##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841202.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6219446
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.27347307139575	38.0	35.0	39.0	32.0	39.0
2	36.94595242084263	39.0	37.0	39.0	33.0	39.0
3	37.09402525562566	39.0	37.0	39.0	33.0	39.0
4	37.55544529207264	39.0	37.0	40.0	33.0	41.0
5	38.10507865169985	39.0	37.0	41.0	33.0	41.0
6	38.141695739459756	40.0	37.0	41.0	33.0	41.0
7	38.07016782523717	40.0	37.0	41.0	33.0	41.0
8	37.99874972786965	39.0	37.0	41.0	33.0	41.0
9	37.94632062083986	39.0	37.0	40.0	33.0	41.0
10	37.83442817897286	39.0	37.0	40.0	33.0	41.0
11	37.72866924160126	39.0	36.0	40.0	33.0	41.0
12	37.58711611934568	39.0	36.0	40.0	32.0	41.0
13	37.485824460892495	39.0	36.0	40.0	32.0	41.0
14	37.4093158779737	39.0	36.0	40.0	32.0	41.0
15	37.37784297829742	39.0	36.0	40.0	32.0	41.0
16	37.30348458689086	39.0	36.0	40.0	32.0	41.0
17	37.25408340228374	39.0	36.0	40.0	32.0	41.0
18	37.156569733059825	38.0	36.0	40.0	31.0	41.0
19	37.06131768006347	38.0	35.0	40.0	31.0	41.0
20	36.929006216952445	38.0	35.0	40.0	31.0	41.0
21	36.76090780432855	38.0	35.0	40.0	31.0	41.0
22	36.76638546262802	38.0	35.0	40.0	31.0	41.0
23	36.76079991690578	38.0	35.0	40.0	31.0	41.0
24	36.748311827130586	38.0	35.0	40.0	31.0	41.0
25	36.725146402127805	38.0	35.0	40.0	31.0	41.0
26	36.74168603223248	38.0	35.0	40.0	31.0	41.0
27	36.77676919631797	38.0	35.0	40.0	31.0	41.0
28	36.74901779867352	38.0	35.0	40.0	31.0	41.0
29	36.78382939012067	38.0	35.0	40.0	31.0	41.0
30	36.797601212119154	38.0	35.0	40.0	31.0	41.0
31	36.71983574347029	38.0	35.0	40.0	31.0	41.0
32	36.698675690723576	38.0	35.0	40.0	31.0	41.0
33	36.634792202245876	38.0	35.0	40.0	31.0	41.0
34	36.55952542709379	38.0	35.0	40.0	31.0	41.0
35	36.497063827958605	38.0	35.0	40.0	31.0	41.0
36	36.38595678334381	38.0	34.0	40.0	31.0	41.0
37	36.33808735169477	38.0	34.0	40.0	31.0	41.0
38	36.258899925294614	37.0	34.0	40.0	31.0	41.0
39	36.13658112078229	37.0	34.0	40.0	31.0	41.0
40	36.02275809884116	37.0	34.0	40.0	30.0	41.0
41	35.86708321880511	37.0	34.0	40.0	30.0	41.0
42	35.72772199971772	36.0	34.0	40.0	30.0	41.0
43	35.602028076797716	36.0	34.0	39.0	30.0	41.0
44	35.509204934363964	36.0	34.0	39.0	30.0	41.0
45	35.45366168518531	36.0	34.0	39.0	30.0	41.0
46	35.383993313150434	36.0	34.0	39.0	30.0	41.0
47	35.35006128807555	36.0	33.0	39.0	30.0	40.0
48	35.3985922126566	36.0	34.0	39.0	30.0	40.0
49	35.40807783350135	36.0	34.0	39.0	30.0	40.0
50	35.86289202424502	37.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	7.0
21	24.0
22	69.0
23	206.0
24	548.0
25	1427.0
26	3480.0
27	8782.0
28	21491.0
29	48291.0
30	96085.0
31	173045.0
32	277412.0
33	408446.0
34	555529.0
35	650240.0
36	789737.0
37	892161.0
38	928416.0
39	898299.0
40	465747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.43227451448248	15.584056843648131	31.98366864186939
2	28.991086987490526	22.287354854435588	29.95300546061498	18.768552697458908
3	28.538924528004582	16.91200470266966	26.81020142308495	27.738869346240808
4	21.168830792967732	18.690989519002173	31.866359158034335	28.273820529995756
5	22.998254185340624	21.0699634661994	28.424911157681894	27.50687119077809
6	26.125671000278807	20.666583486696403	25.59567202609364	27.61207348693115
7	30.22825505680088	20.204822101518367	23.091204586389207	26.475718255291547
8	28.497489969363833	18.874639316749434	25.59691007848609	27.030960635400646
9	27.639792997639983	19.79100710899331	24.310686192950303	28.258513700416405
10	27.476482632054367	20.35427271174957	26.259895173943143	25.90934948225292
11	31.099232954189166	20.47968581124428	23.431202714839873	24.989878519726677
12	29.12378369391743	19.341304675689763	24.344113607546394	27.19079802284641
13	28.26644045144857	19.605508271958627	23.52174132551356	28.606309951079247
14	27.602715740276544	21.62945059736832	22.85856328682651	27.90927037552862
15	26.02540483509303	21.814949434403	23.607504591244943	28.552141139259028
16	28.024007282963787	22.344417814705682	23.14640242876938	26.485172473561153
17	28.779331792574453	21.421731131679575	20.529497321787183	29.269439753958792
18	28.760953949917727	20.584196727489875	21.45554443273565	29.199304889856748
19	27.605368709688932	20.778522717296685	24.235775983905963	27.38033258910842
20	29.831981819602582	19.612229127803342	23.932790798408732	26.622998254185344
21	29.722036335712215	22.381800565516606	22.12716373773484	25.768999361036336
22	30.33832916951124	21.352866477174974	22.579551297655772	25.729253055658013
23	29.354222224937722	22.58812119278791	22.9490054258852	25.108651156389172
24	32.243691801488424	22.274781387281116	20.82232083050484	24.659205980725616
25	29.290708514983955	22.700228840386632	22.730330440190006	25.278732204439407
26	30.0496416482247	22.695437552485412	22.495726568594435	24.759194230695453
27	30.370220787550238	21.915617868744715	22.544665080941765	25.169496262763282
28	29.786405231639222	22.347638993384315	22.682180188068386	25.18377558690808
29	30.939357602910178	21.575064564869738	23.257093716179956	24.228484116040125
30	29.721877698806036	22.61625332340201	23.08045220324374	24.581416774548213
31	28.242481533312386	22.00300664949904	25.269193534589242	24.485318282599337
32	27.855333908716638	20.437089648303434	25.637215695622345	26.07036074735758
33	27.837435835168606	20.156514736627617	26.16880130744131	25.837248120762467
34	26.90657442126028	20.0733377733534	28.61880409514409	24.401283710242225
35	28.080867522971754	20.610880048981777	24.92864222952324	26.379610198523228
36	27.370519035609224	22.631390666844787	23.531819856781283	26.466270440764706
37	26.152764410833022	22.934528840302875	26.310942185498632	24.60176456336547
38	28.252721612730134	22.263154788863655	23.715351472485345	25.76877212592087
39	26.53769552942991	23.08044900379152	24.84257353995435	25.539281926824213
40	27.39981826976914	22.54324867711631	23.54084197441849	26.516091078696057
41	26.212010870027232	22.39248279242952	24.891172211721834	26.504334125821416
42	26.134349029941145	23.91723011028412	25.10200124004133	24.84641961973341
43	25.43468275405686	23.938394922963603	24.704619314368966	25.92230300861057
44	24.271372713121465	24.333217444107703	25.210645710119095	26.18476413265174
45	25.856141588313967	22.14277345905691	26.120873223205454	25.880211729423674
46	26.018323318861842	23.176249475809513	23.776039372326526	27.029387833002115
47	26.250346939106112	23.35253873028387	24.724873238980297	25.672241091629722
48	25.43457221120663	23.09302560207903	24.243392885117395	27.22900930159694
49	25.993616346900982	23.480366538859332	22.999490113394117	27.52652700084557
50	23.78744944549486	27.00842064167136	24.074832295405326	25.129297617428453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9979.0
1	9926.0
2	7486.0
3	5038.0
4	4833.0
5	3716.5
6	2284.0
7	1984.5
8	2259.0
9	2464.5
10	2603.0
11	2642.0
12	2594.5
13	2633.0
14	2872.5
15	3276.0
16	3620.0
17	4151.5
18	4935.5
19	6265.5
20	7752.5
21	9650.5
22	11933.0
23	15179.5
24	18688.0
25	22057.5
26	26469.5
27	32420.0
28	39023.5
29	46100.0
30	53636.5
31	64033.5
32	76310.0
33	86166.5
34	98273.5
35	113764.5
36	126058.0
37	138555.0
38	152483.0
39	170482.0
40	193112.0
41	223300.0
42	261133.5
43	291861.0
44	311517.5
45	321654.0
46	339550.5
47	364374.5
48	400494.5
49	440781.0
50	480089.0
51	515266.0
52	533246.5
53	535825.0
54	518982.0
55	497474.0
56	483220.5
57	476078.5
58	462026.0
59	435989.5
60	413620.5
61	397949.5
62	377696.5
63	353191.0
64	323022.0
65	292231.0
66	270470.5
67	257014.0
68	237890.5
69	211725.0
70	187324.0
71	167263.5
72	153052.5
73	141999.0
74	126790.5
75	108844.0
76	94317.5
77	75382.5
78	58811.5
79	48159.5
80	37188.5
81	27097.5
82	19633.0
83	14416.0
84	10698.5
85	8157.0
86	6147.0
87	4462.0
88	3257.0
89	2468.0
90	1739.5
91	1100.0
92	775.0
93	636.5
94	424.5
95	192.5
96	117.0
97	108.0
98	59.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	63627.0
25	56505.0
26	52868.0
27	57348.0
28	50497.0
29	44791.0
30	60739.0
31	47942.0
32	53017.0
33	50456.0
34	50174.0
35	54697.0
36	50092.0
37	53192.0
38	54304.0
39	62906.0
40	62836.0
41	64597.0
42	71361.0
43	72181.0
44	74959.0
45	95636.0
46	284946.0
47	684861.0
48	1114873.0
49	1411028.0
50	1419013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.0049840781998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.79849543359416	12.498499876819439
2	12.313898553021465	5.419342831996097
3	6.856074609714156	4.526034378771298
4	4.58187958271067	4.032967490631082
5	3.443042344256212	3.7882045982962826
6	2.6740195730609093	3.5305054877999966
7	2.093659055552569	3.224965392782918
8	1.660767940924576	2.9236137678103966
9	1.38429368328562	2.741522441422737
>10	7.588387019787579	28.23262534374521
>50	0.33940675484560123	5.176274163180346
>100	0.22276742708366407	9.591466076946235
>500	0.023954257530523146	3.670120363456074
>1k	0.017450115652094523	7.26693179459638
>5k	0.0014277367351709918	2.068708615168206
>10k+	4.7591224505699726E-4	1.3082173765774352
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	16715	0.26875384077617204	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	13984	0.22484317735052287	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	12977	0.20865202463370533	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	10701	0.17205712534524778	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	10368	0.16670295071297347	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	10207	0.1641142957105826	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	9659	0.15530322154095397	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	8282	0.1331629858993872	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	7638	0.12280836588982363	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	7583	0.1219240427523609	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	7463	0.11999461045244222	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	7421	0.11931930914747069	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	7350	0.1181777283700188	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	6767	0.10880390311291392	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	6326	0.10171323941071278	Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	6292	0.10116656692573582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.23826881043745696	0.0	0.0	0.0	0.0
2	0.5936059256724795	0.0	0.0	0.0	0.0
3	0.8064223083535093	0.0	0.0	0.0	0.0
4	0.962159652161945	0.0	0.0	0.0	0.0
5	1.1784007771753304	0.0	0.0	0.0	0.0
6	1.3796244874543488	0.0	0.0	0.0	0.0
7	1.5799477959934052	0.0	0.0	0.0	0.0
8	1.7351223887143645	0.0	0.0	0.0	0.0
9	1.8707775580011468	0.0	0.0	0.0	0.0
10	1.993618081095969	0.0	0.0	0.0	0.0
11	2.112840918628444	0.0	0.0	0.0	0.0
12	2.2100682279418455	0.0	0.0	0.0	0.0
13	2.2827435112387824	0.0	0.0	0.0	0.0
14	2.362364750815426	0.0	0.0	0.0	0.0
15	2.4486746890317885	0.0	0.0	0.0	0.0
16	2.5102557366041927	0.0	0.0	0.0	0.0
17	2.5713222688966186	0.0	0.0	0.0	0.0
18	2.6268899191342765	0.0	0.0	0.0	0.0
19	2.754521865773897	0.0	0.0	0.0	0.0
20	2.7943967999722163	0.0	0.0	0.0	0.0
21	2.859692004721964	0.0	0.0	0.0	0.0
22	2.8856750263608686	0.0	0.0	0.0	0.0
23	2.909696458494856	0.0	0.0	2.25100434990512E-4	0.0
24	2.9305021701289795	0.0	0.0	2.25100434990512E-4	0.0
25	2.955038117542945	0.0	0.0	2.25100434990512E-4	1.6078602499322288E-5
26	3.0401743177768568	0.0	0.0	2.25100434990512E-4	1.6078602499322288E-5
27	3.059147068726057	0.0	0.0	2.25100434990512E-4	1.6078602499322288E-5
28	3.079164928837713	0.0	0.0	2.25100434990512E-4	1.6078602499322288E-5
29	3.0959992256545035	0.0	0.0	2.25100434990512E-4	1.6078602499322288E-5
30	3.1115472342713484	0.0	0.0	2.25100434990512E-4	1.6078602499322288E-5
31	3.1269183782607004	0.0	0.0	2.25100434990512E-4	1.6078602499322288E-5
32	3.14211265762256	1.6078602499322288E-5	0.0	2.25100434990512E-4	1.6078602499322288E-5
33	3.1571943867669243	1.6078602499322288E-5	0.0	2.25100434990512E-4	1.6078602499322288E-5
34	3.170603941251359	1.6078602499322288E-5	0.0	2.25100434990512E-4	1.6078602499322288E-5
35	3.1862484214831994	1.6078602499322288E-5	0.0	2.25100434990512E-4	1.6078602499322288E-5
36	3.2001724912476126	1.6078602499322288E-5	0.0	2.25100434990512E-4	1.6078602499322288E-5
37	3.2124404649545957	1.6078602499322288E-5	0.0	2.25100434990512E-4	1.6078602499322288E-5
38	3.2212193819192256	1.6078602499322288E-5	0.0	2.25100434990512E-4	1.6078602499322288E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	370	0.0	100.852516	44
TAAGCCA	1685	0.0	62.831863	44
ATGTTCT	1490	0.0	59.98891	44
AATCCCG	1685	0.0	58.994835	43
TTCAGGA	2540	0.0	57.739502	44
TCGTGCG	1835	0.0	55.365593	43
CGCTACC	830	0.0	51.231613	44
TTAAGCC	1205	0.0	47.607162	43
ACGCTAC	1435	0.0	47.605896	43
TTTGGAC	2665	0.0	46.83124	43
CACATGC	2225	0.0	46.412712	44
CGTGCGC	1615	0.0	46.21106	44
ATTAAGC	1235	0.0	45.47957	42
ACTTCTA	1155	0.0	44.32922	44
TACGCTA	1210	0.0	43.825974	42
AACGACA	970	0.0	43.791325	43
CTAATCC	5300	0.0	42.378635	41
CGGTCAC	825	0.0	42.075188	44
CTATCAT	875	0.0	41.654438	44
CTCGTGC	2055	0.0	41.53243	42
>>END_MODULE