##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841189.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4106236
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02886828716128	38.0	35.0	39.0	31.0	39.0
2	36.79374687670168	38.0	35.0	39.0	32.0	39.0
3	37.12353966016566	39.0	36.0	39.0	33.0	40.0
4	37.74784571563836	39.0	37.0	40.0	33.0	41.0
5	38.049984219124276	39.0	37.0	41.0	33.0	41.0
6	38.08173689968136	40.0	37.0	41.0	33.0	41.0
7	38.038930787222164	40.0	37.0	41.0	33.0	41.0
8	37.921176230494304	39.0	37.0	41.0	33.0	41.0
9	37.859569688639425	39.0	37.0	41.0	33.0	41.0
10	37.73515574847622	39.0	36.0	40.0	33.0	41.0
11	37.64294721491897	39.0	36.0	40.0	33.0	41.0
12	37.49302646024242	39.0	36.0	40.0	32.0	41.0
13	37.37573948501742	39.0	36.0	40.0	32.0	41.0
14	37.324000617597235	39.0	36.0	40.0	32.0	41.0
15	37.26719944981243	39.0	36.0	40.0	32.0	41.0
16	37.24144593734992	39.0	36.0	40.0	32.0	41.0
17	37.167341331574704	38.0	35.0	40.0	31.0	41.0
18	37.06233616382497	38.0	35.0	40.0	31.0	41.0
19	36.959666224737205	38.0	35.0	40.0	31.0	41.0
20	36.85395530115658	38.0	35.0	40.0	31.0	41.0
21	36.676151833455265	38.0	35.0	40.0	31.0	41.0
22	36.71165271552828	38.0	35.0	40.0	31.0	41.0
23	36.732809804404816	38.0	35.0	40.0	31.0	41.0
24	36.65679274157647	38.0	35.0	40.0	31.0	41.0
25	36.61074964340222	38.0	35.0	40.0	31.0	41.0
26	36.590373079635015	38.0	35.0	40.0	31.0	41.0
27	36.60087677930835	38.0	35.0	40.0	31.0	41.0
28	36.596165191219605	38.0	35.0	40.0	31.0	41.0
29	36.59456335977666	38.0	35.0	40.0	31.0	41.0
30	36.581724820604805	38.0	35.0	40.0	31.0	41.0
31	36.47425127117252	38.0	35.0	40.0	31.0	41.0
32	36.410976402157544	38.0	34.0	40.0	31.0	41.0
33	36.27140602317417	38.0	34.0	40.0	31.0	41.0
34	36.23180311113858	37.0	34.0	40.0	31.0	41.0
35	36.140589389714656	37.0	34.0	40.0	31.0	41.0
36	36.00847394226707	37.0	34.0	40.0	30.0	41.0
37	35.94838568610641	37.0	34.0	40.0	30.0	41.0
38	35.87603647412366	37.0	34.0	40.0	30.0	41.0
39	35.72023868309063	36.0	34.0	39.0	30.0	41.0
40	35.56973571082518	36.0	34.0	39.0	30.0	41.0
41	35.408432293640544	36.0	34.0	39.0	30.0	41.0
42	35.27504745053087	35.0	33.0	39.0	30.0	41.0
43	35.14185323408827	35.0	33.0	39.0	29.0	40.0
44	35.06584747815617	35.0	33.0	39.0	29.0	40.0
45	35.045828866137455	35.0	33.0	39.0	30.0	40.0
46	34.94569261332852	35.0	33.0	38.0	30.0	40.0
47	34.91151532011177	35.0	33.0	38.0	29.0	40.0
48	34.90334456901058	35.0	33.0	38.0	30.0	40.0
49	35.03966941298095	35.0	33.0	39.0	29.0	40.0
50	35.87584902440412	37.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	23.0
22	61.0
23	207.0
24	526.0
25	1390.0
26	3399.0
27	7989.0
28	18047.0
29	38580.0
30	73843.0
31	126915.0
32	198282.0
33	287102.0
34	394946.0
35	431600.0
36	522164.0
37	588791.0
38	598346.0
39	545795.0
40	268226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.33868194619111	16.517462707939824	36.14385534586906
2	27.85911477080226	19.63615827244221	30.001490416040383	22.503236540715147
3	28.749126937662616	15.945746907873781	26.841418759175067	28.463707395288534
4	21.38459650151623	17.382732020273554	32.0985934563917	29.13407802181852
5	23.46246051128089	18.993428531628478	29.353427323709596	28.19068363338103
6	26.16963564685517	19.093276665052862	26.08086822092057	28.656219467171397
7	30.821852421536416	19.05382447574859	22.852948539733227	27.271374562981766
8	28.239877103995	16.960861479953905	26.658891500634645	28.140369915416453
9	27.603430489626025	18.60190208258853	24.55784324135291	29.236824186432536
10	28.83811354242669	18.863138894111298	25.55566703910832	26.74308052435369
11	32.53181258943714	16.56970032896307	24.03247158711774	26.866015494482053
12	29.874196222525935	17.281593167075638	23.99991135434008	28.84429925605835
13	27.91481054669045	17.896828141392753	24.782379775541397	29.405981536375403
14	28.65285872511955	18.639138130394844	22.296502198120127	30.41150094636548
15	26.315998398533353	19.733473672726067	24.18280391092962	29.76772401781096
16	28.236029297877668	20.768947522743456	24.749137653072058	26.245885526306818
17	28.311037163962325	19.769857358417784	20.552496252042015	31.36660922557788
18	29.07765164983211	19.131365074973772	21.45000920551084	30.34097406968328
19	28.79530061107058	17.266250649012868	25.05382057923607	28.884628160680485
20	29.489927028061707	17.670416410552146	23.924927841458697	28.914728719927446
21	31.086279502688107	20.18464111658463	21.674326560869858	27.05475281985741
22	29.623114696768525	19.455725389383367	24.156770336629457	26.76438957721865
23	29.7632430284085	21.051907391586845	22.588984169443744	26.595865410560915
24	32.8108272393501	20.8059643917203	21.89211725775138	24.491091111178218
25	30.415497575921947	20.770477416867415	23.155311886895866	25.658713120314776
26	30.38207082097169	20.91572152530607	22.756433635173202	25.945774018549034
27	30.2758304773251	20.978982664807667	23.64409554958071	25.10109130828652
28	31.591583608400654	20.69573977114029	22.191305611121102	25.521371009337955
29	31.59029516689999	19.86340156138644	23.96338803980834	24.58291523190523
30	30.47612659467732	20.864015508285753	23.80520327359595	24.85465462344097
31	28.65362673144471	20.4446601183315	26.399414990329596	24.502298159894192
32	29.303729257619132	19.225600384120582	25.14181408250764	26.32885627575265
33	27.58136036107372	19.82563940137778	26.342544936257816	26.250455301290682
34	27.35472223347181	18.643704718929648	29.178381039873987	24.823192007724558
35	28.964496810475858	19.735185650250287	24.13500358399206	27.165313955281796
36	28.445856299632982	21.277677559273933	22.8775493714799	27.39891676961318
37	26.27932216314244	22.015661194743636	26.35953174088021	25.345484901233718
38	29.728691246121546	21.382549948287267	22.19416800202412	26.69459080356707
39	28.322967355587082	21.343299411035893	24.565487466556533	25.768245766820492
40	27.93516903180022	20.72425059877039	23.075718012696434	28.264862356732955
41	26.171655863491793	21.639131721938142	24.14374231807296	28.045470096497098
42	27.503899414497333	21.780442478934123	25.315620023672047	25.400038082896494
43	25.104921074869623	23.68303871065792	24.18350203692063	27.028538177551827
44	24.389236263512235	22.98809532375395	24.79514291127916	27.82752550145466
45	26.86414410555279	19.810406325184278	26.266990049054982	27.05845952020795
46	25.97971016297883	21.044665043313632	23.660235531502806	29.31538926220473
47	27.129246960284352	20.884454310963356	24.83846023546521	27.14783849328708
48	24.667199125066197	22.07564761834118	25.162281934997527	28.0948713215951
49	27.438632516901507	21.688098725784798	22.636088589704247	28.237180167609445
50	24.133625348427103	25.833096308094888	24.711018829284942	25.322259514193075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2510.0
1	2476.0
2	2134.5
3	1783.5
4	1696.0
5	1427.5
6	1065.0
7	1011.5
8	1170.0
9	1309.5
10	1405.5
11	1452.5
12	1457.0
13	1442.5
14	1478.0
15	1573.5
16	1656.5
17	1777.0
18	2029.5
19	2535.0
20	3106.5
21	3943.0
22	4949.0
23	6202.0
24	7672.0
25	8953.0
26	10530.5
27	12514.5
28	14981.0
29	18065.0
30	21686.5
31	26461.5
32	32736.5
33	37861.5
34	42926.5
35	49470.5
36	56245.0
37	62672.0
38	69989.0
39	83049.0
40	100119.5
41	122151.0
42	152299.0
43	174501.5
44	185442.0
45	192288.0
46	205055.5
47	218612.0
48	239602.5
49	276632.5
50	309187.5
51	323680.5
52	349731.0
53	386045.5
54	382517.5
55	365690.0
56	355835.5
57	353996.5
58	348442.0
59	322300.0
60	300869.5
61	288431.0
62	275767.5
63	261702.0
64	244719.0
65	230250.0
66	213358.0
67	196579.5
68	175819.0
69	153237.0
70	137318.5
71	122799.0
72	109749.0
73	98257.5
74	86272.0
75	74884.5
76	65889.0
77	53284.0
78	42492.5
79	34914.0
80	27087.0
81	20311.5
82	15327.5
83	11390.5
84	8335.5
85	6156.5
86	4631.0
87	3370.5
88	2345.5
89	1608.0
90	1070.0
91	644.5
92	431.0
93	343.5
94	230.0
95	121.0
96	97.0
97	95.0
98	50.5
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	39329.0
25	36293.0
26	33485.0
27	32342.0
28	30335.0
29	28411.0
30	40122.0
31	33789.0
32	43430.0
33	35476.0
34	38093.0
35	39274.0
36	35394.0
37	35935.0
38	40304.0
39	47016.0
40	44772.0
41	43075.0
42	47295.0
43	46570.0
44	48572.0
45	75464.0
46	306912.0
47	585744.0
48	764357.0
49	1202867.0
50	351580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.557498025154356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.38333923549227	11.796578830103947
2	12.807253290179727	5.265701684410427
3	6.828874311337361	4.211537105080353
4	4.565577702579102	3.754274184178345
5	3.211915498058187	3.301447325414692
6	2.427332698868413	2.9939932298027996
7	1.988070131520293	2.860882346181775
8	1.5020264297884522	2.47023242912846
9	1.2420538919556117	2.298016838791149
>10	7.322185385033807	26.288651757538915
>50	0.3997861097218061	5.665003383107673
>100	0.2647039934184618	10.636833567452959
>500	0.03257867867789978	4.6771346186431675
>1k	0.021806696211779	9.072466961501382
>5k	0.0017077533177899218	2.646834964127626
>10k+	7.881938389799638E-4	2.0604107745363414
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	19422	0.4729879139922791	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	13114	0.3193679077383765	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	11318	0.2756295546578424	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	11223	0.2733160003467896	Illumina Single End Adapter 1 (100% over 32bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	11011	0.26815312125265084	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	10208	0.24859749902343656	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	9788	0.23836915364825595	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	9419	0.2293828216400616	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	9217	0.22446347457866522	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	9193	0.2238789977000835	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	9179	0.22353805285424416	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	8952	0.21800987571099179	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	8443	0.20561409524440388	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	6228	0.15167174999196345	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	5963	0.14521815112428998	Illumina Single End Adapter 1 (100% over 32bp)
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	5583	0.135963933880079	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	5512	0.13423485644760796	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT	5369	0.13075234837939173	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	5165	0.12578429491144688	No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC	4939	0.12028047097146877	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	4831	0.1176503250178509	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	4688	0.11416781694963465	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	4632	0.11280403756627724	Illumina Single End Adapter 1 (100% over 32bp)
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	4614	0.11236567990734092	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	4591	0.11180555623203342	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	4534	0.11041742364540177	No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG	4375	0.10654526432479769	No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT	4372	0.10647220471497497	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA	4257	0.10367158633843743	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	4181	0.10182074288959524	Illumina Single End Adapter 1 (100% over 32bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.3321776926606264	0.0	0.0	0.0	0.0
2	0.6208362110701869	0.0	0.0	0.0	0.0
3	0.7817621783063613	0.0	0.0	0.0	0.0
4	0.9014094659926999	0.0	0.0	0.0	0.0
5	1.0476748048577822	0.0	0.0	0.0	0.0
6	1.1758457136901046	0.0	0.0	0.0	0.0
7	1.276887154074924	0.0	0.0	0.0	0.0
8	1.3521385521923242	0.0	0.0	0.0	0.0
9	1.412826734751729	0.0	0.0	0.0	0.0
10	1.466647313987798	0.0	0.0	0.0	0.0
11	1.5107996715239942	0.0	0.0	0.0	0.0
12	1.5467206463534975	4.870640654847895E-5	0.0	0.0	0.0
13	1.5742154128501138	4.870640654847895E-5	0.0	0.0	0.0
14	1.6029765459169907	4.870640654847895E-5	0.0	0.0	0.0
15	1.635561131897923	4.870640654847895E-5	0.0	0.0	0.0
16	1.6556281713958965	4.870640654847895E-5	0.0	0.0	0.0
17	1.6792264253686344	4.870640654847895E-5	0.0	0.0	0.0
18	1.6989525200207685	4.870640654847895E-5	0.0	0.0	0.0
19	1.7330957110112521	4.870640654847895E-5	0.0	0.0	0.0
20	1.747415394536505	4.870640654847895E-5	0.0	0.0	0.0
21	1.7717685978107445	4.870640654847895E-5	0.0	0.0	0.0
22	1.783506841788928	4.870640654847895E-5	0.0	0.0	0.0
23	1.7958539158489673	4.870640654847895E-5	0.0	0.0	0.0
24	1.8090289988203307	4.870640654847895E-5	0.0	0.0	0.0
25	1.8213273664738219	4.870640654847895E-5	0.0	0.0	0.0
26	1.8528891179172362	4.870640654847895E-5	0.0	0.0	0.0
27	1.8649196003347104	4.870640654847895E-5	0.0	0.0	0.0
28	1.8779729172897028	4.870640654847895E-5	0.0	0.0	0.0
29	1.8906365829923073	4.870640654847895E-5	0.0	0.0	0.0
30	1.9022287077508453	9.74128130969579E-5	0.0	0.0	0.0
31	1.9143566029814165	9.74128130969579E-5	0.0	0.0	0.0
32	1.9263627321956167	9.74128130969579E-5	0.0	0.0	0.0
33	1.9379548569541547	1.2176601637119737E-4	0.0	0.0	0.0
34	1.9475743722474792	1.2176601637119737E-4	0.0	0.0	0.0
35	1.960164978340261	1.2176601637119737E-4	0.0	0.0	0.0
36	1.9708316813743778	1.2176601637119737E-4	0.0	0.0	0.0
37	1.9797206005694754	1.2176601637119737E-4	0.0	0.0	0.0
38	1.9859063142011322	1.2176601637119737E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCA	105	0.0	239.77652	44
CCCATAC	325	0.0	147.89014	44
CTATCAT	760	0.0	120.46188	44
ATGTTCT	750	0.0	109.861244	44
ACATTGG	385	0.0	89.17309	44
GACCGTA	110	3.358924E-5	83.22822	44
TCGTGCG	1820	0.0	80.97071	43
ACTTCTA	660	0.0	79.760376	44
TCACGTC	180	5.6304998E-8	76.292534	44
ATCCTTG	2015	0.0	72.616135	43
GATACCC	175	4.2496795E-6	65.39359	44
TAATTCC	310	6.0936145E-10	59.06518	44
CTAAAAC	930	0.0	59.000614	43
CATGACC	555	0.0	57.73489	44
CCCGAGG	1190	0.0	57.700233	44
AATCCCG	1120	0.0	57.390144	43
CCAGAAT	655	0.0	55.909035	44
TGTGTTA	1675	0.0	55.816345	42
CACATGC	1480	0.0	55.67293	44
TACGCTG	165	2.5002196E-4	55.48548	44
>>END_MODULE