##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841185.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41827
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.984842326726756	35.0	31.0	37.0	23.0	39.0
2	34.05400817653668	35.0	32.0	37.0	27.0	39.0
3	34.40887465034547	35.0	33.0	37.0	29.0	39.0
4	34.581920768881346	35.0	33.0	38.0	29.0	39.0
5	34.92985392210773	35.0	33.0	38.0	29.0	40.0
6	34.739761398139954	35.0	33.0	38.0	28.0	40.0
7	34.605661414875556	35.0	33.0	38.0	28.0	40.0
8	34.389293996700694	35.0	32.0	38.0	27.0	40.0
9	34.361943242403235	35.0	32.0	38.0	27.0	40.0
10	34.14708202835489	35.0	32.0	38.0	27.0	40.0
11	34.023979726014296	35.0	32.0	38.0	26.0	40.0
12	34.037463839146966	35.0	32.0	38.0	27.0	40.0
13	33.84486097496832	35.0	32.0	38.0	26.0	40.0
14	33.78638200205609	35.0	31.0	38.0	26.0	40.0
15	33.442489301169104	35.0	31.0	38.0	26.0	40.0
16	33.476343032012814	35.0	31.0	38.0	26.0	40.0
17	33.39792478542568	35.0	31.0	38.0	26.0	40.0
18	33.22786716714084	34.0	31.0	37.0	25.0	40.0
19	33.12623425060368	34.0	31.0	37.0	25.0	40.0
20	33.00829607669687	34.0	31.0	37.0	24.0	40.0
21	32.8173428646568	34.0	30.0	37.0	24.0	40.0
22	32.859540488201404	34.0	30.0	37.0	24.0	40.0
23	33.079111578645374	34.0	31.0	37.0	25.0	40.0
24	33.15408707294331	34.0	31.0	37.0	26.0	40.0
25	33.32326911793468	34.0	31.0	37.0	26.0	40.0
26	33.27941360105891	34.0	31.0	37.0	26.0	40.0
27	33.34035891561665	34.0	31.0	37.0	26.0	40.0
28	33.33398229629053	34.0	31.0	37.0	26.0	40.0
29	33.45963717024479	34.0	31.0	37.0	26.0	40.0
30	33.51312860399624	34.0	32.0	36.0	26.0	40.0
31	33.483737837542364	34.0	32.0	36.0	27.0	40.0
32	33.51667501809476	34.0	32.0	36.0	27.0	40.0
33	33.46678187947984	34.0	32.0	36.0	27.0	39.0
34	33.44042197498126	34.0	32.0	36.0	27.0	39.0
35	33.458796675640876	34.0	32.0	36.0	27.0	39.0
36	33.406534276710865	34.0	32.0	36.0	27.0	39.0
37	33.38608931804466	34.0	32.0	35.0	27.0	39.0
38	33.35118554645563	34.0	32.0	35.0	27.0	39.0
39	33.27189871842727	34.0	32.0	35.0	27.0	39.0
40	33.173121378131036	34.0	32.0	35.0	27.0	39.0
41	33.053703404873325	34.0	31.0	35.0	27.0	39.0
42	33.02952949668614	34.0	31.0	35.0	27.0	38.0
43	33.00343803197703	34.0	31.0	35.0	27.0	38.0
44	32.92844498910675	34.0	31.0	35.0	27.0	38.0
45	32.90479663783821	34.0	31.0	35.0	27.0	37.0
46	32.87418954827281	34.0	31.0	35.0	27.0	37.0
47	32.84648100891305	34.0	31.0	35.0	27.0	37.0
48	32.747297971400066	34.0	31.0	35.0	27.0	37.0
49	32.757250208739215	34.0	31.0	35.0	27.0	37.0
50	32.63452894229887	34.0	31.0	35.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	4.0
22	8.0
23	47.0
24	111.0
25	280.0
26	570.0
27	1194.0
28	1847.0
29	2650.0
30	3317.0
31	4019.0
32	4621.0
33	5306.0
34	6450.0
35	2905.0
36	2634.0
37	2412.0
38	1879.0
39	1252.0
40	319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.970306261505726	14.935328854567622	39.09436488392665
2	21.985798646807087	23.284003155856265	32.701365146914675	22.028833050421976
3	26.253377005283667	20.47959452028594	25.02450570205848	28.242522772371913
4	19.29375762067564	21.54350060965405	32.39773352140961	26.765008248260692
5	21.30442058957133	26.796088650871447	24.845195686996437	27.054295072560784
6	27.245559088626965	23.33181915987281	23.044923135773544	26.377698615726686
7	31.264494226217515	20.527410524302482	21.220742582542375	26.987352666937625
8	24.49135725727401	20.57522652831903	26.107538193033207	28.825878021373754
9	27.192961484208766	22.134028259258372	24.885839290410498	25.787170966122364
10	27.783489133813088	20.613479331532265	25.354436129772633	26.248595404882014
11	27.898247543452793	19.616515647787313	24.86193128840223	27.623305520357665
12	28.30707437779425	19.583044444975734	22.330073875726207	29.779807301503812
13	25.261194921940373	22.13880985966003	24.696966074545152	27.903029143854447
14	25.67958495708514	23.049704736175197	23.248141152843857	28.02256915389581
15	25.88041217395462	24.959954096636146	22.762808712075934	26.396825017333303
16	25.715446960097545	25.120137710091566	23.9725536136945	25.19186171611638
17	26.57135343199369	22.758027111674277	19.44915963372941	31.221459822602625
18	25.851722571544695	22.60023429841968	22.605015898821335	28.943027231214284
19	26.874985057498748	20.96970856145552	23.472876371721615	28.682430009324122
20	26.372917015325033	20.766490544385206	23.664140387787793	29.19645205250197
21	28.854567623783677	23.037750735171063	21.37136299519449	26.736318645850766
22	27.48942070911134	21.651086618691277	22.70303870705525	28.15645396514213
23	29.85631290793028	23.786071198029983	21.737155425921056	24.62046046811868
24	29.160590049489564	23.616324383771246	21.349845793387047	25.87323977335214
25	28.004596127518088	24.273909642088793	21.667807549383923	26.053686681009193
26	30.061686771059666	22.988936340251243	22.646787043280636	24.302589845408455
27	29.487081583301517	24.039709812905688	20.669466717576686	25.803741886216113
28	28.34410611842276	23.728681566856164	21.797367806245617	26.129844508475458
29	30.39953523990599	21.305025218516466	23.330428582745782	24.965010958831762
30	28.593268070269545	25.27557999195387	23.274775378838676	22.85637655893791
31	25.554826719142888	23.964141248496777	24.833278670602382	25.647753361757953
32	26.810867991759924	22.36234062691387	26.167808028506208	24.658983352819998
33	26.328016545315464	20.973453834603507	25.5517466073604	27.146783012720626
34	26.601141407736208	21.536865164005302	26.534847524067562	25.327145904190928
35	27.668851691443287	21.60248156385345	23.6070467049046	27.121620039798668
36	25.38795409953029	22.171353826030085	24.787442773054284	27.653249301385337
37	24.453832226916113	24.354254677127336	24.87024743512372	26.32166566083283
38	27.85190638323978	24.085150762246556	21.90116867580749	26.161774178706175
39	26.25357720542491	23.254945875326612	24.558292895358967	25.93318402388951
40	27.30612115646474	23.385794357009402	22.467462554229076	26.84062193229678
41	24.124576407939323	25.0895594642569	24.09230272712603	26.693561400677744
42	24.890084651223834	25.283811273705624	24.030448192138593	25.79565588293195
43	26.39607463533496	23.69571748055676	22.514102606896092	27.394105277212187
44	23.141339869281047	24.897875816993466	23.376225490196077	28.58455882352941
45	25.483658087596815	21.656073078392556	27.04664652148241	25.813622312528224
46	24.453498671390612	24.559787422497788	24.226749335695306	26.7599645704163
47	25.396649284367022	22.90765191020378	23.565960279596137	28.129738525833055
48	24.709012304622547	23.432823412038577	24.18523445294313	27.67292983039574
49	25.132201502922346	23.534650709713333	21.84803785137768	29.485109935986642
50	24.814237225942573	24.814237225942573	22.41078800110082	27.960737547014038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	365.0
1	331.0
2	184.0
3	72.0
4	69.5
5	49.5
6	26.0
7	22.5
8	27.0
9	29.5
10	28.5
11	27.0
12	24.5
13	20.5
14	27.5
15	36.0
16	39.0
17	42.0
18	47.0
19	53.0
20	64.5
21	80.0
22	85.0
23	113.0
24	157.0
25	170.0
26	161.0
27	149.0
28	164.0
29	234.5
30	304.5
31	385.5
32	502.5
33	615.5
34	716.5
35	821.5
36	948.5
37	1028.0
38	1070.0
39	1173.0
40	1288.0
41	1470.5
42	1765.0
43	1945.0
44	1982.5
45	2102.5
46	2359.0
47	2650.5
48	3021.5
49	3432.0
50	3771.0
51	3823.5
52	3723.5
53	3826.0
54	3820.0
55	3654.5
56	3489.5
57	3289.5
58	3114.0
59	2962.5
60	2852.0
61	2798.0
62	2843.0
63	2751.0
64	2537.0
65	2402.0
66	2176.0
67	1919.5
68	1718.0
69	1492.0
70	1253.5
71	1082.5
72	981.0
73	854.5
74	714.5
75	625.5
76	564.5
77	436.5
78	292.5
79	230.0
80	184.0
81	140.0
82	113.5
83	85.0
84	64.5
85	63.0
86	53.5
87	36.0
88	25.0
89	18.0
90	8.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	923.0
25	863.0
26	756.0
27	762.0
28	654.0
29	584.0
30	697.0
31	666.0
32	625.0
33	603.0
34	522.0
35	534.0
36	498.0
37	539.0
38	453.0
39	569.0
40	594.0
41	507.0
42	519.0
43	583.0
44	585.0
45	566.0
46	1186.0
47	2983.0
48	6091.0
49	7064.0
50	10901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.50759079063763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.76855719390066	32.67028474430392
2	13.20517336822505	12.546919453941236
3	5.903074832670726	8.413225906710977
4	3.401942529314076	6.464723743036795
5	2.2142821196718834	5.259760441819878
6	1.4644456746011776	4.1743371506443205
7	1.0316541693925823	3.4307982881870562
8	0.8555180916914096	3.251488273125015
9	0.6693170952644557	2.8617878403901784
>10	2.4306778722761813	18.45219594998446
>50	0.045292134266015804	1.5683649317426545
>100	0.01006491872578129	0.9061132761135152
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	278	0.6646424558299663	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	101	0.2414708202835489	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	98	0.23429841968106727	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	92	0.21995361847610395	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	90	0.21517201807444952	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	75	0.17931001506204125	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	71	0.1697468142587324	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	63	0.15062041265211465	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	62	0.14822961245128746	Illumina Single End Adapter 1 (100% over 31bp)
AAGAAGGAGATGATCAAGAAGGTGAAAAGAAGAGAAAAGGTGGGAGGAAC	54	0.1291032108446697	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	51	0.12193081024218805	No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG	49	0.11714920984053363	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	47	0.1123676094388792	No Hit
CTGCCAGCGGCGTGAGCTTCGGCCGCCATTTTACAACAGCTCCACTCGC	46	0.10997680923805198	No Hit
ATCTACCTGAGGAAAGGCACTTGCTGCTGAGGCAGG	45	0.10758600903722476	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	43	0.10280440863557033	Illumina Single End Adapter 1 (100% over 32bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.07889640662729816	0.0	0.0	0.0	0.0
2	0.24625242068520334	0.0	0.0	0.0	0.0
3	0.38730963253400913	0.0	0.0	0.0	0.0
4	0.5307576445836422	0.0	0.0	0.0	0.0
5	0.7435388624572644	0.0	0.0	0.0	0.0
6	0.9085040763143424	0.0	0.0	0.0	0.0
7	1.016090085351567	0.0	0.0	0.0	0.0
8	1.0997680923805198	0.0	0.0	0.0	0.0
9	1.1714920984053363	0.0	0.0	0.0	0.0
10	1.2288713032251894	0.0	0.0	0.0	0.0
11	1.2934229086475244	0.0	0.0	0.0	0.0
12	1.346020513065723	0.0	0.0	0.0	0.0
13	1.3699285150739953	0.0	0.0	0.0	0.0
14	1.4344801204963302	0.0	0.0	0.0	0.0
15	1.4751237239103927	0.0	0.0	0.0	0.0
16	1.5181581275252827	0.0	0.0	0.0	0.0
17	1.5683649317426542	0.0	0.0	0.0	0.0
18	1.630525736964162	0.0	0.0	0.0	0.0
19	1.7333301455997323	0.0	0.0	0.0	0.0
20	1.773973749013795	0.0	0.0	0.0	0.0
21	1.807444951825376	0.0	0.0	0.0	0.0
22	1.8265713534319936	0.0	0.0	0.0	0.0
23	1.840916154636957	0.0	0.0	0.0	0.0
24	1.8528701556410931	0.0	0.0	0.0	0.0
25	1.895904559255983	0.0	0.0	0.0	0.0
26	1.9150309608626006	0.0	0.0	0.0	0.0
27	1.9269849618667367	0.0	0.0	0.0	0.0
28	1.9341573624692185	0.0	0.0	0.0	0.0
29	1.9461113634733544	0.0	0.0	0.0	0.0
30	1.9700193654816267	0.0	0.0	0.0	0.0
31	1.972410165682454	0.0	0.0	0.0	0.0
32	1.9795825662849356	0.0	0.0	0.0	0.0
33	1.98436416668659	0.0	0.0	0.0	0.0
34	1.9915365672890717	0.0	0.0	0.0	0.0
35	2.0082721686948624	0.0	0.0	0.0	0.0
36	2.015444569297344	0.0	0.0	0.0	0.0
37	2.029789370502307	0.0	0.0	0.0	0.0
38	2.0369617711047887	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACTGT	10	0.009043619	133.12083	44
CGTGTGC	10	0.009043619	133.12083	44
TTCAGGA	30	2.3474155E-5	88.74722	44
GTTCAGG	40	1.886292E-9	75.55506	43
TCGTGCG	30	2.319177E-6	71.95721	43
TGAACAC	20	0.002981621	64.76148	43
TGTGTTA	25	4.468022E-6	64.543434	42
TGTGCCC	15	0.003033906	64.543434	42
ACCTTAG	45	7.403287E-10	57.37194	42
AGTTCAG	45	7.403287E-10	57.37194	42
CAGTTCA	45	3.274181E-11	57.35907	41
CCGGAAC	15	0.004848096	57.359066	41
CTGTGTT	25	9.025756E-6	57.359066	41
TGGACGC	15	0.004848096	57.359066	41
AACCTTA	40	7.4760464E-10	57.359062	41
CTATACC	15	0.0054623997	55.66028	40
GTGGACG	15	0.0054623997	55.66028	40
ACGCACC	20	2.4236192E-4	55.660275	40
ACAACCT	45	5.2750693E-11	54.613678	39
CCTATAC	15	0.005889614	54.613674	39
>>END_MODULE