FastQCFastQC Report
Tue 24 May 2016
ERR841195.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841195.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3651171
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG341120.9342756063739551No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC270600.7411320915947239No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA263820.7225627065946788No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT166250.45533337112942673No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG153060.4192079746470379No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA148940.4079239235850635No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA145070.397324584359374No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC138840.3802615653991555No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA132800.36371892743451345No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC127830.35010685612917064No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG116960.32033558548750524No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA116550.3192126580759981No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC113990.3122012088724412No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG111210.3045872132529536No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT102930.28190955723519934No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC101290.2774178475891707No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC96520.26435354575285575No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT93750.25676693860681954No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT90300.2473179152660886No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG84620.23176126234569677No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT84550.231569543031537No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG81840.2241472667262092No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG80020.21916256455805547No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC71790.19662185090755815No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG70080.19193842194736976Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC67410.18462569953584754No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG64210.17586138803140144No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG63130.17290343289865087No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG62910.1723008864827202No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC61750.1691238235623585No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT61600.16871299646058757No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT61560.168603442566782No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC61300.16789134225704574No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA56260.15408755163754312No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA55050.15077354634992446No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG52420.1435703778322078No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC52310.14326910462424247No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG51920.1422009541596381No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG46530.12743856696933667No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC45440.12445322336313473No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTC44690.12239908785428018No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC44530.12196087227905787No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG43900.12023539845162005No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC41150.11270356825248667No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT40710.11149847542062533No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA40690.11144369847372254Illumina Single End Adapter 1 (100% over 32bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA40470.11084115205779188No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG40230.11018382869495841No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG39820.10906090128345125No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG38820.10632205393831184No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT38010.10410358758874894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA34550.0284.4569444
ACTTCTA7050.0176.0639844
CGTGCGC15300.0156.6983544
CTATCAT8450.0152.9303444
GAACACA11900.0135.7417444
AATGAAG12000.0114.7732144
ACATTGG6950.0112.5407544
TCGCCCC3550.0110.16312444
GACCGTA701.5583008E-597.162544
TCGTGCG49700.095.5188943
TTAAGCC32800.095.4699843
AATCGCA1304.4201443E-1091.55698444
CCCATAC4150.090.1387144
CATGACC4650.087.7596844
ATTAGTC3600.085.017244
ATGTTCT5800.085.017244
CAATGAA23550.082.2926643
CCAGAAT10450.076.4747944
AGGTCCC18100.068.2689643
GGAATCA13350.067.5042944
Produced by FastQC (version 0.11.5)