FastQCFastQC Report
Tue 24 May 2016
ERR841209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817247
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG31570.38629692124902265No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG20480.25059743259993617Illumina Single End Adapter 1 (100% over 32bp)
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC20150.24655948568792543No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT17110.20936142928637244No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA16540.2023867937110812No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG15700.19210838338959946No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA13550.16580054744771164Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGT13460.16469928919898147No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGAAGCAGTGGT13120.16053898025933408No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA12960.15858118781714708No Hit
AAGCAGTGGTATCAACGCAGAGTG12680.15515505104331984No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC10990.13447586837271963No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT10590.12958138726725213Illumina Single End Adapter 2 (100% over 32bp)
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC10350.12664469860397162No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA10060.12309619980250769Illumina Single End Adapter 1 (100% over 32bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC9950.12175021749850413No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA9900.1211384073603207Illumina Single End Adapter 1 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA9060.11085999703883892No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG8670.10608787796100813No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG8610.10535370579518799No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAC8180.10009213860681042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCA600.0127.36819544
TAATCGC600.077.200943
ACTTCTA2350.076.86436544
TCGTGCG1700.069.3569543
CTATCAT3750.059.28410344
CGCTACC851.8195351E-857.2135244
TAATTCC1103.8198777E-1156.84200344
CATGACC2100.056.240544
GGTGCGT502.477768E-455.57884644
AACGACA803.6379788E-1252.6369743
CTAATCG700.052.56562442
CCCTATC2750.051.29130642
TCGCAAA509.724827E-750.5314943
GTTCAGG4400.049.7658643
CTCGTGC2150.045.6383742
ATGTTCT1900.043.8780444
ACCTTAG3600.043.4396542
ACATTGG1603.092282E-1143.42097544
CAGGCAC4200.043.00744244
CCATGAC1700.042.10957743
Produced by FastQC (version 0.11.5)