FastQCFastQC Report
Tue 24 May 2016
ERR841207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27652644
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA4618881.67032129007266No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1883470.6811175090526606No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1280970.4632359929126488No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG1124220.4065506358090025No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC1114660.4030934618765569No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG838210.3031211048028536No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT810940.29325947999764507No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG717800.25957734819137No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG555550.2009030311893503No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC521560.18861125901740175No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC513470.18568567982143047No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT511360.18492264247859988No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG511290.18489732844352968No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA501510.18136059611514907No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA483800.17495614524238623No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC466310.16863125276555832No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG444950.16090685577842032No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA436320.157785996883336No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG409870.14822090791752138No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA409310.1480183956369597No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC407690.1474325565396206No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT399720.1445503728323411No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC386880.1399070555423199No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC385640.1394586354925048No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG365340.13211756532214425No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA321300.11619142097225857No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA318100.11503420794047758No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG309930.11207969841871179No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC289800.10480010519066459No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT289500.10469161646893513No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG288200.10422149867477411No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG288090.10418171947680663No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC286400.10357056634439729No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG281110.10165754855123436Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAG278980.10088727862695517No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT277780.10045332374003729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA542500.0181.2688644
AATCGCA19500.096.7738444
ATTAGTC32100.092.3809144
CGTGCGC97150.091.3670844
TTAAGCC537500.085.97877543
ATGTTCT67300.078.18552444
CACATGC152900.074.9008544
TCGTGCG207900.073.74982543
ACATTGG40550.072.6376844
AATCCCG106200.064.4393643
ACTTCTA52550.061.9406444
ATTAAGC736300.061.48341842
CATGACC44550.058.943944
CCAGAAT66350.058.83918844
CTATACG6550.056.40157744
ACACATG98100.055.68886643
ATCCTTG94050.055.07503543
TATTAGT26150.054.1626643
CTATCAT67000.052.00935444
CCTTAGC250800.050.8619443
Produced by FastQC (version 0.11.5)