##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841187.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2808356
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.76004110589968	37.0	35.0	39.0	30.0	39.0
2	36.62614177119995	38.0	35.0	39.0	32.0	39.0
3	36.96572265054715	38.0	35.0	39.0	33.0	39.0
4	37.45878656409658	39.0	36.0	40.0	33.0	41.0
5	37.9168331935125	39.0	37.0	40.0	33.0	41.0
6	37.94997108628678	39.0	37.0	40.0	33.0	41.0
7	37.9040143058786	39.0	37.0	40.0	33.0	41.0
8	37.80557415085552	39.0	37.0	40.0	33.0	41.0
9	37.71504360558277	39.0	36.0	40.0	33.0	41.0
10	37.58044991446953	39.0	36.0	40.0	32.0	41.0
11	37.43393216529528	39.0	36.0	40.0	32.0	41.0
12	37.35024761817946	39.0	36.0	40.0	32.0	41.0
13	37.18031474642104	38.0	36.0	40.0	31.0	41.0
14	37.112963598632085	38.0	35.0	40.0	31.0	41.0
15	37.09671423423526	38.0	35.0	40.0	31.0	41.0
16	37.035997216877064	38.0	35.0	40.0	31.0	41.0
17	37.00279166886249	38.0	35.0	40.0	31.0	41.0
18	36.89242709969819	38.0	35.0	40.0	31.0	41.0
19	36.78031560101355	38.0	35.0	40.0	31.0	41.0
20	36.67256394844528	38.0	35.0	40.0	31.0	41.0
21	36.506049446722564	38.0	35.0	40.0	30.0	41.0
22	36.515925687484064	38.0	35.0	40.0	31.0	41.0
23	36.5067032099919	38.0	35.0	40.0	31.0	41.0
24	36.436088230979266	38.0	35.0	40.0	30.0	41.0
25	36.39750029391177	38.0	35.0	40.0	30.0	41.0
26	36.34811547068607	38.0	34.0	40.0	30.0	41.0
27	36.33688422072586	38.0	34.0	40.0	31.0	41.0
28	36.344935584520805	38.0	34.0	40.0	31.0	41.0
29	36.32349763748499	38.0	34.0	40.0	31.0	41.0
30	36.28756378706839	37.0	34.0	40.0	31.0	41.0
31	36.224763643422385	37.0	34.0	40.0	31.0	41.0
32	36.1736271606733	37.0	34.0	40.0	31.0	41.0
33	36.092816577978176	37.0	34.0	40.0	31.0	41.0
34	36.016078566425854	37.0	34.0	40.0	31.0	41.0
35	35.9003355162572	37.0	34.0	40.0	31.0	41.0
36	35.759742178248715	36.0	34.0	39.0	30.0	41.0
37	35.69849533473926	36.0	34.0	39.0	30.0	41.0
38	35.62569105104672	36.0	34.0	39.0	30.0	41.0
39	35.43135399614317	35.0	34.0	39.0	30.0	41.0
40	35.29422792102055	35.0	33.0	39.0	30.0	40.0
41	35.14850434466744	35.0	33.0	39.0	30.0	40.0
42	35.025491390079914	35.0	33.0	39.0	30.0	40.0
43	34.923198363047874	35.0	33.0	38.0	29.0	40.0
44	34.82478250169966	35.0	33.0	38.0	29.0	40.0
45	34.75193720443148	35.0	33.0	38.0	29.0	40.0
46	34.66558826902509	35.0	33.0	38.0	29.0	40.0
47	34.64988649488231	35.0	33.0	38.0	29.0	40.0
48	34.66801382164925	35.0	33.0	38.0	29.0	40.0
49	34.77968835207166	35.0	33.0	38.0	29.0	40.0
50	35.382364150100855	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	13.0
22	43.0
23	106.0
24	342.0
25	856.0
26	2402.0
27	5971.0
28	13708.0
29	29486.0
30	56073.0
31	95303.0
32	146708.0
33	210760.0
34	281816.0
35	321577.0
36	382445.0
37	419871.0
38	388540.0
39	315716.0
40	136619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.558026119195716	23.462231996228397	31.979741884575887
2	28.571947431166134	21.264789791607615	27.62025184841238	22.54301092881387
3	27.940830863323598	18.024032565671874	25.158348870299918	28.87678770070461
4	20.79593897639758	18.966363238848636	30.688381387544883	29.5493163972089
5	23.347218087735317	19.093412658509106	28.92211671169894	28.637252542056636
6	27.448656794224096	18.75061423836579	25.339842954383272	28.460886013026837
7	29.625375130503397	20.761684059998093	21.8901378600149	27.72280294948361
8	28.387319841216712	16.527498650455996	26.64583834812965	28.439343160197637
9	28.004604829302266	18.941081543792883	23.798051244215475	29.256262382689375
10	28.463699046702057	19.324117027898172	26.510848339740406	25.70133558565937
11	32.73245984483449	17.18731528339	25.033898836187436	25.04632603558808
12	30.897400472019932	17.32365839658505	23.275361100943044	28.50358003045198
13	27.29896067307706	18.774614044658154	26.382801895486185	27.5436233867786
14	27.011176645695915	19.655805745425436	21.63254943461584	31.70046817426281
15	24.533677354295538	20.74726281140995	23.21664347397552	31.502416360318993
16	26.49977424514556	22.402359245052978	25.740361976900367	25.3575045329011
17	26.56657489292668	21.379625659994673	18.650306442630495	33.40349300444815
18	25.667187493323496	20.84603946223342	22.29727997447617	31.18949306996691
19	26.98724805544596	18.1332779747297	27.119603070266017	27.75987089955832
20	26.881421016423847	18.168458699680524	25.429681991884216	29.520438292011413
21	28.635757005166013	20.652509866982676	22.207512153017635	28.50422097483368
22	28.37546237015535	19.93322783863584	24.82669576079386	26.864614030414945
23	28.487093516633934	21.11641116724518	23.831950080402912	26.56454523571798
24	31.33391920397556	21.63148119397968	20.611026522278515	26.42357307976624
25	27.594917321811273	23.001408978851657	23.810843780233814	25.59282991910326
26	30.051062464988433	21.51117902992201	22.225124735508476	26.21263376958108
27	27.541442266244836	22.642103190895327	23.661153716392995	26.155300826466842
28	27.97541811307417	20.965034970195	24.792184031366	26.267362885364836
29	30.570907049625827	20.284022260802324	24.916521991278586	24.228548698293263
30	27.5619298106346	22.620762943513903	23.294617996528128	26.52268924932337
31	27.05352833522036	20.69786465229506	27.786447101974165	24.462159910510415
32	26.904975471046853	20.376936428701633	26.10675222655764	26.611335873693875
33	25.53598889519301	18.61748070439977	27.45721877713534	28.38931162327188
34	25.03902395610434	19.587239920907322	30.121463756283106	25.25227236670523
35	28.29602383187818	19.90846860515301	24.51426626988494	27.28124129308387
36	25.970633027877117	20.05071502912352	23.820385125377292	30.158266817622074
37	24.591995748056455	21.554342770047473	26.951975362243424	26.901686119652645
38	29.517260999617335	21.44763599135297	22.21533024396401	26.819772765065686
39	27.304371404462664	21.62642227973508	25.458883139851352	25.6103231759509
40	25.687428912764197	21.01408243255407	24.125882709129442	29.172605945552295
41	26.120546886538875	23.472788269915075	23.32103623307425	27.0856286104718
42	25.254886697260186	21.836071474159777	27.075933447588195	25.833108380991842
43	23.012688667590307	24.676934630836016	25.310117749173873	27.000258952399804
44	22.943649504876507	23.72654460620965	23.336449910647143	29.993355978266706
45	24.942803026342844	21.049662481224757	28.386993068406362	25.62054142402604
46	24.08116164673898	22.824068270660916	24.98233045908801	28.112439623512092
47	28.068320915987023	21.78021896819026	23.101494352094747	27.04996576372798
48	23.07760371120705	23.154307659248314	25.80395505091189	27.96413357863275
49	27.303918887336344	22.47728775801455	22.098626077184328	28.12016727746478
50	23.778159308932448	25.723711562447072	23.826470905255377	26.671658223365103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1514.0
1	1455.0
2	1358.5
3	1279.5
4	1181.0
5	967.0
6	757.0
7	743.0
8	831.0
9	895.0
10	924.0
11	948.5
12	964.5
13	995.0
14	1049.5
15	1138.0
16	1226.5
17	1466.5
18	1777.5
19	2144.0
20	2606.5
21	3334.5
22	4235.5
23	5380.5
24	6631.0
25	7981.5
26	9606.5
27	11367.5
28	13404.0
29	15890.0
30	18920.5
31	23485.5
32	29252.0
33	34292.5
34	41232.5
35	50459.0
36	56283.5
37	59684.5
38	63473.0
39	68608.0
40	76300.0
41	89113.0
42	108721.0
43	127142.0
44	136462.0
45	137799.5
46	141973.0
47	149285.0
48	161334.5
49	179208.0
50	201882.0
51	223079.0
52	238203.5
53	247720.5
54	241813.0
55	232896.0
56	231848.0
57	236315.0
58	238134.0
59	224578.0
60	211920.5
61	207284.0
62	199230.0
63	187171.0
64	169217.0
65	152217.0
66	136211.5
67	121778.5
68	106679.5
69	90251.0
70	77536.5
71	68125.5
72	62849.0
73	57631.5
74	48902.5
75	40573.5
76	34962.5
77	27736.5
78	21553.0
79	17821.0
80	14092.0
81	10366.0
82	7703.0
83	5898.5
84	4429.5
85	3290.0
86	2333.0
87	1663.0
88	1152.5
89	784.5
90	554.5
91	393.0
92	310.0
93	276.0
94	176.0
95	59.5
96	29.5
97	26.5
98	14.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	26909.0
25	25215.0
26	23394.0
27	22387.0
28	20521.0
29	19326.0
30	22698.0
31	20195.0
32	22713.0
33	20688.0
34	21692.0
35	24120.0
36	21061.0
37	21782.0
38	24710.0
39	26765.0
40	27079.0
41	27706.0
42	29888.0
43	31098.0
44	32833.0
45	45942.0
46	165587.0
47	343589.0
48	533840.0
49	687074.0
50	519544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.948371102968128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.89658271165804	13.515806367637854
2	12.06936390110812	5.539444835587925
3	6.561134006422001	4.517020141070283
4	4.434580623125643	4.070656073020755
5	3.2978753564290213	3.784043376533323
6	2.4646928242163675	3.393641135096387
7	1.9721831894944382	3.1680874200887574
8	1.5720981932083846	2.886167419844136
9	1.2441833407791656	2.569678292189785
>10	6.947705142374954	26.909058195666336
>50	0.3019326500033087	4.769955805953279
>100	0.1929911622692348	8.538338011543216
>500	0.026290636667162387	4.1290851977198795
>1k	0.015980583072196745	7.475461682032934
>5k	0.0018901764924103676	3.0206980489245248
>10k+	5.155026797482821E-4	1.7128579970906437
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	17943	0.6389147244864968	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	15401	0.5483991345826525	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	10093	0.35939175802497975	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	9134	0.3252436656891078	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	8442	0.300602915015048	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	8261	0.2941578631768907	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	7341	0.261398483668025	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	6952	0.24754696341916768	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	6835	0.24338082493814886	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	6808	0.24241940836560605	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	6803	0.24224136825957962	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	5417	0.19288865086904938	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	5356	0.19071656157552674	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	5254	0.1870845434125873	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA	4429	0.15770792591822405	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	4165	0.1483074083200278	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3940	0.14029560354883783	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	3919	0.13954783510352675	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	3819	0.13598703298299789	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	3804	0.13545291266491855	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	3763	0.1339929837955017	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3570	0.12712063570288096	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	3463	0.12331057743391508	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA	3368	0.11992781541941265	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	3310	0.1178625501895059	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	3261	0.11611775715044674	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	3257	0.11597532506562559	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	3151	0.11220087481786498	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	3142	0.11188040262701737	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	3005	0.1070021037218928	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	3000	0.10682406361586635	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	2970	0.1057558229797077	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	2937	0.10458075827993317	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	2832	0.10084191605337785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.12245598492498815	0.0	0.0	0.0	0.0
2	0.3436886206734474	0.0	0.0	0.0	0.0
3	0.5175269801976673	0.0	0.0	0.0	0.0
4	0.6603151452308753	0.0	0.0	0.0	0.0
5	0.825750011750647	0.0	0.0	0.0	0.0
6	0.9936418317335837	0.0	0.0	0.0	0.0
7	1.149177668358285	0.0	0.0	0.0	0.0
8	1.2616634073457924	0.0	0.0	0.0	0.0
9	1.3617575549538592	0.0	0.0	0.0	0.0
10	1.445080324574235	0.0	0.0	0.0	0.0
11	1.5181835921086928	0.0	0.0	0.0	0.0
12	1.5809961415148222	0.0	0.0	0.0	0.0
13	1.6263251525091549	0.0	0.0	0.0	0.0
14	1.6679865373193428	0.0	0.0	0.0	0.0
15	1.7126746039319802	0.0	0.0	0.0	0.0
16	1.7531965320635987	0.0	0.0	0.0	0.0
17	1.788733337226477	0.0	0.0	0.0	0.0
18	1.8239496701985076	0.0	0.0	0.0	0.0
19	1.8709166501682835	0.0	0.0	0.0	0.0
20	1.8934565275912314	0.0	0.0	0.0	0.0
21	1.9232248333188526	0.0	0.0	0.0	0.0
22	1.9398537792217225	0.0	0.0	0.0	0.0
23	1.956304685018566	0.0	0.0	0.0	0.0
24	1.9711176218399662	0.0	0.0	0.0	0.0
25	1.9873192714883725	0.0	0.0	0.0	0.0
26	2.025989582517316	0.0	0.0	0.0	0.0
27	2.040517655169074	0.0	0.0	0.0	0.0
28	2.05355019093021	0.0	0.0	0.0	0.0
29	2.0681850876455834	0.0	0.0	0.0	0.0
30	2.081324447470335	0.0	0.0	0.0	0.0
31	2.09360921478616	0.0	0.0	0.0	0.0
32	2.1068197906533217	0.0	0.0	0.0	0.0
33	2.1199591504780733	0.0	0.0	0.0	0.0
34	2.132421957899924	0.0	0.0	0.0	0.0
35	2.1448847653217755	0.0	0.0	0.0	0.0
36	2.155602779704567	0.0	0.0	0.0	0.0
37	2.1655018095996375	0.0	0.0	0.0	0.0
38	2.1720536855014108	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCA	225	0.0	160.38478	44
ATTAGTC	295	0.0	126.03428	44
TAAGCCA	730	0.0	106.35728	44
CGTGCGC	1190	0.0	102.00178	44
CTATCAT	630	0.0	81.58101	44
TCGTGCG	2725	0.0	73.84382	43
GAACACA	1365	0.0	68.89656	44
TTAAGCC	495	0.0	68.852715	43
ATGTTCT	715	0.0	67.29431	44
TAATCGC	250	0.0	63.495407	43
CTATACG	70	1.3899774E-4	62.48758	44
ATCCTTG	1115	0.0	61.55253	43
AATCCCG	1105	0.0	59.151966	43
CAATGAA	1645	0.0	58.75	43
AGGTCCC	910	0.0	55.92275	43
ACTTCTA	715	0.0	55.058987	44
ATTAAGC	615	0.0	52.50137	42
TCGCAAA	190	0.0	51.602303	43
CACATGC	1410	0.0	49.63553	44
AACGACA	295	0.0	49.06176	43
>>END_MODULE