##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841191.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3621797
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.16260464073497	38.0	35.0	39.0	31.0	39.0
2	36.861546077817174	39.0	35.0	39.0	33.0	39.0
3	37.20806467065935	39.0	37.0	39.0	33.0	40.0
4	37.831242060225904	39.0	37.0	40.0	33.0	41.0
5	38.12356379995897	40.0	37.0	41.0	33.0	41.0
6	38.176372944148994	40.0	37.0	41.0	33.0	41.0
7	38.12767584710021	40.0	37.0	41.0	33.0	41.0
8	38.004642170723535	40.0	37.0	41.0	33.0	41.0
9	37.94684517105735	39.0	37.0	41.0	33.0	41.0
10	37.831734081175725	39.0	36.0	41.0	33.0	41.0
11	37.713847573455936	39.0	36.0	40.0	33.0	41.0
12	37.578896608506774	39.0	36.0	40.0	32.0	41.0
13	37.43190907717909	39.0	36.0	40.0	32.0	41.0
14	37.40267165719117	39.0	36.0	40.0	32.0	41.0
15	37.38471841464334	39.0	36.0	40.0	32.0	41.0
16	37.32221601597218	39.0	36.0	40.0	32.0	41.0
17	37.2790559492981	39.0	36.0	40.0	32.0	41.0
18	37.14767448313641	38.0	35.0	40.0	31.0	41.0
19	37.06526815279818	38.0	35.0	40.0	31.0	41.0
20	36.91626145805522	38.0	35.0	40.0	31.0	41.0
21	36.74658684625339	38.0	35.0	40.0	31.0	41.0
22	36.814180364056845	38.0	35.0	40.0	31.0	41.0
23	36.777945588888606	38.0	35.0	40.0	31.0	41.0
24	36.665712352183185	38.0	35.0	40.0	31.0	41.0
25	36.6395460190943	38.0	35.0	40.0	31.0	41.0
26	36.634244303146474	38.0	35.0	40.0	31.0	41.0
27	36.61789772042759	38.0	35.0	40.0	31.0	41.0
28	36.62451920989302	38.0	35.0	40.0	31.0	41.0
29	36.624740436179465	38.0	35.0	40.0	31.0	41.0
30	36.59041667681057	38.0	35.0	40.0	31.0	41.0
31	36.484083976492954	38.0	35.0	40.0	31.0	41.0
32	36.461611892254176	38.0	35.0	40.0	31.0	41.0
33	36.360056378477275	38.0	34.0	40.0	31.0	41.0
34	36.268100673122625	37.0	34.0	40.0	31.0	41.0
35	36.17994745595889	37.0	34.0	40.0	31.0	41.0
36	35.99779669189069	37.0	34.0	40.0	30.0	41.0
37	35.99457469596058	37.0	34.0	40.0	31.0	41.0
38	35.920041287134495	36.0	34.0	40.0	31.0	41.0
39	35.760904789864725	36.0	34.0	39.0	30.0	41.0
40	35.57475352738162	36.0	34.0	39.0	30.0	41.0
41	35.4321693808024	36.0	34.0	39.0	30.0	41.0
42	35.280019239869475	35.0	33.0	39.0	30.0	40.0
43	35.14857313080283	35.0	33.0	39.0	30.0	40.0
44	35.03053097978369	35.0	33.0	39.0	29.0	40.0
45	35.028913041497255	35.0	33.0	38.0	30.0	40.0
46	34.92227914851407	35.0	33.0	38.0	30.0	40.0
47	34.84557363146582	35.0	33.0	38.0	29.0	40.0
48	34.834632623324815	35.0	33.0	38.0	30.0	40.0
49	34.94746521379282	35.0	33.0	38.0	29.0	40.0
50	35.69421603037032	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	9.0
22	54.0
23	153.0
24	444.0
25	1125.0
26	2859.0
27	6797.0
28	15407.0
29	32728.0
30	62354.0
31	107802.0
32	170125.0
33	248565.0
34	346362.0
35	376966.0
36	458187.0
37	519578.0
38	534950.0
39	492688.0
40	244640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.083534223480775	16.193508360628716	35.72295741589051
2	29.040501165581617	20.263145615284348	28.63285821927623	22.063494999857806
3	30.24288771568368	16.206015963898583	26.237169007539627	27.31392731287811
4	20.97094342946333	16.953849152782443	29.58909624145141	32.48611117630281
5	23.97616431843088	18.939852233573554	27.83938470322881	29.244598744766755
6	27.24396204425593	19.15747900834862	24.35387737081896	29.244681576576493
7	32.50146267170689	19.495598455683737	21.410145295277456	26.592793577331914
8	27.496902780581024	16.661176758388173	28.05104758770301	27.7908728733278
9	28.281789399019324	17.17340866978464	23.734433487023153	30.81036844417288
10	29.310035874456798	19.680368612597558	24.877236355323063	26.13235915762258
11	35.46239615306987	16.35381552306769	23.775490454048086	24.408297869814348
12	32.26069821141273	16.62158315333521	22.387339765315396	28.73037886993666
13	29.429921113745472	17.746273465906565	25.96940137727211	26.854404043075853
14	28.59773753194892	17.98761774886886	21.417434494534067	31.997210224648153
15	26.519846363559306	18.846417952193345	23.340043630275247	31.293692053972105
16	27.831432849494327	20.23407165006763	26.27852969120025	25.65596580923779
17	28.889830103675052	19.76518838576541	18.675370265092162	32.66961124546737
18	29.031582940733564	19.911855910201485	21.168911454728136	29.887649694336815
19	30.751392195642108	16.685170372607853	25.415836392818257	27.14760103893178
20	30.493564382542697	17.63743246791579	24.10797733832128	27.76102581122023
21	32.23242495369011	19.93543536537249	20.66501794551158	27.167121735425816
22	30.80733127781596	19.746192290733024	23.20127273836717	26.245203693083845
23	31.233335275279096	21.611675088360833	20.632630707905495	26.522358928454576
24	32.961814259606484	22.552257898496244	19.8188081772667	24.66711966463057
25	30.966242767153574	21.413267873546634	21.72632066095427	25.89416869834552
26	32.215787175376846	20.96950477632815	21.98862724114011	24.82608080715489
27	30.711509945650494	20.522756463588554	22.925807950340772	25.83992564042018
28	31.968342699646453	20.699856705693605	22.54945596156879	24.782344633091157
29	33.436568862011775	19.848419045875772	23.264314899733176	23.450697192379277
30	30.324448432447976	21.966508885480536	21.656337273805427	26.052705408266064
31	28.758404103576435	19.95769152750446	26.54386847667546	24.74003589224365
32	28.494671235527612	19.290221787991637	24.855541631857424	27.359565344623327
33	27.778242275705274	19.364197881671252	25.308485305979993	27.54907453664348
34	27.158121402130753	17.349681683094637	29.656164929446955	25.836031985327658
35	31.185157685072816	18.284243111347923	23.60288250933348	26.927716694245785
36	28.22666543632589	20.11637720048179	22.359039918650847	29.297917444541472
37	26.902090711896737	20.486167369074074	25.89101832337683	26.720723595652363
38	30.178881916558232	20.32779275399604	22.338365591237483	27.154959738208245
39	28.450972200266477	20.472663218987826	25.484688692667284	25.59167588807841
40	28.61420215195655	19.11564863218799	23.43393138100797	28.83621783484748
41	27.027326116808396	20.320750123413546	23.948258369427187	28.703665390350874
42	27.219355594108812	20.88910771051067	25.91813454999885	25.97340214538167
43	25.652575603261095	23.14044239530233	24.07214664751672	27.134835353919858
44	24.097890837976966	23.53513034616643	24.624540670752996	27.74243814510361
45	27.299160051287263	19.274573486484105	27.001919390978962	26.42434707124967
46	26.349347680877212	20.146738128082085	23.84879679984748	29.655117391193226
47	27.79606080294425	20.230816720386024	24.385224987762463	27.587897488907267
48	25.056499673881454	20.68923283892271	24.942674094328453	29.311593392867387
49	27.742273457118067	20.91603370181261	22.936779092487335	28.40491374858199
50	23.86326194398682	25.544015471671084	24.538714991762767	26.054007592579325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1669.0
1	1620.5
2	1254.5
3	922.5
4	876.0
5	720.5
6	547.0
7	535.5
8	622.5
9	714.5
10	799.0
11	858.0
12	870.5
13	887.5
14	936.0
15	983.0
16	1053.5
17	1190.0
18	1323.0
19	1570.0
20	1926.0
21	2414.0
22	3050.5
23	3907.0
24	4820.0
25	5668.0
26	7000.5
27	8826.0
28	10746.5
29	12737.5
30	15320.0
31	19117.0
32	24114.0
33	27626.5
34	30870.0
35	35289.0
36	40503.0
37	45970.5
38	51176.5
39	60406.5
40	73528.0
41	90885.0
42	113724.0
43	130186.5
44	141624.0
45	154189.0
46	171655.0
47	186313.0
48	204717.0
49	238308.0
50	270650.5
51	284648.0
52	292266.0
53	312589.0
54	316772.0
55	313702.5
56	322880.0
57	339744.5
58	343637.0
59	315530.0
60	288012.5
61	273458.5
62	262753.5
63	250032.5
64	226029.0
65	204983.5
66	190884.0
67	179114.5
68	163074.5
69	144370.0
70	131078.5
71	119206.5
72	107733.5
73	95978.0
74	81678.0
75	69252.5
76	61131.5
77	49851.0
78	39362.0
79	31335.0
80	24469.5
81	18918.0
82	14303.5
83	10609.0
84	7930.5
85	5939.5
86	4312.0
87	3032.0
88	2204.0
89	1570.5
90	1045.5
91	624.0
92	426.5
93	362.5
94	260.5
95	148.5
96	108.5
97	98.0
98	51.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	34862.0
25	30542.0
26	28670.0
27	27235.0
28	25909.0
29	24231.0
30	34011.0
31	26433.0
32	29047.0
33	28329.0
34	27491.0
35	32231.0
36	26352.0
37	28018.0
38	30204.0
39	35752.0
40	34913.0
41	34386.0
42	38438.0
43	40090.0
44	41734.0
45	67557.0
46	298843.0
47	538985.0
48	707933.0
49	1070381.0
50	279220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.712143003623925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.166078822603716	11.840319995302053
2	12.77764288219167	5.293047332503824
3	6.870654802258193	4.2691795437872155
4	4.659253581233138	3.860125058585906
5	3.380317301053768	3.5006807668524855
6	2.5568344459762917	3.17745124089915
7	1.9583887496069117	2.839369948698262
8	1.5699739218022128	2.601401950426218
9	1.2919002352092346	2.408222017626215
>10	7.042279938125611	25.291406666137707
>50	0.40049499166760943	5.748920214473248
>100	0.2745754382520473	11.25688095648556
>500	0.027592209981676538	3.930363650336087
>1k	0.02117888549944854	8.379056730520018
>5k	0.0019389120527664153	2.8117494907183356
>10k+	8.948824858921918E-4	2.791824436647655
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	29472	0.8137396988290619	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	16294	0.44988716926984035	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	13887	0.3834284472597443	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	10381	0.28662567228367575	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	10244	0.282843019639146	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	10097	0.2787842609621688	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	8936	0.2467283505950223	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	8511	0.23499384421600658	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	8263	0.22814641461131033	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	7820	0.21591491737388926	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	7762	0.21431350238569416	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	7298	0.2015021824801335	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	7045	0.19451669985921352	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	6422	0.17731529403773874	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	6389	0.17640414413066222	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	5838	0.1611907017428089	No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG	5704	0.15749088090801336	No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT	5631	0.1554753068711471	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	5401	0.14912486812485626	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	4887	0.13493301805705843	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	4767	0.13161974566768928	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	4282	0.11822860309398898	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	4107	0.11339674752615897	No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC	4107	0.11339674752615897	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	3974	0.10972453729460817	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	3953	0.10914471462646856	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGC	3721	0.10273905467368823	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA	3710	0.10243533803799607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.12554541295384583	0.0	0.0	0.0	0.0
2	0.2808826668087692	0.0	0.0	0.0	0.0
3	0.38522313647065254	0.0	0.0	0.0	0.0
4	0.46482450562524624	0.0	0.0	0.0	0.0
5	0.5669009058210607	0.0	0.0	0.0	0.0
6	0.683583315133344	0.0	0.0	0.0	1.1044241297897149E-4
7	0.7799443204574966	0.0	0.0	0.0	1.1044241297897149E-4
8	0.8520908267360098	0.0	0.0	0.0	1.1044241297897149E-4
9	0.919819636495364	0.0	0.0	0.0	1.1044241297897149E-4
10	0.9833516345615174	0.0	0.0	0.0	1.1044241297897149E-4
11	1.0396220439743034	0.0	0.0	0.0	1.1044241297897149E-4
12	1.0886584753369668	0.0	0.0	0.0	1.1044241297897149E-4
13	1.1265678335919986	0.0	0.0	0.0	1.1044241297897149E-4
14	1.1653607311508625	0.0	0.0	0.0	1.1044241297897149E-4
15	1.2025246031182864	0.0	0.0	0.0	1.1044241297897149E-4
16	1.23380741659458	0.0	0.0	0.0	1.1044241297897149E-4
17	1.262135895523686	0.0	0.0	0.0	1.1044241297897149E-4
18	1.2931426029675324	0.0	0.0	0.0	1.1044241297897149E-4
19	1.318406304936472	0.0	0.0	0.0	1.1044241297897149E-4
20	1.3383135498759318	0.0	0.0	0.0	1.1044241297897149E-4
21	1.3589386704997546	0.0	0.0	0.0	1.1044241297897149E-4
22	1.37393122806165	0.0	0.0	0.0	1.1044241297897149E-4
23	1.390690864231209	0.0	0.0	0.0	1.1044241297897149E-4
24	1.404440944647091	0.0	0.0	0.0	1.1044241297897149E-4
25	1.419019343160315	0.0	0.0	0.0	1.1044241297897149E-4
26	1.4391750835289774	0.0	0.0	0.0	1.1044241297897149E-4
27	1.452428173086454	0.0	0.0	0.0	1.1044241297897149E-4
28	1.46789011090351	0.0	0.0	0.0	1.1044241297897149E-4
29	1.4813916958901894	0.0	0.0	0.0	1.1044241297897149E-4
30	1.4945067324314423	0.0	0.0	0.0	1.1044241297897149E-4
31	1.5077874325921634	0.0	0.0	0.0	1.1044241297897149E-4
32	1.5209300797366612	0.0	0.0	0.0	1.1044241297897149E-4
33	1.5334100724032849	0.0	0.0	0.0	1.1044241297897149E-4
34	1.5460281180861324	0.0	0.0	0.0	1.1044241297897149E-4
35	1.557983509291106	0.0	0.0	0.0	1.1044241297897149E-4
36	1.5685859809370872	0.0	0.0	0.0	1.1044241297897149E-4
37	1.5764826134650838	0.0	0.0	0.0	1.1044241297897149E-4
38	1.5829987158308432	0.0	0.0	0.0	1.1044241297897149E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCA	100	0.0	265.961	44
CTATCAT	640	0.0	241.02716	44
ATGTTCT	750	0.0	234.04568	44
ACTTCTA	490	0.0	173.68883	44
CCCATAC	290	0.0	128.39497	44
CGTGCGC	1215	0.0	118.204895	44
ACATTGG	400	0.0	99.735374	44
TCGTGCG	2400	0.0	88.20335	43
CGCTACC	400	0.0	86.437325	44
ACGACAC	135	4.402209E-5	78.80326	44
AATCCCG	1460	0.0	65.670586	43
TATGTTC	1740	0.0	65.31861	43
AACGACA	315	0.0	64.97957	43
CAAAACG	215	5.9097056E-6	61.851402	44
TCTTCTA	410	2.910383E-11	58.381683	44
TCGCCCC	415	3.274181E-11	57.67829	44
GTTCAGG	2720	0.0	56.835117	43
CCTATCA	565	0.0	56.248028	43
TAATCCC	5140	0.0	56.05144	42
TGTGTTA	1440	0.0	54.675194	42
>>END_MODULE