##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841203.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1447072
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.56378120784591	37.0	35.0	39.0	29.0	39.0
2	36.46537767298379	38.0	35.0	39.0	32.0	39.0
3	36.75765614979766	38.0	35.0	39.0	32.0	39.0
4	37.281162236571504	39.0	36.0	40.0	33.0	41.0
5	37.60540940602817	39.0	36.0	40.0	33.0	41.0
6	37.601956917140264	39.0	36.0	40.0	33.0	41.0
7	37.5087473187236	39.0	36.0	40.0	33.0	41.0
8	37.30167330996661	39.0	36.0	40.0	32.0	41.0
9	37.14519249906017	38.0	35.0	40.0	32.0	41.0
10	36.89484766480175	38.0	35.0	40.0	31.0	41.0
11	36.649281445567325	38.0	35.0	40.0	31.0	41.0
12	36.48733096901882	38.0	35.0	40.0	31.0	41.0
13	36.21525812122687	38.0	34.0	40.0	30.0	41.0
14	36.11269031534022	37.0	34.0	40.0	30.0	41.0
15	36.024076894584375	37.0	34.0	40.0	30.0	41.0
16	35.91380180115433	37.0	34.0	40.0	30.0	41.0
17	35.80988713761305	37.0	34.0	39.0	30.0	41.0
18	35.58507040423697	36.0	34.0	39.0	30.0	41.0
19	35.416019382587734	36.0	33.0	39.0	30.0	40.0
20	35.272165448574775	36.0	33.0	39.0	29.0	40.0
21	35.006654817452066	35.0	33.0	39.0	29.0	40.0
22	34.957674531744104	35.0	33.0	39.0	29.0	40.0
23	34.9080660810243	35.0	33.0	39.0	29.0	40.0
24	34.76408084739391	35.0	33.0	38.0	29.0	40.0
25	34.683553910110334	35.0	33.0	38.0	29.0	40.0
26	34.57397944938721	35.0	33.0	38.0	29.0	40.0
27	34.522280767675674	35.0	33.0	38.0	29.0	40.0
28	34.49151194418805	35.0	33.0	37.0	29.0	40.0
29	34.474023802985464	35.0	33.0	37.0	29.0	40.0
30	34.43594430553167	35.0	33.0	37.0	29.0	40.0
31	34.32889039860795	35.0	33.0	37.0	29.0	40.0
32	34.27577725319763	35.0	33.0	37.0	29.0	39.0
33	34.18807111584347	35.0	33.0	36.0	29.0	39.0
34	34.12868588957807	35.0	33.0	36.0	29.0	39.0
35	34.03054848558035	35.0	33.0	36.0	29.0	39.0
36	33.93690053789731	35.0	33.0	36.0	29.0	39.0
37	33.87326708737956	35.0	33.0	35.0	29.0	39.0
38	33.823515518087476	35.0	33.0	35.0	29.0	38.0
39	33.695706626752816	35.0	33.0	35.0	29.0	38.0
40	33.57835367371744	35.0	33.0	35.0	29.0	37.0
41	33.492346198640924	35.0	33.0	35.0	29.0	37.0
42	33.39995009478641	34.0	33.0	35.0	29.0	37.0
43	33.36017460767615	34.0	33.0	35.0	29.0	37.0
44	33.341033801154985	34.0	33.0	35.0	29.0	37.0
45	33.33104288196735	34.0	33.0	35.0	29.0	36.0
46	33.25022428817545	34.0	33.0	35.0	29.0	36.0
47	33.224132213943854	34.0	33.0	35.0	29.0	36.0
48	33.286157129178314	34.0	33.0	35.0	29.0	36.0
49	33.23220735112922	34.0	32.0	35.0	29.0	36.0
50	33.94365896077333	35.0	33.0	36.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	6.0
22	32.0
23	114.0
24	356.0
25	1095.0
26	3034.0
27	7284.0
28	15152.0
29	29289.0
30	50774.0
31	79523.0
32	114714.0
33	157349.0
34	206250.0
35	231134.0
36	222360.0
37	163581.0
38	103582.0
39	50222.0
40	11219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.92266314322991	19.648296698436567	34.42904015833352
2	29.813098449835252	19.471318635147387	27.61403717299485	23.101545742022513
3	28.486004842882735	15.68664171513235	26.9798600207868	28.847493421198116
4	19.577118484774772	16.81098107074147	31.87747396121271	31.734426483271044
5	23.359031202317507	17.502239004002565	27.762682160942926	31.376047632737002
6	27.008745936622365	17.880589217398995	27.033485548749475	28.077179297229165
7	32.1148498485217	18.320097410495123	21.951568408482785	27.613484332500388
8	27.15635434864333	15.792165144512504	28.093626301939363	28.957854204904798
9	27.81748247495632	15.391563101214038	25.970442382963665	30.82051204086597
10	27.48246113531324	18.21346830012605	27.39379934101413	26.910271223546584
11	32.043948055107144	15.435237500276418	25.962771721103028	26.558042723513413
12	32.590292673757766	16.289790694588795	22.280992238119456	28.838924393533976
13	27.322206496981487	17.27571261139736	27.2410080493576	28.16107284226355
14	28.63112547267862	17.64749784392207	21.499344884014064	32.22203179938524
15	26.158684571327477	19.370770770217376	22.643448287300146	31.827096371154994
16	28.30771378341921	20.125743570465048	25.790769222263993	25.77577342385175
17	28.89794011631764	18.96954678136264	18.225976316313215	33.906536786006505
18	27.07736726299728	19.048188341699653	22.542762212246522	31.331682183056547
19	28.301632537980144	16.55840206983481	26.803918533424735	28.336046858760312
20	28.541081577143363	16.793013754671502	24.698494615333583	29.96741005285155
21	30.671314212423432	19.780563786736252	21.070409765374496	28.477712235465823
22	30.051303597885937	19.07728157272064	24.182625328940095	26.688789500453332
23	29.758505451007277	21.50452776364963	22.18749308949382	26.549473695849272
24	32.961248645540785	20.216824041927424	20.307973618451605	26.513953694080183
25	30.419819136127284	21.93378014718163	22.656201411439806	24.990199305251284
26	31.07182818743369	20.99549673405426	21.169378922510038	26.76329615600201
27	29.217522870111484	21.16394330298272	23.06777382341974	26.550760003486058
28	30.032843720614032	20.801456064397076	24.143273965415585	25.022426249573304
29	32.81560370993836	19.28298222752181	23.915014936049232	23.9863991264906
30	29.253413652847414	22.756264672202597	22.04200310753025	25.948318567419737
31	28.509681816796583	20.275510515052883	26.56172302221641	24.65308464593413
32	28.534800623693446	19.53259008363435	25.860992597016896	26.071616695655308
33	27.11293428314226	18.28822044303687	26.649329847768172	27.949515426052702
34	26.424863320993403	17.485029664860622	29.8180115962637	26.272095417882273
35	30.645401006929607	18.826595698565157	24.04880450331618	26.479198791189056
36	28.448234718826406	19.15876772616137	22.766239608801957	29.62675794621027
37	26.338612785079334	19.731345217366574	27.617578931069026	26.312463066485066
38	30.579669091097696	19.80059287512518	21.956408118319683	27.663329915457442
39	28.387327782813603	19.7346944042116	25.52209509001584	26.355882722958956
40	27.898800637026383	18.884110011942205	23.865633471475437	29.351455879555978
41	26.892000353780116	21.499911968265593	23.16623284517739	28.441854832776905
42	26.629455463783554	19.731231955413545	26.93198488776351	26.707327693039396
43	24.630601684942	23.46555990102961	25.284828559444218	26.619009854584174
44	24.04924831295675	22.07922966612262	24.418567595850327	29.452954425070306
45	27.598118830305946	19.391197153258716	26.739396142081954	26.271287874353384
46	25.07354150135466	21.065484998606017	24.573226961761993	29.287746538277325
47	29.35257540766651	20.028992226679303	23.609395281231446	27.009037084422744
48	23.59680314104384	22.67056089381174	25.181880295820118	28.550755669324303
49	28.293656817852842	21.91481699847131	22.379072829912904	27.412453353762945
50	25.228374482148947	24.105136042433454	24.466837309543482	26.19965216587412
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	421.0
1	439.5
2	514.5
3	559.5
4	532.5
5	422.0
6	284.5
7	258.5
8	293.0
9	338.0
10	386.5
11	414.0
12	423.5
13	443.5
14	457.5
15	479.5
16	513.5
17	588.0
18	734.0
19	902.0
20	1015.0
21	1238.0
22	1589.0
23	2066.0
24	2613.5
25	3155.0
26	3785.0
27	4561.0
28	5460.5
29	6516.5
30	7844.5
31	9348.5
32	10935.0
33	12261.5
34	13706.0
35	15968.0
36	18776.0
37	21061.5
38	23081.0
39	27002.0
40	31777.0
41	38674.5
42	49781.0
43	56086.0
44	56736.0
45	57952.5
46	62580.5
47	68499.5
48	75722.5
49	86012.5
50	98061.0
51	107817.5
52	117849.5
53	128665.0
54	128361.5
55	125507.0
56	126309.5
57	129288.5
58	130634.0
59	123986.0
60	119578.0
61	119616.0
62	117989.0
63	111353.0
64	98937.0
65	89332.0
66	82616.5
67	76509.0
68	68356.0
69	58903.0
70	52058.5
71	46454.5
72	41838.0
73	37287.0
74	31725.0
75	26672.0
76	23316.5
77	18695.0
78	14442.0
79	11568.0
80	8675.5
81	6372.5
82	4807.5
83	3540.0
84	2685.0
85	2052.0
86	1496.0
87	1070.0
88	756.5
89	549.0
90	398.0
91	246.5
92	162.5
93	140.0
94	91.0
95	36.5
96	20.0
97	18.0
98	9.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	16051.0
25	16270.0
26	14889.0
27	14209.0
28	12800.0
29	12287.0
30	15209.0
31	12651.0
32	14125.0
33	13333.0
34	13400.0
35	13723.0
36	12337.0
37	12573.0
38	13211.0
39	14937.0
40	15276.0
41	15527.0
42	16538.0
43	16671.0
44	16448.0
45	25511.0
46	96753.0
47	191027.0
48	264818.0
49	393427.0
50	173071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.111971881091744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.476912837026816	15.186945346186539
2	12.96816261083927	6.5131226966564215
3	6.667421290874867	5.022962879275263
4	4.303648067927486	4.322923570716394
5	3.0982545271071604	3.890164028259011
6	2.191336951935502	3.3017275151400973
7	1.674087215431025	2.9427741756279353
8	1.3278587196477742	2.6676120663885916
9	1.0506714493069724	2.3745988702136342
>10	5.675461574995693	24.579591530781325
>50	0.3144466327185194	5.441615684049983
>100	0.21355835769317316	10.527987056843497
>500	0.021728321257661044	3.59741106319458
>1k	0.01520982488036273	7.31096570906689
>5k	9.312137681854732E-4	1.4733086283166292
>10k+	3.1040458939515775E-4	0.8462891792832994
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	10857	0.7502736560447579	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	8598	0.5941653214214635	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	5216	0.36045200238827096	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	5087	0.35153744941509474	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	4847	0.3349522345812786	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	4361	0.30136717454280093	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	4042	0.2793226598261869	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	3569	0.24663596559120765	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	3188	0.2203069370425245	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	2880	0.19902257800579376	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	2685	0.18554709095331814	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	2655	0.18347393909909113	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	2608	0.18022600119413548	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	2556	0.17663253798014197	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	2439	0.1685472457486566	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	2380	0.16447004710201013	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	2362	0.1632261559894739	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	2341	0.161774949691515	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	2340	0.16170584462970744	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	2289	0.1581814864775215	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	2058	0.14221821719997346	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA	1984	0.1371044426262135	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	1935	0.1337182945976427	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	1918	0.13254350854691405	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	1892	0.1307467769399173	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	1830	0.12646226310784814	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	1788	0.1235598505119303	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	1783	0.12321432520289247	No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC	1617	0.1117428849428363	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	1607	0.11105183432476061	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT	1545	0.10676732049269146	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	1477	0.10206817628977687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06074334932885164	0.0	0.0	0.0	0.0
2	0.15417339289268261	0.0	0.0	0.0	0.0
3	0.22293292939121204	0.0	0.0	0.0	0.0
4	0.2753836713031556	0.0	0.0	0.0	0.0
5	0.34476515335795316	0.0	0.0	0.0	0.0
6	0.4162197872669777	0.0	0.0	0.0	0.0
7	0.47841434289378826	0.0	0.0	0.0	0.0
8	0.5234708431923222	0.0	0.0	0.0	0.0
9	0.5621696778045598	0.0	0.0	0.0	0.0
10	0.6007303022931824	0.0	0.0	0.0	0.0
11	0.6354210433205811	0.0	0.0	0.0	0.0
12	0.6613354414984188	0.0	0.0	0.0	0.0
13	0.6819978549788815	0.0	0.0	0.0	0.0
14	0.7065992569823755	0.0	0.0	0.0	0.0
15	0.7342412817054024	0.0	0.0	0.0	0.0
16	0.7545581698768271	0.0	0.0	0.0	0.0
17	0.7742531124919838	0.0	0.0	0.0	0.0
18	0.7912529576966454	0.0	0.0	0.0	0.0
19	0.8236632316843944	0.0	0.0	0.0	0.0
20	0.8399029212091728	0.0	0.0	0.0	0.0
21	0.8618783308639791	0.0	0.0	0.0	0.0
22	0.8736261913712655	0.0	0.0	0.0	0.0
23	0.8849594215077066	0.0	0.0	0.0	0.0
24	0.8960162313969173	0.0	0.0	0.0	0.0
25	0.9062437805444373	0.0	0.0	0.0	0.0
26	0.9318817584750447	0.0	0.0	0.0	0.0
27	0.9414182570044891	0.0	0.0	0.0	0.0
28	0.9530279073881603	0.0	0.0	0.0	0.0
29	0.9647757678954468	0.0	0.0	0.0	0.0
30	0.9759707879082727	0.0	0.0	0.0	0.0
31	0.9874422281683288	0.0	0.0	0.0	0.0
32	0.9985681431193472	0.0	0.0	0.0	0.0
33	1.0074826960925234	0.0	0.0	0.0	0.0
34	1.0168809844983526	0.0	0.0	0.0	0.0
35	1.0282142146347937	0.0	0.0	0.0	0.0
36	1.0374051878552	0.0	0.0	0.0	0.0
37	1.0450758497158399	0.0	0.0	0.0	0.0
38	1.0502587293514076	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACAC	30	7.201776E-5	165.78839	44
CGCTACC	155	0.0	139.04834	44
GCAATTA	40	2.2651808E-4	124.3413	44
TACACCT	80	1.886292E-9	124.3413	44
CGTGCGC	645	0.0	110.525604	44
TCACGTC	50	5.50366E-4	99.47304	44
ATGTTCT	255	0.0	91.02107	44
TCGTGCG	1620	0.0	84.8457	43
CCAGAAT	300	0.0	82.8942	44
ACGCTAC	270	0.0	75.14011	43
TAAAACC	160	2.7848728E-9	72.532425	44
ATTAGTC	115	6.601458E-7	72.08191	44
ATCCTTG	760	0.0	72.07519	43
AACGACA	130	0.0	70.2271	43
ACCACGA	75	0.0027528978	66.31536	44
CTAAAAC	375	0.0	66.273575	43
AATCCCG	460	0.0	62.848164	43
CAATGAA	525	0.0	61.82958	43
TAATCCT	740	0.0	58.072395	42
TGTGTTA	1040	0.0	57.061974	42
>>END_MODULE