FastQCFastQC Report
Tue 24 May 2016
ERR841211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4495710
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG86850.193184168907692No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC75610.16818255625918932No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA74520.165758022648258No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC73680.16388957472790727No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG63180.14053397572352308No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA57560.12803316939927176No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT57290.12743259685344474No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA55740.12398486557184515No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG55030.12240558221059633No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT54810.12191622680288541No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT52640.11708940300864602No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT51780.11517646823304883No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG49840.11086124327414357No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG48190.10719107771631177No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG48110.107013130295326Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA47560.10578974177604872No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG46480.10338745159274064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA15450.0108.48492444
CTATACG1900.096.8217544
ATGTTCT13250.088.7025344
GACCGTA1450.077.5315944
CGCTACC4850.071.6458844
TTAAGCC10750.068.4924443
CTATCAT6250.058.86762644
TTCAGGA14250.058.8102544
AGGTCCC19350.057.75651643
CACATGC13800.056.28446644
ATTAAGC13900.056.20147742
ATTAGTC3700.055.2436444
AATCCCG8350.054.58692643
GGTCCCA15650.051.59014544
TGTGTTA15100.049.84238442
CAATGAA16700.049.33818443
TACGCTA6950.048.43379642
AATGAAG17150.047.07789244
ATCCTTG12300.046.3212243
AACGACA5000.045.58008643
Produced by FastQC (version 0.11.5)