FastQCFastQC Report
Tue 24 May 2016
ERR841192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4054399
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA200390.49425327896933674No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC161310.39786414706594003No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG150180.3704124828365437No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG146760.36197720056659444No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC139430.34389807219269736No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC136440.33652336634850194No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA95180.23475735861221356No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA88290.2177634712321111No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC87640.21616027430945992No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG86410.21312653244044305No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC85900.21186863947036294No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT83580.20614645968490028No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA74920.18478694376157848No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG71740.1769436111246081No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA71150.17548840161020166No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT70440.17373721727930577No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC69800.17215868492469538No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC68910.1699635383690653No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG66270.16345209240629746No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA66110.16305745931764487No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC66060.16293413647744093No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT65770.16221886400425808No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAG64660.1594810969517307No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA60930.15028121307251704No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT54450.13429857298208686No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT53630.1322760784027423No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG52750.13010559641515304Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG44170.10894339703615753No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG42220.10413380626820398No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC41810.10312255897853172No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT41150.10149469748783975No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG41040.1012233872393911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTC18450.0142.681744
TAAGCCA27000.0126.52804644
CTATACG1350.0119.90188644
TATTAGT17900.080.40176443
TTAAGCC25600.074.1302843
TCGTGCG22750.071.44321443
CTATGCG1650.067.122144
GAACACA20900.060.73599644
ATTAAGC35000.055.0782342
ATATTAG25300.054.83089442
AGGTCCC15700.050.0271543
AATCCCG8700.049.05326543
CAATGAA19400.047.74058543
CTCGTGC24900.047.55571742
CATATTA25600.047.34611541
GTTCAGG37350.046.55512243
TGTGTTA18750.046.4906742
AATCGCA1301.0416625E-745.87339444
ACGCTAC9150.044.6560943
CTAATCC47150.044.3195641
Produced by FastQC (version 0.11.5)