##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841194.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4334243
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.248034316488486	37.0	34.0	39.0	29.0	39.0
2	36.34581171383331	37.0	35.0	39.0	32.0	39.0
3	36.61765895451639	38.0	35.0	39.0	32.0	39.0
4	37.137035233142214	38.0	35.0	40.0	32.0	41.0
5	37.48845253946306	39.0	36.0	40.0	33.0	41.0
6	37.48672397925082	39.0	36.0	40.0	33.0	41.0
7	37.44168312667287	39.0	36.0	40.0	33.0	41.0
8	37.297078174896974	38.0	36.0	40.0	32.0	41.0
9	37.21642441367501	38.0	35.0	40.0	32.0	41.0
10	37.021032277147356	38.0	35.0	40.0	31.0	41.0
11	36.890971733702976	38.0	35.0	40.0	31.0	41.0
12	36.75644212841781	38.0	35.0	40.0	31.0	41.0
13	36.56672480061685	38.0	35.0	40.0	31.0	41.0
14	36.51057681814333	38.0	35.0	40.0	31.0	41.0
15	36.47278313652465	38.0	35.0	40.0	31.0	41.0
16	36.448549377596045	38.0	35.0	40.0	31.0	41.0
17	36.41058911556182	38.0	35.0	40.0	31.0	41.0
18	36.29337902835628	38.0	34.0	40.0	30.0	41.0
19	36.17679650171898	38.0	34.0	40.0	30.0	41.0
20	36.07591913974367	38.0	34.0	40.0	30.0	41.0
21	35.88210836355968	37.0	34.0	40.0	30.0	41.0
22	35.9420288617874	37.0	34.0	40.0	30.0	41.0
23	35.962456189004634	37.0	34.0	40.0	30.0	41.0
24	35.861673191835344	37.0	34.0	40.0	30.0	41.0
25	35.82988764648147	37.0	34.0	40.0	30.0	41.0
26	35.770329338349484	37.0	34.0	40.0	30.0	41.0
27	35.74752426495748	37.0	34.0	40.0	30.0	41.0
28	35.72230179958307	37.0	34.0	39.0	30.0	41.0
29	35.733444555807374	37.0	34.0	39.0	30.0	41.0
30	35.68866535061368	36.0	34.0	39.0	30.0	41.0
31	35.55960021096366	36.0	34.0	39.0	30.0	41.0
32	35.49395511921458	36.0	34.0	39.0	30.0	41.0
33	35.388231262889086	36.0	34.0	39.0	30.0	40.0
34	35.30068898392507	36.0	33.0	39.0	30.0	40.0
35	35.16227856919868	35.0	33.0	39.0	30.0	40.0
36	34.99374322613955	35.0	33.0	39.0	29.0	40.0
37	34.940914885698675	35.0	33.0	38.0	29.0	40.0
38	34.85749899361435	35.0	33.0	38.0	29.0	40.0
39	34.677078700445186	35.0	33.0	38.0	29.0	40.0
40	34.535660085655806	35.0	33.0	38.0	29.0	40.0
41	34.3917869436928	35.0	33.0	37.0	29.0	40.0
42	34.26007940199391	35.0	33.0	37.0	29.0	40.0
43	34.14852970890143	35.0	33.0	37.0	29.0	39.0
44	34.06700079690987	35.0	33.0	36.0	29.0	39.0
45	34.014259524578904	35.0	33.0	36.0	29.0	39.0
46	33.92674421147975	35.0	33.0	36.0	29.0	39.0
47	33.89135700383414	35.0	33.0	36.0	29.0	39.0
48	33.904709486225165	35.0	33.0	36.0	29.0	39.0
49	33.891287853447984	35.0	33.0	36.0	29.0	39.0
50	34.526171152932065	35.0	33.0	37.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	23.0
22	91.0
23	267.0
24	885.0
25	2359.0
26	6120.0
27	14721.0
28	32314.0
29	65206.0
30	117765.0
31	193556.0
32	290004.0
33	403061.0
34	525841.0
35	535260.0
36	608559.0
37	610130.0
38	499195.0
39	337820.0
40	91061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.819927493682286	19.071842533978828	35.10822997233888
2	28.580930972259743	21.095610006176397	27.542272087651753	22.781186933912103
3	27.482746121987162	16.62405638077976	27.805985958793727	28.087211538439355
4	20.488191363520688	18.04395831059772	31.237842455995203	30.23000786988639
5	23.479255777767882	19.194078412308677	28.34614948908033	28.98051632084311
6	27.70084649153266	19.241999121876646	25.566102315906146	27.491052070684546
7	31.5509536498069	19.80590382218994	21.605318391239255	27.037824136763906
8	28.313364063805373	17.414667336372233	27.04601472506272	27.22595387475967
9	28.995028658983816	17.34948871117748	24.23311291037443	29.422369719464275
10	27.54686343151503	20.587447450454437	25.83572263945515	26.029966478575382
11	34.04052333936976	16.688427483184494	24.291116118777836	24.979933058667918
12	31.298545097725256	17.300137532667183	22.96760933800897	28.4337080315986
13	28.902371186848548	18.535970410519205	25.815257704747978	26.746400697884265
14	28.02932369043452	19.067597271311275	21.777989835826002	31.125089202428196
15	26.353852333613965	20.80056425078151	22.80435130194592	30.0412321136586
16	28.379511716348162	21.117505409825892	24.802301116942452	25.700681756883498
17	27.613772462688406	21.20582533097475	19.082155753611417	32.09824645272543
18	27.933736064175452	19.881949396930445	22.122294481412325	30.06202005748178
19	28.33311376404138	18.20084845265944	26.509750376247943	26.956287407051242
20	28.371067335172484	18.476859742289484	24.682349374504383	28.46972354803365
21	30.34179209610536	20.80783195589172	21.24696284910652	27.603413098896397
22	29.666472322848534	19.776556136792514	24.826457584404015	25.730513955954937
23	29.449179476093057	21.967111673249516	22.58246711132717	26.00124173933026
24	32.86622369811752	22.151388373932885	20.29187103722611	24.690516890723476
25	30.023749808892813	22.212315122034337	22.047361903906058	25.71657316516679
26	31.338476934042642	21.972843865205878	21.718983804502262	24.969695396249215
27	29.43949468322919	22.127852868695612	23.184506671149467	25.24814577692573
28	29.94988914433396	21.491933007289617	23.893279894599807	24.664897953776617
29	31.227390969198606	20.418472330229136	24.51940198513398	23.83473471543828
30	29.512561766617214	22.146817563176675	22.75245533798448	25.588165332221635
31	28.205771930024763	21.30594047272332	26.37901616114607	24.109271436105846
32	28.288744740532962	19.808505309557205	25.64586255259467	26.256887397315165
33	26.90649608516051	19.446448604493025	26.45170710992754	27.195348200418927
34	26.953799533179907	18.605045255784898	29.817545524160444	24.623609686874747
35	30.221077689504845	19.55198825835442	24.000680660137046	26.22625339200369
36	28.145579602070647	20.800990381510093	22.79027135157394	28.26315866484532
37	25.95446423151132	21.0748098937094	27.319375928756003	25.651349946023274
38	30.40655845986849	21.17416727423282	22.47637294611295	25.942901319785744
39	27.860668992646392	21.016696748953954	25.307956621053858	25.81467763734579
40	27.584985555045105	20.682480733455535	23.172817740498044	28.55971597100132
41	26.081496578977532	22.231579716717082	23.530167399485833	28.156756304819552
42	26.29478495199804	21.711973643385626	27.038168726992808	24.955072677623527
43	25.00035572706343	24.233835050783593	24.645254743264662	26.12055447888831
44	24.218896902276434	23.41017346870692	24.421576009864467	27.949353619152177
45	26.600498140622975	20.11516936136073	26.795672615930293	26.488659882085997
46	25.46736175551182	21.660273179623974	24.06122406787573	28.811140996988478
47	27.271229147245702	21.234640372466497	24.181004052562397	27.313126427725404
48	23.854837904988077	22.737484908051368	24.9103662978078	28.497310889152754
49	27.775709869184297	22.189669338557042	22.66252475057878	27.37209604167989
50	24.976321673151066	24.851856416034366	24.218615213985174	25.953206696829394
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1720.0
1	1758.5
2	2059.5
3	2298.0
4	2210.0
5	1833.5
6	1360.0
7	1259.0
8	1364.0
9	1457.0
10	1534.0
11	1576.0
12	1570.5
13	1570.5
14	1662.5
15	1861.0
16	2086.5
17	2471.0
18	3005.5
19	3672.5
20	4370.5
21	5493.5
22	6831.5
23	8407.5
24	10169.0
25	11903.5
26	14304.0
27	17258.5
28	20478.0
29	24045.5
30	28274.5
31	33413.5
32	39651.0
33	45845.0
34	52644.5
35	60662.5
36	68989.5
37	76436.0
38	83556.0
39	95917.0
40	110982.0
41	131335.0
42	161129.5
43	180202.0
44	188059.5
45	196061.5
46	212996.5
47	233055.0
48	256072.0
49	284696.0
50	308440.0
51	327582.5
52	356941.0
53	386426.0
54	382192.0
55	376146.5
56	383483.0
57	394723.0
58	394121.0
59	359292.5
60	328264.5
61	317104.5
62	303937.0
63	285025.5
64	261771.5
65	241290.5
66	221299.5
67	202880.0
68	182479.5
69	160246.0
70	142654.0
71	127870.5
72	116430.5
73	105003.0
74	90192.5
75	76815.0
76	67354.0
77	54546.0
78	43177.0
79	34690.5
80	26203.0
81	19023.0
82	14086.5
83	10715.5
84	8072.0
85	5995.5
86	4412.5
87	3167.5
88	2178.0
89	1526.5
90	1006.5
91	590.5
92	417.0
93	351.5
94	252.0
95	126.0
96	80.5
97	77.0
98	45.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	49998.0
25	47265.0
26	43175.0
27	42010.0
28	38118.0
29	35827.0
30	46824.0
31	38226.0
32	41297.0
33	39075.0
34	39717.0
35	45499.0
36	38542.0
37	40107.0
38	43171.0
39	46329.0
40	46985.0
41	47432.0
42	50716.0
43	51807.0
44	51841.0
45	72771.0
46	260939.0
47	571097.0
48	756526.0
49	1180919.0
50	568030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.74688027492254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.82173302886711	13.152640384961742
2	12.76052926817212	5.805244630155123
3	6.817839466483027	4.652537342331935
4	4.656294865369224	4.236647273091613
5	3.271110653553449	3.7203781201201958
6	2.5265330104116863	3.4482446339084554
7	1.9506005378989402	3.1059053829850694
8	1.5491913052549207	2.819141531486773
9	1.2440457100856614	2.5468342941464583
>10	6.8046259950496	26.31851821213786
>50	0.33252185656860084	5.22033812439294
>100	0.22630003510464977	10.218585513658605
>500	0.021115260818254357	3.307642042033674
>1k	0.015336347329456126	6.575823128275517
>5k	0.001444728371615432	2.1810456733109405
>10k+	7.779306616390788E-4	2.6904737130031227
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	31288	0.7218792301216153	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	16386	0.3780590982093067	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	14070	0.3246241615894633	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	11781	0.27181217112192374	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	11278	0.2602069150252997	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	11043	0.25478497629228447	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	10584	0.24419489170311864	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	9746	0.22486048890198357	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	7748	0.1787624736314969	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	7624	0.17590153574684206	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	7511	0.17329439073905178	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	7275	0.16784937992632162	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	7084	0.1634426127007646	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	6455	0.1489302745600558	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	5795	0.13370270194818334	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	5714	0.1318338634912717	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA	5479	0.12641192475825652	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	5397	0.12452001422162993	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	5261	0.12138221138039561	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	5189	0.1197210216409186	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	4996	0.11526811025593166	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	4979	0.11487588490077738	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT	4834	0.11153043334210841	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	4739	0.10933858576918738	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	4550	0.10497796270306026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1563594842282724	0.0	0.0	0.0	0.0
2	0.452581915688622	0.0	0.0	0.0	0.0
3	0.6120561306784137	0.0	0.0	0.0	0.0
4	0.7261014207094526	0.0	0.0	0.0	0.0
5	0.881907175024566	0.0	0.0	0.0	0.0
6	1.0193014097271427	0.0	0.0	0.0	0.0
7	1.1326776094464477	0.0	0.0	0.0	0.0
8	1.220974458515593	0.0	0.0	0.0	0.0
9	1.299627178263886	0.0	0.0	0.0	0.0
10	1.3705968954670977	0.0	0.0	0.0	2.30720797149583E-5
11	1.435683232342995	0.0	0.0	0.0	2.30720797149583E-5
12	1.487133970107352	0.0	0.0	0.0	2.30720797149583E-5
13	1.5269102355359403	0.0	0.0	0.0	2.30720797149583E-5
14	1.569593583008613	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
15	1.6158069586776744	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
16	1.6498844204166678	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
17	1.6832697197642126	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
18	1.7106332063061531	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
19	1.759407582823575	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
20	1.7806108240816216	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
21	1.8121965012113996	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
22	1.8288776148453143	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
23	1.8469430532621267	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
24	1.8629781486640227	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
25	1.8809051546025453	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
26	1.9151902650589734	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
27	1.9307869909462851	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
28	1.9445610225361154	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
29	1.9584273424448053	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
30	1.9723398065129252	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
31	1.9861369101824702	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
32	1.9993802839388561	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
33	2.012600585615527	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
34	2.0247826437050254	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
35	2.038302882417991	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
36	2.0505772288263486	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
37	2.06091352053865	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
38	2.0678812886125675	2.3072079714958298E-4	0.0	0.0	2.30720797149583E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTTCT	955	0.0	122.5867	44
AATCGCA	120	6.311893E-10	87.552574	44
TCGTGCG	2610	0.0	76.90125	43
CGTGCGC	1460	0.0	70.93301	44
CGCTACC	555	0.0	67.60816	44
ATCCTTG	1880	0.0	66.43534	43
AATCCCG	2075	0.0	64.17359	43
ACGCTAC	905	0.0	57.996136	43
TGTGTTA	2475	0.0	56.62853	42
ACGACAC	245	5.2750693E-11	55.135147	44
TAATCCC	5835	0.0	53.111767	42
CTCGTGC	2980	0.0	52.054928	42
CTATCAT	460	0.0	48.942432	44
ACGAACA	185	1.2593682E-6	48.67788	44
CTCGGAA	685	0.0	48.204124	44
GATCCTT	2110	0.0	47.722427	42
CTAATCC	7490	0.0	47.197247	41
TAATCCT	1510	0.0	46.634426	42
CCAGAAT	870	0.0	46.579697	44
GTGTTAG	1870	0.0	45.2201	43
>>END_MODULE