FastQCFastQC Report
Tue 24 May 2016
ERR841204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172167
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA331831.0460672467748389No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG188530.5943255824803676No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC134970.425482012769189No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT126190.3978037726260944No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC116460.3671307342898404No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG111970.35297637230322365No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC105970.33406185739905875No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC99070.31231016525926913No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG92880.29279669071647235No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG85840.27060365989558555No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG74110.23362578325794325No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA66140.20850100262691088No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC63160.19910679355784233No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA59380.18719064916821845No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG55600.17527450477859458No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT55100.1736982952032475No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC54590.17209056143639348No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG54420.17155465018077548No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA54320.17123940826570605No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA50620.15957545740813772No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG50030.15771553010922817No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC48990.15443701419250627No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC48200.15194660306345786No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC47450.14958228870043727No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG47190.1487626597212568No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT47140.14860503876372208No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA46000.14501128093193077No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT45550.1435926923141184No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC45050.14201648273877132No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC44740.14103923280205613No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC43570.13735090239574396No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG43520.13719328143820927No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG41460.13069929798777935No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA40340.1271685885390019No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC40180.12666420147489083No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC38870.12253453238748148No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG38570.12158880664227324No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT38170.12032783898199559No Hit
TCAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT37250.11742761336335697No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC36300.11443281517019754No Hit
TCAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG34240.10793883171976758No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC33720.10629957376140664No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG32600.10276886431262919No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT32170.1014133240778307No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT31760.1001208322260461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA41100.0147.7399344
CGTGCGC15800.0106.1115744
ACATTGG5350.086.0566944
TTAAGCC38500.081.4098443
TCGTGCG35300.077.909943
AATCCCG13150.067.81174543
GAACACA16500.067.3889344
CCAGAAT10750.063.83827644
ACTTCTA7550.063.2817244
AATCGCA2000.060.80798344
ATTAAGC52150.059.4510942
ATTAGTC2650.059.00504744
CGCTACC5850.057.91235744
CAATGAA14800.057.26889443
GTGTTAG12850.056.68395243
AATCCTG11650.056.08091443
AATGAAG12900.055.89216244
CTCGTGC43400.052.7920942
TGTGTTA18150.052.27077542
CCTCGAA2400.050.67331344
Produced by FastQC (version 0.11.5)