##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841190.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6298719
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.160790471840386	38.0	35.0	39.0	31.0	39.0
2	36.873045773275486	38.0	35.0	39.0	33.0	39.0
3	37.1727940236737	39.0	37.0	39.0	33.0	39.0
4	37.54840150830669	39.0	37.0	40.0	33.0	41.0
5	38.178930985808385	40.0	37.0	41.0	33.0	41.0
6	38.24613290416671	40.0	37.0	41.0	33.0	41.0
7	37.61903841082607	39.0	36.0	40.0	32.0	41.0
8	37.89236446331389	39.0	37.0	40.0	33.0	41.0
9	37.941140889123645	39.0	37.0	41.0	33.0	41.0
10	37.26351373985726	39.0	36.0	40.0	32.0	41.0
11	37.553936443267276	39.0	36.0	40.0	32.0	41.0
12	37.56103090168017	39.0	36.0	40.0	32.0	41.0
13	37.56142733149391	39.0	36.0	40.0	32.0	41.0
14	37.58900531997062	39.0	36.0	40.0	33.0	41.0
15	37.594158272499534	39.0	36.0	40.0	32.0	41.0
16	37.40668205709764	39.0	36.0	40.0	32.0	41.0
17	37.1275381549804	38.0	36.0	40.0	31.0	41.0
18	37.17743353847028	38.0	36.0	40.0	31.0	41.0
19	37.17629902207099	38.0	36.0	40.0	31.0	41.0
20	36.95823134831066	38.0	35.0	40.0	31.0	41.0
21	36.812246744139564	38.0	35.0	40.0	31.0	41.0
22	36.704436092481664	38.0	35.0	40.0	30.0	41.0
23	36.08644233851359	38.0	34.0	40.0	30.0	41.0
24	36.39445512651064	38.0	35.0	40.0	30.0	41.0
25	36.50702355138495	38.0	35.0	40.0	30.0	41.0
26	36.73925966008217	38.0	35.0	40.0	31.0	41.0
27	36.739829997701506	38.0	35.0	40.0	31.0	41.0
28	36.372975713046266	38.0	35.0	40.0	30.0	41.0
29	36.52107027661978	38.0	35.0	40.0	30.0	41.0
30	36.67578793087031	38.0	35.0	40.0	31.0	41.0
31	36.628805098633514	38.0	35.0	40.0	30.0	41.0
32	36.66012409263072	38.0	35.0	40.0	31.0	41.0
33	36.622666439301845	38.0	35.0	40.0	31.0	41.0
34	36.580257813846835	38.0	35.0	40.0	31.0	41.0
35	36.48462473549142	38.0	35.0	40.0	31.0	41.0
36	36.415939250118655	38.0	35.0	40.0	31.0	41.0
37	36.40529034676405	38.0	34.0	40.0	31.0	41.0
38	36.35933227975507	38.0	34.0	40.0	31.0	41.0
39	36.188107401955065	37.0	34.0	40.0	31.0	41.0
40	36.053151004416065	37.0	34.0	40.0	30.0	41.0
41	35.91612718475323	37.0	34.0	40.0	30.0	41.0
42	35.73516881520148	37.0	34.0	40.0	30.0	41.0
43	35.62360427477064	36.0	34.0	39.0	30.0	41.0
44	35.51862903893616	36.0	34.0	39.0	30.0	41.0
45	35.483972996779904	36.0	34.0	39.0	30.0	41.0
46	35.40368870293621	36.0	34.0	39.0	30.0	40.0
47	35.34916191048078	36.0	34.0	39.0	30.0	40.0
48	35.368654608699394	36.0	34.0	39.0	30.0	40.0
49	35.41769383105201	36.0	34.0	39.0	30.0	40.0
50	35.75761643898944	37.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	31.0
22	81.0
23	241.0
24	556.0
25	1535.0
26	3874.0
27	9947.0
28	24325.0
29	54072.0
30	106067.0
31	185174.0
32	288452.0
33	417116.0
34	573400.0
35	649035.0
36	783355.0
37	890869.0
38	943666.0
39	903162.0
40	463760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.29349015252149	20.75063199358473	26.955877853893785
2	24.684209598808902	35.9360530291953	22.542123247600028	16.83761412439577
3	39.04347852317272	14.549609214191012	20.434885252064745	25.972027010571512
4	17.393314418376182	15.626653610043567	27.014238926994523	39.96579304458574
5	19.923733698867977	30.972885121562022	22.233774835803917	26.86960634376609
6	37.13682099487213	17.26408179186911	20.710385714936642	24.88871149832212
7	27.744831925348628	20.098150115920397	31.538730335485678	20.618287623245298
8	37.837471396961824	15.292061766844972	24.66825397354605	22.20221286264715
9	38.562253689996325	17.75346701448342	20.766841638752258	22.917437656767987
10	26.97989861113029	16.12988291746306	35.58485463472811	21.305363836678538
11	29.489345373241765	28.96328602688896	20.85351005498102	20.693858544888254
12	27.759549203576157	16.437294630860656	33.275067517696854	22.528088647866337
13	24.16051581281845	16.862730342471224	22.040592698293096	36.936161146417234
14	23.187095661832192	31.108087215829123	19.009801834309485	26.6950152880292
15	20.859146121616156	31.651658059360958	21.40705117977163	26.082144639251254
16	23.837593008991192	20.779526757742328	20.897233231074445	34.48564700219203
17	37.6575776757147	18.67798833381835	16.9253144964873	26.739119493979647
18	23.51286031334308	18.741477433744862	20.496850232563162	37.2488120203489
19	23.531372013896796	29.93842081223182	22.82162452397067	23.708582649900716
20	38.04773637306253	16.06453629698356	21.60059847089543	24.287128859058484
21	27.28246807009489	30.295572798215005	19.3995477493122	23.022411382377907
22	35.79661197776881	18.652554590862046	22.6114071766021	22.93942625476704
23	25.701813336965817	20.20633084282693	29.596351258089143	24.495504562118107
24	38.45321564591149	19.058303759859744	19.322468584485193	23.166012009743568
25	26.2956494471595	20.176330296435005	21.44275973320122	32.08526052320427
26	26.507200981790618	29.33926034751892	20.090306274349786	24.063232396340677
27	33.61757089243004	20.141160936094774	21.964941272430398	24.276326899044793
28	27.664926071435165	21.967536184936705	27.35305224818366	23.014485495444468
29	32.2238079044315	20.38842365026738	24.0474247882185	23.34034365708262
30	27.179066581160292	21.38494018148885	22.967702405559386	28.468290831791464
31	27.388942199594567	20.008060151104672	28.293624677032735	24.30937297226802
32	26.951450715202153	19.65230850944398	26.111517770996834	27.284723004357037
33	25.157661002715543	19.946092533815936	28.614926500653677	26.281319962814848
34	25.375681933196038	20.397472282416274	28.4024179780113	25.824427806376388
35	29.317511593354645	19.71693830384344	24.102865697177076	26.862684405624844
36	26.60417655434267	23.467906976744185	23.23578547698149	26.692130991931656
37	24.866766816558993	21.8869973889538	28.45731502499687	24.788920769490336
38	30.087845786536622	21.54641847035246	22.13956506847257	26.22617067463835
39	27.174122629360863	22.488502945737043	25.221020999226173	25.116353425675918
40	26.985571368329346	21.765143545072235	23.72616243229934	27.52312265429908
41	26.34843963243203	24.20508522327372	22.652541292593764	26.793933851700487
42	24.973503515765664	23.646145124299473	26.844259531070474	24.53609182886439
43	24.552626167958643	24.50023622159972	25.35013601674984	25.597001593691793
44	23.167085168515037	23.54332595081604	25.910128264477716	27.37946061619121
45	26.02675901378352	21.827181619233517	27.119732212783386	25.026327154199578
46	24.91900133618474	22.436394208477704	24.749188588390428	27.895415866947133
47	27.83472710568243	20.828465683603003	25.187816575100307	26.14899063561426
48	25.6182957608523	23.47690362092981	23.713804081676574	27.190996536541316
49	26.929556934204523	22.380757057132577	23.56389760865467	27.125788400008226
50	22.744701014723898	25.008436770450498	26.89133901948547	25.35552319534013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4091.0
1	4049.5
2	3714.0
3	3355.0
4	3146.0
5	2627.5
6	1993.0
7	1792.0
8	1873.0
9	1935.0
10	2018.0
11	2100.5
12	2128.0
13	2172.5
14	2314.5
15	2575.5
16	2865.0
17	3315.0
18	3881.0
19	4810.0
20	5969.5
21	7471.0
22	9245.0
23	11824.5
24	14890.0
25	17805.5
26	21027.0
27	25938.0
28	32222.0
29	37741.5
30	42598.5
31	49351.5
32	58445.5
33	66064.0
34	74616.0
35	87358.0
36	100029.0
37	109456.0
38	118788.0
39	136926.5
40	160134.5
41	197908.0
42	257236.5
43	309149.0
44	329513.0
45	319802.0
46	319491.5
47	339033.5
48	438325.5
49	559570.0
50	711321.0
51	886108.5
52	941663.0
53	921518.0
54	818679.0
55	623491.0
56	469353.5
57	425137.0
58	418365.0
59	399001.5
60	377271.0
61	368672.0
62	360972.0
63	342303.0
64	304567.0
65	269380.0
66	238920.0
67	213057.0
68	191423.5
69	166112.5
70	145994.0
71	133499.0
72	125880.5
73	113903.0
74	93859.5
75	75765.5
76	64175.5
77	49916.0
78	38662.0
79	32058.0
80	25459.0
81	19102.0
82	14445.0
83	11663.0
84	9402.0
85	7250.0
86	5301.5
87	3542.5
88	2351.5
89	1654.5
90	1131.5
91	736.5
92	524.5
93	410.0
94	255.0
95	114.0
96	75.5
97	71.0
98	40.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	112034.0
25	77032.0
26	75264.0
27	132002.0
28	127095.0
29	72793.0
30	89701.0
31	81285.0
32	77723.0
33	59200.0
34	63950.0
35	63140.0
36	54647.0
37	53926.0
38	60882.0
39	65967.0
40	66357.0
41	72619.0
42	73475.0
43	73730.0
44	77713.0
45	106689.0
46	344881.0
47	685173.0
48	1149560.0
49	1300306.0
50	1081575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.24155888591242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.38280545115082	11.041346301271746
2	12.685015368429237	4.881589403606704
3	7.015237987658199	4.049523445146449
4	4.650396103882112	3.5792348190266146
5	3.3525700122860944	3.2254336655273503
6	2.6136543862306563	3.0174470868048275
7	1.9771360271903873	2.6630225504849943
8	1.5635406617759287	2.4067967771264005
9	1.253949556054541	2.171514980050847
>10	6.885546510790768	22.46673313195948
>50	0.32878464449507233	4.363580627812311
>100	0.2309529239275493	8.862905901134049
>500	0.029112376571275585	3.8453060027872312
>1k	0.02544054458447283	9.90273513521376
>5k	0.00332213296979369	4.187647320026642
>10k+	0.0025353120032636056	9.335182852020619
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTGCAG	79670	1.2648603628769595	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA	36766	0.5837059884716241	No Hit
AAGCAGTGGTATCAACGCAGAGTG	26556	0.42160953679629143	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT	25850	0.41040090850218913	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	25402	0.40328835117108736	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT	23057	0.3660585588911015	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	21058	0.3343219470498684	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	19620	0.31149190811655514	Illumina Single End Adapter 2 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	19124	0.3036172910714067	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	18920	0.3003785372867086	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC	18756	0.297774833263716	Illumina Single End Adapter 2 (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCG	16867	0.26778460826717304	Illumina Single End Adapter 2 (100% over 23bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	16698	0.2651015230239672	No Hit
AAGCAGTGGTATCAACGCAGAGCG	16532	0.2624660665128894	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	16143	0.25629020758030324	No Hit
AAGCAGTGGTATCAACGCAGAGTGCA	15646	0.2483997142911122	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG	15471	0.2456213715836506	Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTGCAGG	15239	0.24193808296575858	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTC	14803	0.2350160405631685	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAGCAGTGGT	14240	0.22607771516716335	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	11836	0.1879112244886619	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	11531	0.18306897005565734	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	11413	0.1811955732586261	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	11238	0.17841723055116446	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	10661	0.1692566377385624	Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGC	10592	0.1681611768996204	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	10527	0.1671292210368489	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	10490	0.1665418000072713	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAGCAGTG	10238	0.16254098650852658	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	9808	0.15571420157019228	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	9059	0.14382289478225652	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	9012	0.14307671131225252	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGAGC	8965	0.14233052784224856	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	7972	0.12656541750790917	No Hit
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTG	7941	0.12607325394258737	Illumina Single End Adapter 2 (100% over 30bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	7349	0.11667451746934575	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	7247	0.1150551405769967	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	7128	0.11316586753592278	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	7049	0.1119116442565544	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	6937	0.11013350492377894	No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	6937	0.11013350492377894	Illumina Single End Adapter 2 (100% over 34bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	6689	0.10619619640120474	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	6656	0.1056722803477977	Illumina Single End Adapter 1 (100% over 32bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	6641	0.10543413668715813	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCT	6599	0.10476733443736735	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	6585	0.1045450670207704	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	6410	0.10176672431330877	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT	6409	0.10175084806926615	Illumina Single End Adapter 2 (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCA	6347	0.10076652093862261	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.3665507224564233	0.0	0.0	0.0	0.0
2	1.104034010725038	0.0	0.0	0.0	0.0
3	1.2366165247251069	0.0	0.0	0.0	0.0
4	1.3649918340538767	0.0	0.0	0.0	0.0
5	1.9799740232894973	0.0	0.0	0.0	0.0
6	2.1404034693403533	0.0	0.0	0.0	0.0
7	2.2822259573732375	0.0	0.0	0.0	0.0
8	2.403123555757925	0.0	0.0	0.0	0.0
9	2.491363720146906	0.0	0.0	0.0	0.0
10	2.568871543563064	0.0	0.0	0.0	0.0
11	2.726236874513691	0.0	0.0	0.0	0.0
12	2.7925836983678747	0.0	0.0	0.0	0.0
13	2.8615501024890935	1.5876244042637876E-5	0.0	0.0	0.0
14	2.94632924567678	9.525746425582725E-4	0.0	0.0	0.0
15	3.1317002711186195	9.525746425582725E-4	0.0	0.0	0.0
16	3.196745242961307	9.525746425582725E-4	0.0	0.0	0.0
17	3.3219611797255917	9.525746425582725E-4	0.0	0.0	0.0
18	3.3826243082125114	9.525746425582725E-4	0.0	0.0	0.0
19	4.255738349337381	9.525746425582725E-4	0.0	0.0	0.0
20	4.3160204479672775	9.525746425582725E-4	0.0	0.0	0.0
21	4.661487518335077	9.525746425582725E-4	0.0	0.0	0.0
22	4.697256696163141	9.525746425582725E-4	0.0	0.0	0.0
23	4.73547081557377	9.525746425582725E-4	0.0	0.0	0.0
24	4.766508872677127	9.525746425582725E-4	0.0	0.0	0.0
25	4.818424190696553	9.525746425582725E-4	0.0	0.0	0.0
26	5.653054216262069	9.525746425582725E-4	0.0	0.0	0.0
27	5.68947432009588	9.525746425582725E-4	0.0	0.0	0.0
28	5.724529066942024	9.525746425582725E-4	0.0	0.0	0.0
29	5.754725683111121	9.525746425582725E-4	0.0	0.0	0.0
30	5.784557145667238	9.525746425582725E-4	0.0	0.0	0.0
31	5.8008779245430695	9.525746425582725E-4	0.0	0.0	0.0
32	5.8219933291197785	9.525746425582725E-4	0.0	0.0	0.0
33	5.836393082466451	9.525746425582725E-4	0.0	0.0	0.0
34	5.850872217033336	9.525746425582725E-4	0.0	0.0	0.0
35	5.8693362888549245	9.525746425582725E-4	0.0	0.0	0.0
36	5.885593562754585	9.525746425582725E-4	0.0	0.0	0.0
37	5.905756392688736	9.525746425582725E-4	0.0	0.0	0.0
38	5.9143613169598455	9.525746425582725E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	2005	0.0	134.61237	44
ATTAGTC	485	0.0	85.79226	44
AATCGCA	175	0.0	83.539795	44
ACTTCTA	915	0.0	79.88778	44
TTAAGCC	1670	0.0	78.17014	43
TCGTGCG	4290	0.0	77.73881	43
CTATACG	260	0.0	73.52985	44
CTATCAT	830	0.0	73.16507	44
CGTGCGC	1710	0.0	67.079865	44
AATCCCG	1560	0.0	67.01109	43
ATGTTCT	1305	0.0	62.907215	44
CGCTACC	635	0.0	61.984406	44
TGTGTTA	3770	0.0	59.374653	42
GTGTTAG	2560	0.0	57.96562	43
ATTAAGC	2130	0.0	56.338753	42
CAATGAA	2395	0.0	55.78446	43
AACGACA	430	0.0	55.737415	43
ACGCTAC	1375	0.0	53.033558	43
TAATCCT	3225	0.0	52.776054	42
CCAGAAT	1625	0.0	51.903423	44
>>END_MODULE