##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841196.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1686064
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1765496446161	38.0	35.0	39.0	31.0	39.0
2	36.85018421602027	39.0	35.0	39.0	32.0	39.0
3	37.1298776321658	39.0	37.0	39.0	33.0	39.0
4	37.443384118277834	39.0	37.0	40.0	33.0	41.0
5	38.017191518234185	39.0	37.0	41.0	33.0	41.0
6	38.059562982188105	40.0	37.0	41.0	33.0	41.0
7	37.994538166997216	40.0	37.0	41.0	33.0	41.0
8	37.920561141214094	39.0	37.0	41.0	33.0	41.0
9	37.80177205610226	39.0	36.0	41.0	33.0	41.0
10	37.62796667267672	39.0	36.0	40.0	33.0	41.0
11	37.49274642006472	39.0	36.0	40.0	32.0	41.0
12	37.35020556752294	39.0	36.0	40.0	32.0	41.0
13	37.21281635809791	39.0	35.0	40.0	32.0	41.0
14	37.150669844086586	38.0	35.0	40.0	32.0	41.0
15	37.078911595289384	38.0	35.0	40.0	31.0	41.0
16	37.00891840404635	38.0	35.0	40.0	31.0	41.0
17	36.94417471697397	38.0	35.0	40.0	31.0	41.0
18	36.8258399443912	38.0	35.0	40.0	31.0	41.0
19	36.74778181611137	38.0	35.0	40.0	31.0	41.0
20	36.594781692747134	38.0	35.0	40.0	31.0	41.0
21	36.35982145398989	38.0	35.0	40.0	30.0	41.0
22	36.33252415092191	38.0	34.0	40.0	30.0	41.0
23	36.27164034105467	38.0	34.0	40.0	30.0	41.0
24	36.22681464048814	38.0	34.0	40.0	30.0	41.0
25	36.204116647326885	38.0	34.0	40.0	30.0	41.0
26	36.17193114605046	37.0	34.0	40.0	30.0	41.0
27	36.15338583733107	37.0	34.0	40.0	31.0	41.0
28	36.101203651186	37.0	34.0	40.0	31.0	41.0
29	36.083262916128454	37.0	34.0	40.0	31.0	41.0
30	36.09670141633924	37.0	34.0	40.0	31.0	41.0
31	35.99229128862634	37.0	34.0	40.0	31.0	41.0
32	35.91901492161607	36.0	34.0	40.0	31.0	41.0
33	35.795944293412845	36.0	34.0	39.0	31.0	41.0
34	35.74631389703707	36.0	34.0	39.0	31.0	41.0
35	35.63848648705156	36.0	34.0	39.0	30.0	41.0
36	35.523804183995544	35.0	34.0	39.0	30.0	41.0
37	35.42869498398294	35.0	34.0	39.0	30.0	41.0
38	35.371585986756706	35.0	34.0	39.0	30.0	41.0
39	35.218574220789876	35.0	34.0	39.0	30.0	40.0
40	35.038473055742905	35.0	33.0	39.0	30.0	40.0
41	34.872991106691174	35.0	33.0	38.0	30.0	40.0
42	34.78740761832537	35.0	33.0	38.0	30.0	40.0
43	34.6678631454983	35.0	33.0	38.0	29.0	40.0
44	34.56724280968442	35.0	33.0	37.0	29.0	40.0
45	34.513336173400056	35.0	33.0	37.0	29.0	40.0
46	34.40620488124744	35.0	33.0	37.0	29.0	40.0
47	34.37728269998242	35.0	33.0	37.0	29.0	40.0
48	34.40296922788066	35.0	33.0	37.0	29.0	40.0
49	34.51542934598059	35.0	33.0	37.0	29.0	40.0
50	34.9211263257701	35.0	33.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	6.0
22	24.0
23	74.0
24	250.0
25	661.0
26	1722.0
27	4301.0
28	9346.0
29	19133.0
30	35066.0
31	59236.0
32	90203.0
33	129811.0
34	178013.0
35	198191.0
36	232772.0
37	251470.0
38	223986.0
39	176068.0
40	75731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	59.20854724375825	13.834290987767961	26.957161768473792
2	28.783604892815458	17.617540022205564	34.699572495468736	18.899282589510243
3	41.331111986259124	13.85493077368356	19.823268867611194	24.990688372446122
4	22.94029170897427	20.08873921749115	26.571589216067714	30.399379857466858
5	25.185046356484687	21.426648098767306	25.42204803613623	27.966257508611776
6	28.69641959024094	21.302097666517998	22.162207365793943	27.83927537744712
7	32.79851773123678	23.662387667372055	20.466720124502984	23.072374476888186
8	30.753577562892037	19.124422323233283	23.474197895216314	26.647802218658367
9	29.890383757674677	19.866683589709524	24.430863834350298	25.812068818265498
10	33.00699143093026	18.50908387819205	24.86673103749324	23.61719365338445
11	34.06982178612437	17.887755150456922	23.824718397403657	24.21770466601505
12	32.75367957562702	18.383584490268458	24.928769014699323	23.933966919405194
13	28.978733903339375	17.50959631425616	25.854831133337765	27.6568386490667
14	26.77258988982625	21.508614145133283	22.50999962041773	29.208796344622744
15	25.187418745670392	21.955631577449015	25.577024359692157	27.279925317188436
16	27.26379307072567	24.656596665369758	24.469059300240087	23.610550963664487
17	29.646027671547465	23.574372028582545	19.32405887321003	27.455541426659963
18	28.064889589007297	22.43853139619848	22.142753774471196	27.353825240323026
19	29.873183936078345	19.49753983241443	24.51004232342307	26.119233908084155
20	30.10757598762562	19.24405004792226	22.648962316970174	27.99941164748195
21	30.660639216542197	21.703624536197914	21.99157327361239	25.6441629736475
22	31.182742766585374	20.404029740270833	22.48396264910466	25.929264844039135
23	29.688374818512226	20.92524364436937	23.02166465804382	26.364716879074578
24	33.042577268715775	20.748204101386424	21.849111303011036	24.360107326886762
25	28.527602767914857	21.49321470270555	25.1915956126956	24.787586916683992
26	28.672420213666328	21.1046882783369	23.94460538470095	26.278286123295818
27	29.54501953660752	19.97293524574675	24.76894380406093	25.7131014135848
28	30.891253074336504	20.28114722734526	23.97748705979623	24.850112638522006
29	31.48145390744139	20.557729079265577	23.34296218813244	24.617854825160595
30	28.823500369661854	22.312952038952297	23.318944748208093	25.544602843177756
31	29.599824406378133	19.79559489592735	26.328762474936596	24.275818222757916
32	27.960771799836508	20.308056299637546	25.173632079249714	26.557539821276237
33	26.4291743831866	20.671842753104528	26.816450069167836	26.082532794541034
34	27.27667637974354	20.793861145512942	26.143412144993082	25.786050329750438
35	27.72428149166434	20.816944696353946	24.168067051195273	27.290706760786442
36	28.099656006042157	21.638110440859755	24.6513392017889	25.610894351309188
37	25.57586563634126	22.89132018481989	25.03045403287239	26.502360145966463
38	27.169149280317217	22.20372063180135	22.641919726811825	27.985210361069612
39	28.660581916926752	22.014627271777805	24.280809979749147	25.043980831546293
40	27.62172683354327	21.939401348620816	24.428882848010243	26.009988969825677
41	25.72851850795067	23.55463723525895	23.74908050048611	26.96776375630427
42	28.011364197000088	22.08138749769178	23.402253386916936	26.504994918391194
43	25.092111957832874	23.623251373461464	24.94954923023273	26.335087438472925
44	25.040955544848227	23.350203419181177	23.338755429643772	28.270085606326827
45	27.160970714203202	21.549999521750422	25.448910804982333	25.840118959064046
46	25.801910628774937	22.69262699424886	24.4436105830546	27.061851793921605
47	29.620087108807887	20.321075383623803	24.089117258351184	25.969720249217126
48	24.134483511225863	25.044580353764527	22.33602006806903	28.48491606694058
49	26.009026107515794	24.81630053584588	21.949673039611454	27.225000317026883
50	25.662609424268485	23.57004255694472	25.329926572732685	25.43742144605411
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1062.0
1	982.5
2	803.5
3	678.0
4	631.5
5	566.5
6	453.0
7	393.5
8	429.0
9	485.0
10	539.5
11	558.0
12	548.0
13	573.5
14	632.5
15	687.5
16	706.5
17	792.0
18	962.0
19	1137.5
20	1295.0
21	1629.5
22	2077.0
23	2594.0
24	3251.5
25	4118.5
26	5029.5
27	5973.0
28	6973.0
29	7873.0
30	9175.0
31	11958.5
32	15495.0
33	17837.5
34	19949.5
35	23373.5
36	27193.0
37	30242.5
38	33060.0
39	38704.0
40	46184.0
41	55777.0
42	69887.5
43	83730.0
44	89126.0
45	88164.5
46	88747.5
47	90783.5
48	98425.0
49	115396.5
50	135757.0
51	147648.5
52	154409.0
53	158487.5
54	151927.5
55	139432.5
56	130793.5
57	132303.5
58	137899.0
59	132983.0
60	121304.5
61	114617.0
62	111456.0
63	106639.0
64	98672.0
65	91510.0
66	82495.5
67	72754.0
68	65399.5
69	58732.0
70	52721.5
71	47716.5
72	42648.5
73	36294.0
74	29611.0
75	24450.5
76	20823.0
77	16783.0
78	13284.5
79	10301.0
80	7545.0
81	5618.5
82	4203.5
83	3100.0
84	2299.5
85	1702.5
86	1215.0
87	801.0
88	535.5
89	388.0
90	248.0
91	134.5
92	98.5
93	91.0
94	67.5
95	32.5
96	18.5
97	17.0
98	8.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	16793.0
25	15103.0
26	13658.0
27	14956.0
28	13732.0
29	11711.0
30	14632.0
31	12325.0
32	15349.0
33	13152.0
34	14121.0
35	14517.0
36	12930.0
37	13123.0
38	15456.0
39	18500.0
40	19083.0
41	18081.0
42	19458.0
43	19229.0
44	21032.0
45	29299.0
46	101016.0
47	179579.0
48	339510.0
49	346911.0
50	362808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.64684113707582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.56220390440108	15.871445852146932
2	13.970466807568135	7.445376192641179
3	7.383893574558639	5.90272317163017
4	4.4667488321121365	4.760989861140443
5	3.063577477895224	4.081733118229872
6	2.2109846936406616	3.534945473276938
7	1.6458291162826302	3.069930290025
8	1.2171907675610831	2.5947431213372454
9	0.9788252735795483	2.34743414094261
>10	4.994815122848475	22.59540182677115
>50	0.2824505022512667	5.274151323787506
>100	0.18750057788666136	9.871427081070802
>500	0.020860988467263628	3.818362382616516
>1k	0.013410635443240903	6.786890974634048
>5k	0.0012417255040037872	2.0444451897496467
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	6613	0.3922152421260403	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	6522	0.3868180567285702	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	6254	0.3709230491843726	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	5901	0.3499867146205601	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	5604	0.3323717249167291	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	4613	0.2735957828409835	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	3868	0.22941003425729986	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	3642	0.21600603535808843	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	3252	0.19287524079750235	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	3201	0.18985044458573339	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	3175	0.18830839161502766	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	3031	0.17976779054650358	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	2858	0.16950720731834618	No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC	2540	0.15064671329202214	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	2516	0.14922327978060143	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	2501	0.1483336338359635	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	2463	0.14607986410954743	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	2314	0.13724271439281072	No Hit
ATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	2278	0.1351075641256797	Illumina PCR Primer Index 11 (96% over 26bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	2269	0.13457377655889693	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	2191	0.1299476176467797	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	2164	0.12834625494643145	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	2119	0.12567731711251767	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	2113	0.1253214587346625	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	2100	0.12455043224930964	No Hit
ATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	1910	0.11328158361722923	Illumina PCR Primer Index 11 (96% over 26bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	1902	0.11280710578008901	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	1867	0.11073126524260052	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	1769	0.10491891173763272	No Hit
ATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	1722	0.1021313544444339	Illumina PCR Primer Index 11 (96% over 26bp)
AGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGG	1717	0.10183480579622126	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1186194592850568	0.0	0.0	0.0	0.0
2	0.3095967887339982	0.0	0.0	0.0	0.0
3	0.40579717021417927	0.0	0.0	0.0	0.0
4	0.5241200808510235	0.0	0.0	0.0	0.0
5	0.6805198379183708	0.0	0.0	0.0	0.0
6	0.8243459323015022	0.0	0.0	0.0	0.0
7	1.0429615957638618	0.0	0.0	0.0	0.0
8	1.134120650224428	0.0	0.0	0.0	0.0
9	1.2157308382125471	0.0	0.0	0.0	0.0
10	1.281386708926826	0.0	0.0	0.0	0.0
11	1.3460936239668244	0.0	0.0	0.0	0.0
12	1.4456746600366297	0.0	0.0	0.0	0.0
13	1.4784729405289478	0.0	0.0	0.0	0.0
14	1.5156601410148132	0.0	0.0	0.0	0.0
15	1.5629299955399083	0.0	0.0	0.0	0.0
16	1.5923476214426024	0.0	0.0	0.0	0.0
17	1.6294755121988251	0.0	0.0	0.0	0.0
18	1.6758557207792824	0.0	0.0	0.0	0.0
19	1.7627444747055865	0.0	0.0	0.0	0.0
20	1.7830284022433311	0.0	0.0	0.0	0.0
21	1.83272995568377	0.0	0.0	0.0	0.0
22	1.846193264312624	0.0	0.0	0.0	0.0
23	1.8629779178014596	0.0	0.0	0.0	0.0
24	1.8747805540003226	0.0	0.0	0.0	0.0
25	1.8920396853262984	0.0	0.0	0.0	0.0
26	1.9820718549236565	0.0	0.0	0.0	0.0
27	1.9945268981485875	0.0	0.0	0.0	0.0
28	2.0088798527220795	0.0	0.0	0.0	0.0
29	2.020208011083802	0.0	0.0	0.0	0.0
30	2.032840983497661	0.0	0.0	0.0	0.0
31	2.043635354292601	0.0	0.0	0.0	0.0
32	2.0544890348171836	0.0	0.0	0.0	0.0
33	2.065639263989979	0.0	0.0	0.0	0.0
34	2.076611563973847	0.0	0.0	0.0	0.0
35	2.0903714212509135	0.0	0.0	0.0	0.0
36	2.100394765560501	0.0	0.0	0.0	0.0
37	2.1087574374400972	0.0	0.0	0.0	0.0
38	2.115459436889703	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	1305	0.0	120.347786	44
GACCGTA	50	5.260554E-7	92.93128	44
AATGAAG	995	0.0	84.99242	44
CGTGCGC	635	0.0	83.418625	44
CAATGAA	1345	0.0	76.80219	43
TCGTGCG	1225	0.0	72.72122	43
CCAGAAT	755	0.0	72.62179	44
CTATGCG	40	0.0022620617	69.698456	44
CATGACC	560	0.0	68.03896	44
AGGTCCC	890	0.0	62.292427	43
ATAAGAT	855	0.0	57.6377	43
CCTATCA	1425	0.0	56.196754	43
CTATACG	50	0.0054753763	55.758766	44
TACATAT	50	0.0054753763	55.758766	44
TGCACCG	120	2.789966E-8	54.20991	44
TCCAGAA	1810	0.0	52.88253	43
CCCTATC	1315	0.0	52.88015	42
ATGTTCT	545	0.0	52.859993	44
GCAATGA	1625	0.0	52.214394	42
GGTCCCA	535	0.0	52.110992	44
>>END_MODULE