##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841209.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 817247 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.377641031414 38.0 35.0 39.0 32.0 39.0 2 37.006299197182734 39.0 37.0 39.0 33.0 39.0 3 37.116051817871465 39.0 37.0 39.0 33.0 39.0 4 37.482280142967795 39.0 37.0 39.0 33.0 41.0 5 38.210762474502815 40.0 37.0 41.0 33.0 41.0 6 38.23004061195697 40.0 37.0 41.0 33.0 41.0 7 38.03337179579735 40.0 37.0 41.0 33.0 41.0 8 38.10529741926248 40.0 37.0 41.0 33.0 41.0 9 38.081078303132344 40.0 37.0 41.0 33.0 41.0 10 37.78981752150818 39.0 37.0 41.0 32.0 41.0 11 37.83439645541679 39.0 37.0 40.0 33.0 41.0 12 37.82960353479425 39.0 37.0 40.0 33.0 41.0 13 37.78851681315441 39.0 37.0 40.0 33.0 41.0 14 37.68713742601686 39.0 36.0 40.0 33.0 41.0 15 37.640667998781275 39.0 36.0 40.0 32.0 41.0 16 37.53499125723312 39.0 36.0 40.0 32.0 41.0 17 37.42526922705131 39.0 36.0 40.0 32.0 41.0 18 37.406221130209104 39.0 36.0 40.0 32.0 41.0 19 37.33502356080842 39.0 36.0 40.0 32.0 41.0 20 37.18901507133094 38.0 36.0 40.0 31.0 41.0 21 37.08942584065772 38.0 35.0 40.0 31.0 41.0 22 37.01073114982374 38.0 35.0 40.0 31.0 41.0 23 36.856186685298326 38.0 35.0 40.0 30.0 41.0 24 36.91280848996693 38.0 35.0 40.0 31.0 41.0 25 36.945757063301805 38.0 35.0 40.0 31.0 41.0 26 37.021353587562984 38.0 35.0 40.0 31.0 41.0 27 37.07761423958535 38.0 35.0 40.0 31.0 41.0 28 36.94491130742276 38.0 35.0 40.0 31.0 41.0 29 37.03215603926679 38.0 35.0 40.0 31.0 41.0 30 37.1312619278715 39.0 35.0 40.0 31.0 41.0 31 37.12150737435018 39.0 35.0 40.0 31.0 41.0 32 37.12316546301269 38.0 35.0 40.0 31.0 41.0 33 37.04460884149184 38.0 35.0 40.0 31.0 41.0 34 37.04317777034727 38.0 35.0 40.0 31.0 41.0 35 36.98708021024648 38.0 35.0 40.0 31.0 41.0 36 36.92591555925043 38.0 35.0 40.0 31.0 41.0 37 36.85758306764059 38.0 35.0 40.0 31.0 41.0 38 36.79307827455392 38.0 35.0 40.0 31.0 41.0 39 36.69517573146652 38.0 35.0 40.0 31.0 41.0 40 36.63025509373401 38.0 35.0 40.0 31.0 41.0 41 36.50085299992493 38.0 35.0 40.0 31.0 41.0 42 36.36999282531812 38.0 34.0 40.0 31.0 41.0 43 36.24572322438927 37.0 34.0 40.0 31.0 41.0 44 36.16209131658597 37.0 34.0 40.0 30.0 41.0 45 36.10749333458066 37.0 34.0 40.0 30.0 41.0 46 36.0602513461572 37.0 34.0 40.0 30.0 41.0 47 36.01679566164702 37.0 34.0 40.0 30.0 41.0 48 36.036466385781466 37.0 34.0 40.0 31.0 41.0 49 36.01302730184232 37.0 34.0 40.0 31.0 41.0 50 36.182347498166834 37.0 34.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 4.0 22 12.0 23 17.0 24 77.0 25 144.0 26 380.0 27 953.0 28 2433.0 29 5489.0 30 11149.0 31 20031.0 32 32205.0 33 47057.0 34 65184.0 35 76816.0 36 95861.0 37 113685.0 38 126171.0 39 135610.0 40 83968.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 58.560508634476484 14.285399640500362 27.154091725023154 2 25.344234974248913 28.297809597343278 28.94975448059155 17.408200947816265 3 29.696407573230616 18.785997379005366 25.620161346569642 25.897433701194377 4 20.780376067455737 21.540366621107204 28.44709127106003 29.232166040377024 5 21.343119032556864 27.447638229323573 25.20682241721291 26.002420320906655 6 27.20829810326621 23.005040091918357 24.89724648729209 24.889415317523344 7 27.13365726640783 21.730517212054618 26.605665117155525 24.53016040438203 8 29.884845095791114 20.51289267504194 24.19770277529315 25.404559453873798 9 29.73164783718998 22.81317643258403 23.2960169936384 24.159158736587592 10 25.416183846499287 21.604361961561192 28.37636601908603 24.603088172853493 11 25.476630688151808 25.9445430818345 23.96790688739145 24.610919342622243 12 25.75891988590965 21.288423206203266 28.42017162497996 24.53248528290713 13 25.00761703622039 20.244919834517596 22.98754232196631 31.759920807295718 14 24.8135508603886 27.4510643660974 22.62473891002353 25.110645863490475 15 22.691181491030253 27.780585306522998 24.30513663555816 25.223096566888593 16 24.178002488843642 25.59911507781613 21.19224665248083 29.0306357808594 17 29.47236270062784 24.436186367157053 21.52984348673045 24.561607445484658 18 25.316091707892475 22.853555901704137 21.604484323588828 30.22586806681456 19 25.04126659382047 26.19832192715299 23.06095953854832 25.699451940478212 20 29.498548174542087 20.810599488282 24.011223045174837 25.67962929200107 21 27.20523905257529 27.71132839888063 21.601792358980823 23.48164018956325 22 29.849971917914658 22.40448726027749 23.267017193088506 24.478523628719348 23 26.34393274004065 23.551998355454348 25.79305889162028 24.311010012884722 24 30.340888372793046 23.13645690960016 23.260776729679034 23.26187798792776 25 26.60211976485601 24.458444060384185 22.62263601618009 26.316800158579724 26 26.369940198936202 26.921497330488524 22.872404363374102 23.836158107201168 27 29.461664649316187 23.479809420457578 23.106947400906353 23.95157852931988 28 27.86772666414693 23.892351746625582 24.34564800994228 23.894273579285205 29 27.343340376159603 24.3025525486552 23.614579169500235 24.739527905684962 30 26.990882095978353 23.807671299625515 24.244639088969315 24.956807515426824 31 26.07822740314879 23.99429851030715 25.81207847621468 24.115395610329386 32 26.317273035746254 23.89126235381816 25.15730328072524 24.63416132971035 33 24.391203106185205 22.945956648012075 26.852203480538368 25.81063676526435 34 25.717912148652616 23.13941982899217 27.42294070682325 23.719727315531962 35 25.493764857068797 23.57827158859639 26.04861260486126 24.879350949473555 36 24.53290777356767 24.884105983320172 24.745966164099524 25.837020079012635 37 23.868486310497815 26.09791550689676 26.576303019056773 23.457295163548643 38 25.864789588579594 24.60213642619444 24.312961866170127 25.220112119055837 39 24.953796445880453 25.27905223478729 25.214719080954946 24.55243223837731 40 24.69239893938689 25.249017304740196 24.860009536214356 25.19857421965856 41 23.981441547793754 25.908235165970677 24.77512154696946 25.33520173926611 42 23.7083708102319 26.558554204646985 25.212816577244173 24.520258407876934 43 24.10652151549442 25.509521763598848 24.482762794018868 25.90119392688786 44 22.728871157711758 25.8762574726623 24.995431560164434 26.399439809461505 45 24.573335204328234 24.721767466516987 25.76578262157569 24.939114707579087 46 24.174015714292516 24.897838054868306 24.591391894015015 26.336754336824164 47 24.07915533078612 25.575242270688364 25.74426253483862 24.601339863686903 48 24.65441066276134 24.25869261282043 24.91411248940041 26.172784235017822 49 24.924954285533087 25.20241203268188 23.780507139912693 26.09212654187234 50 22.137208003072733 27.65677921715144 25.66997800202521 24.536034777750622 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4152.0 1 4129.5 2 2758.5 3 1403.5 4 1367.5 5 1066.0 6 611.5 7 471.5 8 524.5 9 547.0 10 572.0 11 626.5 12 674.0 13 779.0 14 944.5 15 1099.5 16 1270.5 17 1482.5 18 1669.0 19 1900.0 20 2198.0 21 2564.5 22 2965.0 23 3653.0 24 4389.0 25 5007.5 26 5724.5 27 6725.0 28 7995.5 29 9234.0 30 10341.0 31 12396.5 32 15196.5 33 16840.5 34 18156.0 35 20525.5 36 22574.0 37 23884.0 38 25470.5 39 27753.0 40 30869.0 41 35219.5 42 40664.5 43 45998.0 44 49221.5 45 51543.5 46 54890.5 47 57556.0 48 64265.0 49 72424.5 50 81272.5 51 88392.0 52 89094.0 53 84765.5 54 75447.5 55 63498.0 56 53536.0 57 47951.0 58 43987.5 59 41248.5 60 39912.0 61 38795.5 62 36602.5 63 34239.5 64 31520.5 65 28748.0 66 26215.5 67 24364.0 68 21936.5 69 18622.5 70 15673.5 71 13317.0 72 11699.5 73 10776.0 74 9583.5 75 7914.5 76 6538.0 77 5167.0 78 4110.5 79 3566.0 80 2926.5 81 2149.5 82 1537.0 83 1110.0 84 857.0 85 680.0 86 512.5 87 353.0 88 224.0 89 154.0 90 100.0 91 57.0 92 43.5 93 39.0 94 26.0 95 9.5 96 4.5 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 10082.0 25 8517.0 26 8007.0 27 10136.0 28 9981.0 29 7081.0 30 7851.0 31 7038.0 32 8611.0 33 7228.0 34 7471.0 35 7131.0 36 6765.0 37 7275.0 38 7698.0 39 8471.0 40 8537.0 41 8954.0 42 9401.0 43 9805.0 44 9837.0 45 11243.0 46 28424.0 47 74560.0 48 148707.0 49 149324.0 50 229112.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.484757940548533 #Duplication Level Percentage of deduplicated Percentage of total 1 63.51307893384398 18.72667758423342 2 11.368621394505208 6.704020998694549 3 5.835659617581022 5.161890337434315 4 3.901298145523049 4.60115325898632 5 2.81327461336765 4.147436049771772 6 2.175324971857391 3.848335814234743 7 1.733322551500708 3.577461710472464 8 1.3818155795972484 3.2593998306322916 9 1.1067264773900787 2.9368406063018195 >10 5.812670902531396 28.16689176267583 >50 0.19058752634036227 3.8935425273755015 >100 0.14318498771675667 8.271508485731506 >500 0.01710400877162622 3.400761998401473 >1k 0.0073302894735540955 3.304079035053986 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTGCAG 3157 0.38629692124902265 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 2048 0.25059743259993617 Illumina Single End Adapter 1 (100% over 32bp) CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 2015 0.24655948568792543 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 1711 0.20936142928637244 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGA 1654 0.2023867937110812 No Hit ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG 1570 0.19210838338959946 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA 1355 0.16580054744771164 Illumina Single End Adapter 1 (100% over 32bp) AAGCAGTGGTATCAACGCAGAGTGCAGT 1346 0.16469928919898147 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGAAGCAGTGGT 1312 0.16053898025933408 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 1296 0.15858118781714708 No Hit AAGCAGTGGTATCAACGCAGAGTG 1268 0.15515505104331984 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGC 1099 0.13447586837271963 No Hit AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 1059 0.12958138726725213 Illumina Single End Adapter 2 (100% over 32bp) ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC 1035 0.12664469860397162 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA 1006 0.12309619980250769 Illumina Single End Adapter 1 (100% over 32bp) ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 995 0.12175021749850413 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA 990 0.1211384073603207 Illumina Single End Adapter 1 (100% over 32bp) ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA 906 0.11085999703883892 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTG 867 0.10608787796100813 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG 861 0.10535370579518799 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAC 818 0.10009213860681042 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.46473098096413934 0.0 0.0 0.0 0.0 2 1.0857182712203288 0.0 0.0 0.0 0.0 3 1.3498978888879372 0.0 0.0 0.0 0.0 4 1.5689259183576079 0.0 0.0 0.0 0.0 5 1.9993955315834748 0.0 0.0 0.0 0.0 6 2.1760862994908514 0.0 0.0 0.0 0.0 7 2.32316545671015 0.0 0.0 0.0 0.0 8 2.4554388085854093 0.0 0.0 0.0 0.0 9 2.5752312336417265 0.0 0.0 0.0 0.0 10 2.6674922024797887 0.0 0.0 0.0 0.0 11 2.79083312633757 0.0 0.0 0.0 0.0 12 2.878199614070165 0.0 0.0 0.0 0.0 13 2.943051488717609 0.0 0.0 0.0 0.0 14 3.0322534068647546 0.0 0.0 0.0 0.0 15 3.1936489213175454 0.0 0.0 0.0 0.0 16 3.260825674490087 0.0 0.0 0.0 0.0 17 3.3730316538329292 0.0 0.0 0.0 0.0 18 3.4704318278317325 0.0 0.0 0.0 0.0 19 3.857707645301849 0.0 0.0 0.0 0.0 20 3.9239055022532967 0.0 0.0 0.0 0.0 21 4.105980199376688 0.0 0.0 0.0 0.0 22 4.155903906652457 0.0 0.0 0.0 0.0 23 4.19860825429766 0.0 0.0 0.0 0.0 24 4.2290763991791955 0.0 0.0 0.0 0.0 25 4.29576370424119 0.0 0.0 0.0 0.0 26 4.609744667156931 0.0 0.0 0.0 0.0 27 4.639968087983192 0.0 0.0 0.0 0.0 28 4.666398285952717 0.0 0.0 0.0 0.0 29 4.689524709176051 0.0 0.0 0.0 0.0 30 4.7104486159019245 0.0 0.0 0.0 0.0 31 4.730760712489615 0.0 0.0 0.0 0.0 32 4.751806981243125 0.0 0.0 0.0 0.0 33 4.773587422162455 0.0 0.0 0.0 0.0 34 4.7907181060315915 0.0 0.0 0.0 0.0 35 4.809561858287641 0.0 0.0 0.0 0.0 36 4.825224197825137 0.0 0.0 0.0 0.0 37 4.844435036164097 0.0 0.0 0.0 0.0 38 4.855447618651399 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCGCA 60 0.0 127.368195 44 TAATCGC 60 0.0 77.2009 43 ACTTCTA 235 0.0 76.864365 44 TCGTGCG 170 0.0 69.35695 43 CTATCAT 375 0.0 59.284103 44 CGCTACC 85 1.8195351E-8 57.21352 44 TAATTCC 110 3.8198777E-11 56.842003 44 CATGACC 210 0.0 56.2405 44 GGTGCGT 50 2.477768E-4 55.578846 44 AACGACA 80 3.6379788E-12 52.63697 43 CTAATCG 70 0.0 52.565624 42 CCCTATC 275 0.0 51.291306 42 TCGCAAA 50 9.724827E-7 50.53149 43 GTTCAGG 440 0.0 49.76586 43 CTCGTGC 215 0.0 45.63837 42 ATGTTCT 190 0.0 43.87804 44 ACCTTAG 360 0.0 43.43965 42 ACATTGG 160 3.092282E-11 43.420975 44 CAGGCAC 420 0.0 43.007442 44 CCATGAC 170 0.0 42.109577 43 >>END_MODULE