##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841208.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8302469
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.241437697629465	38.0	35.0	39.0	31.0	39.0
2	36.894734325415726	38.0	35.0	39.0	33.0	39.0
3	37.12903872330026	39.0	37.0	39.0	33.0	39.0
4	37.663993686697296	39.0	37.0	40.0	33.0	41.0
5	38.11159788732725	40.0	37.0	41.0	33.0	41.0
6	38.14189682611281	40.0	37.0	41.0	33.0	41.0
7	38.10598004039521	40.0	37.0	41.0	33.0	41.0
8	38.04690026545116	39.0	37.0	41.0	33.0	41.0
9	37.98486919975251	39.0	37.0	41.0	33.0	41.0
10	37.80261823320268	39.0	37.0	40.0	33.0	41.0
11	37.7274298765825	39.0	36.0	40.0	33.0	41.0
12	37.68983660161815	39.0	36.0	40.0	33.0	41.0
13	37.53483018123886	39.0	36.0	40.0	32.0	41.0
14	37.47991326435546	39.0	36.0	40.0	32.0	41.0
15	37.44114925331248	39.0	36.0	40.0	32.0	41.0
16	37.39040470973153	39.0	36.0	40.0	32.0	41.0
17	37.31563478285797	39.0	36.0	40.0	32.0	41.0
18	37.22575983120202	39.0	36.0	40.0	31.0	41.0
19	37.1784977456706	38.0	36.0	40.0	31.0	41.0
20	37.067456319318985	38.0	35.0	40.0	31.0	41.0
21	36.88974954317806	38.0	35.0	40.0	31.0	41.0
22	36.91976687898504	38.0	35.0	40.0	31.0	41.0
23	36.877782380157036	38.0	35.0	40.0	31.0	41.0
24	36.86229012116757	38.0	35.0	40.0	31.0	41.0
25	36.86353578842928	38.0	35.0	40.0	31.0	41.0
26	36.86197793330447	38.0	35.0	40.0	31.0	41.0
27	36.87001578915857	38.0	35.0	40.0	31.0	41.0
28	36.85787659555228	38.0	35.0	40.0	31.0	41.0
29	36.900947039183684	38.0	35.0	40.0	31.0	41.0
30	36.89708863145711	38.0	35.0	40.0	31.0	41.0
31	36.85974653729852	38.0	35.0	40.0	31.0	41.0
32	36.80755604191657	38.0	35.0	40.0	31.0	41.0
33	36.718555992756606	38.0	35.0	40.0	31.0	41.0
34	36.66284279682688	38.0	35.0	40.0	31.0	41.0
35	36.56912002027305	38.0	35.0	40.0	31.0	41.0
36	36.44370357326462	38.0	35.0	40.0	31.0	41.0
37	36.40678321917965	38.0	34.0	40.0	31.0	41.0
38	36.32228170572109	37.0	34.0	40.0	31.0	41.0
39	36.17191865849221	37.0	34.0	40.0	31.0	41.0
40	36.07577246416701	37.0	34.0	40.0	31.0	41.0
41	35.92635473967318	37.0	34.0	40.0	30.0	41.0
42	35.76858994606066	36.0	34.0	40.0	30.0	41.0
43	35.59875722771219	36.0	34.0	39.0	30.0	41.0
44	35.51502601372082	36.0	34.0	39.0	30.0	41.0
45	35.45599630263509	36.0	34.0	39.0	30.0	41.0
46	35.342279852300585	35.0	34.0	39.0	30.0	40.0
47	35.27034661622037	35.0	33.0	39.0	30.0	40.0
48	35.25379785374183	35.0	33.0	39.0	30.0	40.0
49	35.32518372597421	36.0	33.0	39.0	30.0	40.0
50	35.70754501660286	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	9.0
21	23.0
22	87.0
23	300.0
24	801.0
25	1972.0
26	4949.0
27	11990.0
28	28275.0
29	63228.0
30	126122.0
31	225013.0
32	359465.0
33	530048.0
34	736318.0
35	833701.0
36	1019641.0
37	1184350.0
38	1265494.0
39	1241380.0
40	669302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.659605835324406	18.767031831133608	33.573362333541986
2	26.582718947821427	19.759917200533963	31.023361845735288	22.634002005909327
3	28.03221547710687	17.389429578117063	25.727551647588204	28.85080329718786
4	21.064011199560035	20.324074681880774	30.42263692884611	28.189277189713085
5	24.177350135242904	19.41355035471978	27.383059183960818	29.026040326076497
6	26.70975344804058	17.45067641926757	25.615874024943665	30.22369610774819
7	28.191649977855988	18.3330645377899	23.327675177106954	30.14761030724716
8	28.37793191398848	15.892959070368104	27.529316881520426	28.19979213412299
9	27.74463837203126	19.6670050800551	24.343878911200996	28.244477636712645
10	28.568730578819384	17.038389423676257	27.717550044450633	26.675329953053723
11	29.772986806695695	18.17778542744333	23.893061208659738	28.15616655720124
12	28.801854002706907	16.231713722749223	23.846063140976497	31.120369133567376
13	26.871922075228465	17.935857393746364	25.472061383186134	29.720159147839038
14	27.133266020023683	19.290358085046748	21.016085696917386	32.56029019801218
15	25.479721755058648	21.576954999771754	23.121375099383087	29.821948145786514
16	28.33866648583692	21.22498741037154	24.780447840274984	25.65589826351655
17	28.310445964929226	21.076561683036697	19.77751437554299	30.835477976491088
18	25.76959938061798	19.61180463305554	21.438333584864935	33.18026240146154
19	27.592370414150295	18.11997130010362	25.682324137554748	28.605334148191343
20	26.773336943504393	17.30918838721349	25.302136027246835	30.61533864203528
21	27.796646997417273	21.868000952487748	22.590641410404544	27.744710639690435
22	29.56373579955553	18.59060238586859	24.71260055291986	27.13306126165602
23	28.468278532566636	20.20353222637748	25.541510603652963	25.78667863740292
24	31.318225939777673	19.581331770103567	21.200536852350787	27.89990543776797
25	28.6912686818088	20.629071232111375	23.827221364406288	26.85243872167354
26	29.241829840931917	21.87763125784089	23.134601446831578	25.745937454395616
27	28.946807210464883	21.660068470816775	23.006080632080938	26.3870436866374
28	28.359443638537417	19.81235203584439	24.95090656421891	26.877297761399284
29	29.617505355408504	19.998834111138166	25.592935066033878	24.79072546741945
30	27.4179855894247	20.851174871131175	25.234084950795538	26.496754588648585
31	27.40418858439435	21.265873392964643	27.37487470940319	23.95506331323781
32	27.5060408978261	19.0557733032679	25.160349117341585	28.277836681564413
33	25.279500623036455	18.70749782806277	28.97187853920313	27.041123009697653
34	24.78407845747911	20.94278441853793	29.152930502130754	25.120206621852205
35	27.464045024832366	19.20550744122724	24.356394281133763	28.97405325280663
36	26.259457960797683	19.175029511594154	25.256384901303758	29.309127626304406
37	24.235321450341328	22.05122331028673	26.22488207537624	27.488573163995706
38	29.733374924602213	21.83738957082601	21.621485156707624	26.807750347864147
39	26.540541885416612	21.765933882704804	25.396574696499275	26.296949535379312
40	25.83292760523957	20.677565453685478	22.96452487596941	30.524982065105544
41	25.925951395481086	23.288088799718416	23.203279859118638	27.58267994568186
42	25.790395692344543	22.109568351877375	25.974771927703628	26.12526402807445
43	23.928256461050164	23.032833699754267	24.197812446191126	28.84109739300444
44	23.662278214490325	22.14968722463117	22.73415983970081	31.453874721177698
45	24.399719809065765	20.792298374754047	28.20063097489261	26.607350841287573
46	24.365382727622716	21.168796476452233	25.230590549624566	29.235230246300485
47	27.40167980714406	22.352323195127905	23.559617724101514	26.68637927362652
48	24.30314484260878	22.509306311028528	25.42796475842825	27.759584087934435
49	27.87649187838552	20.66911271330364	20.201571085191226	31.25282432311961
50	23.215166017839934	25.441830614366534	23.07349975065306	28.269503617140472
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3236.0
1	3457.0
2	4257.5
3	4770.0
4	4493.5
5	3445.0
6	2234.0
7	1976.0
8	2167.5
9	2286.0
10	2313.0
11	2328.0
12	2320.5
13	2321.5
14	2391.5
15	2561.5
16	2756.0
17	3053.5
18	3554.5
19	4459.5
20	5491.0
21	6674.0
22	8248.5
23	10764.5
24	13482.0
25	16383.0
26	20493.0
27	24715.0
28	28941.0
29	33249.5
30	39259.0
31	55210.5
32	75168.5
33	83359.5
34	96695.0
35	122526.5
36	138460.0
37	151835.5
38	169059.5
39	186945.5
40	216113.5
41	263674.0
42	326895.5
43	395400.5
44	428657.0
45	416930.0
46	417108.5
47	431303.5
48	485904.5
49	559839.5
50	646888.0
51	741328.5
52	777086.0
53	778767.5
54	749807.0
55	699423.0
56	660577.0
57	647038.0
58	642413.5
59	610066.0
60	579566.5
61	571027.5
62	550182.0
63	520680.0
64	508608.0
65	502835.0
66	452026.0
67	389732.0
68	338135.0
69	286729.0
70	250212.0
71	224845.5
72	204287.0
73	180441.0
74	151463.0
75	125168.5
76	106611.0
77	84024.0
78	64713.5
79	52194.0
80	40346.0
81	29934.5
82	22529.5
83	17157.0
84	12807.0
85	9482.5
86	6916.0
87	4897.0
88	3327.0
89	2353.0
90	1656.5
91	1091.5
92	801.0
93	669.5
94	436.0
95	182.0
96	108.5
97	100.0
98	57.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	76151.0
25	71948.0
26	70342.0
27	77866.0
28	63724.0
29	59616.0
30	63999.0
31	64181.0
32	73711.0
33	65086.0
34	70853.0
35	71658.0
36	61396.0
37	64242.0
38	72837.0
39	73813.0
40	75618.0
41	77882.0
42	84243.0
43	86163.0
44	91767.0
45	133214.0
46	482165.0
47	1072378.0
48	1519325.0
49	2180640.0
50	1397651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.986294587517023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.444527818617715	9.417970827928231
2	12.600719907799649	4.280790807373504
3	7.023703177401816	3.579200737998798
4	4.710591353687881	3.200619696606118
5	3.429287796134222	2.912544636525646
6	2.6504103924623634	2.7012391022509394
7	2.0612400733104765	2.450898177059286
8	1.6468932899002908	2.2379691661161134
9	1.3828696549546848	2.1140848201678466
>10	8.157848999402646	24.612827021806684
>50	0.49009350522446404	5.795170269228187
>100	0.3329313056537428	11.123471759517969
>500	0.03264078411865449	3.8362312108983594
>1k	0.030035604655334034	10.023912185332476
>5k	0.004060936343687735	4.921221019106025
>10k+	0.002145400332514275	6.791848562083827
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	78662	0.947453100999233	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	31680	0.3815732404420902	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	31472	0.3790679615907027	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	24953	0.3005491498974582	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	21961	0.26451167718903856	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	20173	0.24297591475499636	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	19782	0.23826647229878245	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	19642	0.2365802269180409	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	18613	0.22418632336959043	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	17106	0.2060350963068938	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	16841	0.20284327469334723	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	16524	0.19902513336695382	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	15594	0.1878236461948849	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	15577	0.18761888782722344	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	14866	0.1790551702150288	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	14229	0.17138275373265469	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	14198	0.17100937082691905	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	13954	0.16807048602048377	Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	13768	0.16583018858607	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	13577	0.163529668102344	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	12288	0.14800416598965924	No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC	12175	0.14664312507520352	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	11916	0.14352357112083164	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10670	0.12851598723223176	No Hit
AAGAGCTCGTATGCCGTCTTCTGCTTG	10601	0.12768490915172342	Illumina Single End Adapter 1 (100% over 27bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	10504	0.12651658199506677	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	10287	0.12390290165491735	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	10229	0.12320431428289584	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	9896	0.11919345919870342	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	9777	0.11776015062507308	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	9358	0.11271345909271085	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	9211	0.11094290144293222	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT	9138	0.11006364492297412	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	9136	0.11003955570324923	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	9013	0.10855806869016917	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAGG	8785	0.1058118976415329	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8690	0.10466765970460112	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	8669	0.1044147228974899	Illumina Single End Adapter 1 (100% over 32bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	8487	0.10222260390252587	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	8444	0.10170468567844096	No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC	8395	0.10111449979518142	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.17264743776821087	0.0	0.0	0.0	0.0
2	0.39051034096002046	0.0	0.0	0.0	0.0
3	0.505789301953431	0.0	0.0	0.0	0.0
4	0.599604768171974	0.0	0.0	0.0	0.0
5	0.7480184508969561	0.0	0.0	0.0	0.0
6	0.8419061847746736	0.0	0.0	0.0	0.0
7	0.935625294114317	0.0	0.0	0.0	0.0
8	1.0136141429736143	0.0	0.0	0.0	0.0
9	1.0878571181656926	0.0	0.0	0.0	0.0
10	1.1409015799998772	0.0	0.0	0.0	0.0
11	1.196174294658613	0.0	0.0	0.0	0.0
12	1.2427146671670801	0.0	0.0	0.0	0.0
13	1.275608496701403	0.0	0.0	0.0	0.0
14	1.3106342221813776	0.0	0.0	0.0	0.0
15	1.3530432935070278	0.0	0.0	0.0	1.2044609862439715E-5
16	1.379481212155083	0.0	0.0	0.0	1.2044609862439715E-5
17	1.4079064914304407	0.0	0.0	0.0	1.2044609862439715E-5
18	1.44053533954779	0.0	0.0	0.0	1.2044609862439715E-5
19	1.5165368277797846	0.0	0.0	0.0	1.2044609862439715E-5
20	1.5388314006351604	0.0	0.0	0.0	1.2044609862439715E-5
21	1.5826015128752664	0.0	0.0	0.0	1.2044609862439715E-5
22	1.5961516989705111	0.0	0.0	0.0	1.2044609862439715E-5
23	1.609846420384105	0.0	0.0	0.0	1.2044609862439715E-5
24	1.6228184652059525	0.0	0.0	0.0	1.2044609862439715E-5
25	1.6413551197842473	0.0	0.0	0.0	1.2044609862439715E-5
26	1.7107320725919	0.0	0.0	0.0	1.2044609862439715E-5
27	1.725306050525452	0.0	0.0	0.0	1.2044609862439715E-5
28	1.7373506603878919	0.0	0.0	0.0	1.2044609862439715E-5
29	1.7494675379095062	0.0	0.0	0.0	1.2044609862439715E-5
30	1.7621264228749303	0.0	0.0	0.0	1.2044609862439715E-5
31	1.773761516002047	0.0	0.0	0.0	1.2044609862439715E-5
32	1.7847341555867298	0.0	0.0	0.0	3.613382958731914E-5
33	1.7949961631895284	0.0	0.0	0.0	3.613382958731914E-5
34	1.8057881336262742	0.0	0.0	0.0	3.613382958731914E-5
35	1.8163030780361842	0.0	0.0	0.0	3.613382958731914E-5
36	1.8259989889754482	0.0	0.0	0.0	3.613382958731914E-5
37	1.8346831526862672	0.0	0.0	0.0	3.613382958731914E-5
38	1.840597056128725	0.0	0.0	0.0	3.613382958731914E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCA	1140	0.0	171.72012	44
TAAGCCA	4130	0.0	159.90274	44
CTATACG	375	0.0	132.07967	44
ACTTCTA	2305	0.0	112.044655	44
ACATTGG	1450	0.0	111.42188	44
CATGACC	2545	0.0	107.03903	44
CCCGAGG	3030	0.0	82.51087	44
CCCATAC	1545	0.0	81.67201	44
CGTGCGC	2900	0.0	76.856705	44
TTAAGCC	3475	0.0	76.27768	43
ATGTTCT	1665	0.0	75.07746	44
TCGTGCG	4170	0.0	73.956184	43
ACCACTC	945	0.0	72.379265	44
CCAGAAT	1755	0.0	71.89929	44
ATCCTTG	4325	0.0	68.42792	43
TAATTCC	935	0.0	68.10831	44
CAGGCAC	4370	0.0	65.30582	44
CCTTAGC	12780	0.0	64.71068	43
AATCCCG	2875	0.0	64.297035	43
CACATGC	7585	0.0	63.278587	44
>>END_MODULE