76000.040.79987341
TGTGTTA51600.040.30510742
AGATTAA89400.040.15256540
Produced by FastQC (version 0.11.5)