##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841199.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4238304
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.91700382983382	37.0	35.0	39.0	30.0	39.0
2	36.71037377215037	38.0	35.0	39.0	32.0	39.0
3	36.98306138493133	38.0	36.0	39.0	33.0	39.0
4	37.45738837988026	39.0	36.0	40.0	33.0	41.0
5	37.88249733855807	39.0	37.0	40.0	33.0	41.0
6	37.89551716913181	39.0	37.0	41.0	33.0	41.0
7	37.793662512174684	39.0	36.0	40.0	33.0	41.0
8	37.69499946204897	39.0	36.0	40.0	33.0	41.0
9	37.59315731009385	39.0	36.0	40.0	33.0	41.0
10	37.38246619402478	39.0	36.0	40.0	32.0	41.0
11	37.21544466843341	38.0	35.0	40.0	32.0	41.0
12	37.020781897664726	38.0	35.0	40.0	31.0	41.0
13	36.850608875625724	38.0	35.0	40.0	31.0	41.0
14	36.767834256344045	38.0	35.0	40.0	31.0	41.0
15	36.71540809720114	38.0	35.0	40.0	31.0	41.0
16	36.58260002114053	38.0	35.0	40.0	31.0	41.0
17	36.50147653400983	38.0	35.0	40.0	31.0	41.0
18	36.30485472490883	38.0	34.0	40.0	30.0	41.0
19	36.15520595030465	37.0	34.0	40.0	30.0	41.0
20	35.95553457231949	37.0	34.0	40.0	30.0	41.0
21	35.592068431146046	37.0	34.0	40.0	29.0	41.0
22	35.6014051847154	37.0	33.0	39.0	29.0	41.0
23	35.56679959719737	37.0	34.0	39.0	30.0	41.0
24	35.446628179573715	36.0	33.0	39.0	29.0	41.0
25	35.333262127663644	36.0	33.0	39.0	29.0	41.0
26	35.29064092590611	36.0	33.0	39.0	29.0	40.0
27	35.24928644103775	35.0	33.0	39.0	30.0	40.0
28	35.15970722498975	35.0	33.0	39.0	30.0	40.0
29	35.1329047710337	35.0	33.0	39.0	30.0	40.0
30	35.08003721749956	35.0	33.0	39.0	30.0	40.0
31	34.89151393959158	35.0	33.0	38.0	29.0	40.0
32	34.8437943774729	35.0	33.0	38.0	30.0	40.0
33	34.76305552045513	35.0	33.0	38.0	30.0	40.0
34	34.689457141209346	35.0	33.0	38.0	29.0	40.0
35	34.59521250496816	35.0	33.0	37.0	29.0	40.0
36	34.4687714029925	35.0	33.0	37.0	29.0	40.0
37	34.39593586159135	35.0	33.0	37.0	29.0	40.0
38	34.324851934327974	35.0	33.0	37.0	29.0	39.0
39	34.19007317713968	35.0	33.0	36.0	29.0	39.0
40	34.045592603233125	35.0	33.0	36.0	29.0	39.0
41	33.892575666550215	35.0	33.0	36.0	29.0	39.0
42	33.789761660705196	35.0	33.0	36.0	29.0	39.0
43	33.68323643424947	35.0	33.0	35.0	29.0	39.0
44	33.63948114478423	35.0	33.0	35.0	29.0	38.0
45	33.60759864247672	35.0	33.0	35.0	29.0	37.0
46	33.52715317112978	35.0	33.0	35.0	29.0	37.0
47	33.47196539625029	35.0	33.0	35.0	29.0	37.0
48	33.50247928941719	35.0	33.0	35.0	29.0	37.0
49	33.44683941901537	34.0	33.0	35.0	29.0	37.0
50	33.80321661793003	35.0	33.0	36.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	17.0
22	58.0
23	227.0
24	702.0
25	2193.0
26	6220.0
27	15097.0
28	33928.0
29	67848.0
30	119898.0
31	193596.0
32	288967.0
33	399187.0
34	521401.0
35	592200.0
36	659614.0
37	595245.0
38	410949.0
39	264757.0
40	66195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.601378287163925	17.943380182261585	37.4552415305745
2	28.631523364062605	22.142725014534115	28.185307141724614	21.040444479678662
3	29.939593762033116	15.82147009747295	26.41188550892055	27.827050631573385
4	20.25451690110006	17.039150565886732	32.420562564648506	30.285769968364708
5	23.328977817542114	19.065881069408896	28.68246355145832	28.922677561590675
6	27.591060008909224	17.790229299266876	25.36743470973295	29.251275982090952
7	31.8337004613166	17.190036391915257	21.876085339796294	29.100177806971843
8	29.421226037584848	16.395024991128526	25.544392285215974	28.639356686070656
9	28.80451708985481	16.768688607518477	23.610977409831857	30.815816892794857
10	28.863856863500114	18.43126401503998	26.091049627398128	26.613829494061775
11	33.84691140607186	17.030774573980537	23.402851706720423	25.719462313227176
12	31.084108171570513	17.2291794076121	23.070525379963307	28.61618704085408
13	29.441823899371066	17.70526606869163	23.754336640316502	29.098573391620796
14	28.89073082063014	19.159078725829954	21.096528233935082	30.85366221960482
15	27.354219989882743	19.692877150860344	21.97152445884014	30.98137840041677
16	29.364717585147265	19.395069348494115	23.469741670253008	27.770471396105613
17	29.10211254313046	18.398585849434113	18.783197241160615	33.71610436627481
18	29.602289028819072	18.96253784532681	21.113586944211647	30.321586181642466
19	29.78696195459316	17.64571394595574	23.764765340098304	28.8025587593528
20	31.01535425490951	17.554262270946115	23.037139384055507	28.393244090088864
21	31.377574614751563	20.560936638806464	21.628745837957826	26.432742908484148
22	31.617293143672565	18.91209313914245	22.25359483416008	27.217018883024906
23	30.844082916185343	21.642595717532295	22.284621395728102	25.22869997055426
24	34.522747778356624	21.19076404146564	19.105826292781263	25.180661887396464
25	30.49781843032119	21.50303699349591	21.992587919886454	26.006556656296443
26	31.61284136580861	21.62516607442211	20.888944062536	25.87304849723328
27	31.274353600245945	20.96311550407835	21.789228663379262	25.97330223229644
28	30.546023471399764	21.06524727695724	21.592613033975084	26.796116217667908
29	32.837822697701746	20.175350364618218	22.885749639614296	24.101077298065743
30	30.591900421083253	22.43210933745767	21.9960295639981	24.97996067746098
31	30.134991342208277	20.809632623200237	24.05238072101713	25.002995313574356
32	28.52414763788881	19.787874234771518	24.70141481387306	26.98656331346661
33	28.03606934160452	19.08866090765982	25.59332188381952	27.28194786691614
34	27.944094038200397	17.86025868893887	28.798307564056184	25.397339708804545
35	30.147142349876756	18.370590912810037	23.746983686420425	27.735283050892782
36	28.006509052319988	21.24341098964627	21.77173620673735	28.978343751296393
37	27.00720301966408	21.51159381637907	25.937112669148828	25.544090494808025
38	30.01670414496511	20.28726323963213	21.97932923492941	27.71670338047335
39	27.451031257865242	21.631301579451673	24.44195393670713	26.475713225975955
40	28.7489394144338	19.67938176944239	22.910157363510045	28.661521452613766
41	27.247120868866542	20.38536389533603	24.685927122975237	27.68158811282219
42	27.382167203079415	20.740321274201296	25.591145964001804	26.286365558717485
43	24.93212215539248	22.44415514520317	25.42123828156251	27.202484417841845
44	24.379502291592832	22.767493406746144	25.16520390912007	27.687800392540957
45	27.135363385585876	19.45419797234493	27.323392226063703	26.087046416005492
46	27.00417430055883	20.73344179305921	23.58986784802201	28.67251605835995
47	28.362024381724023	20.888474296153454	23.8979243787716	26.851576943350924
48	25.57565540241023	21.644877502373014	24.491807293083085	28.287659802133675
49	28.02474280414578	21.160982608302003	22.379028279646153	28.435246307906066
50	25.331585885062346	23.980394236450962	23.233132831320738	27.454887047165954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1481.0
1	1499.0
2	1561.0
3	1575.5
4	1474.0
5	1174.0
6	853.0
7	861.5
8	1053.5
9	1220.5
10	1315.5
11	1312.0
12	1270.0
13	1277.0
14	1389.0
15	1544.5
16	1644.5
17	1805.5
18	2076.5
19	2470.5
20	2901.0
21	3691.5
22	4765.0
23	6049.5
24	7334.0
25	8530.0
26	10330.5
27	12763.5
28	15484.0
29	18408.5
30	21715.0
31	26657.5
32	33005.5
33	37607.0
34	41949.5
35	48219.5
36	54106.0
37	58806.0
38	64434.5
39	74023.5
40	85776.5
41	103714.5
42	127536.0
43	144604.5
44	154106.0
45	165259.5
46	184085.0
47	202819.0
48	227124.0
49	257911.5
50	292109.0
51	319896.0
52	334800.5
53	350209.0
54	354919.0
55	354450.0
56	359463.0
57	369743.5
58	375279.0
59	362263.5
60	349110.5
61	344244.5
62	334270.0
63	317355.5
64	288920.0
65	257645.0
66	238107.5
67	226509.5
68	211685.0
69	191799.0
70	171233.0
71	152268.0
72	137625.5
73	126047.5
74	110978.5
75	94731.0
76	82376.0
77	66555.0
78	52123.5
79	42815.0
80	33421.0
81	23698.0
82	16656.5
83	12434.5
84	9550.5
85	7463.0
86	5660.5
87	4003.0
88	2745.0
89	2019.5
90	1408.0
91	840.0
92	566.5
93	441.0
94	290.5
95	136.5
96	88.0
97	87.0
98	48.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	53244.0
25	44597.0
26	41993.0
27	41703.0
28	40237.0
29	35607.0
30	49782.0
31	37696.0
32	39424.0
33	39401.0
34	38073.0
35	40212.0
36	35526.0
37	38245.0
38	39028.0
39	45324.0
40	46070.0
41	44467.0
42	50250.0
43	48734.0
44	51437.0
45	72755.0
46	250028.0
47	539770.0
48	751695.0
49	1062996.0
50	660010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.133844911193023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.17122562638301	12.08247815770803
2	12.928546006800056	5.4645977246987245
3	6.811839602713683	4.31881085171021
4	4.42852643047246	3.743671630668968
5	3.2110432763520014	3.393084530277615
6	2.526503990804507	3.2036846105503622
7	1.9912381515647046	2.945776279349339
8	1.5723397558590992	2.658366763842345
9	1.2983465488098278	2.4695149143176645
>10	7.3690616538995375	27.021460930002593
>50	0.3766253334738644	5.479206486880634
>100	0.26325497860800834	10.96469148957605
>500	0.03079586307234791	4.492775948381043
>1k	0.018086459264381557	7.183668727353694
>5k	0.0019552928934466547	2.8358068723563097
>10k+	6.110290292020797E-4	1.7424040823263764
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	15912	0.37543319214478243	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	13685	0.32288858939802334	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	13613	0.3211897966733864	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	12730	0.3003559914531851	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	11525	0.27192480765891264	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	9397	0.22171604490852947	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	8503	0.20062270191095305	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	8090	0.1908782380876879	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	7719	0.1821247366871277	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	7652	0.18054391567947933	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	7110	0.1677557815579062	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	7086	0.1671895173163605	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	6454	0.15227789228899108	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	6401	0.15102739208891103	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	6173	0.1456478817942271	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	6086	0.14359517391862406	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	6040	0.14250983412232818	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	5949	0.14036274887313416	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	5907	0.13937178645042922	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	5883	0.13880552220888356	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	5351	0.12625333152128776	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	4850	0.1144325654790218	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	4428	0.10447575256517701	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	4351	0.1026589881235513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05606015991302181	0.0	0.0	0.0	0.0
2	0.2245237717728601	0.0	0.0	0.0	0.0
3	0.3192786548581697	0.0	0.0	0.0	0.0
4	0.3907223266665157	0.0	0.0	0.0	0.0
5	0.5085524775948115	0.0	0.0	0.0	0.0
6	0.6127215036958179	0.0	0.0	0.0	0.0
7	0.6989116401277492	0.0	0.0	0.0	0.0
8	0.7667453773962415	0.0	0.0	0.0	0.0
9	0.828303019320936	0.0	0.0	0.0	0.0
10	0.8839148867094008	0.0	0.0	0.0	0.0
11	0.9412963298526958	0.0	0.0	0.0	0.0
12	0.9860312049348041	0.0	0.0	0.0	0.0
13	1.0187565592274646	0.0	0.0	0.0	0.0
14	1.0580411409846957	0.0	0.0	0.0	0.0
15	1.104215271014066	0.0	0.0	0.0	0.0
16	1.1311128224874856	0.0	0.0	0.0	0.0
17	1.1656077525349762	0.0	0.0	0.0	0.0
18	1.1991589088465575	0.0	0.0	0.0	0.0
19	1.2924981313280028	0.0	0.0	0.0	0.0
20	1.3157385595747733	0.0	0.0	0.0	0.0
21	1.3691797473706464	0.0	0.0	0.0	0.0
22	1.3843745045187887	0.0	0.0	0.0	0.0
23	1.400536629746238	0.0	0.0	0.0	0.0
24	1.4144808866943004	0.0	0.0	0.0	0.0
25	1.43616408827682	0.0	0.0	0.0	0.0
26	1.5187442901688977	0.0	0.0	0.0	0.0
27	1.5319571224716302	4.7188686795472906E-5	0.0	0.0	0.0
28	1.5464676436612381	4.7188686795472906E-5	0.0	0.0	0.0
29	1.5593265608130045	4.7188686795472906E-5	0.0	0.0	0.0
30	1.5716899967534184	4.7188686795472906E-5	0.0	0.0	0.0
31	1.5826141777465703	4.7188686795472906E-5	0.0	0.0	0.0
32	1.5941754060114612	4.7188686795472906E-5	0.0	0.0	0.0
33	1.6062557098311023	4.7188686795472906E-5	0.0	0.0	0.0
34	1.6158822019373786	4.7188686795472906E-5	0.0	0.0	0.0
35	1.627490618889065	4.7188686795472906E-5	0.0	0.0	0.0
36	1.6378957243274668	4.7188686795472906E-5	0.0	0.0	0.0
37	1.6476637824941298	4.7188686795472906E-5	0.0	0.0	0.0
38	1.6535151796567684	4.7188686795472906E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	240	0.0	169.2	44
ATGTTCT	1110	0.0	120.04054	44
CTATCAT	690	0.0	102.991295	44
CGTGCGC	1630	0.0	97.315956	44
ACATTGG	470	0.0	91.8	44
CGCTACC	625	0.0	85.2768	44
AATCGCA	200	0.0	82.485	44
ACTTCTA	680	0.0	78.37941	44
GACCGTA	185	0.0	75.454056	44
TCGTGCG	2545	0.0	75.381836	43
CTATGCG	140	9.822543E-11	72.51428	44
ACGACAC	325	0.0	70.28307	44
TTCAGGA	1545	0.0	65.70873	44
AATCCCG	1420	0.0	64.65904	43
TTTGGAC	2375	0.0	63.075703	43
CCAGAAT	930	0.0	62.767742	44
CATACAA	245	0.0	56.97551	44
TATGTTC	1735	0.0	55.98357	43
GACCTTA	485	0.0	55.79693	43
TGTGTTA	1665	0.0	54.211567	42
>>END_MODULE