##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841201.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	299078
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.870451855368835	38.0	35.0	39.0	30.0	39.0
2	36.59891399568006	38.0	35.0	39.0	32.0	39.0
3	36.849958873604876	38.0	35.0	39.0	33.0	39.0
4	37.22299868261791	39.0	36.0	39.0	33.0	41.0
5	37.75840750573429	39.0	36.0	40.0	33.0	41.0
6	37.73472137703208	39.0	36.0	40.0	33.0	41.0
7	37.60972054112974	39.0	36.0	40.0	33.0	41.0
8	37.50791432335377	39.0	36.0	40.0	33.0	41.0
9	37.37536027390848	39.0	36.0	40.0	32.0	41.0
10	37.10639364981711	38.0	35.0	40.0	32.0	41.0
11	36.95216632450398	38.0	35.0	40.0	31.0	41.0
12	36.85419522666328	38.0	35.0	40.0	31.0	41.0
13	36.65563832846281	38.0	35.0	40.0	31.0	41.0
14	36.49177137736644	38.0	35.0	40.0	31.0	41.0
15	36.38057630450919	38.0	34.0	40.0	31.0	41.0
16	36.28492567156394	38.0	34.0	40.0	31.0	41.0
17	36.181507834076726	37.0	34.0	40.0	31.0	41.0
18	35.99030018924829	37.0	34.0	40.0	30.0	41.0
19	35.86676385424538	37.0	34.0	40.0	30.0	41.0
20	35.641123720233516	36.0	34.0	39.0	30.0	41.0
21	35.416510074295	36.0	33.0	39.0	29.0	40.0
22	35.31500478136138	36.0	33.0	39.0	29.0	40.0
23	35.23911822333973	36.0	33.0	39.0	29.0	40.0
24	35.12753529179679	35.0	33.0	39.0	29.0	40.0
25	35.04380473476901	35.0	33.0	39.0	29.0	40.0
26	34.99980496817902	35.0	33.0	39.0	29.0	40.0
27	34.97107412299203	35.0	33.0	38.0	29.0	40.0
28	34.88366388527821	35.0	33.0	38.0	29.0	40.0
29	34.85667942164864	35.0	33.0	38.0	30.0	40.0
30	34.856562937324405	35.0	33.0	38.0	30.0	40.0
31	34.74093859229155	35.0	33.0	38.0	30.0	40.0
32	34.7101987428696	35.0	33.0	37.0	30.0	40.0
33	34.63424542912296	35.0	33.0	37.0	30.0	40.0
34	34.56972796042522	35.0	33.0	37.0	30.0	40.0
35	34.494118350671144	35.0	33.0	37.0	30.0	40.0
36	34.40515972741063	35.0	33.0	36.0	30.0	39.0
37	34.31817991352875	35.0	33.0	36.0	30.0	39.0
38	34.22787590196471	35.0	33.0	36.0	30.0	39.0
39	34.13863585179663	35.0	33.0	36.0	30.0	39.0
40	34.03229921921309	35.0	33.0	36.0	29.0	39.0
41	33.91458090225683	35.0	33.0	35.0	29.0	39.0
42	33.811297734433005	35.0	33.0	35.0	29.0	38.0
43	33.70529746159066	35.0	33.0	35.0	29.0	37.0
44	33.611560712696345	35.0	33.0	35.0	29.0	37.0
45	33.56897754277815	35.0	33.0	35.0	29.0	37.0
46	33.49069927807669	35.0	33.0	35.0	29.0	37.0
47	33.476630944317094	35.0	33.0	35.0	29.0	37.0
48	33.490139786405535	35.0	33.0	35.0	29.0	37.0
49	33.43913074868241	34.0	33.0	35.0	29.0	37.0
50	33.61125947795746	35.0	33.0	35.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	7.0
23	17.0
24	59.0
25	209.0
26	509.0
27	1289.0
28	2871.0
29	5277.0
30	9113.0
31	14468.0
32	21020.0
33	29490.0
34	39502.0
35	45512.0
36	44990.0
37	39414.0
38	26975.0
39	15307.0
40	3049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.47092731661974	14.069239462615105	33.45983322076515
2	25.46894121266024	21.64151157891921	31.872621857843104	21.01692535057744
3	25.96546720253579	17.68602170671196	27.90141702164653	28.447094069105717
4	19.610268893064685	18.97632055851651	32.28990430590014	29.123506242518676
5	20.726700058178803	21.436882686122015	28.67914055865025	29.15727669704893
6	24.815599943827362	20.532436354395845	26.750546680130267	27.90141702164653
7	28.12945117995974	20.203759554363746	23.91717210894817	27.749617156728345
8	26.959856626030664	18.633600599174795	25.20145246390574	29.2050903108888
9	27.5011869813226	19.297641417957855	25.390032031777665	27.811139568941883
10	26.63051110412668	19.341442700566407	26.862557593671216	27.165488601635694
11	28.537037160874423	20.509031088879823	24.473214345421596	26.480717404824162
12	28.93492667464675	18.678070603655232	24.203385070115488	28.183617651582534
13	27.147098750158822	19.28627314613579	24.236152441837916	29.33047566186747
14	26.273413624539415	22.316920669524336	23.37283250523275	28.036833200703498
15	25.323494205524984	22.81244357659206	23.05953630825403	28.804525909628925
16	26.71075772875303	24.09672393154963	22.25941058854212	26.933107751155216
17	28.04987327720528	22.780679287677465	19.3661854098262	29.803262025291062
18	26.305846635325903	21.393081403513463	22.552645129364247	29.748426831796387
19	27.42762757541511	20.599308541584467	23.448063715819952	28.52500016718047
20	28.108386440995325	19.395274811253252	24.588234507386034	27.90810424036539
21	28.236446679461547	23.529313423254134	21.68731902714342	26.5469208701409
22	29.542794856191364	21.517463671684308	22.266766529132866	26.672974942991463
23	28.366847444479365	23.046830592688195	23.113368418940876	25.472953543891556
24	30.395749603782292	23.049171119239798	21.449588401687855	25.10549087529006
25	28.620108555881913	24.294116054629868	22.017758767714778	25.06801662177344
26	29.388558133169095	24.424827208649834	21.90549510709642	24.28111955108465
27	30.057879414429056	23.470897787858462	20.73009660399278	25.741126193719705
28	30.520497015947555	22.69172711644094	22.027478440745245	24.760297426866256
29	30.255076106294652	23.186298042980994	22.188373669198835	24.370252181525515
30	29.4355599710854	23.81394951340149	22.219373493289464	24.531117022223647
31	27.684764883962863	22.89533079672713	24.72183125209234	24.698073067217667
32	27.147839988373363	22.42633433855321	25.141881335610215	25.28394433746321
33	26.791101194189793	21.13392981657773	25.97271052457741	26.102258464655065
34	27.012526335667285	20.763428876110357	27.031780531786087	25.19226425643627
35	26.047838162129427	21.568290303408627	26.077367891838954	26.306503642622985
36	26.764246417170412	21.926766133109947	23.815345604383165	27.493641845336477
37	25.032855265413406	23.53160771765423	26.24764299182904	25.18789402510333
38	27.167407726510458	22.0544794375125	23.619551671615614	27.158561164361434
39	26.58520296033983	22.854103083816756	24.855749442800672	25.704944513042737
40	25.80443819849809	22.305617818742814	24.523931962801726	27.36601201995737
41	25.05219372405198	22.929119732006065	24.50048819985235	27.518198344089605
42	25.260369634579494	23.50972247306002	25.02237473160356	26.20753316075693
43	24.81318493776895	23.953368359887687	24.325982633005417	26.907464069337948
44	23.533895930510457	23.75022663216364	25.243114502810236	27.472762934515664
45	25.64391708745244	22.038733173789744	26.160971962929086	26.156377775828727
46	24.55177756589253	22.916808554110197	24.673942654770823	27.857471225226455
47	24.871368140568716	23.421988758450226	25.75328700941581	25.95335609156525
48	24.64635814133147	22.836129409431148	24.677676977523515	27.83983547171387
49	26.25153418525738	23.17450003609848	22.51606382210671	28.057901956537435
50	22.9819499429645	25.60692478024559	24.64738643226196	26.763738844527946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	301.0
1	300.0
2	254.0
3	205.5
4	195.0
5	153.0
6	98.5
7	86.5
8	96.5
9	109.5
10	122.5
11	124.5
12	113.0
13	112.5
14	145.0
15	172.5
16	186.5
17	225.5
18	261.5
19	286.5
20	329.5
21	438.5
22	565.5
23	697.0
24	860.0
25	1089.0
26	1389.5
27	1593.5
28	1691.0
29	2023.0
30	2665.0
31	3552.5
32	4290.0
33	4620.0
34	5175.5
35	6008.0
36	6496.0
37	6861.0
38	7461.5
39	8420.0
40	9456.0
41	10880.5
42	12584.5
43	13567.5
44	14196.5
45	15202.5
46	16755.5
47	18150.0
48	20022.5
49	21865.0
50	23460.5
51	25425.0
52	26746.5
53	26679.0
54	26236.0
55	25813.0
56	24621.0
57	23237.5
58	21898.5
59	20233.0
60	19196.0
61	19038.5
62	18219.5
63	16660.0
64	15108.0
65	13973.5
66	13070.0
67	12332.0
68	11367.5
69	10014.5
70	8795.0
71	7836.0
72	7029.0
73	6294.0
74	5402.0
75	4571.5
76	3945.0
77	3144.0
78	2413.0
79	1982.0
80	1619.5
81	1168.5
82	820.0
83	610.0
84	426.0
85	303.0
86	215.0
87	153.0
88	94.5
89	61.0
90	55.5
91	48.0
92	39.5
93	34.0
94	18.5
95	3.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	3562.0
25	3256.0
26	3038.0
27	3034.0
28	2900.0
29	2461.0
30	3028.0
31	2569.0
32	2744.0
33	2415.0
34	2544.0
35	2513.0
36	2499.0
37	2527.0
38	2450.0
39	2795.0
40	2797.0
41	2781.0
42	3066.0
43	3415.0
44	3251.0
45	4505.0
46	12005.0
47	31345.0
48	53068.0
49	63995.0
50	74515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.76596071927725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.81058891637282	18.681748573950607
2	13.474027682753539	8.560308682016062
3	7.266985948107994	6.925283705254149
4	4.602915636019157	5.848641491517263
5	3.299826324930267	5.241107670908592
6	2.4367138571654126	4.644273400250102
7	1.8630598389558444	4.142731996335404
8	1.4346613336140204	3.64587164552391
9	1.1273090889953161	3.2229050615558483
>10	5.374453976106521	27.550338038906236
>50	0.199989474238198	4.3914965326770945
>100	0.10420504183990317	6.0796848982539675
>500	0.005262880901005211	1.0656083028507612
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	962	0.3216552203772929	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	597	0.19961347875804974	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	587	0.19626986939861843	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	533	0.1782143788576893	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	508	0.169855355459111	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	491	0.16417121954807776	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	429	0.1434408415196036	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	429	0.1434408415196036	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	423	0.1414346759039448	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	399	0.13341001344130962	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	380	0.12705715565839013	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	374	0.12505099004273135	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	366	0.12237610255518627	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	360	0.1203699369395275	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	323	0.10799858230963161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.09295234019219066	0.0	0.0	0.0	0.0
2	0.2591297253559272	0.0	0.0	0.0	0.0
3	0.35475695303566296	0.0	0.0	0.0	0.0
4	0.42196350116023246	0.0	0.0	0.0	0.0
5	0.5436708818435324	0.0	0.0	0.0	0.0
6	0.6392981095232682	0.0	0.0	0.0	0.0
7	0.72489450912471	0.0	0.0	0.0	0.0
8	0.7937728619289951	0.0	0.0	0.0	0.0
9	0.8526203866549863	0.0	0.0	0.0	0.0
10	0.9057837754699443	0.0	0.0	0.0	0.0
11	0.9626251345802768	0.0	0.0	0.0	0.0
12	1.0187977718187229	0.0	0.0	0.0	0.0
13	1.0515651435411497	0.0	0.0	0.0	0.0
14	1.0833294324557474	0.0	0.0	0.0	0.0
15	1.125793271320525	0.0	0.0	0.0	0.0
16	1.1575575602351227	0.0	0.0	0.0	0.0
17	1.1919967366372652	0.0	0.0	0.0	0.0
18	1.234460575502043	0.0	0.0	0.0	0.0
19	1.3498150984024235	0.0	0.0	0.0	0.0
20	1.3748921685981583	0.0	0.0	0.0	0.0
21	1.4267181136693439	0.0	0.0	0.0	0.0
22	1.4394238292351829	0.0	0.0	0.0	0.0
23	1.462829094751202	0.0	0.0	0.0	0.0
24	1.480550224356188	0.0	0.0	0.0	0.0
25	1.500611880512776	0.0	0.0	0.0	0.0
26	1.6042637706551468	0.0	0.0	0.0	0.0
27	1.619978734644474	0.0	0.0	0.0	0.0
28	1.6336875330181424	0.0	0.0	0.0	0.0
29	1.6497368579434128	0.0	0.0	0.0	0.0
30	1.6667892656765124	0.0	0.0	0.0	0.0
31	1.6764857328188634	0.0	0.0	0.0	0.0
32	1.688522726512816	0.0	0.0	0.0	0.0
33	1.703568968630257	0.0	0.0	0.0	0.0
34	1.7102561873491195	0.0	0.0	0.0	0.0
35	1.7239649857227881	0.0	0.0	0.0	0.0
36	1.7356676184807978	0.0	0.0	0.0	0.0
37	1.749376416854466	0.0	0.0	0.0	0.0
38	1.7530543871498405	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTATC	10	0.0054655587	158.09387	44
CCTGTTA	35	2.2248272E-5	90.339355	44
CTATCAT	140	0.0	84.693146	44
GGTCGAG	30	0.0013685941	79.046936	44
GGACTTT	40	4.3149623E-5	79.046936	44
ACTTCTA	130	0.0	72.96641	44
TCGTGCG	135	0.0	69.67356	43
CGCTACC	50	1.3030761E-4	63.237556	44
AGGTCGA	35	6.3516654E-6	61.077477	43
CGTGCGC	105	5.0386006E-10	60.226234	44
ACATTGG	40	0.0042820564	59.285202	44
CCCTATC	105	0.0	58.87777	42
AATCCCG	45	4.2118154E-7	57.005646	43
CTAAAAC	130	0.0	55.909378	43
GCTAAGG	45	0.0068245577	52.69796	44
ATGTTCT	105	3.437526E-8	52.697952	44
TTCAGGA	200	0.0	51.380512	44
CCTATCA	125	0.0	51.30508	43
GTACCAA	25	0.007600577	51.305077	43
CGAAGAC	50	8.716561E-7	51.305077	43
>>END_MODULE