FastQCFastQC Report
Tue 24 May 2016
ERR841205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5244888
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG216530.4128400835251391No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG175700.334992853994213No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG148780.2836666864954981No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG145260.27695538970517575No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA125240.23878488921021765No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC124940.23821290368831516No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT122880.23428526977125158No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC103310.19697274755914712No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT93120.17754430599852658No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT92700.1767435262678631No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA92100.17559955522405815No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG90180.17193884788388236No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG87040.16595206608796984No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC81750.15586605471842296No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT81380.1551606059080766No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT80690.1538450392077009No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG80130.15277733290014964No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG76480.14581817571700292No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT73750.14061310746769046No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG73380.13990765865734406No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC71830.13695240012751464No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG66630.1270379844145385No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG65000.12393019641220174No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG62690.11952590789355273No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC59100.11268114781478653No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA57920.11043133809530348No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG56820.10833405784832775No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT52700.1004787900142005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTCT18550.0140.7744
CTATACG951.8189894E-12113.34926644
CGTGCGC23800.099.53779644
TCCTTGT25900.095.62475644
CGCTACC7850.090.8779544
CCTCGAA4050.079.7642944
TCGTGCG36000.077.3768143
ATCCTTG39550.076.64603443
ACGACAC3000.076.27460544
TAAAACC7450.063.23594744
TAAGCCA12950.060.2851744
AATCCCG13000.059.31467443
CCAGAAT8600.057.91026744
GTTCGAA1403.903442E-757.68667644
AACGACA7250.055.837643
ACGCTAC13350.055.5936243
CTCGTGC41700.054.0420542
TGTGTTA22000.053.0627942
CTAAAAC12850.051.7560943
TTCAGGA22050.051.27704644
Produced by FastQC (version 0.11.5)