FastQCFastQC Report
Tue 24 May 2016
ERR841187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808356
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC179430.6389147244864968No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG154010.5483991345826525No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT100930.35939175802497975No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT91340.3252436656891078No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC84420.300602915015048No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC82610.2941578631768907No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG73410.261398483668025No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA69520.24754696341916768No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG68350.24338082493814886No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC68080.24241940836560605No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA68030.24224136825957962No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG54170.19288865086904938No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC53560.19071656157552674No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG52540.1870845434125873No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA44290.15770792591822405No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA41650.1483074083200278No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA39400.14029560354883783No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT39190.13954783510352675No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA38190.13598703298299789No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC38040.13545291266491855No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC37630.1339929837955017No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC35700.12712063570288096No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC34630.12331057743391508No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA33680.11992781541941265No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA33100.1178625501895059No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG32610.11611775715044674No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA32570.11597532506562559No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC31510.11220087481786498No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT31420.11188040262701737No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG30050.1070021037218928No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG30000.10682406361586635No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG29700.1057558229797077No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG29370.10458075827993317No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC28320.10084191605337785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCA2250.0160.3847844
ATTAGTC2950.0126.0342844
TAAGCCA7300.0106.3572844
CGTGCGC11900.0102.0017844
CTATCAT6300.081.5810144
TCGTGCG27250.073.8438243
GAACACA13650.068.8965644
TTAAGCC4950.068.85271543
ATGTTCT7150.067.2943144
TAATCGC2500.063.49540743
CTATACG701.3899774E-462.4875844
ATCCTTG11150.061.5525343
AATCCCG11050.059.15196643
CAATGAA16450.058.7543
AGGTCCC9100.055.9227543
ACTTCTA7150.055.05898744
ATTAAGC6150.052.5013742
TCGCAAA1900.051.60230343
CACATGC14100.049.6355344
AACGACA2950.049.0617643
Produced by FastQC (version 0.11.5)