FastQCFastQC Report
Tue 24 May 2016
ERR841184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11262632
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG433000.3844572032540884No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG391330.3474587467654097No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC339130.30111078831306926No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA311020.2761521463189066No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT294120.2611467727969803No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG287710.25545538556174086No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG194870.17302349930282726No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA190760.16937426349364873No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG177590.15768072684963869No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG165920.14731902809219016No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG157750.14006495107005182No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA155860.13838683533298435No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC155590.13814710451340326No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC148750.13207392375068278No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC145610.12928594310814737No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG139350.123727739661564No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC133810.11880881840053018No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG131860.1170774291480002No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT130540.1159054118078261No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG124170.11024954024956156Illumina Single End Adapter 1 (100% over 31bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC119360.10597878009332098No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA115280.1023561810418737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG9000.0126.23012544
TAAGCCA53350.0120.4371244
ATGTTCT35750.082.3107844
CGTGCGC45050.074.2070844
TTAAGCC43750.071.871343
TCGTGCG85600.070.4316343
ATTAGTC14000.068.5101544
CGCTACC14500.066.7899444
ACGACAC7550.065.36941544
AATCCCG46250.064.11249543
TTCAGGA59800.064.0009444
CTATCAT22600.058.92143244
ATTAAGC54850.055.60941742
GTTCAGG111300.053.5245443
ATCCTTG46950.053.2347343
AACGACA13150.053.13678743
TCCTTGT43000.049.59212544
AATCGCA3400.049.2991344
CTCGTGC101900.047.3887242
TTTACGC2800.046.5602944
Produced by FastQC (version 0.11.5)