##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841200.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4012829
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.36658576779624	37.0	35.0	39.0	30.0	39.0
2	36.16333015934644	37.0	35.0	39.0	32.0	39.0
3	36.39845679943003	37.0	35.0	39.0	32.0	39.0
4	36.80614773268435	38.0	35.0	39.0	33.0	40.0
5	37.46004252860015	39.0	35.0	40.0	33.0	41.0
6	37.52495757980218	39.0	35.0	40.0	33.0	41.0
7	37.499826930078505	39.0	35.0	40.0	33.0	41.0
8	37.38337840959582	39.0	35.0	40.0	33.0	41.0
9	37.33603500174067	38.0	35.0	40.0	33.0	41.0
10	37.25003482580494	39.0	35.0	40.0	32.0	41.0
11	37.13044388385351	38.0	35.0	40.0	32.0	41.0
12	37.06791517904202	38.0	35.0	40.0	32.0	41.0
13	36.978049401058456	38.0	35.0	40.0	32.0	41.0
14	36.90731152511109	38.0	35.0	40.0	31.0	41.0
15	36.83720163505597	38.0	35.0	40.0	31.0	41.0
16	36.817836244704175	38.0	35.0	40.0	31.0	41.0
17	36.80774062388405	38.0	35.0	40.0	31.0	41.0
18	36.695573123100935	38.0	35.0	40.0	31.0	41.0
19	36.59449106852049	38.0	35.0	40.0	31.0	41.0
20	36.498899155682935	38.0	35.0	40.0	31.0	41.0
21	36.306872533068315	38.0	34.0	40.0	31.0	41.0
22	36.249548635139945	38.0	34.0	40.0	30.0	41.0
23	36.31900312722022	38.0	34.0	40.0	31.0	41.0
24	36.319603451829124	38.0	34.0	40.0	31.0	41.0
25	36.29949989797687	38.0	34.0	40.0	31.0	41.0
26	36.303171141829914	38.0	34.0	40.0	31.0	41.0
27	36.34974142541273	38.0	34.0	40.0	31.0	41.0
28	36.34836682398729	38.0	34.0	40.0	31.0	41.0
29	36.38963946443517	38.0	35.0	40.0	31.0	41.0
30	36.422980435191604	38.0	35.0	40.0	31.0	41.0
31	36.37935714229081	38.0	35.0	40.0	31.0	41.0
32	36.31618066933396	37.0	34.0	40.0	31.0	41.0
33	36.217696050091526	37.0	34.0	40.0	31.0	41.0
34	36.20126648072573	37.0	34.0	40.0	31.0	41.0
35	36.131927942373736	37.0	34.0	40.0	31.0	41.0
36	36.064497210731325	37.0	34.0	40.0	31.0	41.0
37	35.98754506498068	37.0	34.0	40.0	31.0	41.0
38	35.89317127687871	36.0	34.0	40.0	31.0	41.0
39	35.767411654951665	36.0	34.0	40.0	30.0	41.0
40	35.65954580295227	36.0	34.0	40.0	30.0	41.0
41	35.54200887974354	35.0	34.0	39.0	30.0	41.0
42	35.43176323271379	35.0	34.0	39.0	30.0	41.0
43	35.345121106994114	35.0	34.0	39.0	30.0	41.0
44	35.24379411198202	35.0	34.0	39.0	30.0	40.0
45	35.188833088509035	35.0	34.0	39.0	30.0	40.0
46	35.1204152029927	35.0	33.0	39.0	30.0	40.0
47	35.11977172833064	35.0	33.0	39.0	30.0	40.0
48	35.17367114012482	35.0	33.0	39.0	30.0	40.0
49	35.25037114523482	35.0	33.0	39.0	30.0	40.0
50	35.79497671599019	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	27.0
22	166.0
23	501.0
24	1312.0
25	3189.0
26	6994.0
27	14266.0
28	28145.0
29	52303.0
30	91783.0
31	149529.0
32	228238.0
33	335520.0
34	489065.0
35	354483.0
36	426208.0
37	501265.0
38	533091.0
39	519077.0
40	277663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	57.12329132390141	13.864732337211477	29.011976338887102
2	28.19940745045453	22.74537489636364	30.11311969685227	18.94209795632956
3	28.629328585892893	17.409189377369433	24.58365407546646	29.377827961271212
4	23.238617942603586	19.51972037682144	29.606046008937838	27.635615671637144
5	23.465465386140302	21.997598203162905	26.4311038422021	28.105832568494694
6	25.76361963093867	24.113810979735245	24.649193872950978	25.473375516375107
7	31.208930158748355	23.21524291216994	21.128086943151576	24.447739985930124
8	28.85821947558692	20.37415499140382	25.491243210214044	25.276382322795214
9	26.875054979915664	23.153490966098982	24.49862677926221	25.47282727472314
10	28.882715909399575	20.535587237831464	25.326820554775697	25.254876297993263
11	28.235292358582935	21.390096612639116	24.742394953784476	25.632216074993476
12	29.04541907965677	19.73834419557873	25.27919829128029	25.937038433484204
13	26.700190813014956	19.538883914565012	24.60914731228268	29.15177796013735
14	26.793342053698275	21.995828877831574	23.1771401173586	28.033688951111547
15	24.530923196577774	22.878099216288557	25.130973684649906	27.46000390248376
16	26.064654138015847	26.712875131235347	22.728055444176665	24.494415286572142
17	28.212739690627238	22.836083969688218	20.97024318753677	27.980933152147774
18	27.96924563693095	21.779946267334093	22.371872810927155	27.8789352848078
19	26.149706354295187	21.353115221206785	24.073839179292214	28.423339245205813
20	28.616145866170722	19.524629631614008	23.983703267694686	27.875521234520583
21	29.929035102168573	22.322356621724975	22.875931169755802	24.872677106350658
22	27.414549685521113	21.94347180006923	24.01071662909135	26.631261885318313
23	27.92299397756545	23.326984528869783	22.843784272890773	25.906237220673994
24	29.51037784067051	21.390370733465094	23.033500804544623	26.065750621319772
25	26.852009100815206	23.862668567743732	24.1210349044895	25.164287426951564
26	27.682413076875616	22.761878865291656	23.698599983395702	25.857108074437026
27	27.894285605302105	22.198988639409475	24.17496389735046	25.73176185793796
28	28.46083428980349	22.01107595018404	23.628865830342587	25.899223929669883
29	29.317863287511354	21.237742423008587	24.37859083166673	25.06580345781333
30	26.559385925055985	23.899692427984704	25.228713778796553	24.31220786816276
31	27.275201983228193	22.576954486710203	26.31739051147941	23.830453018582194
32	27.216659656598218	21.847116141409394	25.747985861087557	25.188238340904828
33	25.993782303949907	22.008824019435632	26.630916579559695	25.366477097054762
34	24.78018657872767	22.84522338669255	27.938429619502486	24.436160415077296
35	27.23226696091437	22.772733596999114	24.993559887535664	25.001439554550853
36	26.245957773238942	23.410428597115594	23.39144564892457	26.952167980720898
37	25.47852476290832	24.24949771689498	25.680449596066033	24.591527924130663
38	27.327695081333385	24.457358696667324	22.61165581284705	25.60329040915224
39	26.09829571435465	24.92825088408238	24.6016746438969	24.371778757666068
40	26.44177767453045	24.16645488348723	23.181859598959804	26.209907843022524
41	25.79048713522672	24.89561050891573	23.420471077120204	25.893431278737346
42	25.327819540786788	25.116930119131613	24.82592661406579	24.72932372601581
43	24.940997061128467	25.094251840918936	24.872406239282686	25.09234485866991
44	23.627920300620893	24.226058227509558	25.1453786162164	27.000642855653144
45	26.133114541381318	23.218673868222844	26.24514381703834	24.4030677733575
46	24.89038679003234	24.75886994339581	24.722157991263884	25.628585275307962
47	28.029271836357815	24.086733557272716	23.924239181238015	23.959755425131455
48	24.63567671235403	24.794537494218005	23.887744015539543	26.682041777888422
49	26.50918217427169	24.48170013879871	22.322060214163837	26.687057472765762
50	23.64378679746126	28.803743811597	23.588952265483638	23.963517125458104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6902.0
1	6861.5
2	4612.5
3	2394.5
4	2345.0
5	1897.5
6	1329.0
7	1302.5
8	1547.0
9	1730.0
10	1818.0
11	1821.5
12	1777.5
13	1794.5
14	1944.0
15	2203.0
16	2468.5
17	2916.5
18	3473.5
19	4388.5
20	5555.0
21	7244.0
22	9253.5
23	11634.0
24	14129.5
25	16798.0
26	20086.5
27	24940.0
28	30178.0
29	35352.0
30	41302.5
31	48987.0
32	57327.5
33	65625.5
34	75112.0
35	86413.5
36	98078.0
37	107979.0
38	116369.0
39	130735.5
40	149351.5
41	177832.5
42	218529.5
43	256017.5
44	269091.5
45	249749.5
46	237281.5
47	240740.5
48	256165.5
49	280454.0
50	308039.5
51	327276.5
52	341296.0
53	351160.5
54	337185.0
55	308410.5
56	279802.5
57	264344.0
58	256381.5
59	251601.5
60	252861.0
61	249224.5
62	242583.0
63	230874.5
64	200621.5
65	171731.5
66	154382.5
67	142282.5
68	129897.5
69	115087.0
70	100722.5
71	89511.5
72	84086.5
73	77958.5
74	65255.5
75	52842.0
76	44338.5
77	34735.5
78	27474.5
79	22900.5
80	17563.5
81	12467.0
82	9116.5
83	7019.5
84	5531.5
85	4333.0
86	3109.5
87	2081.0
88	1421.5
89	1055.0
90	776.0
91	529.5
92	395.0
93	345.5
94	223.0
95	84.5
96	50.0
97	45.0
98	27.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	38240.0
25	35850.0
26	32516.0
27	32605.0
28	29975.0
29	27301.0
30	32612.0
31	34361.0
32	46068.0
33	34394.0
34	39312.0
35	36897.0
36	33948.0
37	36027.0
38	41147.0
39	46459.0
40	44929.0
41	42462.0
42	44077.0
43	43197.0
44	45221.0
45	58221.0
46	180907.0
47	373195.0
48	780845.0
49	733333.0
50	1088730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.172007431688755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.21713897638938	13.25835969580863
2	12.422884140972517	5.757263272752471
3	6.7914465860660895	4.721143522927219
4	4.694289226906756	4.351044194095192
5	3.453331916420078	4.001031641568701
6	2.6568629431595747	3.693890871832327
7	2.0592450594795455	3.340178927534986
8	1.687703667883704	3.128598554775165
9	1.3534924289244372	2.822682295959437
>10	7.173646249445276	27.805694700368445
>50	0.27642831240222443	4.406404620841299
>100	0.17616499986812945	8.022773761649308
>500	0.021737786274716345	3.490370715938375
>1k	0.013277674859096561	6.242305290951082
>5k	0.001645021663958866	2.532590098545813
>10k+	7.050092845537998E-4	2.425667834451578
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	17684	0.44068660787688685	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	17418	0.4340578679031676	Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	15068	0.37549569144361744	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	14864	0.37041199612542675	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	12510	0.31175013936551993	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	11545	0.28770226690447065	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	9436	0.23514582854141058	Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	9080	0.22627428180966594	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	8023	0.199933762440413	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	7598	0.18934273052751563	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	7012	0.17473956652526185	Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	6857	0.17087695488644047	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	6599	0.16444757551343453	Illumina Single End Adapter 1 (100% over 31bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	6098	0.15196261789376025	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	6075	0.15138945616670932	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	5374	0.13392048352920097	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	5361	0.13359652255304177	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	5266	0.13122911541957058	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5184	0.12918566926225863	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	5053	0.1259211394255773	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	4910	0.12235756868782598	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4896	0.1220086876365776	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	4871	0.12138568575934833	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	4343	0.10822788611226644	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	4339	0.10812820581190974	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA	4175	0.10404131349728582	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	4070	0.10142470561292295	Illumina Single End Adapter 1 (100% over 32bp)
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	4029	0.10040298253426697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.4747523505237826	0.0	0.0	0.0	0.0
2	0.8264992104074208	0.0	0.0	0.0	0.0
3	1.0114809277943315	0.0	0.0	0.0	0.0
4	1.1566902053389267	0.0	0.0	0.0	0.0
5	1.396346567471477	0.0	0.0	0.0	0.0
6	1.5528944791816446	0.0	0.0	0.0	0.0
7	1.698004076425883	0.0	0.0	0.0	0.0
8	1.8019706296979014	0.0	0.0	2.492007508917026E-5	0.0
9	1.897489277514691	0.0	0.0	2.492007508917026E-5	0.0
10	1.9801242465103797	0.0	0.0	2.492007508917026E-5	0.0
11	2.058647403116355	0.0	0.0	2.492007508917026E-5	0.0
12	2.117807661378045	0.0	0.0	2.492007508917026E-5	0.0
13	2.1585270640737493	0.0	0.0	2.492007508917026E-5	0.0
14	2.2049282438897846	0.0	0.0	2.492007508917026E-5	0.0
15	2.2598520893863157	0.0	0.0	2.492007508917026E-5	0.0
16	2.299350408402651	0.0	0.0	2.492007508917026E-5	0.0
17	2.3363567199100683	0.0	0.0	2.492007508917026E-5	0.0
18	2.3662358899419837	0.0	0.0	2.492007508917026E-5	0.0
19	2.4415941970116344	0.0	0.0	2.492007508917026E-5	0.0
20	2.4656669895477727	0.0	0.0	2.492007508917026E-5	0.0
21	2.50930204102891	0.0	0.0	2.492007508917026E-5	0.0
22	2.5272195750180235	0.0	0.0	2.492007508917026E-5	0.0
23	2.546457872986863	0.0	0.0	2.492007508917026E-5	0.0
24	2.5627306820200912	0.0	0.0	2.492007508917026E-5	0.0
25	2.582193260664733	0.0	0.0	2.492007508917026E-5	0.0
26	2.644842329438907	0.0	0.0	2.492007508917026E-5	0.0
27	2.6602180157689252	0.0	0.0	2.492007508917026E-5	0.0
28	2.6749208600715355	0.0	0.0	2.492007508917026E-5	0.0
29	2.689723384674503	0.0	0.0	2.492007508917026E-5	0.0
30	2.7041770282262214	0.0	0.0	2.492007508917026E-5	0.0
31	2.7183814710270484	0.0	0.0	1.246003754458513E-4	0.0
32	2.731937991875557	0.0	0.0	1.246003754458513E-4	0.0
33	2.7449213509970147	0.0	0.0	1.246003754458513E-4	0.0
34	2.757231868091065	0.0	0.0	1.246003754458513E-4	0.0
35	2.7704893480385033	0.0	0.0	1.246003754458513E-4	0.0
36	2.784893151440044	0.0	0.0	1.246003754458513E-4	0.0
37	2.796929547708113	0.0	0.0	1.246003754458513E-4	0.0
38	2.804654770985756	0.0	0.0	1.246003754458513E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	2180	0.0	103.47429	44
CTATACG	295	0.0	79.44492	44
TCGTGCG	3100	0.0	72.48681	43
TTAAGCC	2005	0.0	70.402885	43
CGTGCGC	1500	0.0	63.961437	44
ATTAAGC	2695	0.0	50.550556	42
AATCCCG	600	0.0	49.104736	43
TACGCTA	565	0.0	48.33295	42
CTCGTGC	3745	0.0	47.3563	42
CTAATCC	2550	0.0	45.151783	41
TGTGTTA	2055	0.0	44.643795	42
ATTAGTC	390	0.0	43.191814	44
CCTTAGC	2955	0.0	42.858284	43
ACGCTAC	870	0.0	42.45803	43
ACCTTAG	2155	0.0	42.429775	42
AACGACA	315	0.0	41.88037	43
GATTAAG	2995	0.0	41.310635	41
CAATGAA	1665	0.0	40.673008	43
ATAAGAT	1490	0.0	40.432823	43
AACCTTA	2695	0.0	40.033634	41
>>END_MODULE