FastQCFastQC Report
Tue 24 May 2016
ERR841190.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841190.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6298719
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG796701.2648603628769595No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA367660.5837059884716241No Hit
AAGCAGTGGTATCAACGCAGAGTG265560.42160953679629143No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT258500.41040090850218913No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG254020.40328835117108736No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT230570.3660585588911015No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC210580.3343219470498684No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT196200.31149190811655514Illumina Single End Adapter 2 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC191240.3036172910714067No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG189200.3003785372867086No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC187560.297774833263716Illumina Single End Adapter 2 (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCG168670.26778460826717304Illumina Single End Adapter 2 (100% over 23bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA166980.2651015230239672No Hit
AAGCAGTGGTATCAACGCAGAGCG165320.2624660665128894No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA161430.25629020758030324No Hit
AAGCAGTGGTATCAACGCAGAGTGCA156460.2483997142911122No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG154710.2456213715836506Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTGCAGG152390.24193808296575858No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTC148030.2350160405631685No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAGCAGTGGT142400.22607771516716335No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG118360.1879112244886619No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG115310.18306897005565734No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA114130.1811955732586261No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT112380.17841723055116446No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG106610.1692566377385624Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGC105920.1681611768996204No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA105270.1671292210368489No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG104900.1665418000072713No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAGCAGTG102380.16254098650852658No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG98080.15571420157019228No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA90590.14382289478225652No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC90120.14307671131225252No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGAGC89650.14233052784224856No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG79720.12656541750790917No Hit
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTG79410.12607325394258737Illumina Single End Adapter 2 (100% over 30bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT73490.11667451746934575No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG72470.1150551405769967No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG71280.11316586753592278No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC70490.1119116442565544No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG69370.11013350492377894No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG69370.11013350492377894Illumina Single End Adapter 2 (100% over 34bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC66890.10619619640120474No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA66560.1056722803477977Illumina Single End Adapter 1 (100% over 32bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT66410.10543413668715813No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCT65990.10476733443736735No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT65850.1045450670207704No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT64100.10176672431330877No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT64090.10175084806926615Illumina Single End Adapter 2 (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCA63470.10076652093862261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA20050.0134.6123744
ATTAGTC4850.085.7922644
AATCGCA1750.083.53979544
ACTTCTA9150.079.8877844
TTAAGCC16700.078.1701443
TCGTGCG42900.077.7388143
CTATACG2600.073.5298544
CTATCAT8300.073.1650744
CGTGCGC17100.067.07986544
AATCCCG15600.067.0110943
ATGTTCT13050.062.90721544
CGCTACC6350.061.98440644
TGTGTTA37700.059.37465342
GTGTTAG25600.057.9656243
ATTAAGC21300.056.33875342
CAATGAA23950.055.7844643
AACGACA4300.055.73741543
ACGCTAC13750.053.03355843
TAATCCT32250.052.77605442
CCAGAAT16250.051.90342344
Produced by FastQC (version 0.11.5)