##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841204.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3172167
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.343906231922844	38.0	35.0	39.0	32.0	39.0
2	36.96255083669933	39.0	37.0	39.0	33.0	39.0
3	37.19330791853014	39.0	37.0	39.0	33.0	39.0
4	37.64175372860256	39.0	37.0	40.0	33.0	41.0
5	38.22591843367641	40.0	37.0	41.0	33.0	41.0
6	38.25257276807936	40.0	37.0	41.0	33.0	41.0
7	38.196242505517525	40.0	37.0	41.0	33.0	41.0
8	38.071547305044156	40.0	37.0	41.0	33.0	41.0
9	38.025415118434815	40.0	37.0	41.0	33.0	41.0
10	37.92088436705886	39.0	37.0	41.0	33.0	41.0
11	37.79626986851575	39.0	37.0	41.0	33.0	41.0
12	37.74642602359838	39.0	36.0	40.0	33.0	41.0
13	37.639218868363486	39.0	36.0	40.0	32.0	41.0
14	37.60825580746537	39.0	36.0	40.0	32.0	41.0
15	37.60844116971143	39.0	36.0	40.0	32.0	41.0
16	37.55434597232743	39.0	36.0	40.0	32.0	41.0
17	37.521645928477284	39.0	36.0	40.0	32.0	41.0
18	37.387142291058446	39.0	36.0	40.0	32.0	41.0
19	37.24815370691392	39.0	36.0	40.0	32.0	41.0
20	37.14959426789321	38.0	35.0	40.0	31.0	41.0
21	36.937245107209044	38.0	35.0	40.0	31.0	41.0
22	36.97043125409223	38.0	35.0	40.0	31.0	41.0
23	36.93348301019461	38.0	35.0	40.0	31.0	41.0
24	36.87318101474481	38.0	35.0	40.0	31.0	41.0
25	36.8718903343513	38.0	35.0	40.0	31.0	41.0
26	36.878296586117955	38.0	35.0	40.0	31.0	41.0
27	36.89779984152155	38.0	35.0	40.0	31.0	41.0
28	36.92694540628845	38.0	35.0	40.0	31.0	41.0
29	36.93234399205091	38.0	35.0	40.0	31.0	41.0
30	36.939733239953966	38.0	35.0	40.0	31.0	41.0
31	36.88739496247389	38.0	35.0	40.0	31.0	41.0
32	36.86991107362625	38.0	35.0	40.0	31.0	41.0
33	36.834694335519075	38.0	35.0	40.0	31.0	41.0
34	36.72741933996531	38.0	35.0	40.0	31.0	41.0
35	36.62818356092048	38.0	35.0	40.0	31.0	41.0
36	36.50652375057829	38.0	35.0	40.0	31.0	41.0
37	36.475683234010496	38.0	35.0	40.0	31.0	41.0
38	36.38312380501773	37.0	34.0	40.0	31.0	41.0
39	36.237610461524866	37.0	34.0	40.0	31.0	41.0
40	36.078607661511974	37.0	34.0	40.0	31.0	41.0
41	35.914914392694186	37.0	34.0	40.0	30.0	41.0
42	35.726131005491524	36.0	34.0	39.0	30.0	41.0
43	35.61287048209158	36.0	34.0	39.0	30.0	41.0
44	35.49465146638602	36.0	34.0	39.0	30.0	41.0
45	35.42319605140345	36.0	34.0	39.0	30.0	41.0
46	35.300112938260156	35.0	34.0	39.0	30.0	40.0
47	35.262957164177486	35.0	34.0	39.0	30.0	40.0
48	35.26820951580687	35.0	33.0	39.0	30.0	40.0
49	35.341384751579	35.0	34.0	39.0	30.0	40.0
50	35.8195792013047	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	13.0
22	52.0
23	123.0
24	306.0
25	771.0
26	1966.0
27	4804.0
28	11122.0
29	24335.0
30	47851.0
31	84608.0
32	133348.0
33	196025.0
34	271473.0
35	314914.0
36	384198.0
37	448063.0
38	492031.0
39	489660.0
40	266502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.32954885414292	16.968085223760287	34.70236592209679
2	27.277567669041385	23.73046564068033	28.211503366626033	20.780463323652253
3	29.386977419536862	17.928753435742824	23.87664331669802	28.807625828022292
4	19.628033454732996	20.25971520414909	29.932314408415444	30.17993693270247
5	23.402298807093068	19.430313725601458	28.51092013755896	28.65646732974651
6	27.9788233091133	20.073060466236488	25.136759823804987	26.811356400845227
7	32.98902611369452	19.662363299284056	20.97840372212434	26.37020686489709
8	28.07929090744592	16.882560092201956	28.07866042361578	26.959488576736344
9	27.38642070231485	20.45418794155541	25.511204170524437	26.6481871856053
10	28.991601009656804	19.27218838100264	26.908293289729073	24.827917319611483
11	30.71584818832048	20.240580019904375	24.256446775973647	24.7871250158015
12	30.24944777497528	18.684514402930237	23.82756015052171	27.238477671572774
13	27.148160862905392	18.492815794376526	25.368935494253613	28.990087848464473
14	26.365635857128584	21.13063404291136	20.906717710637555	31.597012389322504
15	25.26049227546973	20.218229368125954	24.169566104180518	30.351712252223795
16	27.58341537504173	23.031006879524313	23.712465327329866	25.673112418104093
17	28.18833308586843	20.45872742513241	17.938431362535454	33.41450812646371
18	26.87131541309143	21.665000613145523	21.184571934579736	30.27911203918331
19	26.886793791121338	21.162221282801315	25.010978299692294	26.940006626385056
20	29.691343488536386	18.15377311471937	24.300391498934324	27.85449189780992
21	30.27923813594934	21.909502242473362	22.152206992885308	25.659052628692
22	27.71975750330925	22.039665629205523	22.60505200388252	27.635524863602708
23	30.751502048914826	22.10835684249915	21.77697454137818	25.36316656720784
24	30.84103075279454	22.046380281996502	19.147730872933234	27.964858092275723
25	27.324203226231376	24.929220563931857	22.28822010002348	25.458356109813284
26	30.16743173664091	21.641921576770542	22.96708717432908	25.22355951225947
27	27.311486516874655	23.476003845157038	21.552648787096267	27.659860850872036
28	26.38617240658426	21.367352443235983	24.750412486762755	27.496062663417
29	33.30600806413177	19.228125787833857	23.72772330436789	23.73814284366648
30	27.140926607062298	24.33087407123994	21.60402890479707	26.924170416900694
31	26.729956657355974	20.535235061283235	27.426905838882398	25.307902442478397
32	24.869140331288	20.36515894752459	27.838870107207537	26.926830613979874
33	26.84557320056189	19.168368343123056	26.362832674357428	27.623225781957622
34	24.30161577930126	20.167476281081186	30.01635622624394	25.514551713373613
35	28.722141959442226	19.330536126684613	24.807050915976745	27.140270997896415
36	25.551477994367826	19.893115103925712	24.684770482624867	29.8706364190816
37	25.01699726433319	21.547349722127112	25.45591529305664	27.97973772048305
38	28.351308455928436	22.128591125838803	21.62657057964695	27.893529838585813
39	26.397837472738633	23.470780731334916	24.591088409831404	25.540293386095048
40	25.680569100159513	23.20402151441092	22.36740597704923	28.74800340838034
41	27.51017384857064	23.11466380278196	22.23530712907927	27.139855219568133
42	24.878018268284382	23.890095940687868	25.772851452908114	25.459034338119636
43	23.472330803644958	24.574582333121427	26.029271150149913	25.9238157130837
44	23.530451352266844	23.11011366979229	24.89500998637141	28.464424991569455
45	25.574416697657902	22.85682660698986	27.736998712730564	23.83175798262167
46	23.81541657983611	24.513768399297238	24.199305199452713	27.471509821413935
47	30.973338627152796	21.52828239696821	22.336671935515287	25.161707040363705
48	22.6956936241228	23.357583118796004	24.140055427152678	29.806667829928518
49	28.40532044736977	21.402941734700804	20.90115389037972	29.2905839275497
50	23.299417796091596	29.784797571866427	21.20058016870481	25.715204463337166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5570.0
1	5406.0
2	3965.0
3	2631.5
4	2478.0
5	1986.5
6	1271.5
7	987.5
8	1030.0
9	1076.5
10	1126.5
11	1139.0
12	1160.0
13	1206.0
14	1387.5
15	1699.0
16	1873.5
17	1906.0
18	1983.5
19	2266.5
20	2645.0
21	3292.0
22	4113.5
23	5208.5
24	6402.0
25	7544.5
26	8859.0
27	10729.0
28	12916.0
29	15449.0
30	18503.5
31	23842.5
32	31052.0
33	36699.5
34	44647.0
35	56715.0
36	62762.0
37	65030.0
38	69870.0
39	75805.5
40	84713.5
41	105407.5
42	134658.5
43	178559.5
44	207279.0
45	185169.5
46	168883.5
47	172253.0
48	183666.0
49	206434.5
50	234661.0
51	255616.5
52	256662.5
53	255099.0
54	250037.0
55	238612.5
56	234768.5
57	239958.0
58	242056.0
59	234946.0
60	231375.5
61	235183.0
62	233385.0
63	221272.0
64	192961.5
65	161506.5
66	137552.0
67	121995.0
68	111701.0
69	99825.0
70	89395.0
71	83267.5
72	80445.0
73	74016.0
74	60849.0
75	48500.0
76	40638.0
77	31492.5
78	24756.0
79	21545.5
80	17852.0
81	13216.0
82	9421.5
83	7472.5
84	6190.0
85	4912.0
86	3698.5
87	2490.5
88	1556.0
89	1074.0
90	765.0
91	522.5
92	373.0
93	310.0
94	192.0
95	72.5
96	49.5
97	45.0
98	35.0
99	24.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	29305.0
25	27025.0
26	25196.0
27	24482.0
28	23798.0
29	20994.0
30	27318.0
31	21590.0
32	22427.0
33	22095.0
34	22896.0
35	25869.0
36	22830.0
37	24187.0
38	25069.0
39	29227.0
40	29145.0
41	30889.0
42	34227.0
43	35489.0
44	37156.0
45	48491.0
46	159660.0
47	351100.0
48	608064.0
49	706611.0
50	737027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.242493558449365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.42816237613725	12.624023564655785
2	12.01268621880389	5.1035881924523006
3	6.33266495881859	4.035647837865657
4	4.3141129765409545	3.6657006845837614
5	3.064808891217269	3.2552091564780583
6	2.4144153095410386	3.077292099620822
7	1.938634042776647	2.88269948161115
8	1.557891765058845	2.647480462721906
9	1.262804883700951	2.4142612146836258
>10	7.049220106209961	25.449888809719738
>50	0.33912325572970065	4.977766155853091
>100	0.23444553767467702	9.973617705704985
>500	0.028642464308134215	4.186328802634541
>1k	0.018930364341582958	8.603933738752415
>5k	0.0023045660937579255	3.2004504676739804
>10k+	0.0011522830468789628	3.902111624988253
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	33183	1.0460672467748389	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	18853	0.5943255824803676	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	13497	0.425482012769189	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	12619	0.3978037726260944	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	11646	0.3671307342898404	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	11197	0.35297637230322365	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	10597	0.33406185739905875	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	9907	0.31231016525926913	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	9288	0.29279669071647235	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	8584	0.27060365989558555	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	7411	0.23362578325794325	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	6614	0.20850100262691088	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	6316	0.19910679355784233	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	5938	0.18719064916821845	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	5560	0.17527450477859458	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	5510	0.1736982952032475	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	5459	0.17209056143639348	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	5442	0.17155465018077548	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5432	0.17123940826570605	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	5062	0.15957545740813772	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	5003	0.15771553010922817	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	4899	0.15443701419250627	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	4820	0.15194660306345786	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	4745	0.14958228870043727	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	4719	0.1487626597212568	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	4714	0.14860503876372208	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4600	0.14501128093193077	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	4555	0.1435926923141184	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	4505	0.14201648273877132	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	4474	0.14103923280205613	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	4357	0.13735090239574396	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	4352	0.13719328143820927	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	4146	0.13069929798777935	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	4034	0.1271685885390019	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	4018	0.12666420147489083	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	3887	0.12253453238748148	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG	3857	0.12158880664227324	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	3817	0.12032783898199559	No Hit
TCAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	3725	0.11742761336335697	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	3630	0.11443281517019754	No Hit
TCAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	3424	0.10793883171976758	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	3372	0.10629957376140664	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	3260	0.10276886431262919	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	3217	0.1014133240778307	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT	3176	0.1001208322260461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.18649711695506574	0.0	0.0	0.0	0.0
2	0.48859344416608586	0.0	0.0	0.0	0.0
3	0.6953921404516219	0.0	0.0	0.0	0.0
4	0.8599168959263495	0.0	0.0	0.0	0.0
5	1.0312193525750692	0.0	0.0	0.0	0.0
6	1.2301054767923632	0.0	0.0	0.0	0.0
7	1.4312613427981566	0.0	0.0	0.0	0.0
8	1.5755160431339208	0.0	0.0	0.0	0.0
9	1.714411630913505	0.0	0.0	0.0	0.0
10	1.8280878654875359	0.0	0.0	0.0	0.0
11	1.936814801994977	0.0	0.0	0.0	0.0
12	2.0356431423692385	0.0	0.0	0.0	0.0
13	2.1012449848951835	0.0	0.0	0.0	0.0
14	2.166594633889073	0.0	0.0	0.0	0.0
15	2.2467921770827326	0.0	0.0	0.0	0.0
16	2.301265980006727	0.0	0.0	0.0	0.0
17	2.353564613716743	0.0	0.0	0.0	0.0
18	2.398770304337697	0.0	0.0	0.0	0.0
19	2.4688801062491352	0.0	0.0	0.0	0.0
20	2.499458572010868	0.0	0.0	0.0	0.0
21	2.539462771033177	0.0	0.0	0.0	0.0
22	2.5619395195776264	0.0	0.0	0.0	0.0
23	2.58343901818536	0.0	0.0	0.0	0.0
24	2.599989218726505	0.0	0.0	0.0	0.0
25	2.6205429915890304	0.0	0.0	0.0	0.0
26	2.67826378623824	0.0	0.0	0.0	0.0
27	2.6955075189925375	0.0	0.0	0.0	0.0
28	2.711805526001626	0.0	0.0	0.0	0.0
29	2.7265273234353677	0.0	0.0	0.0	0.0
30	2.7411545482945887	0.0	0.0	0.0	0.0
31	2.7540794668124344	0.0	0.0	0.0	0.0
32	2.7669098127557596	0.0	0.0	0.0	0.0
33	2.780622836061279	0.0	0.0	0.0	0.0
34	2.794272810983785	0.0	0.0	0.0	0.0
35	2.8093728987156097	0.0	0.0	0.0	0.0
36	2.8212890431052338	0.0	0.0	0.0	0.0
37	2.8317550746855384	0.0	0.0	0.0	0.0
38	2.8388480177746	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	4110	0.0	147.73993	44
CGTGCGC	1580	0.0	106.11157	44
ACATTGG	535	0.0	86.05669	44
TTAAGCC	3850	0.0	81.40984	43
TCGTGCG	3530	0.0	77.9099	43
AATCCCG	1315	0.0	67.811745	43
GAACACA	1650	0.0	67.38893	44
CCAGAAT	1075	0.0	63.838276	44
ACTTCTA	755	0.0	63.28172	44
AATCGCA	200	0.0	60.807983	44
ATTAAGC	5215	0.0	59.45109	42
ATTAGTC	265	0.0	59.005047	44
CGCTACC	585	0.0	57.912357	44
CAATGAA	1480	0.0	57.268894	43
GTGTTAG	1285	0.0	56.683952	43
AATCCTG	1165	0.0	56.080914	43
AATGAAG	1290	0.0	55.892162	44
CTCGTGC	4340	0.0	52.79209	42
TGTGTTA	1815	0.0	52.270775	42
CCTCGAA	240	0.0	50.673313	44
>>END_MODULE