FastQCFastQC Report
Tue 24 May 2016
ERR841188.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841188.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5039532
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC431320.8558731247266612No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC305110.6054332029243986No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA294160.5837049948288849No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG289760.5749740253658475No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC280150.555904794334077No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT236060.46841651169195875No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA189950.37691992034180954No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA178140.3534852045785204No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA170910.3391386343017566No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT167740.33284836766588644No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA160370.3182239938152987No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA143620.28498678051850845No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC140000.27780357382391857No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG127060.2521265863576221No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA125140.24831670877375123No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG121870.24182801101372112No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC120560.23922856328722586No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG113130.2244851307621422No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC109910.21809564856419209No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG107800.2139087518444173No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT105710.2097615413494745No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC102850.20408641119850018No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG102640.2036697058377643No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC98890.19622853868176648No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC94400.1873189812069851No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA92320.18319161382445828No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT91500.18156447860634678No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG90280.1791436188915955Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG88250.17511546707114867No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC84770.1682100639503827No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG84140.16695994786817506No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT78490.15574858935313834No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG77520.1538238074487869No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA77510.15380396433637092No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG77450.15368490566187495No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT76410.15162122197061156No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT75530.14987502807800407No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT74960.1487439706702924No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG73550.14594609181963722No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA73460.14576750380789327No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG71990.14285056628274212Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG62110.12324557121573987No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG60110.11927694873254102No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC59520.11810620509999739No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC58610.11630048187014191No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC57170.11344307368223874No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG55520.11016896013359971No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG55260.10965303921078387No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC54400.10794653154300837No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA54250.10764888485676845Illumina Single End Adapter 1 (100% over 32bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA52910.10498990779302522No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG51120.10143799067056226No Hit
TCAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT50460.10012834525110666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA42250.0193.4531744
AATCGCA7650.0167.3629244
ACATTGG15000.0167.2614744
CTATACG1300.0159.1703244
GAACACA35350.0141.9475144
ACTTCTA22600.0122.4590244
CATGACC10000.0115.1000344
CTATCAT23750.0112.7177244
CACATGC65600.0107.6401444
CGTGCGC15500.0103.4607244
CAGGCAC27750.094.6059644
ACCACTC4000.090.5281244
TTAAGCC34800.089.03329543
AATGAAG14300.085.91579444
TCGTGCG43600.084.5885743
ATTAGTC3000.081.90639544
CCAGAAT11600.081.38611644
CCCATAC10750.075.79098544
CAATGAA26950.074.61191643
ACACATG38900.068.8388843
Produced by FastQC (version 0.11.5)