FastQCFastQC Report
Tue 24 May 2016
ERR841196.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841196.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686064
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT66130.3922152421260403No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG65220.3868180567285702No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA62540.3709230491843726No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA59010.3499867146205601No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA56040.3323717249167291No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC46130.2735957828409835No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC38680.22941003425729986No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG36420.21600603535808843No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT32520.19287524079750235No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG32010.18985044458573339No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG31750.18830839161502766No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC30310.17976779054650358No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG28580.16950720731834618No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC25400.15064671329202214No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC25160.14922327978060143No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG25010.1483336338359635No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG24630.14607986410954743No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT23140.13724271439281072No Hit
ATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA22780.1351075641256797Illumina PCR Primer Index 11 (96% over 26bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG22690.13457377655889693No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT21910.1299476176467797No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG21640.12834625494643145No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT21190.12567731711251767No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC21130.1253214587346625No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC21000.12455043224930964No Hit
ATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAA19100.11328158361722923Illumina PCR Primer Index 11 (96% over 26bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG19020.11280710578008901No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA18670.11073126524260052No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG17690.10491891173763272No Hit
ATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA17220.1021313544444339Illumina PCR Primer Index 11 (96% over 26bp)
AGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGG17170.10183480579622126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT13050.0120.34778644
GACCGTA505.260554E-792.9312844
AATGAAG9950.084.9924244
CGTGCGC6350.083.41862544
CAATGAA13450.076.8021943
TCGTGCG12250.072.7212243
CCAGAAT7550.072.6217944
CTATGCG400.002262061769.69845644
CATGACC5600.068.0389644
AGGTCCC8900.062.29242743
ATAAGAT8550.057.637743
CCTATCA14250.056.19675443
CTATACG500.005475376355.75876644
TACATAT500.005475376355.75876644
TGCACCG1202.789966E-854.2099144
TCCAGAA18100.052.8825343
CCCTATC13150.052.8801542
ATGTTCT5450.052.85999344
GCAATGA16250.052.21439442
GGTCCCA5350.052.11099244
Produced by FastQC (version 0.11.5)