FastQCFastQC Report
Tue 24 May 2016
ERR841201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299078
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG9620.3216552203772929No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC5970.19961347875804974No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT5870.19626986939861843No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA5330.1782143788576893No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA5080.169855355459111No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC4910.16417121954807776No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC4290.1434408415196036No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT4290.1434408415196036No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG4230.1414346759039448No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG3990.13341001344130962No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA3800.12705715565839013No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG3740.12505099004273135No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC3660.12237610255518627No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG3600.1203699369395275No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG3230.10799858230963161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTATC100.0054655587158.0938744
CCTGTTA352.2248272E-590.33935544
CTATCAT1400.084.69314644
GGTCGAG300.001368594179.04693644
GGACTTT404.3149623E-579.04693644
ACTTCTA1300.072.9664144
TCGTGCG1350.069.6735643
CGCTACC501.3030761E-463.23755644
AGGTCGA356.3516654E-661.07747743
CGTGCGC1055.0386006E-1060.22623444
ACATTGG400.004282056459.28520244
CCCTATC1050.058.8777742
AATCCCG454.2118154E-757.00564643
CTAAAAC1300.055.90937843
GCTAAGG450.006824557752.6979644
ATGTTCT1053.437526E-852.69795244
TTCAGGA2000.051.38051244
CCTATCA1250.051.3050843
GTACCAA250.00760057751.30507743
CGAAGAC508.716561E-751.30507743
Produced by FastQC (version 0.11.5)