FastQCFastQC Report
Tue 24 May 2016
ERR841199.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841199.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4238304
Sequences flagged as poor quality0
Sequence length24-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA159120.37543319214478243No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC136850.32288858939802334No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC136130.3211897966733864No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG127300.3003559914531851No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG115250.27192480765891264No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT93970.22171604490852947No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG85030.20062270191095305No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG80900.1908782380876879No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG77190.1821247366871277No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC76520.18054391567947933No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT71100.1677557815579062No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG70860.1671895173163605No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT64540.15227789228899108No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG64010.15102739208891103No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC61730.1456478817942271No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG60860.14359517391862406No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC60400.14250983412232818No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA59490.14036274887313416No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG59070.13937178645042922No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG58830.13880552220888356No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG53510.12625333152128776No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG48500.1144325654790218No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT44280.10447575256517701No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC43510.1026589881235513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG2400.0169.244
ATGTTCT11100.0120.0405444
CTATCAT6900.0102.99129544
CGTGCGC16300.097.31595644
ACATTGG4700.091.844
CGCTACC6250.085.276844
AATCGCA2000.082.48544
ACTTCTA6800.078.3794144
GACCGTA1850.075.45405644
TCGTGCG25450.075.38183643
CTATGCG1409.822543E-1172.5142844
ACGACAC3250.070.2830744
TTCAGGA15450.065.7087344
AATCCCG14200.064.6590443
TTTGGAC23750.063.07570343
CCAGAAT9300.062.76774244
CATACAA2450.056.9755144
TATGTTC17350.055.9835743
GACCTTA4850.055.7969343
TGTGTTA16650.054.21156742
Produced by FastQC (version 0.11.5)