##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841192.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4054399
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.065709122363145	37.0	34.0	39.0	27.0	39.0
2	35.90299252737582	37.0	35.0	39.0	31.0	39.0
3	36.17617974945239	37.0	35.0	39.0	32.0	39.0
4	36.620714438810786	38.0	35.0	39.0	32.0	40.0
5	37.16656969380665	38.0	35.0	40.0	33.0	41.0
6	37.24738709732318	38.0	35.0	40.0	33.0	41.0
7	37.21615509475017	38.0	35.0	40.0	33.0	41.0
8	37.10125964415441	38.0	35.0	40.0	32.0	41.0
9	37.048673551863054	38.0	35.0	40.0	32.0	41.0
10	36.9624368494566	38.0	35.0	40.0	32.0	41.0
11	36.836029705019165	38.0	35.0	40.0	32.0	41.0
12	36.76945263650667	38.0	35.0	40.0	32.0	41.0
13	36.65338561892897	38.0	35.0	40.0	31.0	41.0
14	36.60141194786206	38.0	35.0	40.0	31.0	41.0
15	36.565010005182025	38.0	35.0	40.0	31.0	41.0
16	36.54205321183238	38.0	35.0	40.0	31.0	41.0
17	36.538806614741176	38.0	35.0	40.0	31.0	41.0
18	36.41833746505956	38.0	35.0	40.0	31.0	41.0
19	36.28543515327426	38.0	34.0	40.0	31.0	41.0
20	36.187602157557755	38.0	34.0	40.0	31.0	41.0
21	36.05950943654041	37.0	34.0	40.0	30.0	41.0
22	36.088255990591946	37.0	34.0	40.0	30.0	41.0
23	36.09732293244942	37.0	34.0	40.0	30.0	41.0
24	36.08823083273255	37.0	34.0	40.0	30.0	41.0
25	36.06440521126501	37.0	34.0	40.0	30.0	41.0
26	36.05303787895733	37.0	34.0	40.0	31.0	41.0
27	36.096342236122446	37.0	34.0	40.0	31.0	41.0
28	36.09462398620443	37.0	34.0	40.0	31.0	41.0
29	36.12422518592834	37.0	34.0	40.0	31.0	41.0
30	36.14781649575494	37.0	34.0	40.0	31.0	41.0
31	36.09162686250749	37.0	34.0	40.0	31.0	41.0
32	36.10114108116274	37.0	34.0	40.0	31.0	41.0
33	36.044470400592445	37.0	34.0	40.0	31.0	41.0
34	36.00234315223144	36.0	34.0	40.0	31.0	41.0
35	35.940828483936514	36.0	34.0	40.0	31.0	41.0
36	35.84946348015321	36.0	34.0	40.0	31.0	41.0
37	35.79762072410257	36.0	34.0	40.0	31.0	41.0
38	35.71281706507673	36.0	34.0	39.0	31.0	41.0
39	35.613256187850595	35.0	34.0	39.0	30.0	41.0
40	35.48081630804846	35.0	34.0	39.0	30.0	41.0
41	35.371392496289324	35.0	34.0	39.0	30.0	41.0
42	35.259471129897754	35.0	34.0	39.0	30.0	41.0
43	35.166848086575925	35.0	33.0	39.0	30.0	40.0
44	35.02495417194597	35.0	33.0	39.0	30.0	40.0
45	34.976532968961905	35.0	33.0	38.0	30.0	40.0
46	34.92198458374327	35.0	33.0	38.0	30.0	40.0
47	34.95869381885734	35.0	33.0	38.0	30.0	40.0
48	35.01760008693494	35.0	33.0	38.0	30.0	40.0
49	35.120417110793795	35.0	33.0	39.0	30.0	40.0
50	35.78725887553023	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	56.0
22	201.0
23	618.0
24	1727.0
25	4099.0
26	8941.0
27	17840.0
28	34103.0
29	61872.0
30	105196.0
31	167955.0
32	253773.0
33	373080.0
34	543456.0
35	355023.0
36	417614.0
37	485742.0
38	500569.0
39	476424.0
40	246099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.244684847248635	13.886867079436435	35.86844807331494
2	27.53219404404944	25.09836846348867	28.656553042756766	18.712884449705122
3	31.00935551730355	18.31748675944326	22.90731622615337	27.76584149709982
4	21.501707158076943	20.273855631870468	26.96330578218868	31.26113142786391
5	23.246404707578115	21.917181806724006	26.425149572106744	28.41126391359114
6	25.527482618262287	22.787150450658654	24.601352752898762	27.084014178180293
7	31.104536085372946	22.888990452099065	21.29457411567041	24.711899346857574
8	27.946583451702704	19.19643330614476	26.760982330550103	26.096000911602435
9	26.041590874504454	23.17292895938461	24.845162007981948	25.94031815812898
10	28.739869953598546	21.661015603052387	24.31302395250196	25.2860904908471
11	30.61913738633025	21.6031525264287	23.523017838155543	24.2546922490855
12	29.52573242051411	20.818572617051256	22.674655355824626	26.981039606610008
13	26.412743294382224	20.664641047908702	25.315687972496047	27.606927685213023
14	24.89002685724814	21.377965020216312	22.726648265254603	31.005359857280943
15	23.648017868986255	22.02171024608086	25.670561777466894	28.65971010746599
16	25.93891227775066	25.667898004118488	24.128730300101196	24.264459418029652
17	28.050495276858545	24.09987275549348	19.110551280226737	28.739080687421243
18	26.358826548645066	23.072322186346238	21.942536982669935	28.62631428233876
19	26.279973924618666	22.08749064904564	25.507997609510063	26.124537816825626
20	28.094398207971143	19.781427530936153	24.88124627102562	27.242927990067084
21	29.189233718733654	23.02923318597898	22.367606148284864	25.4139269470025
22	28.4702615603447	22.936543739281703	23.019046719378135	25.57414798099546
23	28.264411075476293	22.937850961387866	21.984738058587723	26.812999904548125
24	30.155147532346966	22.744308095971807	20.81048263873388	26.29006173294735
25	27.072172105817028	23.90149117402874	23.209866520974103	25.81647019918013
26	28.523005415300815	22.934189782078565	23.195894555067955	25.34691024755266
27	27.0090328925025	22.455127002874104	22.980347629499338	27.555492475124062
28	27.39443838083013	21.735736074933154	24.508904739185223	26.360920805051492
29	31.385676957864483	21.145062601861124	24.033933579529638	23.435326860744755
30	26.763088875224366	24.28705805587246	22.600961801545637	26.348891267357537
31	25.284980331292743	22.988858325110776	27.1601161016439	24.56604524195258
32	26.254252064968576	21.17745192197423	25.308153603976848	27.260142409080352
33	25.84863481065253	22.180828934502195	26.175793997444224	25.794742257401047
34	24.062243131266392	22.855224827333274	27.79632698823437	25.28620505316596
35	27.239234717473586	21.50531908711237	25.21528672956895	26.040159465845104
36	26.2277982146374	21.766382963169466	25.042320938649233	26.96349788354391
37	24.28790052501177	23.271542865692922	25.67571061421536	26.764845995079938
38	28.02855833198298	23.930768991718075	22.436426052000115	25.60424662429883
39	25.520953208151127	25.03016995414167	24.306427054230877	25.14244978347633
40	26.939948549159894	23.79106091179492	22.494262014393783	26.774728524651405
41	25.262749987845577	24.344018737827707	22.39199009343175	28.001241180894965
42	24.9765030461477	25.958937356586187	24.337437940143264	24.72712165712285
43	24.67436589352524	24.741579043776625	25.149334920464927	25.434720142233207
44	24.28102933440031	25.106778995413492	24.217692518579444	26.39449915160676
45	25.25825005530495	22.90652742644442	27.466991362810067	24.368231155440565
46	24.03838658973473	25.29342013442426	24.310064511614975	26.358128764226034
47	27.515504824454073	24.17436974237126	23.37416423930976	24.935961193864905
48	24.46182030054211	24.781814874815225	23.623722757687347	27.13264206695532
49	25.665352720739566	23.652646774240402	22.996902841743257	27.685097663276775
50	22.977018786604518	29.594643484461596	22.58052338232287	24.84781434661102
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5995.0
1	5796.5
2	4014.5
3	2401.0
4	2267.0
5	1828.0
6	1300.0
7	1163.0
8	1270.0
9	1341.0
10	1426.0
11	1521.0
12	1545.0
13	1528.0
14	1575.5
15	1779.0
16	1971.0
17	2250.0
18	2650.0
19	3436.0
20	4433.5
21	5669.5
22	7287.5
23	9641.5
24	12103.5
25	14496.5
26	17354.5
27	21522.0
28	26140.5
29	30752.0
30	36382.0
31	44551.5
32	54032.0
33	62579.0
34	77154.5
35	97527.0
36	107589.0
37	111102.5
38	117371.0
39	127549.0
40	141814.0
41	165638.5
42	199282.0
43	234303.5
44	253806.0
45	238538.0
46	228353.0
47	233553.5
48	250315.5
49	281694.5
50	313598.0
51	338501.0
52	344586.5
53	337576.5
54	321352.0
55	301169.0
56	286506.5
57	284392.5
58	282741.5
59	266898.0
60	249053.5
61	239874.5
62	238006.5
63	229527.0
64	210983.5
65	194524.5
66	170270.0
67	148724.5
68	135476.0
69	121891.5
70	110620.0
71	104201.0
72	99559.5
73	87876.0
74	70047.5
75	55452.5
76	46032.5
77	35945.5
78	28897.5
79	24618.0
80	19840.5
81	15313.5
82	11773.5
83	9530.5
84	7799.0
85	5967.0
86	4332.0
87	2710.0
88	1563.5
89	1080.0
90	761.5
91	511.5
92	369.5
93	292.0
94	180.5
95	78.5
96	59.0
97	56.0
98	35.5
99	14.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	38637.0
25	36851.0
26	33331.0
27	32439.0
28	29790.0
29	27462.0
30	35099.0
31	27633.0
32	33825.0
33	29314.0
34	30417.0
35	33567.0
36	30306.0
37	31736.0
38	32558.0
39	37964.0
40	36800.0
41	37711.0
42	41473.0
43	43461.0
44	47017.0
45	68370.0
46	218294.0
47	445300.0
48	805016.0
49	836423.0
50	953605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.223437775223402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.888135370090765	12.642499365456978
2	12.456030413739768	5.536316336520717
3	6.93404384291669	4.622948756211899
4	4.625557254491766	4.111831352837238
5	3.4465252058529585	3.8296819226506122
6	2.723970781395499	3.632159709712172
7	2.093284800929289	3.2563989163491067
8	1.6987746273548092	3.0202089780118335
9	1.3336495776004527	2.6674450561580967
>10	7.252400293033827	26.911555124050402
>50	0.31524975572608893	4.799289119548012
>100	0.18857685964293497	8.25441807958076
>500	0.02476781921109169	3.7295299148970744
>1k	0.015739156050121907	6.372198777785944
>5k	0.0025621881942058914	4.07963344498913
>10k+	7.320537697731119E-4	2.533885145240093
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	20039	0.49425327896933674	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	16131	0.39786414706594003	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	15018	0.3704124828365437	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	14676	0.36197720056659444	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	13943	0.34389807219269736	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	13644	0.33652336634850194	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	9518	0.23475735861221356	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	8829	0.2177634712321111	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	8764	0.21616027430945992	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	8641	0.21312653244044305	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	8590	0.21186863947036294	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	8358	0.20614645968490028	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	7492	0.18478694376157848	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	7174	0.1769436111246081	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	7115	0.17548840161020166	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	7044	0.17373721727930577	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	6980	0.17215868492469538	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	6891	0.1699635383690653	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	6627	0.16345209240629746	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	6611	0.16305745931764487	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	6606	0.16293413647744093	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	6577	0.16221886400425808	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAG	6466	0.1594810969517307	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	6093	0.15028121307251704	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	5445	0.13429857298208686	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	5363	0.1322760784027423	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	5275	0.13010559641515304	Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	4417	0.10894339703615753	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	4222	0.10413380626820398	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	4181	0.10312255897853172	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	4115	0.10149469748783975	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	4104	0.1012233872393911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.22092053594133187	0.0	0.0	0.0	0.0
2	0.5665697924649251	0.0	0.0	0.0	0.0
3	0.792719216830904	0.0	0.0	0.0	0.0
4	0.9375248957983662	0.0	0.0	0.0	0.0
5	1.107883067256084	0.0	0.0	0.0	0.0
6	1.2641824349305533	0.0	0.0	0.0	0.0
7	1.4110599376134416	0.0	0.0	0.0	0.0
8	1.5090276018714488	0.0	0.0	0.0	0.0
9	1.5942436844523689	0.0	0.0	0.0	0.0
10	1.6689279964798729	0.0	0.0	0.0	0.0
11	1.7343877600601223	0.0	0.0	0.0	0.0
12	1.78601070096949	0.0	0.0	0.0	0.0
13	1.820713748202878	0.0	0.0	0.0	0.0
14	1.8554661245723472	0.0	0.0	0.0	0.0
15	1.8910077671191217	0.0	0.0	0.0	0.0
16	1.9207285716082705	0.0	0.0	0.0	0.0
17	1.9486735271984825	0.0	0.0	0.0	0.0
18	1.9721048668372303	0.0	0.0	0.0	0.0
19	2.0191648626590526	0.0	0.0	0.0	0.0
20	2.0383045674587037	0.0	0.0	0.0	0.0
21	2.068617321580831	0.0	0.0	0.0	2.4664568040787303E-5
22	2.0851425821681584	0.0	0.0	0.0	2.4664568040787303E-5
23	2.1000893104008758	0.0	0.0	0.0	2.4664568040787303E-5
24	2.1136054936872273	0.0	0.0	0.0	2.4664568040787303E-5
25	2.1298841085941467	0.0	0.0	0.0	2.4664568040787303E-5
26	2.177758035161315	0.0	0.0	0.0	2.4664568040787303E-5
27	2.1915948578321967	0.0	0.0	0.0	2.4664568040787303E-5
28	2.2060976238401797	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
29	2.219490484286327	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
30	2.2315021289221906	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
31	2.244204381463196	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
32	2.2565613300516305	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
33	2.268745626663779	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
34	2.2813738855006624	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
35	2.293780163225178	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
36	2.306556409470306	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
37	2.316792205207233	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
38	2.3243888921637956	2.4664568040787303E-5	0.0	0.0	2.4664568040787303E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGTC	1845	0.0	142.6817	44
TAAGCCA	2700	0.0	126.528046	44
CTATACG	135	0.0	119.901886	44
TATTAGT	1790	0.0	80.401764	43
TTAAGCC	2560	0.0	74.13028	43
TCGTGCG	2275	0.0	71.443214	43
CTATGCG	165	0.0	67.1221	44
GAACACA	2090	0.0	60.735996	44
ATTAAGC	3500	0.0	55.07823	42
ATATTAG	2530	0.0	54.830894	42
AGGTCCC	1570	0.0	50.02715	43
AATCCCG	870	0.0	49.053265	43
CAATGAA	1940	0.0	47.740585	43
CTCGTGC	2490	0.0	47.555717	42
CATATTA	2560	0.0	47.346115	41
GTTCAGG	3735	0.0	46.555122	43
TGTGTTA	1875	0.0	46.49067	42
AATCGCA	130	1.0416625E-7	45.873394	44
ACGCTAC	915	0.0	44.65609	43
CTAATCC	4715	0.0	44.31956	41
>>END_MODULE