##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841212.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14216995
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.88843725414548	37.0	35.0	39.0	30.0	39.0
2	36.74115127704554	38.0	35.0	39.0	32.0	39.0
3	37.026242817135405	38.0	37.0	39.0	33.0	39.0
4	37.52445759459014	39.0	37.0	40.0	33.0	41.0
5	38.13556753730307	39.0	37.0	41.0	33.0	41.0
6	38.14520480593824	40.0	37.0	41.0	33.0	41.0
7	38.10600636773101	40.0	37.0	41.0	33.0	41.0
8	38.04433060572927	39.0	37.0	41.0	33.0	41.0
9	37.992706334918175	39.0	37.0	40.0	33.0	41.0
10	37.83947486793095	39.0	37.0	40.0	33.0	41.0
11	37.762417022725266	39.0	37.0	40.0	33.0	41.0
12	37.69510680702919	39.0	36.0	40.0	33.0	41.0
13	37.611596895124464	39.0	36.0	40.0	32.0	41.0
14	37.49754276483884	39.0	36.0	40.0	32.0	41.0
15	37.46314984284653	39.0	36.0	40.0	32.0	41.0
16	37.40538186867197	39.0	36.0	40.0	32.0	41.0
17	37.38334718412717	39.0	36.0	40.0	32.0	41.0
18	37.29872655930455	39.0	36.0	40.0	32.0	41.0
19	37.1834055649594	38.0	36.0	40.0	31.0	41.0
20	37.08436290510055	38.0	35.0	40.0	31.0	41.0
21	36.876794006046985	38.0	35.0	40.0	31.0	41.0
22	36.86581665112775	38.0	35.0	40.0	31.0	41.0
23	36.87859297974009	38.0	35.0	40.0	31.0	41.0
24	36.88521125596513	38.0	35.0	40.0	31.0	41.0
25	36.88611232868945	38.0	35.0	40.0	31.0	41.0
26	36.91718700942837	38.0	35.0	40.0	31.0	41.0
27	36.95537905704845	38.0	35.0	40.0	31.0	41.0
28	36.940846289600486	38.0	35.0	40.0	31.0	41.0
29	36.966797426620545	38.0	35.0	40.0	31.0	41.0
30	37.0114950535764	38.0	35.0	40.0	31.0	41.0
31	36.97729250027876	38.0	35.0	40.0	31.0	41.0
32	36.907973216465294	38.0	35.0	40.0	31.0	41.0
33	36.846076392172776	38.0	35.0	40.0	31.0	41.0
34	36.81713237008382	38.0	35.0	40.0	31.0	41.0
35	36.72943561554561	38.0	35.0	40.0	31.0	41.0
36	36.639191161361964	38.0	35.0	40.0	31.0	41.0
37	36.5784809276856	38.0	35.0	40.0	31.0	41.0
38	36.49275533288219	38.0	35.0	40.0	31.0	41.0
39	36.36641845691747	38.0	34.0	40.0	31.0	41.0
40	36.240069500730485	37.0	34.0	40.0	31.0	41.0
41	36.09444130034959	37.0	34.0	40.0	30.0	41.0
42	35.95881113111646	37.0	34.0	40.0	30.0	41.0
43	35.8354043593748	37.0	34.0	40.0	30.0	41.0
44	35.73648660538708	36.0	34.0	40.0	30.0	41.0
45	35.64526027355799	36.0	34.0	39.0	30.0	41.0
46	35.56776363803718	36.0	34.0	39.0	30.0	41.0
47	35.538166964852124	36.0	34.0	39.0	30.0	41.0
48	35.54871439894145	36.0	34.0	39.0	30.0	40.0
49	35.58353750243511	36.0	34.0	39.0	30.0	40.0
50	36.0257674505369	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	18.0
21	55.0
22	139.0
23	399.0
24	1070.0
25	2604.0
26	6908.0
27	17464.0
28	43104.0
29	99130.0
30	201760.0
31	364981.0
32	591718.0
33	876546.0
34	1211418.0
35	1434065.0
36	1767361.0
37	2061432.0
38	2230022.0
39	2186094.0
40	1120707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.58058049538598	32.11645639602462	25.302963108589406
2	30.06656469950225	26.429959354983247	23.595204190477663	19.90827175503684
3	26.178408306396676	20.035225446727665	24.93806883944181	28.848297407433847
4	21.72504808505595	21.498741471035192	29.7744213879234	27.00178905598546
5	24.075214206659002	22.98283849716484	25.640945924226603	27.301001371949557
6	26.149850935447326	23.53531108367134	25.18907828271727	25.125759698164064
7	31.155606371107258	21.706359184905107	22.43613365552988	24.70190078845776
8	29.025993186323834	20.25389331571123	24.977584925647086	25.742528572317845
9	28.18011119790082	22.219688478472417	25.170593363787496	24.429606959839262
10	27.079280818485202	20.642301695963177	26.759930632317168	25.518486853234457
11	28.484655160953494	22.263741388387633	25.028390317363126	24.223213133295747
12	28.40883745123354	22.124780939994704	24.94342862187122	24.52295298690054
13	27.939736913461672	20.28996985649921	23.765465205551525	28.004828024487594
14	26.603005768799946	22.85791758384947	23.29489459622093	27.244182051129652
15	25.8215537108932	23.715609381588727	24.21274678650446	26.25009012101362
16	27.28228433645788	23.729226886553732	23.786496372827028	25.201992404161356
17	28.570186597097347	22.258487113486357	21.201372019895906	27.969954269520386
18	28.455858639607033	21.875881647282004	21.69661732314037	27.971642389970597
19	26.544132568098956	22.03097771364483	24.318824055294385	27.106065662961825
20	29.81715193681928	19.92206510588208	23.921159147907133	26.339623809391504
21	29.904673948327336	23.005860239804544	22.82686320140086	24.26260261046726
22	28.830325958474347	21.43146283725921	23.58006737710747	26.158143827158973
23	28.81736260018379	22.232736242785485	24.224809813888236	24.725091343142484
24	30.280048631936634	21.42600458113687	22.917972468865607	25.375974318060884
25	26.933168413738624	23.886982803451687	24.116478063801516	25.063370719008176
26	27.97773373445057	23.186746870038718	23.342152434826993	25.493366960683716
27	28.114499700207098	22.995630783289325	23.899500422299038	24.990369094204542
28	27.617489537428053	22.114089110312804	24.34094920014422	25.927472152114923
29	28.978967968228865	21.909861038098562	24.35043373135603	24.760737262316542
30	26.8606332695255	23.65149485475651	24.868272552684857	24.61959932303313
31	27.44742771661816	22.198505877783706	26.129455669859308	24.224610735738825
32	26.739039995842827	22.642316607169185	25.778782057944184	24.839861339043804
33	25.35418461911199	21.959267003613505	27.230291964869046	25.456256412405466
34	25.062985125644072	22.52742898148367	28.04873925386454	24.360846639007715
35	26.858460273689943	21.95447599118828	25.347531637409805	25.83953209771197
36	25.734502850978313	23.27036273273216	24.870059563271845	26.125074853017676
37	24.80028514483939	24.614007867191823	25.938206345647025	24.64750064232176
38	26.59693947716068	24.304448484308377	23.22129870263093	25.87731333590001
39	25.992930175862178	25.058834828467813	24.837265583621154	24.110969412048853
40	25.61884363878861	24.079506051311416	24.576078214343116	25.725572095556853
41	25.824777398776895	25.034478084180336	23.87393699677445	25.266807520268326
42	25.093329353797607	25.093902353012854	24.854927974419965	24.957840318769577
43	23.692568677294904	25.124017168426477	26.01362009814729	25.169794056131334
44	23.579888086454517	24.15280548002062	24.960477619848145	27.306828813676713
45	25.132248201386627	24.078133934611763	26.038433122658883	24.751184741342726
46	24.518097486744235	24.835334196900245	25.097603884133136	25.54896443222238
47	27.884041101202527	23.934801788980153	23.732017371618873	24.44913973819844
48	23.813687816560336	25.47117746242091	24.29881696131862	26.41631775970013
49	25.85394797542523	24.31867121691145	23.267476039046592	26.559904768616725
50	23.311550524069908	27.543662776462906	24.326695503142087	24.8180911963251
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21545.0
1	21218.0
2	16556.0
3	11990.0
4	11365.5
5	9621.5
6	7329.0
7	6647.0
8	6963.0
9	7177.0
10	7346.0
11	7495.0
12	7730.5
13	8165.0
14	8910.5
15	10123.0
16	11511.5
17	13584.5
18	16174.0
19	19932.5
20	24205.0
21	29919.0
22	36608.5
23	45690.5
24	55911.0
25	66093.5
26	77984.0
27	93037.5
28	109802.5
29	126810.0
30	146549.0
31	175022.0
32	207706.5
33	232456.5
34	265123.0
35	312713.5
36	348297.5
37	376711.0
38	411226.5
39	462561.0
40	527661.5
41	612953.0
42	723339.0
43	842277.5
44	909345.5
45	880715.0
46	867143.0
47	897042.5
48	961868.5
49	1054681.0
50	1181554.0
51	1294553.5
52	1347785.5
53	1350497.5
54	1266883.0
55	1128555.5
56	1003749.5
57	941731.0
58	915672.5
59	888964.5
60	867679.0
61	838786.5
62	796459.0
63	743366.0
64	661666.5
65	585879.5
66	526701.0
67	482279.0
68	431406.0
69	369686.0
70	318226.0
71	280413.5
72	258776.5
73	236873.0
74	200154.0
75	164509.0
76	140033.5
77	109473.5
78	85488.5
79	71507.5
80	56103.5
81	41559.5
82	31017.5
83	23860.0
84	18122.0
85	13633.0
86	10082.5
87	6962.5
88	4647.5
89	3354.5
90	2481.0
91	1750.0
92	1276.5
93	1053.5
94	673.5
95	268.0
96	158.0
97	145.0
98	85.5
99	25.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	143066.0
25	126552.0
26	119498.0
27	126659.0
28	119080.0
29	102603.0
30	116507.0
31	123254.0
32	139193.0
33	116962.0
34	131194.0
35	130829.0
36	118880.0
37	124850.0
38	139513.0
39	156037.0
40	158013.0
41	156924.0
42	164969.0
43	166543.0
44	170308.0
45	207962.0
46	597616.0
47	1494768.0
48	2566517.0
49	3067858.0
50	3430840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.09691496659457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.24311662551155	12.076531638712732
2	12.068365263792995	5.092105515120889
3	6.76688005908046	4.282808795866947
4	4.602702557981416	3.8841129792904496
5	3.287470235667108	3.46777400085398
6	2.6123501363812482	3.3067517214124154
7	2.095224191937245	3.094193662927632
8	1.6166488776827237	2.7285043122650374
9	1.3483660093678742	2.5601726739132453
>10	7.761391715915932	27.919169081282107
>50	0.3517175718972267	5.108045696483429
>100	0.2052786195704461	8.338502624674945
>500	0.02059802155289072	2.99693156074175
>1k	0.016857231510180135	7.400840528679943
>5k	0.0016927714330018993	2.4697851538801636
>10k+	0.0013401107177931704	5.273770053894305
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	52537	0.36953660038566516	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	46239	0.3252375062381326	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	37067	0.2607231696993633	Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	36390	0.2559612632627359	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	31906	0.22442154618468954	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	27356	0.19241759598283603	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	26039	0.18315403501232153	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	22521	0.15840900274636097	Illumina Single End Adapter 1 (100% over 31bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	22342	0.1571499462439144	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	21338	0.15008797569387905	Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	20563	0.14463675340675017	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	18817	0.13235567713148946	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	17557	0.12349304476789925	Illumina Single End Adapter 1 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	17485	0.12298660863283696	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	16917	0.11899139023401217	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	16447	0.11568548768568884	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	16119	0.11337838973707172	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	15534	0.10926359613969057	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	15003	0.10552862964360611	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	14613	0.10278543391201866	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	14575	0.10251814817406914	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	14498	0.10197654286296086	Illumina Single End Adapter 1 (100% over 32bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.3578322985975588	0.0	0.0	0.0	0.0
2	0.8141945608055711	0.0	0.0	0.0	0.0
3	1.049469314718054	0.0	0.0	0.0	0.0
4	1.2281498305373253	0.0	0.0	0.0	0.0
5	1.5006757757177238	0.0	0.0	0.0	0.0
6	1.7263141753936047	0.0	0.0	0.0	0.0
7	1.9477744769552217	0.0	0.0	0.0	0.0
8	2.127313120670015	0.0	0.0	0.0	0.0
9	2.280622592889707	0.0	0.0	0.0	0.0
10	2.4175150937311294	0.0	0.0	0.0	0.0
11	2.552986759860294	0.0	0.0	0.0	0.0
12	2.672758905802527	0.0	0.0	0.0	0.0
13	2.758642033706842	0.0	0.0	0.0	0.0
14	2.8510736621909203	0.0	0.0	0.0	0.0
15	2.9561099233698824	0.0	0.0	0.0	0.0
16	3.030345020167764	6.330451688278712E-5	0.0	0.0	0.0
17	3.1068379780677984	6.330451688278712E-5	0.0	0.0	0.0
18	3.1860741316994203	6.330451688278712E-5	0.0	0.0	0.0
19	3.3592963913963536	6.330451688278712E-5	0.0	0.0	0.0
20	3.4139633586422446	6.330451688278712E-5	0.0	0.0	0.0
21	3.5030750169075815	6.330451688278712E-5	0.0	0.0	0.0
22	3.536999204121546	6.330451688278712E-5	0.0	0.0	0.0
23	3.5693759475894873	6.330451688278712E-5	0.0	0.0	0.0
24	3.595647322095844	6.330451688278712E-5	0.0	0.0	0.0
25	3.637203220511789	6.330451688278712E-5	0.0	0.0	0.0
26	3.769376017927839	6.330451688278712E-5	0.0	0.0	0.0
27	3.7968642459253874	6.330451688278712E-5	0.0	0.0	0.0
28	3.819555398310262	6.330451688278712E-5	0.0	0.0	0.0
29	3.83993241891131	6.330451688278712E-5	0.0	0.0	0.0
30	3.8602179996546386	6.330451688278712E-5	0.0	0.0	0.0
31	3.879532911139098	6.330451688278712E-5	0.0	0.0	0.0
32	3.8980529992449178	6.330451688278712E-5	0.0	0.0	0.0
33	3.91506081278076	6.330451688278712E-5	0.0	0.0	0.0
34	3.9333698858303037	6.330451688278712E-5	0.0	0.0	0.0
35	3.951981413793843	6.330451688278712E-5	0.0	0.0	0.0
36	3.96780754301454	6.330451688278712E-5	0.0	7.033835209198569E-6	0.0
37	3.983289014309986	6.330451688278712E-5	0.0	1.4067670418397137E-5	0.0
38	3.9933192633183032	6.330451688278712E-5	0.0	1.4771053939316995E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	7275	0.0	108.98502	44
TTAAGCC	6100	0.0	71.28332	43
ATTAGTC	1820	0.0	67.998024	44
CTATCAT	4350	0.0	64.675766	44
TCGTGCG	11020	0.0	60.747295	43
CGTGCGC	4160	0.0	60.29158	44
AATCGCA	635	0.0	58.467594	44
ACTTCTA	3200	0.0	56.20603	44
ATTAAGC	7800	0.0	54.76586	42
AACGACA	1925	0.0	51.101067	43
CGCTACC	1915	0.0	48.683983	44
TACGCTA	3475	0.0	45.31276	42
CTCGTGC	12715	0.0	42.473248	42
AATCCCG	1790	0.0	41.8242	43
ACCTTAG	5590	0.0	41.220814	42
ATGTTCT	3430	0.0	41.131866	44
GATTAAG	9665	0.0	41.10679	41
CTAATCC	7600	0.0	40.799873	41
TGTGTTA	5160	0.0	40.305107	42
AGATTAA	8940	0.0	40.152565	40
>>END_MODULE