##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841186.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3096103
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.45003703042179	38.0	35.0	39.0	32.0	39.0
2	37.03485155371123	39.0	37.0	39.0	33.0	39.0
3	37.16542763596689	39.0	37.0	39.0	33.0	39.0
4	37.399901747454784	39.0	37.0	39.0	33.0	40.0
5	38.15331402088367	40.0	37.0	41.0	33.0	41.0
6	38.248493024941354	40.0	37.0	41.0	33.0	41.0
7	38.25812351850052	40.0	37.0	41.0	33.0	41.0
8	38.173227764063405	40.0	37.0	41.0	33.0	41.0
9	38.1027830792451	40.0	37.0	41.0	33.0	41.0
10	38.062198512129605	40.0	37.0	41.0	33.0	41.0
11	37.907088685357046	39.0	37.0	41.0	33.0	41.0
12	37.83554197001844	39.0	37.0	40.0	33.0	41.0
13	37.74308251372774	39.0	36.0	40.0	33.0	41.0
14	37.66016279174175	39.0	36.0	40.0	33.0	41.0
15	37.650751929118634	39.0	36.0	40.0	33.0	41.0
16	37.62332648493929	39.0	36.0	40.0	33.0	41.0
17	37.62775430920741	39.0	36.0	40.0	33.0	41.0
18	37.52437887240831	39.0	36.0	40.0	32.0	41.0
19	37.36224634645553	39.0	36.0	40.0	32.0	41.0
20	37.29880885745726	39.0	36.0	40.0	32.0	41.0
21	37.1956465918608	38.0	36.0	40.0	31.0	41.0
22	37.1722093870908	38.0	36.0	40.0	31.0	41.0
23	37.17338796545205	38.0	35.0	40.0	31.0	41.0
24	37.161285008928964	38.0	35.0	40.0	31.0	41.0
25	37.1575996019115	38.0	35.0	40.0	31.0	41.0
26	37.14266443880148	38.0	35.0	40.0	31.0	41.0
27	37.21534590313681	39.0	35.0	40.0	32.0	41.0
28	37.24976373372591	39.0	35.0	40.0	32.0	41.0
29	37.2714654144505	39.0	35.0	40.0	32.0	41.0
30	37.3239652305611	39.0	35.0	40.0	32.0	41.0
31	37.28142496074362	39.0	35.0	40.0	32.0	41.0
32	37.288875509236	39.0	35.0	40.0	32.0	41.0
33	37.27169934161972	39.0	35.0	40.0	32.0	41.0
34	37.21233394542521	39.0	35.0	40.0	32.0	41.0
35	37.180354053623866	39.0	35.0	40.0	32.0	41.0
36	37.110208203831526	38.0	35.0	40.0	32.0	41.0
37	37.050184778987656	38.0	35.0	40.0	32.0	41.0
38	36.99859068062652	38.0	35.0	40.0	32.0	41.0
39	36.92388604365329	38.0	35.0	40.0	31.0	41.0
40	36.79365319997496	38.0	35.0	40.0	31.0	41.0
41	36.67861720189339	38.0	35.0	40.0	31.0	41.0
42	36.57110246874946	38.0	35.0	40.0	31.0	41.0
43	36.43632386082314	38.0	34.0	40.0	31.0	41.0
44	36.3213652219907	37.0	34.0	40.0	31.0	41.0
45	36.23054367283532	37.0	34.0	40.0	31.0	41.0
46	36.21268197557001	37.0	34.0	40.0	31.0	41.0
47	36.22404795269031	37.0	34.0	40.0	31.0	41.0
48	36.21501614352169	37.0	34.0	40.0	31.0	41.0
49	36.305533391131334	37.0	34.0	40.0	31.0	41.0
50	36.437350488966366	38.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	9.0
22	29.0
23	95.0
24	221.0
25	540.0
26	1412.0
27	3390.0
28	8049.0
29	18469.0
30	38064.0
31	69429.0
32	112190.0
33	169482.0
34	237299.0
35	280191.0
36	348374.0
37	418107.0
38	483981.0
39	553972.0
40	352799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	58.99874778067784	9.81998983883934	31.18126238048282
2	28.852431589000755	27.589941290712872	27.684544086550094	15.873083033736279
3	29.1998037532989	20.742139392649406	22.95740807072633	27.100648783325358
4	23.430809633917217	21.986090256041223	27.682250881188388	26.900849228853176
5	22.03169597393885	26.271509701066147	24.99290236791218	26.70389195708282
6	23.690555514464474	28.888024720107826	24.47373359348833	22.94768617193937
7	30.532285263119473	27.37215137868475	20.57899882529748	21.51656453289829
8	28.14638272693124	25.108466998675432	24.226164310425073	22.51898596396825
9	27.491979433500756	26.29747782938746	24.566107781297973	21.644434955813807
10	27.674273110423	25.05336547266031	25.16886550608943	22.103495910827256
11	29.42802613478944	24.65741611309443	24.818489565754113	21.096068186362015
12	31.017605034457834	24.01076449975986	23.880794663485034	21.090835802297274
13	28.456805216105536	22.956309916046074	24.88150426520048	23.70538060264791
14	23.852048849796017	25.281523256816712	25.82843658625052	25.037991307136746
15	24.092706218107086	24.892679604005423	26.099228610934453	24.915385566953038
16	24.965383903571684	29.768938565674336	23.126233203481924	22.139444327272056
17	26.638261065604084	30.22845170202671	19.830703306705235	23.30258392566397
18	26.46772410349397	26.51229626404548	22.967323761515686	24.05265587094486
19	25.327548857386205	25.352192740357797	24.720430812540798	24.5998275897152
20	27.05733627078944	23.675407439610375	23.816972497362006	25.450283792238178
21	28.281811037940273	24.255362305453016	24.052203689605932	23.410622967000773
22	27.221994875493483	25.21944521871527	22.86577675225921	24.692783153532037
23	27.127941156996393	26.42651100431736	23.206850676479434	23.23869716220681
24	28.531576630364043	25.103428406613087	22.942389190540496	23.422605772482374
25	26.615622498513357	26.510759527061484	23.66812628107054	23.205491693354617
26	26.36009561309452	25.732400833250463	24.164394657718447	23.743108895936572
27	25.87915036835614	25.000760110460575	24.011096290793148	25.10899323039014
28	26.170682749633624	25.166318146181606	24.124150938875584	24.53884816530919
29	28.04591310405809	24.671594699963826	24.136868477110436	23.145623718867647
30	26.36138980739639	26.696465385517136	23.551026347915368	23.391118459171107
31	26.07658880563527	25.07129963224936	25.18487667081464	23.66723489130073
32	25.356631687943388	25.1904523009493	25.76637691711624	23.686539093991072
33	25.378010930494526	24.189468321743874	26.069322256402216	24.36319849135938
34	24.384577775978524	24.873040242013307	26.62700006816399	24.115381913844175
35	26.90527513916912	24.558373360890265	25.940364599931666	22.595986900008953
36	26.14787130595355	25.126110792277462	24.404490456272004	24.321527445496987
37	25.686769852745577	25.52030802503569	25.10433009182603	23.68859203039271
38	25.36063094801284	26.356514383065104	23.961276458992543	24.32157820992951
39	25.67395273259741	26.10667792968834	25.135780124399982	23.08358921331427
40	25.87672913558869	26.349180735350036	24.59754960506621	23.17654052399506
41	24.80785243358931	27.517794753728055	23.793371689230355	23.88098112345228
42	25.194978387338264	27.354512156868815	24.53261029423961	22.917899161553308
43	24.967466459624323	27.32634838625071	25.023350010226693	22.68283514389828
44	24.72202859091408	26.68374727287739	24.712000395095835	23.8822237411127
45	25.788843371200464	26.843934639538514	24.891545623465976	22.475676365795046
46	25.012920940155176	28.15502953662849	24.007864008415407	22.82418551480093
47	27.449716411439667	27.36759507483068	23.946305717073933	21.236382796655718
48	23.696199379163737	28.51466258705494	24.13959031922369	23.64954771455763
49	25.97419038046939	27.814327765800464	23.18569269366805	23.025789160062093
50	23.73349064277116	30.318825590295674	23.990431293847294	21.957252473085873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1624.0
1	1810.0
2	2093.5
3	2213.0
4	2260.5
5	2218.5
6	2104.5
7	2074.5
8	2062.0
9	2037.5
10	2035.0
11	2109.5
12	2202.5
13	2251.5
14	2370.0
15	2797.5
16	3354.5
17	4197.0
18	5251.0
19	6704.0
20	8372.5
21	10825.5
22	13639.0
23	16966.5
24	20662.0
25	24805.5
26	29840.5
27	37170.0
28	44569.0
29	51008.5
30	58089.5
31	67540.0
32	77964.0
33	87871.0
34	99423.0
35	113055.5
36	123571.5
37	132160.5
38	140581.5
39	148458.0
40	157447.5
41	171106.5
42	189176.5
43	202916.0
44	210097.0
45	204548.5
46	199292.5
47	200473.0
48	204420.5
49	210489.5
50	220413.0
51	224244.0
52	221549.0
53	220381.0
54	210746.5
55	192849.0
56	174894.5
57	163650.5
58	154628.5
59	149476.0
60	152412.0
61	152946.5
62	149835.5
63	142058.5
64	121235.0
65	99957.0
66	86913.0
67	79667.0
68	74385.5
69	68448.5
70	62215.5
71	57184.5
72	53327.5
73	49219.5
74	42165.5
75	32513.0
76	24914.0
77	19095.0
78	15194.5
79	13183.0
80	11082.5
81	8566.0
82	6257.0
83	4833.0
84	4079.0
85	3249.5
86	2285.0
87	1430.5
88	876.0
89	598.0
90	422.5
91	299.5
92	225.5
93	170.5
94	112.5
95	46.5
96	24.0
97	23.0
98	16.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	25429.0
25	23811.0
26	20987.0
27	20792.0
28	19444.0
29	17056.0
30	22566.0
31	17857.0
32	19052.0
33	19020.0
34	18942.0
35	20479.0
36	19989.0
37	20812.0
38	20799.0
39	25187.0
40	24450.0
41	26438.0
42	28840.0
43	31632.0
44	36444.0
45	40800.0
46	111849.0
47	214848.0
48	684512.0
49	396094.0
50	1167974.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.005621360490274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.38973805427254	15.184614191776216
2	11.711103498701744	6.091090466015008
3	6.379376179112381	4.976989242903832
4	4.484632973743521	4.66502668223774
5	3.431741559519122	4.4622285801956325
6	2.632032957268904	4.106859149703994
7	2.0904438826284193	3.805430446089148
8	1.666461062774662	3.466988432841446
9	1.368719288097934	3.2034955999567205
>10	7.467025945072581	32.018336146784364
>50	0.22762627249193662	4.022539796973542
>100	0.130515586330711	6.684187816826019
>500	0.011327413951787442	1.9946417263332559
>1k	0.008840908450173128	4.474256857058249
>5k	2.7627838906791024E-4	0.39351101271972294
>10k+	1.3813919453395512E-4	0.4498038515851426
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	12521	0.404411610337253	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	5491	0.17735198086110185	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	5463	0.1764476181832452	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	4972	0.16058897265368755	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	4959	0.16016908998182552	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	4726	0.1526435005553756	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	4381	0.14150046041749903	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	4203	0.13575129767969604	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	4082	0.1318431589646727	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	4074	0.13158476962814222	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1348146363347731	0.0	0.0	0.0	0.0
2	0.4234032265722426	0.0	0.0	0.0	0.0
3	0.6744930643457275	0.0	0.0	0.0	0.0
4	0.7964528311881097	0.0	0.0	0.0	0.0
5	0.9483857610680265	0.0	0.0	0.0	0.0
6	1.1858132626724627	0.0	0.0	0.0	0.0
7	1.4604488287372868	0.0	0.0	0.0	0.0
8	1.660635967214269	0.0	0.0	0.0	0.0
9	1.841250113449068	0.0	0.0	2.260906694641619E-4	0.0
10	2.0025496567782146	0.0	0.0	2.260906694641619E-4	0.0
11	2.152576965301219	0.0	0.0	2.260906694641619E-4	0.0
12	2.268464582735135	0.0	0.0	2.260906694641619E-4	0.0
13	2.3422024396475183	0.0	0.0	2.260906694641619E-4	0.0
14	2.414648349877249	0.0	0.0	2.260906694641619E-4	0.0
15	2.4827016413859617	0.0	0.0	2.260906694641619E-4	0.0
16	2.544359796815545	0.0	0.0	2.260906694641619E-4	0.0
17	2.6000426988378615	0.0	0.0	2.260906694641619E-4	0.0
18	2.6396085659940898	0.0	0.0	2.260906694641619E-4	0.0
19	2.697649270712247	0.0	0.0	2.260906694641619E-4	0.0
20	2.7255553190575377	0.0	0.0	2.260906694641619E-4	0.0
21	2.7630540715215224	0.0	0.0	2.260906694641619E-4	0.0
22	2.7848556717912807	0.0	0.0	3.229866706630884E-4	0.0
23	2.808207608080222	0.0	0.0	3.229866706630884E-4	0.0
24	2.826908536311615	0.0	0.0	3.229866706630884E-4	0.0
25	2.84812876057418	0.0	0.0	3.229866706630884E-4	0.0
26	2.894057465142471	0.0	0.0	3.229866706630884E-4	0.0
27	2.9122739133678692	0.0	0.0	3.229866706630884E-4	0.0
28	2.930264270923803	0.0	0.0	3.229866706630884E-4	0.0
29	2.947737849806676	0.0	0.0	3.229866706630884E-4	0.0
30	2.964533156681157	0.0	0.0	3.229866706630884E-4	0.0
31	2.980456399544847	0.0	0.0	3.229866706630884E-4	0.0
32	2.9961858504061394	0.0	0.0	3.229866706630884E-4	0.0
33	3.0135302346207475	0.0	0.0	3.229866706630884E-4	0.0
34	3.028742906808979	0.0	0.0	3.229866706630884E-4	0.0
35	3.044859941675067	0.0	0.0	3.229866706630884E-4	0.0
36	3.0616552485495476	0.0	0.0	3.229866706630884E-4	0.0
37	3.0775461927461714	0.0	0.0	3.229866706630884E-4	0.0
38	3.088915323553512	0.0	0.0	3.229866706630884E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTGCG	2145	0.0	66.15177	43
CGTGCGC	1045	0.0	55.21856	44
TAAGCCA	1040	0.0	46.66881	44
CTCGTGC	2450	0.0	45.77783	42
TTAAGCC	970	0.0	38.64892	43
ACTCGTG	3565	0.0	37.30119	41
GACGATC	645	0.0	35.95227	44
CAACTCG	2090	0.0	35.812565	2
ATTTCGT	2570	0.0	35.29682	13
AATTTCG	2580	0.0	35.160015	12
TCGGGTT	1985	0.0	34.785595	26
TTTCGTG	2640	0.0	34.28388	14
CTCGGGT	2085	0.0	33.850353	25
AGGCTCG	2680	0.0	33.806515	22
GAGGGTT	1350	0.0	33.509377	35
TAATCCA	1515	0.0	33.234577	40
TTCGTGG	2720	0.0	33.200756	15
ATAATCC	1495	0.0	33.15289	39
CGGGTTG	2065	0.0	33.13766	27
ATTAAGC	1090	0.0	33.100925	42
>>END_MODULE