FastQCFastQC Report
Tue 24 May 2016
ERR841197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801962
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG220250.786056341948963No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA145950.5208850084333763No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG121930.43515936333183675No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC112160.40029093899203483No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC88690.31652820416550975Illumina Single End Adapter 2 (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTGCAGT84800.30264507512949856No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT80500.2872986857066584Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTG80410.2869774822071106No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCG77930.2781265413306819Illumina Single End Adapter 2 (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT77630.2770558629988558No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC73720.26310135540738955No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGG70720.25239457208912897No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG61180.2183470011370604Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTG60320.21527772325249236No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT59800.21342188081066052No Hit
AAGCAGTGGTATCAACGCAGAGCG57100.20378577582422602No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG55980.1997885767187421No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT53150.18968851112184962No Hit
AAGCAGTGGTATCAACGCAGAGTGCA49150.17541280003083554No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA41550.14828894895790878No Hit
AAGCAGTGGTATCAACGCAGAGTGC41130.14678999929335232No Hit
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTG38640.13790336913919604Illumina Single End Adapter 2 (100% over 30bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC36110.12887398187412963No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC35960.1283386427082166No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG35840.1279103713754862Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG35660.12726796437639054Illumina Single End Adapter 2 (100% over 34bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG33290.1188096055549647No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG32940.11756048083450096No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA31760.11334914606265181No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT31620.11284949617446631No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT29310.1046052730194057Illumina Single End Adapter 2 (95% over 24bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC29150.10403424457576511No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA28380.10128617019074491No Hit
ACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGTTGCCGTCCCTTCGCCT28380.10128617019074491No Hit
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTC28220.10071514174710436Illumina Single End Adapter 2 (100% over 28bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG28210.10067945246937682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG25400.071.81483543
CGTGCGC14250.070.3992544
CCAGAAT12350.068.9053644
AATCCCG7050.068.5653443
AATCGCA2400.066.30273444
CTATACG1001.6370905E-1162.2669244
TAATCGC1950.053.7874743
CTATCAT4900.053.6540344
ATAAGAT9600.049.40262243
ATGTTCT5850.048.48895344
CTAATCG2250.048.0854442
TAAGCCA6250.045.38566244
TGTGTTA10050.044.32810642
CAATGAA10550.044.08954243
CTCGTGC35600.043.62013642
AGGTCGA751.8774335E-742.56225643
TAATCCA19150.041.58877640
CATAAGA9450.041.41813342
TCCAGAA23400.041.31501443
TACGCTA4550.041.2627242
Produced by FastQC (version 0.11.5)