FastQCFastQC Report
Tue 24 May 2016
ERR841203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447072
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG108570.7502736560447579No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC85980.5941653214214635No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA52160.36045200238827096No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT50870.35153744941509474No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG48470.3349522345812786No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT43610.30136717454280093No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC40420.2793226598261869No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG35690.24663596559120765No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA31880.2203069370425245No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG28800.19902257800579376No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA26850.18554709095331814No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG26550.18347393909909113No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG26080.18022600119413548No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA25560.17663253798014197No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC24390.1685472457486566No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG23800.16447004710201013No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC23620.1632261559894739No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT23410.161774949691515No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT23400.16170584462970744No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT22890.1581814864775215No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG20580.14221821719997346No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA19840.1371044426262135No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC19350.1337182945976427No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG19180.13254350854691405No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC18920.1307467769399173No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC18300.12646226310784814No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC17880.1235598505119303No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT17830.12321432520289247No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC16170.1117428849428363No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG16070.11105183432476061No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT15450.10676732049269146No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG14770.10206817628977687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACAC307.201776E-5165.7883944
CGCTACC1550.0139.0483444
GCAATTA402.2651808E-4124.341344
TACACCT801.886292E-9124.341344
CGTGCGC6450.0110.52560444
TCACGTC505.50366E-499.4730444
ATGTTCT2550.091.0210744
TCGTGCG16200.084.845743
CCAGAAT3000.082.894244
ACGCTAC2700.075.1401143
TAAAACC1602.7848728E-972.53242544
ATTAGTC1156.601458E-772.0819144
ATCCTTG7600.072.0751943
AACGACA1300.070.227143
ACCACGA750.002752897866.3153644
CTAAAAC3750.066.27357543
AATCCCG4600.062.84816443
CAATGAA5250.061.8295843
TAATCCT7400.058.07239542
TGTGTTA10400.057.06197442
Produced by FastQC (version 0.11.5)