##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841207.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27652644
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.33845287994884	38.0	35.0	39.0	32.0	39.0
2	36.94823399165736	39.0	37.0	39.0	33.0	39.0
3	37.20613019861681	39.0	37.0	39.0	33.0	39.0
4	37.72001161986535	39.0	37.0	40.0	33.0	41.0
5	38.24031752623728	40.0	37.0	41.0	33.0	41.0
6	38.2754228130952	40.0	37.0	41.0	34.0	41.0
7	38.2052579131312	40.0	37.0	41.0	33.0	41.0
8	38.10017599040439	40.0	37.0	41.0	33.0	41.0
9	38.078856654719885	40.0	37.0	41.0	33.0	41.0
10	37.90984721027038	39.0	37.0	41.0	33.0	41.0
11	37.836619203574166	39.0	37.0	41.0	33.0	41.0
12	37.804831176360565	39.0	37.0	40.0	33.0	41.0
13	37.69393534303627	39.0	36.0	40.0	33.0	41.0
14	37.6604559043251	39.0	36.0	40.0	33.0	41.0
15	37.62502258373557	39.0	36.0	40.0	33.0	41.0
16	37.558767544976895	39.0	36.0	40.0	32.0	41.0
17	37.512036462046815	39.0	36.0	40.0	32.0	41.0
18	37.40706711445025	39.0	36.0	40.0	32.0	41.0
19	37.29720839714278	39.0	36.0	40.0	32.0	41.0
20	37.17628502359485	38.0	35.0	40.0	31.0	41.0
21	36.960427617699054	38.0	35.0	40.0	31.0	41.0
22	36.99514176655223	38.0	35.0	40.0	31.0	41.0
23	36.946198490097366	38.0	35.0	40.0	31.0	41.0
24	36.908578868624645	38.0	35.0	40.0	31.0	41.0
25	36.922193559139764	38.0	35.0	40.0	31.0	41.0
26	36.952223224084904	38.0	35.0	40.0	31.0	41.0
27	36.96003275467167	38.0	35.0	40.0	31.0	41.0
28	36.96215339814639	38.0	35.0	40.0	31.0	41.0
29	36.98546104774938	38.0	35.0	40.0	31.0	41.0
30	36.99023067811213	38.0	35.0	40.0	31.0	41.0
31	36.94931847416689	38.0	35.0	40.0	31.0	41.0
32	36.93184273944504	38.0	35.0	40.0	31.0	41.0
33	36.87501333052269	38.0	35.0	40.0	31.0	41.0
34	36.77911835133579	38.0	35.0	40.0	31.0	41.0
35	36.69912492509558	38.0	35.0	40.0	31.0	41.0
36	36.57555085241573	38.0	35.0	40.0	31.0	41.0
37	36.543550882128514	38.0	35.0	40.0	31.0	41.0
38	36.466269319701745	38.0	35.0	40.0	31.0	41.0
39	36.29995973949625	37.0	34.0	40.0	31.0	41.0
40	36.165032735607795	37.0	34.0	40.0	31.0	41.0
41	36.025713974582125	37.0	34.0	40.0	30.0	41.0
42	35.86090739751062	37.0	34.0	40.0	30.0	41.0
43	35.72891294291311	36.0	34.0	40.0	30.0	41.0
44	35.6125142739835	36.0	34.0	39.0	30.0	41.0
45	35.5691662390415	36.0	34.0	39.0	30.0	41.0
46	35.48730702962176	36.0	34.0	39.0	30.0	41.0
47	35.41822727614293	36.0	34.0	39.0	30.0	40.0
48	35.399520548023695	36.0	34.0	39.0	30.0	40.0
49	35.497753832026	36.0	34.0	39.0	30.0	40.0
50	35.965597534953005	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	36.0
21	128.0
22	357.0
23	979.0
24	2655.0
25	6672.0
26	16424.0
27	40147.0
28	93797.0
29	203302.0
30	401560.0
31	709309.0
32	1131148.0
33	1677746.0
34	2351531.0
35	2663107.0
36	3309970.0
37	3897726.0
38	4284222.0
39	4364788.0
40	2497039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.18601157994151	18.61646936907733	29.197519050981164
2	26.837900202237442	25.26925815846036	27.800104756709704	20.092736882592494
3	28.977493074441636	18.47674674436195	23.926876576431532	28.618883604764882
4	20.304369448360887	20.19816983865991	27.8501650692064	31.6472956437728
5	23.802338756467556	20.293238505511443	28.51236576148017	27.392056976540836
6	29.532673259020005	18.55972976761282	24.27693351854528	27.630663454821896
7	32.97954799548282	18.858471544348525	21.86207944527836	26.299901014890292
8	28.282973591964662	16.59875995944547	28.90755401183337	26.210712436756502
9	27.800726758714283	19.71714169538363	25.393673024539716	27.08845852136237
10	30.550944784882056	18.81027362157485	25.86643071092949	24.772350882613612
11	30.398409642130424	22.00995318928635	23.115811276491318	24.47582589209191
12	29.207474699345205	17.617848767011214	25.2249585970875	27.949717936556084
13	26.600483483604677	18.297024327944918	24.4122153382512	30.690276850199204
14	27.248121373131625	20.388943639530453	20.119396177812146	32.243538809525774
15	24.622538806777392	20.864605207371852	24.910124326628587	29.60273165922217
16	29.01606081501646	21.8465438603267	23.120082115836734	26.017313208820102
17	28.099982048732848	20.37612750520348	18.251997892136462	33.27189255392721
18	26.148747295195353	20.716764009980384	20.577001606067036	32.55748708875723
19	27.259932178637243	22.14749880698569	24.970028182476874	25.6225408319002
20	30.408481011797644	17.83318803077203	24.64723083984302	27.111100117587313
21	29.157038292613173	21.75203571853744	23.428302190560874	25.662623798288507
22	28.868425746196273	22.147520504730036	23.211740620535238	25.77231312853845
23	30.524896642794808	21.136051221720425	23.028857566025152	25.310194569459615
24	31.020292309118798	21.101107004451364	19.622814368130584	28.255786318299254
25	27.298625156368285	24.606968133119576	22.172059698655218	25.92234701185692
26	29.192052658344153	21.858362629994442	24.284076475558752	24.665508236102653
27	27.941303129628203	24.192620981195155	22.316496857658525	25.549579031518117
28	27.900857288482555	20.325456273752167	26.018054161736824	25.755632276028457
29	32.85407673517177	19.953441826990538	23.896285002557438	23.296196435280255
30	26.792290645397067	22.31597928927156	22.95169279258325	27.940037272748125
31	26.67733179262788	20.788239526839796	28.898638059124803	23.635790621407523
32	25.875811004728273	19.629927852241305	27.90819588766614	26.58606525536429
33	27.37794250497417	19.002503006249285	27.06389659475129	26.555657894025252
34	24.32057154077496	19.65240721386262	31.784282503221373	24.24273874214105
35	28.0022151273467	18.909197209217798	24.087782662031778	29.000805001403723
36	25.58455996454087	19.7599856619286	25.864230523900332	28.791223849630192
37	24.178983294055353	21.77083833302501	26.27525201058958	27.77492636233006
38	28.722686567902283	24.03806183869215	21.565726963713235	25.67352462969233
39	26.203122691256116	24.293713366736853	24.54823962492056	24.954924317086473
40	26.009005263785678	23.441167587582814	22.528920019522914	28.02090712910859
41	28.18496267231913	22.719707807025806	22.505011693184674	26.59031782747039
42	25.09673194455707	25.10861466079557	25.399775014374832	24.394878380272527
43	23.847089089393915	23.719316940513735	26.38741297591928	26.046180994173074
44	22.24228632717964	22.891780751613442	26.33285108971222	28.533081831494695
45	24.21579785362931	23.25570244531276	27.69367816182883	24.834821539229093
46	23.690771099642884	24.554481998517318	23.814785378455603	27.939961523384195
47	30.323324061669222	21.763295241547254	22.772066775200166	25.141313921583365
48	23.688966989548085	21.789465119983316	24.288178827336555	30.233389063132044
49	27.129187513387958	20.97545984230632	20.806314122931585	31.089038521374135
50	21.767111041705753	31.92980877016235	21.59318193566485	24.709898252467053
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37599.0
1	37485.5
2	27991.5
3	18289.0
4	17251.5
5	13769.0
6	9320.0
7	8086.5
8	8807.0
9	9327.0
10	9611.5
11	9811.5
12	10018.5
13	10356.0
14	11056.5
15	12098.5
16	13155.0
17	14941.0
18	17580.5
19	21904.0
20	27023.0
21	33449.0
22	40711.5
23	51483.5
24	63958.5
25	75747.0
26	91150.0
27	110291.5
28	129649.5
29	150662.5
30	176588.0
31	218331.0
32	270821.0
33	312819.5
34	373939.0
35	456894.0
36	507844.0
37	552684.5
38	604355.5
39	655231.0
40	736368.5
41	896534.0
42	1112622.5
43	1588862.5
44	1983847.5
45	1711823.0
46	1451613.0
47	1494501.5
48	1652670.0
49	1900318.5
50	2159988.5
51	2391083.0
52	2463980.0
53	2438798.5
54	2316938.0
55	2153407.5
56	2056456.5
57	2047775.0
58	2060153.0
59	1987591.5
60	1904167.0
61	1854445.5
62	1783658.0
63	1686945.0
64	1570552.0
65	1462066.0
66	1280948.0
67	1098488.0
68	970705.5
69	848523.0
70	753882.0
71	689807.0
72	645955.5
73	582484.5
74	484993.0
75	400551.5
76	346986.0
77	279049.0
78	222128.0
79	183037.0
80	143952.5
81	108336.0
82	81390.0
83	63527.5
84	49861.5
85	38321.5
86	28076.0
87	19099.0
88	12760.5
89	9027.5
90	6130.0
91	3890.0
92	2664.0
93	2091.5
94	1386.5
95	665.0
96	465.0
97	438.0
98	255.0
99	72.0
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	256583.0
25	229996.0
26	217848.0
27	224074.0
28	213223.0
29	181764.0
30	221635.0
31	197222.0
32	208006.0
33	197621.0
34	204095.0
35	226075.0
36	197968.0
37	206575.0
38	231616.0
39	251378.0
40	255931.0
41	266411.0
42	290202.0
43	304477.0
44	316372.0
45	448076.0
46	1492260.0
47	3359976.0
48	5156762.0
49	6763784.0
50	5532714.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.916603484108519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.323518653509794	8.964791132377051
2	12.265953037769803	3.904646217137566
3	6.961326358594285	3.3240201411965478
4	4.698196265925755	2.9911730822103797
5	3.3743095054268704	2.6853773215268912
6	2.571898486697586	2.456153304848654
7	1.9945721849165987	2.222277021142463
8	1.629595796604894	2.075010410714404
9	1.340984653038259	1.9209528900616324
>10	7.888779810228217	22.263797685025725
>50	0.52946133897727	5.850138013761712
>100	0.35303149452952864	10.916148803019384
>500	0.035025175991897806	3.8702273766013673
>1k	0.02703068192831168	8.673669208444466
>5k	0.0034584944637085593	3.849799384019572
>10k+	0.002858061397092476	14.031818007912333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	461888	1.67032129007266	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	188347	0.6811175090526606	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	128097	0.4632359929126488	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	112422	0.4065506358090025	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	111466	0.4030934618765569	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	83821	0.3031211048028536	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	81094	0.29325947999764507	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	71780	0.25957734819137	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	55555	0.2009030311893503	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	52156	0.18861125901740175	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	51347	0.18568567982143047	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	51136	0.18492264247859988	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	51129	0.18489732844352968	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	50151	0.18136059611514907	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	48380	0.17495614524238623	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	46631	0.16863125276555832	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	44495	0.16090685577842032	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	43632	0.157785996883336	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	40987	0.14822090791752138	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	40931	0.1480183956369597	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	40769	0.1474325565396206	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	39972	0.1445503728323411	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	38688	0.1399070555423199	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	38564	0.1394586354925048	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	36534	0.13211756532214425	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	32130	0.11619142097225857	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	31810	0.11503420794047758	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	30993	0.11207969841871179	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	28980	0.10480010519066459	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	28950	0.10469161646893513	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	28820	0.10422149867477411	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	28809	0.10418171947680663	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	28640	0.10357056634439729	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	28111	0.10165754855123436	Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAG	27898	0.10088727862695517	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	27778	0.10045332374003729	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.20543785975764198	0.0	0.0	0.0	0.0
2	0.5709725261714576	0.0	0.0	0.0	0.0
3	0.7602202523563388	0.0	0.0	0.0	0.0
4	0.9129904540050492	0.0	0.0	0.0	0.0
5	1.1334431528500493	0.0	0.0	0.0	0.0
6	1.3020382427083645	0.0	0.0	0.0	0.0
7	1.456942055884421	0.0	0.0	0.0	0.0
8	1.5804890121899373	0.0	0.0	0.0	0.0
9	1.6916031609852569	0.0	0.0	0.0	3.6162907243155484E-6
10	1.7834171661849043	0.0	0.0	0.0	3.6162907243155484E-6
11	1.8751479966978926	0.0	0.0	0.0	3.6162907243155484E-6
12	1.9488154550429246	0.0	0.0	7.232581448631097E-6	3.6162907243155484E-6
13	2.003587794353408	0.0	0.0	7.232581448631097E-6	3.6162907243155484E-6
14	2.0608806882987394	0.0	0.0	7.232581448631097E-6	3.6162907243155484E-6
15	2.127040727100092	0.0	0.0	7.232581448631097E-6	7.232581448631097E-6
16	2.1701469125339337	0.0	0.0	7.232581448631097E-6	7.232581448631097E-6
17	2.217393750847116	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
18	2.259783910717543	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
19	2.3804920788044717	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
20	2.4123624489578646	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
21	2.4750616975360478	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
22	2.4967522093004924	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
23	2.5166490408656763	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
24	2.5347594248130485	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
25	2.5602578907101976	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
26	2.670981480107291	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
27	2.690900009416821	0.0	0.0	7.232581448631097E-6	1.0848872172946646E-5
28	2.707686830959094	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
29	2.7230488339559864	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
30	2.73853740712823	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
31	2.753049581804908	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
32	2.766766172522237	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
33	2.780714205845922	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
34	2.7939570624783654	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
35	2.8076736531956943	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
36	2.820562113337155	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
37	2.8314326832544476	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
38	2.8392691852540395	0.0	0.0	1.808145362157774E-5	1.0848872172946646E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	54250	0.0	181.26886	44
AATCGCA	1950	0.0	96.77384	44
ATTAGTC	3210	0.0	92.38091	44
CGTGCGC	9715	0.0	91.36708	44
TTAAGCC	53750	0.0	85.978775	43
ATGTTCT	6730	0.0	78.185524	44
CACATGC	15290	0.0	74.90085	44
TCGTGCG	20790	0.0	73.749825	43
ACATTGG	4055	0.0	72.63768	44
AATCCCG	10620	0.0	64.43936	43
ACTTCTA	5255	0.0	61.94064	44
ATTAAGC	73630	0.0	61.483418	42
CATGACC	4455	0.0	58.9439	44
CCAGAAT	6635	0.0	58.839188	44
CTATACG	655	0.0	56.401577	44
ACACATG	9810	0.0	55.688866	43
ATCCTTG	9405	0.0	55.075035	43
TATTAGT	2615	0.0	54.16266	43
CTATCAT	6700	0.0	52.009354	44
CCTTAGC	25080	0.0	50.86194	43
>>END_MODULE