FastQCFastQC Report
Tue 24 May 2016
ERR841191.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841191.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3621797
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC294720.8137396988290619No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG162940.44988716926984035No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC138870.3834284472597443No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG103810.28662567228367575No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA102440.282843019639146No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT100970.2787842609621688No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG89360.2467283505950223No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG85110.23499384421600658No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG82630.22814641461131033No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT78200.21591491737388926No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT77620.21431350238569416No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG72980.2015021824801335No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT70450.19451669985921352No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA64220.17731529403773874No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC63890.17640414413066222No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC58380.1611907017428089No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG57040.15749088090801336No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT56310.1554753068711471No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG54010.14912486812485626No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG48870.13493301805705843No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG47670.13161974566768928No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC42820.11822860309398898No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC41070.11339674752615897No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC41070.11339674752615897No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG39740.10972453729460817No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC39530.10914471462646856No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGC37210.10273905467368823No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA37100.10243533803799607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCA1000.0265.96144
CTATCAT6400.0241.0271644
ATGTTCT7500.0234.0456844
ACTTCTA4900.0173.6888344
CCCATAC2900.0128.3949744
CGTGCGC12150.0118.20489544
ACATTGG4000.099.73537444
TCGTGCG24000.088.2033543
CGCTACC4000.086.43732544
ACGACAC1354.402209E-578.8032644
AATCCCG14600.065.67058643
TATGTTC17400.065.3186143
AACGACA3150.064.9795743
CAAAACG2155.9097056E-661.85140244
TCTTCTA4102.910383E-1158.38168344
TCGCCCC4153.274181E-1157.6782944
GTTCAGG27200.056.83511743
CCTATCA5650.056.24802843
TAATCCC51400.056.0514442
TGTGTTA14400.054.67519442
Produced by FastQC (version 0.11.5)