FastQCFastQC Report
Tue 24 May 2016
ERR841208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8302469
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC786620.947453100999233No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC316800.3815732404420902No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA314720.3790679615907027No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA249530.3005491498974582No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG219610.26451167718903856No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG201730.24297591475499636No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC197820.23826647229878245No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT196420.2365802269180409No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG186130.22418632336959043No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT171060.2060350963068938No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG168410.20284327469334723No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA165240.19902513336695382No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT155940.1878236461948849No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC155770.18761888782722344No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC148660.1790551702150288No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT142290.17138275373265469No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG141980.17100937082691905No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG139540.16807048602048377Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG137680.16583018858607No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG135770.163529668102344No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC122880.14800416598965924No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC121750.14664312507520352No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA119160.14352357112083164No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA106700.12851598723223176No Hit
AAGAGCTCGTATGCCGTCTTCTGCTTG106010.12768490915172342Illumina Single End Adapter 1 (100% over 27bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA105040.12651658199506677No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG102870.12390290165491735No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC102290.12320431428289584No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC98960.11919345919870342No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA97770.11776015062507308No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG93580.11271345909271085No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC92110.11094290144293222No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT91380.11006364492297412No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC91360.11003955570324923No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG90130.10855806869016917No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAGG87850.1058118976415329No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT86900.10466765970460112No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA86690.1044147228974899Illumina Single End Adapter 1 (100% over 32bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC84870.10222260390252587No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT84440.10170468567844096No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC83950.10111449979518142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCA11400.0171.7201244
TAAGCCA41300.0159.9027444
CTATACG3750.0132.0796744
ACTTCTA23050.0112.04465544
ACATTGG14500.0111.4218844
CATGACC25450.0107.0390344
CCCGAGG30300.082.5108744
CCCATAC15450.081.6720144
CGTGCGC29000.076.85670544
TTAAGCC34750.076.2776843
ATGTTCT16650.075.0774644
TCGTGCG41700.073.95618443
ACCACTC9450.072.37926544
CCAGAAT17550.071.8992944
ATCCTTG43250.068.4279243
TAATTCC9350.068.1083144
CAGGCAC43700.065.3058244
CCTTAGC127800.064.7106843
AATCCCG28750.064.29703543
CACATGC75850.063.27858744
Produced by FastQC (version 0.11.5)