FastQCFastQC Report
Tue 24 May 2016
ERR841194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334243
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC312880.7218792301216153No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG163860.3780590982093067No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC140700.3246241615894633No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA117810.27181217112192374No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT112780.2602069150252997No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG110430.25478497629228447No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG105840.24419489170311864No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG97460.22486048890198357No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC77480.1787624736314969No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT76240.17590153574684206No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG75110.17329439073905178No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG72750.16784937992632162No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG70840.1634426127007646No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA64550.1489302745600558No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG57950.13370270194818334No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC57140.1318338634912717No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA54790.12641192475825652No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC53970.12452001422162993No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG52610.12138221138039561No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG51890.1197210216409186No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG49960.11526811025593166No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT49790.11487588490077738No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT48340.11153043334210841No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT47390.10933858576918738No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC45500.10497796270306026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTCT9550.0122.586744
AATCGCA1206.311893E-1087.55257444
TCGTGCG26100.076.9012543
CGTGCGC14600.070.9330144
CGCTACC5550.067.6081644
ATCCTTG18800.066.4353443
AATCCCG20750.064.1735943
ACGCTAC9050.057.99613643
TGTGTTA24750.056.6285342
ACGACAC2455.2750693E-1155.13514744
TAATCCC58350.053.11176742
CTCGTGC29800.052.05492842
CTATCAT4600.048.94243244
ACGAACA1851.2593682E-648.6778844
CTCGGAA6850.048.20412444
GATCCTT21100.047.72242742
CTAATCC74900.047.19724741
TAATCCT15100.046.63442642
CCAGAAT8700.046.57969744
GTGTTAG18700.045.220143
Produced by FastQC (version 0.11.5)