##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841184.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11262632
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.07184874725553	37.0	34.0	39.0	27.0	39.0
2	36.06499280097228	37.0	35.0	39.0	31.0	39.0
3	36.26565788529715	37.0	35.0	39.0	32.0	39.0
4	36.77715519782587	38.0	35.0	39.0	33.0	40.0
5	37.332556457495905	38.0	35.0	40.0	33.0	41.0
6	37.3722526848076	38.0	35.0	40.0	33.0	41.0
7	37.300674567010624	38.0	35.0	40.0	33.0	41.0
8	37.1838381117309	38.0	35.0	40.0	33.0	41.0
9	37.13499846217119	38.0	35.0	40.0	32.0	41.0
10	36.94232476032246	38.0	35.0	40.0	32.0	41.0
11	36.81155408433837	38.0	35.0	40.0	31.0	41.0
12	36.72882803948491	38.0	35.0	40.0	31.0	41.0
13	36.5295476226161	38.0	35.0	40.0	31.0	41.0
14	36.45952038564343	38.0	35.0	40.0	31.0	41.0
15	36.36311982847349	38.0	34.0	40.0	31.0	41.0
16	36.317271220439416	38.0	34.0	40.0	31.0	41.0
17	36.30197195469052	38.0	34.0	40.0	31.0	41.0
18	36.16301367211501	37.0	34.0	40.0	31.0	41.0
19	36.033009424440046	37.0	34.0	40.0	30.0	41.0
20	35.87178139177414	37.0	34.0	40.0	30.0	41.0
21	35.61847630287485	37.0	34.0	40.0	30.0	41.0
22	35.62686892371161	36.0	34.0	39.0	30.0	41.0
23	35.61582443606432	36.0	34.0	39.0	30.0	41.0
24	35.54646409471605	36.0	34.0	39.0	30.0	41.0
25	35.47627078467974	36.0	34.0	39.0	30.0	41.0
26	35.42192829857177	36.0	33.0	39.0	30.0	40.0
27	35.41407150355736	36.0	33.0	39.0	30.0	40.0
28	35.37212578419272	35.0	34.0	39.0	30.0	40.0
29	35.329945530427345	35.0	34.0	39.0	30.0	40.0
30	35.32117347823988	35.0	34.0	39.0	30.0	40.0
31	35.2152596949982	35.0	34.0	39.0	30.0	40.0
32	35.17076053805827	35.0	33.0	39.0	30.0	40.0
33	35.09179535902099	35.0	33.0	39.0	30.0	40.0
34	35.0243835288872	35.0	34.0	38.0	30.0	40.0
35	34.93806287425713	35.0	33.0	38.0	30.0	40.0
36	34.82139572814881	35.0	33.0	38.0	30.0	40.0
37	34.75829927417042	35.0	33.0	37.0	30.0	40.0
38	34.68850182812985	35.0	33.0	37.0	30.0	40.0
39	34.55847532360584	35.0	33.0	37.0	30.0	40.0
40	34.43270471235622	35.0	33.0	37.0	29.0	40.0
41	34.30365756533932	35.0	33.0	37.0	29.0	40.0
42	34.19729566372185	35.0	33.0	36.0	29.0	40.0
43	34.11952698023363	35.0	33.0	36.0	29.0	40.0
44	34.042209624743506	35.0	33.0	36.0	29.0	39.0
45	33.99970728660975	35.0	33.0	36.0	29.0	39.0
46	33.94162663552216	35.0	33.0	36.0	29.0	39.0
47	33.91465638206772	35.0	33.0	36.0	29.0	39.0
48	34.01237915233814	35.0	33.0	36.0	29.0	39.0
49	33.92710184674625	35.0	33.0	36.0	29.0	39.0
50	34.69007664517	35.0	33.0	37.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	19.0
21	96.0
22	387.0
23	1209.0
24	3667.0
25	9417.0
26	22365.0
27	49656.0
28	100341.0
29	189822.0
30	330403.0
31	530333.0
32	795939.0
33	1148369.0
34	1557882.0
35	1304629.0
36	1535791.0
37	1472013.0
38	1158177.0
39	775213.0
40	276902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.24640492559821	20.744236338362114	28.009358736039676
2	29.81535754697481	24.176391450950362	25.617537712321596	20.39071328975323
3	28.127261904677344	18.246054741023237	26.559120461362852	27.06756289293657
4	21.2571981398309	20.033922798862648	29.082074243391776	29.626804817914675
5	22.950692165028567	21.548684179683754	26.39935318849093	29.10127046679675
6	27.408912943262287	21.50553263215916	25.025926444191732	26.059627980386825
7	32.52076424054342	20.118405715466864	21.964430694352792	25.396399349636923
8	27.96737920585526	18.634321000632887	26.778722771018355	26.6195770224935
9	29.425208956485484	19.226189757420823	24.353321674720437	26.995279611373256
10	28.28021016757007	19.949377729823723	25.099683626349506	26.670728476256706
11	31.50520233636329	20.279123032697864	24.548471440778673	23.66720319016017
12	30.849680607516966	18.25687814358136	23.922054809213336	26.971386439688345
13	27.9363118674214	19.784513957305894	24.864747423160058	27.414426752112654
14	28.298545135808396	20.412475520819644	21.854349853568863	29.434629489803093
15	25.97193089501637	21.459486556961107	24.167778899283935	28.400803648738588
16	27.08035741556681	22.661745496079426	23.3152872259344	26.94260986241937
17	30.172432163281194	21.529994054675676	18.681645640202042	29.615928141841092
18	27.446275435439958	21.364490999972297	22.152459567177548	29.036773997410197
19	28.549978370952722	20.597796323275055	24.174473604393715	26.677751701378504
20	30.05958997861246	18.989797411475397	23.994364727534382	26.95624788237776
21	30.00256955922914	22.67310163379217	21.765782634112522	25.558546172866166
22	29.881097065055485	21.488396318018737	22.75883647800976	25.87167013891602
23	29.12821798670151	23.30663027967175	22.63448721400113	24.93066451962561
24	32.23310501488462	22.287507928874884	20.641169843780744	24.83821721245975
25	29.02092138118288	24.290530742784902	21.7890066002778	24.899541275754416
26	30.008772096186163	22.98925307057395	22.363427935918597	24.638546897321284
27	29.565731290381535	22.900123531041046	22.211647406223918	25.322497772353504
28	29.29062187504717	22.16287503022225	23.651499657890156	24.89500343684043
29	31.079202893150832	21.505434755676987	23.67878538378763	23.736576967384547
30	28.28041596637388	23.880895660648925	22.49921924268804	25.33946913028916
31	27.54502070167585	22.16767333097592	26.05248566376431	24.234820303583923
32	27.046892403931977	21.304659109253475	25.524808067910893	26.123640418903655
33	26.421573194747072	20.581550087105434	26.941223094156353	26.055653623991144
34	25.85963061803993	19.797223716062593	29.463293424174086	24.87985224172339
35	28.38254370713253	20.338959719789496	25.125014396647522	26.153482176430458
36	26.703283658814	21.80692114999121	23.65654399704059	27.8332511941542
37	25.64950599642735	22.474287092313386	27.00804866921136	24.868158242047905
38	28.39319222048588	22.55450228932389	22.94312668722364	26.109178802966593
39	26.993888980056695	22.676765741688087	25.679719681887814	24.649625596367404
40	27.611773725735073	22.017005191091364	23.492496817671675	26.878724265501887
41	26.351146781653107	23.24610830700311	23.405954116021636	26.996790795322152
42	25.816824428562686	24.045553948771133	25.159195119097078	24.9784265035691
43	24.930715823409237	24.778147421654996	24.908433365704248	25.38270338923152
44	23.937741433708947	23.821407686982838	25.200441320835804	27.040409558472412
45	25.804756912588957	21.715706919679423	26.786407813864376	25.693128353867245
46	24.897193784671877	22.943336900828225	23.832287387850894	28.327181926649004
47	27.76840497899101	22.44880079893125	24.029397472682955	25.75339674939478
48	24.931307492955437	23.091922499932544	24.22697392390156	27.74979608321046
49	27.790420021452938	22.367641540509005	22.376891962710342	27.465046475327714
50	24.203733508074485	26.207921126380118	23.648330100705426	25.94001526483997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58613.0
1	57857.5
2	32720.0
3	8259.0
4	7972.0
5	6405.0
6	4056.5
7	3288.5
8	3536.5
9	3578.0
10	3592.5
11	3640.0
12	3695.5
13	3744.5
14	3900.5
15	4433.0
16	5170.0
17	6286.0
18	7817.0
19	10234.5
20	13334.0
21	18329.0
22	25607.0
23	35604.0
24	47063.5
25	56162.5
26	61908.5
27	66639.0
28	72582.5
29	80580.5
30	89543.0
31	103670.5
32	122193.0
33	137247.5
34	153881.0
35	177851.5
36	201494.5
37	221483.0
38	242383.5
39	277002.0
40	319316.0
41	371498.5
42	441211.5
43	520880.0
44	577549.0
45	579069.0
46	594085.0
47	643001.0
48	715501.5
49	794936.0
50	871105.0
51	954673.5
52	1021131.0
53	1036079.0
54	986773.5
55	925413.5
56	878531.0
57	860596.5
58	859536.0
59	824552.5
60	780789.0
61	759012.0
62	740414.5
63	703368.5
64	639078.5
65	578470.5
66	522208.5
67	472403.5
68	423862.5
69	370678.0
70	326168.0
71	290345.0
72	262971.0
73	236349.0
74	201965.5
75	169792.5
76	146400.0
77	117232.5
78	92117.5
79	74143.5
80	56662.5
81	42595.5
82	31908.5
83	24009.5
84	18414.0
85	14076.0
86	10283.0
87	7019.0
88	4704.0
89	3453.5
90	2425.0
91	1532.0
92	1085.0
93	888.5
94	591.5
95	294.5
96	212.5
97	199.0
98	110.0
99	19.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	125222.0
25	117239.0
26	109553.0
27	111455.0
28	103834.0
29	90241.0
30	110527.0
31	99999.0
32	101863.0
33	97128.0
34	97734.0
35	106665.0
36	97114.0
37	102084.0
38	108975.0
39	122389.0
40	120888.0
41	125052.0
42	132559.0
43	137444.0
44	138344.0
45	183115.0
46	516244.0
47	1276441.0
48	1957777.0
49	2573769.0
50	2398977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.928559268339207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.94887497908311	11.918579052649806
2	12.742526425461984	5.333654390473193
3	7.239528125030076	4.545386803185017
4	4.877847574169506	4.083452882317245
5	3.42962474056375	3.588855232552546
6	2.624310674332838	3.295382489178602
7	2.031112130471326	2.97557754222483
8	1.5616451700407907	2.6146386797851475
9	1.2695195895959446	2.391229437585875
>10	6.60661360733669	23.700074660343937
>50	0.37103495017370025	5.380991434488715
>100	0.24766243517774894	10.17371896281965
>500	0.026031546791560417	3.7267787223105433
>1k	0.0201767876393328	8.284863742955975
>5k	0.0022670546310501966	3.2743278040584864
>10k+	0.001224209500767106	4.712488163070742
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	43300	0.3844572032540884	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	39133	0.3474587467654097	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	33913	0.30111078831306926	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	31102	0.2761521463189066	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	29412	0.2611467727969803	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	28771	0.25545538556174086	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	19487	0.17302349930282726	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	19076	0.16937426349364873	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	17759	0.15768072684963869	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	16592	0.14731902809219016	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	15775	0.14006495107005182	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	15586	0.13838683533298435	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	15559	0.13814710451340326	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	14875	0.13207392375068278	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	14561	0.12928594310814737	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	13935	0.123727739661564	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	13381	0.11880881840053018	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	13186	0.1170774291480002	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	13054	0.1159054118078261	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	12417	0.11024954024956156	Illumina Single End Adapter 1 (100% over 31bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	11936	0.10597878009332098	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	11528	0.1023561810418737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.22001961885996096	0.0	0.0	0.0	0.0
2	0.6173778917752085	0.0	0.0	0.0	0.0
3	0.8091092739246031	0.0	0.0	0.0	0.0
4	0.987992859928301	0.0	0.0	0.0	0.0
5	1.3006107275812617	0.0	0.0	0.0	0.0
6	1.5179045182333935	0.0	0.0	0.0	0.0
7	1.6881045212167103	0.0	0.0	0.0	0.0
8	1.821741134754292	0.0	0.0	0.0	0.0
9	1.9410294147939842	0.0	0.0	0.0	0.0
10	2.053480927015994	0.0	0.0	0.0	0.0
11	2.164520691078249	0.0	0.0	0.0	0.0
12	2.2493410066137294	0.0	0.0	0.0	0.0
13	2.31532913443323	0.0	0.0	0.0	0.0
14	2.383980938025854	4.528248814309124E-4	0.0	0.0	0.0
15	2.4660576675150176	6.659189432807536E-4	0.0	0.0	0.0
16	2.522385531197326	6.659189432807536E-4	0.0	0.0	0.0
17	2.57877554731434	6.659189432807536E-4	0.0	0.0	0.0
18	2.6333631428248743	6.659189432807536E-4	0.0	0.0	0.0
19	2.7738631609378697	6.659189432807536E-4	0.0	0.0	0.0
20	2.8128593742563903	6.659189432807536E-4	0.0	0.0	0.0
21	2.8842636428145747	6.659189432807536E-4	0.0	0.0	0.0
22	2.9115751984083293	6.659189432807536E-4	0.0	0.0	0.0
23	2.9401475605346956	6.659189432807536E-4	0.0	0.0	0.0
24	2.9651683549635646	6.659189432807536E-4	0.0	0.0	0.0
25	3.000399906522738	6.659189432807536E-4	0.0	0.0	0.0
26	3.1052688217105913	6.659189432807536E-4	0.0	0.0	0.0
27	3.1289932939298737	6.659189432807536E-4	0.0	0.0	0.0
28	3.1525224299257935	6.659189432807536E-4	0.0	0.0	0.0
29	3.173618742048928	6.659189432807536E-4	0.0	0.0	0.0
30	3.193081333031213	6.659189432807536E-4	2.663675773123014E-5	0.0	0.0
31	3.210679350972313	6.659189432807536E-4	2.663675773123014E-5	0.0	0.0
32	3.227131988330969	6.659189432807536E-4	2.663675773123014E-5	0.0	0.0
33	3.2430163748580263	6.659189432807536E-4	2.663675773123014E-5	0.0	0.0
34	3.256823094282047	6.659189432807536E-4	2.663675773123014E-5	0.0	0.0
35	3.2713046115685924	6.747978625244969E-4	2.663675773123014E-5	0.0	0.0
36	3.2846496271919388	6.747978625244969E-4	2.663675773123014E-5	0.0	0.0
37	3.2958903389545178	6.747978625244969E-4	2.663675773123014E-5	0.0	0.0
38	3.303872487354643	6.747978625244969E-4	2.663675773123014E-5	8.878919243743381E-6	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	900	0.0	126.230125	44
TAAGCCA	5335	0.0	120.43712	44
ATGTTCT	3575	0.0	82.31078	44
CGTGCGC	4505	0.0	74.20708	44
TTAAGCC	4375	0.0	71.8713	43
TCGTGCG	8560	0.0	70.43163	43
ATTAGTC	1400	0.0	68.51015	44
CGCTACC	1450	0.0	66.78994	44
ACGACAC	755	0.0	65.369415	44
AATCCCG	4625	0.0	64.112495	43
TTCAGGA	5980	0.0	64.00094	44
CTATCAT	2260	0.0	58.921432	44
ATTAAGC	5485	0.0	55.609417	42
GTTCAGG	11130	0.0	53.52454	43
ATCCTTG	4695	0.0	53.23473	43
AACGACA	1315	0.0	53.136787	43
TCCTTGT	4300	0.0	49.592125	44
AATCGCA	340	0.0	49.29913	44
CTCGTGC	10190	0.0	47.38872	42
TTTACGC	280	0.0	46.56029	44
>>END_MODULE