##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841197.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2801962
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.350884487369925	37.0	35.0	39.0	30.0	39.0
2	36.36373227045906	37.0	35.0	39.0	32.0	39.0
3	36.5900608216671	38.0	35.0	39.0	32.0	39.0
4	36.80018751146518	38.0	35.0	39.0	33.0	40.0
5	37.57424333377826	39.0	36.0	40.0	33.0	41.0
6	37.641590428421225	39.0	36.0	40.0	33.0	41.0
7	37.06424855155066	38.0	35.0	40.0	31.0	41.0
8	37.31938370327649	38.0	35.0	40.0	33.0	41.0
9	37.34097571630165	39.0	35.0	40.0	33.0	41.0
10	36.46320078573514	38.0	35.0	40.0	30.0	41.0
11	36.76859250767855	38.0	35.0	40.0	31.0	41.0
12	36.81081256633745	38.0	35.0	40.0	31.0	41.0
13	36.784756538454126	38.0	35.0	40.0	31.0	41.0
14	36.752943830073356	38.0	35.0	40.0	31.0	41.0
15	36.72795598227242	38.0	35.0	40.0	31.0	41.0
16	36.54217187813396	38.0	35.0	40.0	31.0	41.0
17	36.37277950236299	38.0	34.0	40.0	31.0	41.0
18	36.35736066370636	38.0	34.0	40.0	31.0	41.0
19	36.26231512061905	38.0	34.0	40.0	30.0	41.0
20	36.05394612774906	38.0	34.0	40.0	30.0	41.0
21	35.83469476031438	37.0	34.0	40.0	30.0	41.0
22	35.69781995615929	37.0	34.0	40.0	30.0	41.0
23	35.223839224086554	37.0	33.0	39.0	28.0	41.0
24	35.49112336284361	37.0	33.0	39.0	30.0	41.0
25	35.5427213982381	37.0	33.0	39.0	30.0	41.0
26	35.6341471685442	37.0	34.0	39.0	30.0	41.0
27	35.645389014176764	37.0	34.0	39.0	30.0	41.0
28	35.34399220234934	36.0	33.0	39.0	29.0	41.0
29	35.38678892550124	36.0	33.0	39.0	29.0	41.0
30	35.52288068446922	36.0	34.0	39.0	30.0	41.0
31	35.4637411787179	36.0	34.0	39.0	30.0	41.0
32	35.46077935212419	36.0	34.0	39.0	30.0	41.0
33	35.40288980699084	36.0	34.0	39.0	30.0	41.0
34	35.34697389585794	35.0	34.0	39.0	30.0	40.0
35	35.2323961620605	35.0	34.0	39.0	30.0	40.0
36	35.1047129983554	35.0	33.0	39.0	30.0	40.0
37	35.01924972648337	35.0	33.0	38.0	30.0	40.0
38	34.94942616772542	35.0	33.0	38.0	30.0	40.0
39	34.792485338793696	35.0	33.0	38.0	29.0	40.0
40	34.62677960451793	35.0	33.0	38.0	29.0	40.0
41	34.47936448515112	35.0	33.0	37.0	29.0	40.0
42	34.315835543119256	35.0	33.0	37.0	29.0	40.0
43	34.21146674328132	35.0	33.0	37.0	29.0	40.0
44	34.11156457279598	35.0	33.0	36.0	29.0	39.0
45	34.0223350401208	35.0	33.0	36.0	29.0	39.0
46	33.9568498954526	35.0	33.0	36.0	29.0	39.0
47	33.937855824722995	35.0	33.0	36.0	29.0	39.0
48	33.911912451085314	35.0	33.0	36.0	29.0	39.0
49	33.860742372798306	35.0	33.0	36.0	29.0	39.0
50	34.02817065833311	35.0	33.0	36.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	14.0
22	68.0
23	242.0
24	679.0
25	1903.0
26	4423.0
27	10487.0
28	22626.0
29	44405.0
30	79001.0
31	127153.0
32	188438.0
33	264392.0
34	349222.0
35	339176.0
36	391449.0
37	387610.0
38	312915.0
39	210381.0
40	67373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	57.8203416034907	18.75649990970613	23.423158486803175
2	24.482344871200965	32.47299570800746	17.991714377282776	25.0529450435088
3	37.52988084777738	16.93338453555045	21.023804034458713	24.512930582213464
4	19.15971736947182	17.567939893546022	25.356982000469667	37.91536073651249
5	20.040849947286937	30.837570245420885	23.013516957046527	26.108062850245652
6	36.09549308663001	18.589259954274898	22.14323391966058	23.17201303943451
7	28.79243187452221	18.734229800404144	29.770603598478495	22.70273472659515
8	37.71696404162512	17.269506153188374	22.170857420621694	22.842672384564818
9	36.84286225152233	18.221838840069925	21.585053616001932	23.350245292405823
10	26.0472126317202	18.107954354841358	33.20077145942736	22.64406155401108
11	29.02476907252846	27.87050645226452	21.996800813144503	21.10792366206251
12	28.51034382336377	18.212202735083487	30.452732763684875	22.824720677867866
13	25.64096158334767	18.070480613227446	22.177245801334923	34.11131200208997
14	23.794398353724997	29.359820011834564	19.832531633191312	27.013250001249123
15	22.53549477116392	30.234278694714632	21.607823375192098	25.62240315892935
16	24.55179620565875	21.774064030846958	20.972554231641972	32.701585531852324
17	35.54130998207685	20.547530623184755	17.274110070015226	26.63704932472318
18	23.969739775200377	19.981106096371043	21.807968844688112	34.241185283740464
19	24.545479203500975	28.514733604524256	22.193627179811863	24.746160012162907
20	35.34173554102446	17.519366786558844	21.80511370246991	25.333783969946772
21	28.014940959227857	28.06183667016184	20.706811869682742	23.216410500927566
22	34.452537186442925	19.785778679368242	21.548936066941664	24.212748067247166
23	26.129654863270808	21.062990861403545	28.109517545205826	24.697836730119825
24	37.44940152650179	19.23691327719648	19.857871020377864	23.45581417592387
25	27.479771466447854	21.773736956747676	21.608572697348578	29.137918879455892
26	27.10196941510977	27.523710495868286	20.56295541641679	24.811364672605148
27	32.594825741560435	21.368061413837232	21.345169769735453	24.691943074866877
28	27.806941187391665	22.717581693478063	26.250949216354368	23.224527902775897
29	30.195009370741676	21.446522253859648	23.839384477139046	24.51908389825963
30	27.7031349212906	22.382628304539708	23.087185007014487	26.8270517671552
31	29.04650028033884	20.549336238360343	25.690866388872124	24.713297092428693
32	27.871698758454812	20.84017936987155	26.229419851837925	25.05870201983571
33	25.267298331015297	20.51341453013882	28.243498569816566	25.975788569029312
34	25.76290683146364	21.097368354486544	28.169674642644328	24.970050171405493
35	28.995309110492595	19.968198923242888	25.30526013800879	25.73123182825573
36	26.964521127197383	22.553549268045646	23.819137578188496	26.66279202656848
37	25.272721647304923	22.554271457411765	27.59118868221278	24.581818213070527
38	27.808804876161595	22.27861097901411	22.95546442552873	26.957119719295562
39	27.402534697013486	22.726015182806915	25.28384060167335	24.587609518506248
40	25.83618679250932	22.39286625524425	25.014042012987915	26.75690493925852
41	25.98928692663326	24.910637522587052	23.213849495776724	25.886226055002965
42	25.20873440621721	23.204570999167043	25.868700268305382	25.717994326310368
43	23.573558959750166	24.73742479301409	26.067075881263506	25.621940365972236
44	23.411098155062973	23.05981065570496	25.66733687268051	27.86175431655155
45	26.424022146293304	22.11677136468789	26.08969829547104	25.369508193547762
46	24.423826815877355	23.013191226031633	25.024696139784357	27.53828581830665
47	27.961915022725464	21.65270623079671	24.433267313964993	25.95211143251283
48	24.564540363498807	24.15519009617881	24.00869568282021	27.27157385750217
49	26.504383323012508	23.132134991311077	23.5738043121051	26.789677373571312
50	23.55743881647058	22.99040794151115	25.827614916760638	27.624538325257635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1837.0
1	1848.0
2	1623.5
3	1374.0
4	1301.5
5	1103.0
6	880.0
7	821.0
8	854.0
9	871.5
10	917.5
11	974.0
12	976.0
13	1011.5
14	1131.5
15	1331.0
16	1529.5
17	1727.5
18	2043.0
19	2572.0
20	3117.0
21	3936.5
22	4888.0
23	5970.5
24	7255.5
25	8691.5
26	10418.0
27	12980.5
28	15863.5
29	18018.0
30	20234.0
31	24292.5
32	29207.0
33	33376.0
34	38986.5
35	47090.0
36	53312.5
37	57076.0
38	60732.0
39	67777.5
40	77321.5
41	91450.0
42	110895.5
43	129362.5
44	140046.0
45	142324.0
46	146283.0
47	156439.5
48	191916.5
49	234893.5
50	289031.0
51	355009.0
52	384041.5
53	376582.5
54	342227.0
55	273289.0
56	212272.5
57	192567.0
58	187080.5
59	180124.0
60	171745.0
61	166731.5
62	165652.5
63	158888.0
64	141555.5
65	125154.5
66	112281.0
67	100480.5
68	90790.0
69	80711.0
70	70481.5
71	63255.5
72	57778.5
73	50988.5
74	42227.5
75	34434.5
76	28895.0
77	23175.0
78	18233.0
79	14395.5
80	10762.0
81	8084.5
82	6177.5
83	4713.0
84	3541.5
85	2644.5
86	1907.5
87	1287.5
88	823.0
89	569.0
90	381.0
91	231.5
92	163.5
93	139.0
94	98.0
95	46.5
96	28.0
97	24.0
98	15.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	50156.0
25	38767.0
26	35206.0
27	49349.0
28	55582.0
29	31020.0
30	39553.0
31	34356.0
32	29125.0
33	27315.0
34	27337.0
35	30429.0
36	26669.0
37	26600.0
38	30562.0
39	32009.0
40	31464.0
41	34684.0
42	34677.0
43	34266.0
44	36576.0
45	44689.0
46	98398.0
47	220719.0
48	518763.0
49	404192.0
50	779499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.186502321776562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.92154413437785	17.17165245189855
2	14.709161553694953	8.291996325692187
3	7.550249460245293	6.384453718235887
4	4.560626658205752	5.141924555610901
5	2.9263341442368485	4.124156207541298
6	1.993896948282344	3.3720588577286095
7	1.3973906469473119	2.7571288300226486
8	1.0439591565729291	2.3540445752466215
9	0.7895716398841911	2.0029736574724213
>10	3.69512289264911	17.52053998499525
>50	0.2116655684865943	4.146046010718899
>100	0.16445967388193486	9.328435036167157
>500	0.019414649693554453	3.8272963541612732
>1k	0.014192778396667381	7.605983493395713
>5k	0.001874517901446635	3.705811056917897
>10k+	5.355765432704672E-4	2.265498884194641
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTGCAG	22025	0.786056341948963	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA	14595	0.5208850084333763	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	12193	0.43515936333183675	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	11216	0.40029093899203483	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC	8869	0.31652820416550975	Illumina Single End Adapter 2 (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTGCAGT	8480	0.30264507512949856	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	8050	0.2872986857066584	Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTG	8041	0.2869774822071106	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCG	7793	0.2781265413306819	Illumina Single End Adapter 2 (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT	7763	0.2770558629988558	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	7372	0.26310135540738955	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGG	7072	0.25239457208912897	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG	6118	0.2183470011370604	Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTG	6032	0.21527772325249236	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	5980	0.21342188081066052	No Hit
AAGCAGTGGTATCAACGCAGAGCG	5710	0.20378577582422602	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	5598	0.1997885767187421	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	5315	0.18968851112184962	No Hit
AAGCAGTGGTATCAACGCAGAGTGCA	4915	0.17541280003083554	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	4155	0.14828894895790878	No Hit
AAGCAGTGGTATCAACGCAGAGTGC	4113	0.14678999929335232	No Hit
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTG	3864	0.13790336913919604	Illumina Single End Adapter 2 (100% over 30bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	3611	0.12887398187412963	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	3596	0.1283386427082166	No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	3584	0.1279103713754862	Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	3566	0.12726796437639054	Illumina Single End Adapter 2 (100% over 34bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	3329	0.1188096055549647	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	3294	0.11756048083450096	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	3176	0.11334914606265181	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	3162	0.11284949617446631	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT	2931	0.1046052730194057	Illumina Single End Adapter 2 (95% over 24bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	2915	0.10403424457576511	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	2838	0.10128617019074491	No Hit
ACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGTTGCCGTCCCTTCGCCT	2838	0.10128617019074491	No Hit
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTC	2822	0.10071514174710436	Illumina Single End Adapter 2 (100% over 28bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	2821	0.10067945246937682	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.18497752646181498	0.0	0.0	0.0	0.0
2	1.2719301689316271	0.0	0.0	0.0	0.0
3	1.5319622464544487	0.0	0.0	0.0	0.0
4	1.6507004734539583	0.0	0.0	0.0	0.0
5	2.500569243979754	3.568927772753521E-5	0.0	0.0	0.0
6	2.695611146760734	3.568927772753521E-5	0.0	0.0	0.0
7	2.833907097954933	3.568927772753521E-5	0.0	0.0	0.0
8	2.941189066803904	3.568927772753521E-5	0.0	0.0	0.0
9	3.0132457185357975	3.568927772753521E-5	0.0	0.0	0.0
10	3.09226177942456	3.568927772753521E-5	0.0	0.0	0.0
11	3.265212019292196	3.568927772753521E-5	0.0	0.0	0.0
12	3.321494010268519	3.568927772753521E-5	0.0	0.0	0.0
13	3.367426110703857	3.568927772753521E-5	0.0	0.0	0.0
14	3.4510460884194716	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
15	3.653047400357321	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
16	3.700585518290398	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
17	3.827639347000423	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
18	3.890416786523158	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
19	4.62768588581858	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
20	4.687144222512654	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
21	5.04557163873029	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
22	5.077085270963703	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
23	5.115237108854439	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
24	5.142039756427818	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
25	5.204388924617821	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
26	6.077562793499697	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
27	6.1079343688458305	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
28	6.133916163031476	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
29	6.159933646494849	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
30	6.176600539193608	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
31	6.190376600396436	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
32	6.203189051100622	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
33	6.215751676860714	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
34	6.2297775630076355	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
35	6.243946206265467	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
36	6.257615199635113	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
37	6.27053471817248	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
38	6.2797425518261845	3.568927772753521E-5	0.0	1.0706783318260561E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTGCG	2540	0.0	71.814835	43
CGTGCGC	1425	0.0	70.39925	44
CCAGAAT	1235	0.0	68.90536	44
AATCCCG	705	0.0	68.56534	43
AATCGCA	240	0.0	66.302734	44
CTATACG	100	1.6370905E-11	62.26692	44
TAATCGC	195	0.0	53.78747	43
CTATCAT	490	0.0	53.65403	44
ATAAGAT	960	0.0	49.402622	43
ATGTTCT	585	0.0	48.488953	44
CTAATCG	225	0.0	48.08544	42
TAAGCCA	625	0.0	45.385662	44
TGTGTTA	1005	0.0	44.328106	42
CAATGAA	1055	0.0	44.089542	43
CTCGTGC	3560	0.0	43.620136	42
AGGTCGA	75	1.8774335E-7	42.562256	43
TAATCCA	1915	0.0	41.588776	40
CATAAGA	945	0.0	41.418133	42
TCCAGAA	2340	0.0	41.315014	43
TACGCTA	455	0.0	41.26272	42
>>END_MODULE