FastQCFastQC Report
Tue 24 May 2016
ERR841206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158600
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG180530.34995929128058No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC144840.2807738533710697No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG136680.2649556081107277No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC121380.23529639824758655No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT118600.22990733920055828No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA97550.18910169425813203No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG90490.17541581049121854No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC83080.1610514480673051No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG82560.160043422634048No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC77850.1509130384212771No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC73980.1434110029853061No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT71880.1393401310433063No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTACC69570.1348621719071066No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG69530.13478463148916373No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA66290.12850385763579264No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG66250.1284263172178498No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG64240.12452991121622145No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC62940.12200984763307875No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG54680.10599775132787966No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC52900.10254720272942272No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA52460.10169425813205132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC26250.098.0357644
CGCTACC12650.086.767244
ATTAGTC6950.069.3606144
CCAGAAT20900.064.58169644
TCGTGCG51500.061.03726243
AATCCCG8550.060.70408243
ACGACAC6050.060.06549544
CATCGTT2450.051.45969444
AATCGCA3550.050.13802744
ACGCTAC27850.049.8970943
AACGACA9100.049.21581643
TTTGGAC23750.047.40792543
TACGCTA26150.044.49317642
CTATCAT11850.044.43491744
CTCGTGC61500.043.22144742
TGTGTTA22750.042.8637342
CTAATCC29650.042.7402141
ACACATG15700.042.65624643
GAACACA18500.041.69135344
TCCAGAA47700.041.06669643
Produced by FastQC (version 0.11.5)