FastQCFastQC Report
Tue 24 May 2016
ERR841189.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841189.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4106236
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC194220.4729879139922791No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG131140.3193679077383765No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA113180.2756295546578424No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG112230.2733160003467896Illumina Single End Adapter 1 (100% over 32bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG110110.26815312125265084No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG102080.24859749902343656No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG97880.23836915364825595No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG94190.2293828216400616No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA92170.22446347457866522No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT91930.2238789977000835No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT91790.22353805285424416No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC89520.21800987571099179No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT84430.20561409524440388No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG62280.15167174999196345No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA59630.14521815112428998Illumina Single End Adapter 1 (100% over 32bp)
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG55830.135963933880079No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC55120.13423485644760796No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT53690.13075234837939173No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC51650.12578429491144688No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC49390.12028047097146877No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG48310.1176503250178509No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG46880.11416781694963465No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA46320.11280403756627724Illumina Single End Adapter 1 (100% over 32bp)
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC46140.11236567990734092No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG45910.11180555623203342No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG45340.11041742364540177No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG43750.10654526432479769No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT43720.10647220471497497No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA42570.10367158633843743No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA41810.10182074288959524Illumina Single End Adapter 1 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCA1050.0239.7765244
CCCATAC3250.0147.8901444
CTATCAT7600.0120.4618844
ATGTTCT7500.0109.86124444
ACATTGG3850.089.1730944
GACCGTA1103.358924E-583.2282244
TCGTGCG18200.080.9707143
ACTTCTA6600.079.76037644
TCACGTC1805.6304998E-876.29253444
ATCCTTG20150.072.61613543
GATACCC1754.2496795E-665.3935944
TAATTCC3106.0936145E-1059.0651844
CTAAAAC9300.059.00061443
CATGACC5550.057.7348944
CCCGAGG11900.057.70023344
AATCCCG11200.057.39014443
CCAGAAT6550.055.90903544
TGTGTTA16750.055.81634542
CACATGC14800.055.6729344
TACGCTG1652.5002196E-455.4854844
Produced by FastQC (version 0.11.5)