##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841211.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4495710
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.21631978041288	38.0	35.0	39.0	31.0	39.0
2	36.91635470259425	38.0	37.0	39.0	33.0	39.0
3	36.995999964410515	39.0	36.0	39.0	33.0	39.0
4	37.577270108614655	39.0	37.0	40.0	33.0	41.0
5	38.12248877262991	39.0	37.0	41.0	33.0	41.0
6	38.13611887777459	40.0	37.0	41.0	33.0	41.0
7	38.16258699960629	40.0	37.0	41.0	33.0	41.0
8	37.98997355256456	39.0	37.0	41.0	33.0	41.0
9	38.041698641593875	39.0	37.0	41.0	33.0	41.0
10	37.909144273095905	39.0	37.0	40.0	33.0	41.0
11	37.8242070774138	39.0	37.0	40.0	33.0	41.0
12	37.77894259193765	39.0	37.0	40.0	33.0	41.0
13	37.61104764319763	39.0	36.0	40.0	33.0	41.0
14	37.53464814234014	39.0	36.0	40.0	32.0	41.0
15	37.312206525776794	39.0	36.0	40.0	32.0	41.0
16	37.391202279506466	39.0	36.0	40.0	32.0	41.0
17	37.23199694820173	38.0	36.0	40.0	32.0	41.0
18	37.217355211968744	38.0	36.0	40.0	32.0	41.0
19	37.153873804137724	38.0	35.0	40.0	31.0	41.0
20	37.036227648135664	38.0	35.0	40.0	31.0	41.0
21	36.85993980928485	38.0	35.0	40.0	31.0	41.0
22	36.845192861639205	38.0	35.0	40.0	31.0	41.0
23	36.819420291789285	38.0	35.0	40.0	31.0	41.0
24	36.7729141781832	38.0	35.0	40.0	31.0	41.0
25	36.76296755115089	38.0	35.0	40.0	31.0	41.0
26	36.76608741824423	38.0	35.0	40.0	31.0	41.0
27	36.78834902252189	38.0	35.0	40.0	31.0	41.0
28	36.77375586022458	38.0	35.0	40.0	31.0	41.0
29	36.77846835736009	38.0	35.0	40.0	31.0	41.0
30	36.77669147262782	38.0	35.0	40.0	31.0	41.0
31	36.678397770936286	38.0	35.0	40.0	31.0	41.0
32	36.64662014575203	38.0	35.0	40.0	31.0	41.0
33	36.56072484398027	38.0	35.0	40.0	31.0	41.0
34	36.48939363738899	38.0	35.0	40.0	31.0	41.0
35	36.38333575048035	37.0	34.0	40.0	31.0	41.0
36	36.26265207293462	37.0	34.0	40.0	31.0	41.0
37	36.186713530407886	37.0	34.0	40.0	31.0	41.0
38	36.105436056337744	37.0	34.0	40.0	31.0	41.0
39	35.95791405926174	37.0	34.0	40.0	31.0	41.0
40	35.80059086904058	36.0	34.0	40.0	30.0	41.0
41	35.62607128746126	36.0	34.0	39.0	30.0	41.0
42	35.481556680942404	36.0	34.0	39.0	30.0	41.0
43	35.33205613921056	35.0	33.0	39.0	30.0	40.0
44	35.202029467515416	35.0	33.0	39.0	30.0	40.0
45	35.13268741137976	35.0	33.0	39.0	30.0	40.0
46	35.034655750432684	35.0	33.0	39.0	30.0	40.0
47	34.9925669112965	35.0	33.0	38.0	30.0	40.0
48	34.99613549882942	35.0	33.0	38.0	30.0	40.0
49	34.96705626393641	35.0	33.0	38.0	30.0	40.0
50	35.44196455594425	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	18.0
22	67.0
23	174.0
24	501.0
25	1200.0
26	2782.0
27	6859.0
28	15870.0
29	34741.0
30	69481.0
31	122936.0
32	197520.0
33	292234.0
34	406567.0
35	470513.0
36	584299.0
37	675450.0
38	694646.0
39	620348.0
40	299501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.85759535201336	19.282493755157695	29.85991089282894
2	34.48376341000643	20.635361266629744	26.66993645052728	18.21093887283655
3	26.23211906461938	15.506071343569758	29.411283201096154	28.850526390714705
4	24.46229850235002	18.14024925984995	31.52213999568477	25.87531224211526
5	24.794593067613345	16.9748271129588	25.52947142942939	32.70110838999847
6	23.64939019643171	22.575566484492995	28.792515531473338	24.982527787601956
7	37.22152896872796	16.831601682492867	21.69036703879921	24.256502309979957
8	24.430512644276433	15.470148207958253	31.75369407724253	28.345645070522785
9	31.924012002553546	16.947534427265104	25.67345313643451	25.45500043374684
10	26.67218303671723	16.074835787895573	26.274559524524495	30.978421650862707
11	29.813155207964925	17.082040434102733	30.03921516289974	23.065589195032597
12	32.060008319041934	16.439850435192664	21.27490429765265	30.225236948112755
13	26.946021874186727	16.65730218363729	30.498964568444137	25.897711373731845
14	34.212371349575484	18.480462485347143	20.74893620807392	26.558229957003455
15	25.2101447824704	18.42854632527454	29.479259115912726	26.88204977634234
16	33.712984156006506	18.77216279519809	22.91813751331825	24.596715535477152
17	29.880285872531815	18.399385191660496	23.730000378138268	27.990328557669425
18	33.40397845946469	18.484266111470713	20.54460808192699	27.567147347137606
19	32.93633263711405	18.163026529736126	23.175916596043784	25.724724237106038
20	33.5803243536616	17.877843544178784	22.784321052736942	25.75751104942267
21	32.49366618398429	21.15363313025084	21.186175264863614	25.16652542090126
22	31.144424351214823	21.10104966734954	22.946942752090326	24.80758322934531
23	30.82901254751752	21.03024883722482	22.712986380349268	25.42775223490839
24	31.712054380731853	21.122425601295458	21.526544194354173	25.638975823618516
25	30.25257550504325	21.723504985775453	23.546069930170408	24.47784957901089
26	30.13573410651997	21.87600408028793	22.776497813241917	25.211763999950186
27	29.908627170318724	21.55483340010798	23.425824781041936	25.110714648531356
28	30.80308954952508	20.61282356379906	23.11472806486581	25.469358821810044
29	31.67849335737169	20.665315707474818	23.69873548178826	23.957455453365238
30	29.87679062485989	21.551786141292165	23.07022107725187	25.501202156596076
31	28.043908484571656	20.960628841715604	26.552864056182656	24.442598617530084
32	28.519042027352477	20.25233213692755	25.22709085437802	26.001534981341955
33	28.027062807859664	19.983977354789094	25.89785096929344	26.0911088680578
34	27.41751853633982	19.313628161240455	28.044325054258884	25.224528248160844
35	28.63946905824708	19.906373676003668	24.78406279261268	26.670094473136572
36	27.92761034961279	20.82785806534285	24.45944283967507	26.78508874536929
37	26.524453477729494	22.187321643633275	25.63819713362601	25.650027745011215
38	29.09071028320158	22.182026702961632	23.065992493692736	25.661270520144054
39	27.847046802892155	22.37611388212409	23.985219210994124	25.791620103989626
40	28.427077371086185	21.765077336952523	22.89643005158514	26.91141524037615
41	27.77725978099554	21.873246931470057	23.7562453670053	26.593247920529105
42	27.44654412454281	22.67782154282201	24.097319912053102	25.778314420582078
43	25.588823861169306	23.70711463514103	24.281706904656513	26.42235459903315
44	25.462157689438804	23.382454770759196	24.607086426816455	26.548301112985545
45	26.718334640302484	21.928159434153287	25.30875960269945	26.04474632284478
46	26.00119356073158	22.739125312564752	24.096517843125948	27.163163283577724
47	27.771922051017206	22.071264455510335	24.209667622297797	25.947145871174666
48	25.344295294663354	23.204458925676448	24.55539159560198	26.895854184058216
49	26.74951526056849	22.28820437287919	22.958225782915793	28.004054583636524
50	24.934757308609065	25.46069620376049	24.319163248920425	25.28538323871002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6865.0
1	6774.0
2	5339.5
3	3902.5
4	3641.0
5	2912.0
6	1944.0
7	1553.0
8	1529.0
9	1523.5
10	1535.0
11	1552.5
12	1557.5
13	1529.5
14	1593.0
15	1799.0
16	2013.5
17	2310.0
18	2801.0
19	3644.0
20	4545.5
21	5471.5
22	6772.0
23	8976.0
24	11480.0
25	13544.5
26	15969.0
27	19108.0
28	22174.0
29	25287.5
30	28673.5
31	32904.5
32	39280.0
33	46564.5
34	54213.0
35	62597.0
36	69929.0
37	77560.0
38	86021.5
39	96794.0
40	110849.5
41	132372.5
42	161865.0
43	190486.5
44	208235.0
45	208636.0
46	214992.0
47	232601.5
48	259338.0
49	291649.0
50	324249.5
51	356119.0
52	386955.0
53	407149.0
54	398099.5
55	379160.5
56	369360.5
57	370055.0
58	371388.5
59	358861.0
60	343071.0
61	332163.5
62	316873.5
63	298569.0
64	276799.5
65	254242.5
66	228974.5
67	206207.5
68	183898.5
69	159499.0
70	138388.0
71	121245.5
72	109546.0
73	98335.0
74	83049.0
75	68698.0
76	58490.0
77	46512.0
78	36912.0
79	30356.0
80	23396.0
81	16908.5
82	12169.0
83	9132.0
84	6944.5
85	5247.0
86	3736.5
87	2530.5
88	1733.5
89	1214.0
90	821.0
91	545.5
92	400.5
93	336.0
94	230.0
95	112.5
96	78.0
97	71.0
98	41.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	41438.0
25	37917.0
26	35950.0
27	35435.0
28	32972.0
29	29694.0
30	37293.0
31	31279.0
32	35895.0
33	33815.0
34	34494.0
35	35907.0
36	33255.0
37	34668.0
38	37033.0
39	42922.0
40	43265.0
41	43798.0
42	47818.0
43	50472.0
44	51193.0
45	64748.0
46	216579.0
47	577233.0
48	781598.0
49	1173462.0
50	875577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.41167982120728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.783181779817546	13.293896710584995
2	12.447173589166361	5.828184854971006
3	6.894666313626921	4.842471606260909
4	4.864388143871708	4.555339910016048
5	3.4908164213508903	4.086293818563982
6	2.7265931959486323	3.8300476143739
7	2.1302744144080834	3.491131176500232
8	1.6959779804580657	3.1764554769841573
9	1.3698038519849267	2.8862468280474762
>10	7.069348098368438	27.51721962665668
>50	0.2916387460824289	4.696966698814446
>100	0.19637278230429278	9.000434479593808
>500	0.023317510230129988	3.8388858087053612
>1k	0.015198020048801686	7.107450159637807
>5k	0.001249152332778221	1.8489752302892632
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	8685	0.193184168907692	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	7561	0.16818255625918932	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	7452	0.165758022648258	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	7368	0.16388957472790727	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	6318	0.14053397572352308	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	5756	0.12803316939927176	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	5729	0.12743259685344474	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5574	0.12398486557184515	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	5503	0.12240558221059633	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	5481	0.12191622680288541	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	5264	0.11708940300864602	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	5178	0.11517646823304883	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	4984	0.11086124327414357	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	4819	0.10719107771631177	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	4811	0.107013130295326	Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	4756	0.10578974177604872	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	4648	0.10338745159274064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.21080096358528463	0.0	0.0	0.0	0.0
2	0.4604389518007167	0.0	0.0	0.0	0.0
3	0.6217705323519533	0.0	0.0	0.0	0.0
4	0.7625491857793318	0.0	0.0	0.0	0.0
5	0.9385391851342725	0.0	0.0	0.0	0.0
6	1.1189111397309879	0.0	0.0	0.0	0.0
7	1.2647835380840846	0.0	0.0	0.0	0.0
8	1.3727976226224556	0.0	0.0	0.0	0.0
9	1.4679327625669805	0.0	0.0	0.0	0.0
10	1.5413583171512397	0.0	0.0	0.0	0.0
11	1.6172751356293	0.0	0.0	0.0	0.0
12	1.6785557787312795	0.0	0.0	0.0	0.0
13	1.72680177324605	0.0	0.0	0.0	6.673028286966909E-5
14	1.7766715379773161	0.0	0.0	0.0	6.673028286966909E-5
15	1.834704640646305	0.0	0.0	0.0	6.673028286966909E-5
16	1.874186724677526	0.0	0.0	0.0	6.673028286966909E-5
17	1.9147142498070382	0.0	0.0	0.0	6.673028286966909E-5
18	1.9492582929059037	0.0	0.0	0.0	6.673028286966909E-5
19	2.035073436676298	0.0	0.0	0.0	6.673028286966909E-5
20	2.066392182769796	0.0	0.0	0.0	6.673028286966909E-5
21	2.113881900745377	0.0	0.0	0.0	6.673028286966909E-5
22	2.138616592262401	0.0	0.0	0.0	6.673028286966909E-5
23	2.1619721912667855	0.0	0.0	0.0	6.673028286966909E-5
24	2.1843045926005016	0.0	0.0	0.0	6.673028286966909E-5
25	2.2113526005903408	0.0	0.0	0.0	6.673028286966909E-5
26	2.2860237871215	0.0	0.0	0.0	6.673028286966909E-5
27	2.309957715244088	0.0	0.0	0.0	6.673028286966909E-5
28	2.3312001886242664	0.0	0.0	0.0	6.673028286966909E-5
29	2.3509523523536884	0.0	0.0	0.0	6.673028286966909E-5
30	2.369258693287601	0.0	0.0	0.0	6.673028286966909E-5
31	2.3878764422082384	0.0	0.0	0.0	6.673028286966909E-5
32	2.405515480313454	0.0	0.0	0.0	6.673028286966909E-5
33	2.4219978601822625	0.0	0.0	0.0	6.673028286966909E-5
34	2.438480240051071	0.0	0.0	0.0	6.673028286966909E-5
35	2.455807870169562	0.0	0.0	0.0	6.673028286966909E-5
36	2.4707554535323677	0.0	0.0	0.0	6.673028286966909E-5
37	2.48367888498146	0.0	0.0	0.0	6.673028286966909E-5
38	2.493421506280432	0.0	0.0	0.0	6.673028286966909E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	1545	0.0	108.484924	44
CTATACG	190	0.0	96.82175	44
ATGTTCT	1325	0.0	88.70253	44
GACCGTA	145	0.0	77.53159	44
CGCTACC	485	0.0	71.64588	44
TTAAGCC	1075	0.0	68.49244	43
CTATCAT	625	0.0	58.867626	44
TTCAGGA	1425	0.0	58.81025	44
AGGTCCC	1935	0.0	57.756516	43
CACATGC	1380	0.0	56.284466	44
ATTAAGC	1390	0.0	56.201477	42
ATTAGTC	370	0.0	55.24364	44
AATCCCG	835	0.0	54.586926	43
GGTCCCA	1565	0.0	51.590145	44
TGTGTTA	1510	0.0	49.842384	42
CAATGAA	1670	0.0	49.338184	43
TACGCTA	695	0.0	48.433796	42
AATGAAG	1715	0.0	47.077892	44
ATCCTTG	1230	0.0	46.32122	43
AACGACA	500	0.0	45.580086	43
>>END_MODULE