FastQCFastQC Report
Tue 24 May 2016
ERR841200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4012829
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG176840.44068660787688685No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG174180.4340578679031676Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT150680.37549569144361744No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA148640.37041199612542675No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC125100.31175013936551993No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG115450.28770226690447065No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA94360.23514582854141058Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA90800.22627428180966594No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG80230.199933762440413No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA75980.18934273052751563Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA70120.17473956652526185Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC68570.17087695488644047No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG65990.16444757551343453Illumina Single End Adapter 1 (100% over 31bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA60980.15196261789376025No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC60750.15138945616670932No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC53740.13392048352920097No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG53610.13359652255304177No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA52660.13122911541957058No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA51840.12918566926225863No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG50530.1259211394255773No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG49100.12235756868782598No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA48960.1220086876365776No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC48710.12138568575934833No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT43430.10822788611226644No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA43390.10812820581190974No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA41750.10404131349728582Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA40700.10142470561292295Illumina Single End Adapter 1 (100% over 32bp)
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG40290.10040298253426697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA21800.0103.4742944
CTATACG2950.079.4449244
TCGTGCG31000.072.4868143
TTAAGCC20050.070.40288543
CGTGCGC15000.063.96143744
ATTAAGC26950.050.55055642
AATCCCG6000.049.10473643
TACGCTA5650.048.3329542
CTCGTGC37450.047.356342
CTAATCC25500.045.15178341
TGTGTTA20550.044.64379542
ATTAGTC3900.043.19181444
CCTTAGC29550.042.85828443
ACGCTAC8700.042.4580343
ACCTTAG21550.042.42977542
AACGACA3150.041.8803743
GATTAAG29950.041.31063541
CAATGAA16650.040.67300843
ATAAGAT14900.040.43282343
AACCTTA26950.040.03363441
Produced by FastQC (version 0.11.5)